Rapid detection of viruses, transgenes, and mRNAs in small plant leaf samples
A simple method for the detection of DNA and RNA from small tissue samples (
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| Veröffentlicht in: | Plant molecular biology reporter 2001-12, Vol.19 (4), p.329-340 |
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| container_title | Plant molecular biology reporter |
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| creator | Myslik, J.T Nassuth, A |
| description | A simple method for the detection of DNA and RNA from small tissue samples ( |
| doi_str_mv | 10.1007/BF02772831 |
| format | Article |
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Stabs of fresh leaves from wheat, clover, tobacco, broad bean, grape, tomato, lettuce, or asparagus were taken using glass microcapillaries, combined with RNase inhibitor and subjected to RT-PCR either directly or after a DNase treatment. This method was used to successfully detect the presence of (1) virus in infected plants, (2) transgenes such asneomycin phosphotransferase II in transformed plants and (3) various plant genes including RubiscoL and 1,3-β-D-glucanase. With the described DNase treatment, the technique was shown to effectively detect RNA and, therefore, can be used to localize and study patterns of gene expression, virus distribution, and/or transgene expression. The benefits of the described tissue stab technique are that it is quick, it requires little manipulation, and is applicable to a variety of plants.</description><identifier>ISSN: 0735-9640</identifier><identifier>EISSN: 1572-9818</identifier><identifier>DOI: 10.1007/BF02772831</identifier><language>eng</language><publisher>New York: Springer Nature B.V</publisher><subject>Asparagus ; Asparagus officinalis ; Clover ; Deoxyribonuclease ; Deoxyribonucleic acid ; detection ; DNA ; DNA fingerprinting ; Gene expression ; Lactuca sativa ; Leaves ; messenger RNA ; molecular sequence data ; neomycin ; Nicotiana tabacum ; nucleotide sequences ; Phaseolus vulgaris ; Phosphotransferase ; Phosphotransferase II ; phosphotransferases (phosphomutases) ; Plant viruses ; Polymerase chain reaction ; rapid methods ; reverse transcriptase polymerase chain reaction ; Ribonuclease ; Ribonucleic acid ; RNA ; Solanum lycopersicum var. lycopersicum ; Tobacco ; Tomatoes ; Transgenes ; Transgenic plants ; Trifolium ; Triticum aestivum ; Viruses ; Vitis ; Wheat ; Wheat streak mosaic virus</subject><ispartof>Plant molecular biology reporter, 2001-12, Vol.19 (4), p.329-340</ispartof><rights>International Society for Plant Molecular Biology 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c281t-4d025a18833079cde4bb3c2e505e0382c7320287a00f466361979cfcc8f19b913</citedby><cites>FETCH-LOGICAL-c281t-4d025a18833079cde4bb3c2e505e0382c7320287a00f466361979cfcc8f19b913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Myslik, J.T</creatorcontrib><creatorcontrib>Nassuth, A</creatorcontrib><title>Rapid detection of viruses, transgenes, and mRNAs in small plant leaf samples</title><title>Plant molecular biology reporter</title><description>A simple method for the detection of DNA and RNA from small tissue samples (<-1 mg) is described. Stabs of fresh leaves from wheat, clover, tobacco, broad bean, grape, tomato, lettuce, or asparagus were taken using glass microcapillaries, combined with RNase inhibitor and subjected to RT-PCR either directly or after a DNase treatment. This method was used to successfully detect the presence of (1) virus in infected plants, (2) transgenes such asneomycin phosphotransferase II in transformed plants and (3) various plant genes including RubiscoL and 1,3-β-D-glucanase. With the described DNase treatment, the technique was shown to effectively detect RNA and, therefore, can be used to localize and study patterns of gene expression, virus distribution, and/or transgene expression. The benefits of the described tissue stab technique are that it is quick, it requires little manipulation, and is applicable to a variety of plants.</description><subject>Asparagus</subject><subject>Asparagus officinalis</subject><subject>Clover</subject><subject>Deoxyribonuclease</subject><subject>Deoxyribonucleic acid</subject><subject>detection</subject><subject>DNA</subject><subject>DNA fingerprinting</subject><subject>Gene expression</subject><subject>Lactuca sativa</subject><subject>Leaves</subject><subject>messenger RNA</subject><subject>molecular sequence data</subject><subject>neomycin</subject><subject>Nicotiana tabacum</subject><subject>nucleotide sequences</subject><subject>Phaseolus vulgaris</subject><subject>Phosphotransferase</subject><subject>Phosphotransferase II</subject><subject>phosphotransferases (phosphomutases)</subject><subject>Plant viruses</subject><subject>Polymerase chain reaction</subject><subject>rapid methods</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Ribonuclease</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Solanum lycopersicum var. lycopersicum</subject><subject>Tobacco</subject><subject>Tomatoes</subject><subject>Transgenes</subject><subject>Transgenic plants</subject><subject>Trifolium</subject><subject>Triticum aestivum</subject><subject>Viruses</subject><subject>Vitis</subject><subject>Wheat</subject><subject>Wheat streak mosaic virus</subject><issn>0735-9640</issn><issn>1572-9818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpF0M1KAzEUBeAgCtbqxhcw4E4cvUlmJsmyFqtCVah2HdJMUqbMn7kzgm_vlAquzll83AuHkEsGdwxA3j8sgEvJlWBHZMIyyROtmDomE5AiS3Sewik5Q9zBiEGpCXld2a4saOF77_qybWgb6HcZB_R4S_toG9z6Zt9tU9B69TZDWjYUa1tVtKts09PK20DR1l3l8ZycBFuhv_jLKVkvHj_nz8ny_ellPlsmjivWJ2kBPLNMKSFAalf4dLMRjvsMMg9CcScFB66kBQhpnouc6ZEF51RgeqOZmJLrw90utl-Dx97s2iE240vDdJbmUkngo7o5KBdbxOiD6WJZ2_hjGJj9XOZ_rhFfHXCwrbHbWKJZf3BgAkCnWoEQv-kfY2c</recordid><startdate>20011201</startdate><enddate>20011201</enddate><creator>Myslik, J.T</creator><creator>Nassuth, A</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20011201</creationdate><title>Rapid detection of viruses, transgenes, and mRNAs in small plant leaf samples</title><author>Myslik, J.T ; Nassuth, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c281t-4d025a18833079cde4bb3c2e505e0382c7320287a00f466361979cfcc8f19b913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Asparagus</topic><topic>Asparagus officinalis</topic><topic>Clover</topic><topic>Deoxyribonuclease</topic><topic>Deoxyribonucleic acid</topic><topic>detection</topic><topic>DNA</topic><topic>DNA fingerprinting</topic><topic>Gene expression</topic><topic>Lactuca sativa</topic><topic>Leaves</topic><topic>messenger RNA</topic><topic>molecular sequence data</topic><topic>neomycin</topic><topic>Nicotiana tabacum</topic><topic>nucleotide sequences</topic><topic>Phaseolus vulgaris</topic><topic>Phosphotransferase</topic><topic>Phosphotransferase II</topic><topic>phosphotransferases (phosphomutases)</topic><topic>Plant viruses</topic><topic>Polymerase chain reaction</topic><topic>rapid methods</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>Ribonuclease</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Solanum lycopersicum var. lycopersicum</topic><topic>Tobacco</topic><topic>Tomatoes</topic><topic>Transgenes</topic><topic>Transgenic plants</topic><topic>Trifolium</topic><topic>Triticum aestivum</topic><topic>Viruses</topic><topic>Vitis</topic><topic>Wheat</topic><topic>Wheat streak mosaic virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Myslik, J.T</creatorcontrib><creatorcontrib>Nassuth, A</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology reporter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Myslik, J.T</au><au>Nassuth, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of viruses, transgenes, and mRNAs in small plant leaf samples</atitle><jtitle>Plant molecular biology reporter</jtitle><date>2001-12-01</date><risdate>2001</risdate><volume>19</volume><issue>4</issue><spage>329</spage><epage>340</epage><pages>329-340</pages><issn>0735-9640</issn><eissn>1572-9818</eissn><abstract>A simple method for the detection of DNA and RNA from small tissue samples (<-1 mg) is described. Stabs of fresh leaves from wheat, clover, tobacco, broad bean, grape, tomato, lettuce, or asparagus were taken using glass microcapillaries, combined with RNase inhibitor and subjected to RT-PCR either directly or after a DNase treatment. This method was used to successfully detect the presence of (1) virus in infected plants, (2) transgenes such asneomycin phosphotransferase II in transformed plants and (3) various plant genes including RubiscoL and 1,3-β-D-glucanase. With the described DNase treatment, the technique was shown to effectively detect RNA and, therefore, can be used to localize and study patterns of gene expression, virus distribution, and/or transgene expression. The benefits of the described tissue stab technique are that it is quick, it requires little manipulation, and is applicable to a variety of plants.</abstract><cop>New York</cop><pub>Springer Nature B.V</pub><doi>10.1007/BF02772831</doi><tpages>12</tpages></addata></record> |
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| subjects | Asparagus Asparagus officinalis Clover Deoxyribonuclease Deoxyribonucleic acid detection DNA DNA fingerprinting Gene expression Lactuca sativa Leaves messenger RNA molecular sequence data neomycin Nicotiana tabacum nucleotide sequences Phaseolus vulgaris Phosphotransferase Phosphotransferase II phosphotransferases (phosphomutases) Plant viruses Polymerase chain reaction rapid methods reverse transcriptase polymerase chain reaction Ribonuclease Ribonucleic acid RNA Solanum lycopersicum var. lycopersicum Tobacco Tomatoes Transgenes Transgenic plants Trifolium Triticum aestivum Viruses Vitis Wheat Wheat streak mosaic virus |
| title | Rapid detection of viruses, transgenes, and mRNAs in small plant leaf samples |
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