Comparative analysis of different DNA extraction protocols: a fast, universal maxi-preparation of high quality plant DNA for genetic evaluation and phylogenetic studies
Four DNA extraction protocols were compared for ability to produce DNA from the leaves or needles of several species: oak, elm, pine, fir, poplar and maize (fresh materials) and rhododendron (silica dried or frozen material). With the exception of maize and poplar, the species are known to be diffic...
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| Veröffentlicht in: | Plant molecular biology reporter 1998-03, Vol.16 (1), p.69 |
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| creator | Csaikl, U.M. (Austrian Research Centre Seibersdorf, Austria.) Bastian, H Brettscheider, R Gauch, S Meir, A Schauerte, M Scholz, F Sperisen, C Vornam, B Ziegenhagen, B |
| description | Four DNA extraction protocols were compared for ability to produce DNA from the leaves or needles of several species: oak, elm, pine, fir, poplar and maize (fresh materials) and rhododendron (silica dried or frozen material). With the exception of maize and poplar, the species are known to be difficult for DNA extraction. Two protocols represented classical procedures for lysis and purification, and the other two were a combination of classical lysis followed by anion exchange chromatography. The DNA obtained from all procedures was quantified and tested by PCR and Southern hybridisation.Test results indicated superiority of one of the four protocols; a combination of CTAB lysis followed by anion exchange chromatography which enabled DNA extraction from all seven species. A second protocol also produced DNA from leaves or needles of all species investigated and was well suited for PCR applications but not Southern hybridisations. The remaining protocols produced DNA from some but not all species tested. Abbreviations: CTAB, hexadecyltrimethylammonium bromide; EtOH, Ethanol; TBE, tris-borate-EDTA. |
| doi_str_mv | 10.1023/A:1007428009556 |
| format | Article |
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(Austrian Research Centre Seibersdorf, Austria.) ; Bastian, H ; Brettscheider, R ; Gauch, S ; Meir, A ; Schauerte, M ; Scholz, F ; Sperisen, C ; Vornam, B ; Ziegenhagen, B</creator><creatorcontrib>Csaikl, U.M. (Austrian Research Centre Seibersdorf, Austria.) ; Bastian, H ; Brettscheider, R ; Gauch, S ; Meir, A ; Schauerte, M ; Scholz, F ; Sperisen, C ; Vornam, B ; Ziegenhagen, B</creatorcontrib><description>Four DNA extraction protocols were compared for ability to produce DNA from the leaves or needles of several species: oak, elm, pine, fir, poplar and maize (fresh materials) and rhododendron (silica dried or frozen material). With the exception of maize and poplar, the species are known to be difficult for DNA extraction. Two protocols represented classical procedures for lysis and purification, and the other two were a combination of classical lysis followed by anion exchange chromatography. The DNA obtained from all procedures was quantified and tested by PCR and Southern hybridisation.Test results indicated superiority of one of the four protocols; a combination of CTAB lysis followed by anion exchange chromatography which enabled DNA extraction from all seven species. A second protocol also produced DNA from leaves or needles of all species investigated and was well suited for PCR applications but not Southern hybridisations. The remaining protocols produced DNA from some but not all species tested. Abbreviations: CTAB, hexadecyltrimethylammonium bromide; EtOH, Ethanol; TBE, tris-borate-EDTA.</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>DOI: 10.1023/A:1007428009556</identifier><language>eng</language><publisher>New York: Springer Nature B.V</publisher><subject>Abbreviations ; ABIES ALBA ; ANALYTICAL METHODS ; Anion exchange ; Anion exchanging ; Chromatography ; Corn ; Deoxyribonucleic acid ; DNA ; DNA FINGERPRINTING ; Elm ; EMPREINTE ADN ; Ethanol ; Ethylenediaminetetraacetic acids ; FEUILLE ; GENETIC ANALYSIS ; GENETICA ; GENETICS ; GENETIQUE ; HEXADECYLTRIMETHYLAMMONIUM BROMIDE ; HIBRIDOS ; HOJAS ; HUELLAS GENETICAS ADN ; HYBRIDE ; HYBRIDS ; ISOLATION TECHNIQUES ; LEAVES ; Lysis ; Phylogeny ; Pine ; PINE NEEDLES ; PINUS ; PINUS SYLVESTRIS ; Polymerase chain reaction ; Poplar ; POPULUS TREMULA ; POPULUS TREMULOIDES ; PURIFICACION ; PURIFICATION ; QUERCUS ROBUR ; RAPID METHODS ; RHODODENDRON ; RHODODENDRON LUTEUM ; Silica ; Silicon dioxide ; Species ; TECHNIQUE ANALYTIQUE ; TECHNIQUE DE L'ISOLEMENT ; TECNICAS ANALITICAS ; TECNICAS DE AISLAMIENTO ; ULMUS GLABRA ; ZEA MAYS</subject><ispartof>Plant molecular biology reporter, 1998-03, Vol.16 (1), p.69</ispartof><rights>Kluwer Academic Publishers 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c249t-cc890961eb7cf794998bd5d666c276f8fda47a60ac901ced96eb51ce71aeb6843</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids></links><search><creatorcontrib>Csaikl, U.M. (Austrian Research Centre Seibersdorf, Austria.)</creatorcontrib><creatorcontrib>Bastian, H</creatorcontrib><creatorcontrib>Brettscheider, R</creatorcontrib><creatorcontrib>Gauch, S</creatorcontrib><creatorcontrib>Meir, A</creatorcontrib><creatorcontrib>Schauerte, M</creatorcontrib><creatorcontrib>Scholz, F</creatorcontrib><creatorcontrib>Sperisen, C</creatorcontrib><creatorcontrib>Vornam, B</creatorcontrib><creatorcontrib>Ziegenhagen, B</creatorcontrib><title>Comparative analysis of different DNA extraction protocols: a fast, universal maxi-preparation of high quality plant DNA for genetic evaluation and phylogenetic studies</title><title>Plant molecular biology reporter</title><description>Four DNA extraction protocols were compared for ability to produce DNA from the leaves or needles of several species: oak, elm, pine, fir, poplar and maize (fresh materials) and rhododendron (silica dried or frozen material). With the exception of maize and poplar, the species are known to be difficult for DNA extraction. Two protocols represented classical procedures for lysis and purification, and the other two were a combination of classical lysis followed by anion exchange chromatography. The DNA obtained from all procedures was quantified and tested by PCR and Southern hybridisation.Test results indicated superiority of one of the four protocols; a combination of CTAB lysis followed by anion exchange chromatography which enabled DNA extraction from all seven species. A second protocol also produced DNA from leaves or needles of all species investigated and was well suited for PCR applications but not Southern hybridisations. The remaining protocols produced DNA from some but not all species tested. Abbreviations: CTAB, hexadecyltrimethylammonium bromide; EtOH, Ethanol; TBE, tris-borate-EDTA.</description><subject>Abbreviations</subject><subject>ABIES ALBA</subject><subject>ANALYTICAL METHODS</subject><subject>Anion exchange</subject><subject>Anion exchanging</subject><subject>Chromatography</subject><subject>Corn</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA FINGERPRINTING</subject><subject>Elm</subject><subject>EMPREINTE ADN</subject><subject>Ethanol</subject><subject>Ethylenediaminetetraacetic acids</subject><subject>FEUILLE</subject><subject>GENETIC ANALYSIS</subject><subject>GENETICA</subject><subject>GENETICS</subject><subject>GENETIQUE</subject><subject>HEXADECYLTRIMETHYLAMMONIUM BROMIDE</subject><subject>HIBRIDOS</subject><subject>HOJAS</subject><subject>HUELLAS GENETICAS ADN</subject><subject>HYBRIDE</subject><subject>HYBRIDS</subject><subject>ISOLATION TECHNIQUES</subject><subject>LEAVES</subject><subject>Lysis</subject><subject>Phylogeny</subject><subject>Pine</subject><subject>PINE NEEDLES</subject><subject>PINUS</subject><subject>PINUS SYLVESTRIS</subject><subject>Polymerase chain reaction</subject><subject>Poplar</subject><subject>POPULUS TREMULA</subject><subject>POPULUS TREMULOIDES</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>QUERCUS ROBUR</subject><subject>RAPID METHODS</subject><subject>RHODODENDRON</subject><subject>RHODODENDRON LUTEUM</subject><subject>Silica</subject><subject>Silicon dioxide</subject><subject>Species</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECHNIQUE DE L'ISOLEMENT</subject><subject>TECNICAS ANALITICAS</subject><subject>TECNICAS DE AISLAMIENTO</subject><subject>ULMUS GLABRA</subject><subject>ZEA MAYS</subject><issn>0735-9640</issn><issn>1572-9818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNo1kMtKAzEUhoMoWKtrV0LAraPJXHLprtQrFF1o1-U0k7Qp08k0yZT2jXxMA62rc-B8_wfnR-iWkkdK8uJpPKKE8DIXhMiqYmdoQCueZ1JQcY4GhBdVJllJLtFVCGuSUCLEAP1O3KYDD9HuNIYWmkOwATuDa2uM9rqN-PlzjPU-elDRuhZ33kWnXBNGGLCBEB9w36a0D9DgDext1nl9VCY6mVZ2ucLbHhobD7hr4KQ0zuOlbnW0CusdNP0xAG2Nu9Whcf-3EPva6nCNLgw0Qd-c5hDNXl9-Ju_Z9OvtYzKeZiovZcyUEpJIRvWCK8NlKaVY1FXNGFM5Z0aYGkoOjICShCpdS6YXVVo4Bb1goiyG6P7oTX9uex3ifO16n4oJcyqrMtXIhEjU3ZEy4Oaw9DbMZ99USklIzqui-AMsknul</recordid><startdate>19980301</startdate><enddate>19980301</enddate><creator>Csaikl, U.M. 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(Austrian Research Centre Seibersdorf, Austria.)</creatorcontrib><creatorcontrib>Bastian, H</creatorcontrib><creatorcontrib>Brettscheider, R</creatorcontrib><creatorcontrib>Gauch, S</creatorcontrib><creatorcontrib>Meir, A</creatorcontrib><creatorcontrib>Schauerte, M</creatorcontrib><creatorcontrib>Scholz, F</creatorcontrib><creatorcontrib>Sperisen, C</creatorcontrib><creatorcontrib>Vornam, B</creatorcontrib><creatorcontrib>Ziegenhagen, B</creatorcontrib><collection>AGRIS</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Csaikl, U.M. (Austrian Research Centre Seibersdorf, Austria.)</au><au>Bastian, H</au><au>Brettscheider, R</au><au>Gauch, S</au><au>Meir, A</au><au>Schauerte, M</au><au>Scholz, F</au><au>Sperisen, C</au><au>Vornam, B</au><au>Ziegenhagen, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative analysis of different DNA extraction protocols: a fast, universal maxi-preparation of high quality plant DNA for genetic evaluation and phylogenetic studies</atitle><jtitle>Plant molecular biology reporter</jtitle><date>1998-03-01</date><risdate>1998</risdate><volume>16</volume><issue>1</issue><spage>69</spage><pages>69-</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><abstract>Four DNA extraction protocols were compared for ability to produce DNA from the leaves or needles of several species: oak, elm, pine, fir, poplar and maize (fresh materials) and rhododendron (silica dried or frozen material). With the exception of maize and poplar, the species are known to be difficult for DNA extraction. Two protocols represented classical procedures for lysis and purification, and the other two were a combination of classical lysis followed by anion exchange chromatography. The DNA obtained from all procedures was quantified and tested by PCR and Southern hybridisation.Test results indicated superiority of one of the four protocols; a combination of CTAB lysis followed by anion exchange chromatography which enabled DNA extraction from all seven species. A second protocol also produced DNA from leaves or needles of all species investigated and was well suited for PCR applications but not Southern hybridisations. The remaining protocols produced DNA from some but not all species tested. Abbreviations: CTAB, hexadecyltrimethylammonium bromide; EtOH, Ethanol; TBE, tris-borate-EDTA.</abstract><cop>New York</cop><pub>Springer Nature B.V</pub><doi>10.1023/A:1007428009556</doi></addata></record> |
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| ispartof | Plant molecular biology reporter, 1998-03, Vol.16 (1), p.69 |
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| subjects | Abbreviations ABIES ALBA ANALYTICAL METHODS Anion exchange Anion exchanging Chromatography Corn Deoxyribonucleic acid DNA DNA FINGERPRINTING Elm EMPREINTE ADN Ethanol Ethylenediaminetetraacetic acids FEUILLE GENETIC ANALYSIS GENETICA GENETICS GENETIQUE HEXADECYLTRIMETHYLAMMONIUM BROMIDE HIBRIDOS HOJAS HUELLAS GENETICAS ADN HYBRIDE HYBRIDS ISOLATION TECHNIQUES LEAVES Lysis Phylogeny Pine PINE NEEDLES PINUS PINUS SYLVESTRIS Polymerase chain reaction Poplar POPULUS TREMULA POPULUS TREMULOIDES PURIFICACION PURIFICATION QUERCUS ROBUR RAPID METHODS RHODODENDRON RHODODENDRON LUTEUM Silica Silicon dioxide Species TECHNIQUE ANALYTIQUE TECHNIQUE DE L'ISOLEMENT TECNICAS ANALITICAS TECNICAS DE AISLAMIENTO ULMUS GLABRA ZEA MAYS |
| title | Comparative analysis of different DNA extraction protocols: a fast, universal maxi-preparation of high quality plant DNA for genetic evaluation and phylogenetic studies |
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