Angiosperm DNA contamination by endophytic fungi: detection and methods of avoidance
PCR primers with broad applicability are useful in many molecular-based studies; however, their universality can compromise results when DNA contaminants also are amplified. Eighty-one templates ofDahlia (Asteraceae), primarily extracted from native Mexican populations, were tested for the presence...
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| Veröffentlicht in: | Plant molecular biology reporter 2001-09, Vol.19 (3), p.249-260 |
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| creator | Saar, D.E Polans, N.O Sorensen, P.D Duvall, M.R |
| description | PCR primers with broad applicability are useful in many molecular-based studies; however, their universality can compromise results when DNA contaminants also are amplified. Eighty-one templates ofDahlia (Asteraceae), primarily extracted from native Mexican populations, were tested for the presence of fungal contaminants; out of these, almost 1 in 7 templates (13.6%) was contaminated. In a second survey across 12 angiosperm families using material collected in Illinois, fungal DNA contaminated over 60% of the templates analyzed. Endophytic fungi often are symptomless symbionts living within the above-ground tissues of their angiosperm hosts and are not affected by surface sterilization techniques. Recent studies have revealed their widespread occurrence and broad host range. We also present field strategies for obtaining plant material to reduce the possibility of collecting infected leaves and a simple screening test for detecting fungal DNA in angiosperm templates. |
| doi_str_mv | 10.1007/BF02772897 |
| format | Article |
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Eighty-one templates ofDahlia (Asteraceae), primarily extracted from native Mexican populations, were tested for the presence of fungal contaminants; out of these, almost 1 in 7 templates (13.6%) was contaminated. In a second survey across 12 angiosperm families using material collected in Illinois, fungal DNA contaminated over 60% of the templates analyzed. Endophytic fungi often are symptomless symbionts living within the above-ground tissues of their angiosperm hosts and are not affected by surface sterilization techniques. Recent studies have revealed their widespread occurrence and broad host range. We also present field strategies for obtaining plant material to reduce the possibility of collecting infected leaves and a simple screening test for detecting fungal DNA in angiosperm templates.</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>DOI: 10.1007/BF02772897</identifier><language>eng</language><publisher>New York: Springer Nature B.V</publisher><subject>Ascomycota ; Basidiomycota ; Contaminants ; Contamination ; Dahlia ; Deoxyribonucleic acid ; DNA ; DNA fingerprinting ; Endophytes ; Fungi ; Host plants ; Host range ; internal transcribed spacers ; leaves ; Liliopsida ; Magnoliophyta ; Magnoliopsida ; microbial contamination ; molecular sequence data ; Plant tissues ; polymerase chain reaction ; Primers ; Sterilization ; Symbionts ; Zygomycota</subject><ispartof>Plant molecular biology reporter, 2001-09, Vol.19 (3), p.249-260</ispartof><rights>Springer 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c281t-1bcfd9a2f077da695573fc0d55ae237afeaa6b6ef3cd92a0e07335289baa4503</citedby><cites>FETCH-LOGICAL-c281t-1bcfd9a2f077da695573fc0d55ae237afeaa6b6ef3cd92a0e07335289baa4503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Saar, D.E</creatorcontrib><creatorcontrib>Polans, N.O</creatorcontrib><creatorcontrib>Sorensen, P.D</creatorcontrib><creatorcontrib>Duvall, M.R</creatorcontrib><title>Angiosperm DNA contamination by endophytic fungi: detection and methods of avoidance</title><title>Plant molecular biology reporter</title><description>PCR primers with broad applicability are useful in many molecular-based studies; however, their universality can compromise results when DNA contaminants also are amplified. Eighty-one templates ofDahlia (Asteraceae), primarily extracted from native Mexican populations, were tested for the presence of fungal contaminants; out of these, almost 1 in 7 templates (13.6%) was contaminated. In a second survey across 12 angiosperm families using material collected in Illinois, fungal DNA contaminated over 60% of the templates analyzed. Endophytic fungi often are symptomless symbionts living within the above-ground tissues of their angiosperm hosts and are not affected by surface sterilization techniques. Recent studies have revealed their widespread occurrence and broad host range. We also present field strategies for obtaining plant material to reduce the possibility of collecting infected leaves and a simple screening test for detecting fungal DNA in angiosperm templates.</description><subject>Ascomycota</subject><subject>Basidiomycota</subject><subject>Contaminants</subject><subject>Contamination</subject><subject>Dahlia</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA fingerprinting</subject><subject>Endophytes</subject><subject>Fungi</subject><subject>Host plants</subject><subject>Host range</subject><subject>internal transcribed spacers</subject><subject>leaves</subject><subject>Liliopsida</subject><subject>Magnoliophyta</subject><subject>Magnoliopsida</subject><subject>microbial contamination</subject><subject>molecular sequence data</subject><subject>Plant tissues</subject><subject>polymerase chain reaction</subject><subject>Primers</subject><subject>Sterilization</subject><subject>Symbionts</subject><subject>Zygomycota</subject><issn>0735-9640</issn><issn>1572-9818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpF0E1LAzEQBuAgCtbqxT9gwJuwOkk2m423Wq0KRQ_W8zKbj3aL3azJVui_d7WCpznMwwzvS8g5g2sGoG7uZsCV4qVWB2TEpOKZLll5SEaghMx0kcMxOUlpDQOGshyRxaRdNiF1Lm7o_cuEmtD2uGla7JvQ0npHXWtDt9r1jaF-O9hbal3vzO8aW0s3rl8Fm2jwFL9CY7E17pQcefxI7uxvjsli9rCYPmXz18fn6WSeGV6yPmO18VYj96CUxUJLqYQ3YKVEx4VC7xCLunBeGKs5ghtCCDmEqxFzCWJMLvdnuxg-ty711TpsYzt8rJiWeVFCLotBXe2ViSGl6HzVxWaDcVcxqH5Kq_5LG_DFHnsMFS5jk6r3Nw5MAOgcBAfxDcChaDg</recordid><startdate>20010901</startdate><enddate>20010901</enddate><creator>Saar, D.E</creator><creator>Polans, N.O</creator><creator>Sorensen, P.D</creator><creator>Duvall, M.R</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20010901</creationdate><title>Angiosperm DNA contamination by endophytic fungi: detection and methods of avoidance</title><author>Saar, D.E ; Polans, N.O ; Sorensen, P.D ; Duvall, M.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c281t-1bcfd9a2f077da695573fc0d55ae237afeaa6b6ef3cd92a0e07335289baa4503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Ascomycota</topic><topic>Basidiomycota</topic><topic>Contaminants</topic><topic>Contamination</topic><topic>Dahlia</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA fingerprinting</topic><topic>Endophytes</topic><topic>Fungi</topic><topic>Host plants</topic><topic>Host range</topic><topic>internal transcribed spacers</topic><topic>leaves</topic><topic>Liliopsida</topic><topic>Magnoliophyta</topic><topic>Magnoliopsida</topic><topic>microbial contamination</topic><topic>molecular sequence data</topic><topic>Plant tissues</topic><topic>polymerase chain reaction</topic><topic>Primers</topic><topic>Sterilization</topic><topic>Symbionts</topic><topic>Zygomycota</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saar, D.E</creatorcontrib><creatorcontrib>Polans, N.O</creatorcontrib><creatorcontrib>Sorensen, P.D</creatorcontrib><creatorcontrib>Duvall, M.R</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saar, D.E</au><au>Polans, N.O</au><au>Sorensen, P.D</au><au>Duvall, M.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiosperm DNA contamination by endophytic fungi: detection and methods of avoidance</atitle><jtitle>Plant molecular biology reporter</jtitle><date>2001-09-01</date><risdate>2001</risdate><volume>19</volume><issue>3</issue><spage>249</spage><epage>260</epage><pages>249-260</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><abstract>PCR primers with broad applicability are useful in many molecular-based studies; however, their universality can compromise results when DNA contaminants also are amplified. Eighty-one templates ofDahlia (Asteraceae), primarily extracted from native Mexican populations, were tested for the presence of fungal contaminants; out of these, almost 1 in 7 templates (13.6%) was contaminated. In a second survey across 12 angiosperm families using material collected in Illinois, fungal DNA contaminated over 60% of the templates analyzed. Endophytic fungi often are symptomless symbionts living within the above-ground tissues of their angiosperm hosts and are not affected by surface sterilization techniques. Recent studies have revealed their widespread occurrence and broad host range. We also present field strategies for obtaining plant material to reduce the possibility of collecting infected leaves and a simple screening test for detecting fungal DNA in angiosperm templates.</abstract><cop>New York</cop><pub>Springer Nature B.V</pub><doi>10.1007/BF02772897</doi><tpages>12</tpages></addata></record> |
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| subjects | Ascomycota Basidiomycota Contaminants Contamination Dahlia Deoxyribonucleic acid DNA DNA fingerprinting Endophytes Fungi Host plants Host range internal transcribed spacers leaves Liliopsida Magnoliophyta Magnoliopsida microbial contamination molecular sequence data Plant tissues polymerase chain reaction Primers Sterilization Symbionts Zygomycota |
| title | Angiosperm DNA contamination by endophytic fungi: detection and methods of avoidance |
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