Identification of small nuclear DNA molecules of the cuban sugarcane cultivar Ja60-5 via pulsed field gel electrophoresis
We present evidence of the existence of at least 7 small nuclear DNA molecules in the Ja60-5 sugarcane cultivar genome. These small molecules were separated by means of transversal alternating field electrophoresis (TAFE); their sizes ranged from about 230-3000 kbp, and they hybridized with telomeri...
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| Veröffentlicht in: | Plant molecular biology reporter 2005-03, Vol.23 (1), p.81-82 |
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| container_title | Plant molecular biology reporter |
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| creator | Crespo, Esperanza Niubó López-Canovas, Lilia Riverón, Ana María Canales, Eduardo Coto, Orlando Noa, Maria D. |
| description | We present evidence of the existence of at least 7 small nuclear DNA molecules in the Ja60-5 sugarcane cultivar genome. These small molecules were separated by means of transversal alternating field electrophoresis (TAFE); their sizes ranged from about 230-3000 kbp, and they hybridized with telomeric, subcentromeric, and various nuclear DNA probes. Here, we also report the use and the modifications of DNA from microorganisms, to immobilize nuclear DNA from sugarcane suitable for analysis via pulsed field gel electrophoresis. The protocol is simple, inexpensive, and easy to perform. |
| doi_str_mv | 10.1007/BF02772652 |
| format | Article |
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| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Cultivars Deoxyribonucleic acid DNA DNA probes Electrophoresis Food processing industry Gel electrophoresis Genomes Hybridization Microorganisms Sugarcane |
| title | Identification of small nuclear DNA molecules of the cuban sugarcane cultivar Ja60-5 via pulsed field gel electrophoresis |
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