Efficient preparation of maritime pine (Pinus pinaster) protoplasts suitable for transgene expression analysis

Establishment of protoplast techniques for conifers is of crucial importance to forest biotechnology. Protoplasts can be used to regenerate whole trees and to study transcriptional regulation in woody plants by promoter analysis. Here we describe a technique for isolating protoplasts fromPinus pinas...

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Veröffentlicht in:	Plant molecular biology reporter 2001-12, Vol.19 (4), p.361-366
Hauptverfasser:	Gomez-Maldonado, J, Crespillo, R, Avila, C, Canovas, F.M
Format:	Artikel
Sprache:	eng
Schlagworte:	beta-glucuronidase Biotechnology Coniferous trees Conifers cytosol Deoxyribonucleic acid differential staining DNA Electroporation enzyme activity Fluorescein Fluorescein diacetate fluorescent dyes forest trees Gene expression Gene regulation gene transfer Glutamate-ammonia ligase Glutamine isolation methodology molecular sequence data Pine Pine trees Pinus pinaster Plants (botany) Plasmids promoter regions Promoters Protoplasts Reporter gene seedlings Shoots Transcription transcription (genetics) Transgenes transient gene expression Viability Woody plants
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