An improved clearing method for GUS assay inArabidopsis endosperm and seeds

An improved clearing method for GUS assay inArabidopsis endosperm and seeds

Precise cellular localization of the GUS stain is notoriously difficult inArabidopsis seeds. Here we report an improved protocol for the clearing of seeds after GUS staining. Incubation in ethanol-acetic acid (EtAc) and Hoyer's medium allows reliable cellular localization of the GUS, even in se...

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Veröffentlicht in:Plant molecular biology reporter 2002-06, Vol.20 (2), p.107-114
Hauptverfasser: Stangeland, Biljana, Salehian, Zhian
Format: Artikel
Sprache:eng
Schlagworte:
Acetic acid
Clearing
Developmental stages
Endosperm
Ethanol
Localization
Nucleoli
Seeds
Staining
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Zusammenfassung:Precise cellular localization of the GUS stain is notoriously difficult inArabidopsis seeds. Here we report an improved protocol for the clearing of seeds after GUS staining. Incubation in ethanol-acetic acid (EtAc) and Hoyer's medium allows reliable cellular localization of the GUS, even in seeds from late developmental stages. This method also leads to the staining of nucleoli in the endosperm and embryo, facilitating nuclear counts in endosperm development.
ISSN:0735-9640
1572-9818
DOI:10.1007/BF02799426