An improved method for isolating RNA from dehydrated and nondehydrated chili pepper (Capsicum annuum L.) plant tissues
High-quality RNA is important in studying gene expression. This report describes an improved method for isolating intact purified RNA from dehydrated organs of chili pepper plants. Common RNA extraction protocols have produced poor yields because dehydrated leaves accumulate polysaccharides and RNas...
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| Veröffentlicht in: | Plant molecular biology reporter 2002-12, Vol.20 (4), p.407-414 |
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| creator | Camacho-Villasana, Yolanda M. Ochoa-Alejo, Neftali Walling, Linda Bray, Elizabeth A. |
| description | High-quality RNA is important in studying gene expression. This report describes an improved method for isolating intact purified RNA from dehydrated organs of chili pepper plants. Common RNA extraction protocols have produced poor yields because dehydrated leaves accumulate polysaccharides and RNases. Our protocol is based on a guanidine thiocyanate extraction combined with additional purification steps using butanol and the ionic detergent CTAB (cetyltrimethylammonium bromide). Using this protocol, RNA yields ranged from 40-70 μg of total RNA per 200 mg of fresh tissue. This method can be adapted to large-scale isolations, allowing the recovery of larger amounts of intact RNA (up to 250 μg per gram of fresh tissue). |
| doi_str_mv | 10.1007/BF02772128 |
| format | Article |
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This report describes an improved method for isolating intact purified RNA from dehydrated organs of chili pepper plants. Common RNA extraction protocols have produced poor yields because dehydrated leaves accumulate polysaccharides and RNases. Our protocol is based on a guanidine thiocyanate extraction combined with additional purification steps using butanol and the ionic detergent CTAB (cetyltrimethylammonium bromide). Using this protocol, RNA yields ranged from 40-70 μg of total RNA per 200 mg of fresh tissue. This method can be adapted to large-scale isolations, allowing the recovery of larger amounts of intact RNA (up to 250 μg per gram of fresh tissue).</abstract><cop>New York</cop><pub>Springer Nature B.V</pub><doi>10.1007/BF02772128</doi><tpages>8</tpages></addata></record> |
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| ispartof | Plant molecular biology reporter, 2002-12, Vol.20 (4), p.407-414 |
| issn | 0735-9640 1572-9818 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Butanol Capsicum annuum Cetyltrimethylammonium bromide Dehydration Gene expression Guanidine Organs Peppers Plant extracts Plant tissues Polysaccharides Purification Ribonucleic acid RNA Saccharides |
| title | An improved method for isolating RNA from dehydrated and nondehydrated chili pepper (Capsicum annuum L.) plant tissues |
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