Standardization of a Two-step Real-time Polymerase Chain Reaction Based Method for Species-specific Detection of Medically Important Aspergillus Species

Purpose: Standardization of Aspergillus polymerase chain reaction (PCR) poses two technical challenges (a) standardization of DNA extraction, (b) optimization of PCR against various medically important Aspergillus species. Many cases of aspergillosis go undiagnosed because of relative insensitivity...

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Veröffentlicht in:	Indian journal of medical microbiology 2017-07, Vol.35 (3), p.381-388
Hauptverfasser:	Das, P., Pandey, P., Harishankar, A., Chandy, M., Bhattacharya, S., Chakrabarti, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	28S rDNA Aspergillosis Aspergillosis - diagnosis Aspergillosis - microbiology Aspergillus - classification Aspergillus - genetics Aspergillus - isolation & purification Aspergillus fumigatus Aspergillus species Assaying Bacteria beta-tubulin gene Blood Calcium-binding protein Calmodulin calmodulin gene Cancer therapies Clinical medicine Cytomegalovirus Deoxyribonucleic acid Diagnostic systems DNA DNA extraction DNA, Fungal - genetics DNA, Fungal - isolation & purification Ethylenediaminetetraacetic acids Fungi Hematology Humans Laboratories Methods Microscopy Molecular Diagnostic Techniques - methods Molecular Diagnostic Techniques - standards Nucleic acids Optimization Polymerase chain reaction Real time real-time polymerase chain reaction Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards RNA, Ribosomal, 28S - genetics Sensitivity analysis Sensitivity and Specificity Species Standardization Time Factors Tubulin Tubulin - genetics Viruses Yeast
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