Flow sorting of wheat chromosome arms from the ditelosomic line 7BL

Flow cytometric analysis confirmed that root tip cells can be synchronized with 1.25 mM hydroxyurea (DNA synthesis inhibitor) for 12 h and 1 μM trifluralin (metaphase blocking reagent) treatment for 5 h. Chromosome suspensions prepared from homogenized tissue were suitable for chromosome sorting. A...

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Veröffentlicht in:	Plant molecular biology reporter 2006-03, Vol.24 (1), p.23-31
Hauptverfasser:	Guo, D.W, Ma, Y.Z, Li, L.C, Chen, Y.F
Format:	Artikel
Sprache:	eng
Schlagworte:	chromosome mapping Chromosomes Deoxyribonucleic acid DNA DNA biosynthesis Flow cytometry genes Genomes Hydroxyurea karyotyping Metaphase Sample preparation Trifluralin Triticum aestivum Wheat
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