Flow sorting of wheat chromosome arms from the ditelosomic line 7BL
Flow cytometric analysis confirmed that root tip cells can be synchronized with 1.25 mM hydroxyurea (DNA synthesis inhibitor) for 12 h and 1 μM trifluralin (metaphase blocking reagent) treatment for 5 h. Chromosome suspensions prepared from homogenized tissue were suitable for chromosome sorting. A...
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| Veröffentlicht in: | Plant molecular biology reporter 2006-03, Vol.24 (1), p.23-31 |
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| creator | Guo, D.W Ma, Y.Z Li, L.C Chen, Y.F |
| description | Flow cytometric analysis confirmed that root tip cells can be synchronized with 1.25 mM hydroxyurea (DNA synthesis inhibitor) for 12 h and 1 μM trifluralin (metaphase blocking reagent) treatment for 5 h. Chromosome suspensions prepared from homogenized tissue were suitable for chromosome sorting. A flow karyotypic histogram showed that the genome of common wheat (Triticum aestivum L.) 'Chinese Spring' was divided into 4 chromosome peaks, but the 7BL ditelosomic line had an additional chromosome peak. PCR amplification of sorted chromosome arms indicated that the extra chromosome peak consisted of 7BL telosomics. Some technical details of sample preparation and parameter setting for flow cytometric analysis are described. |
| doi_str_mv | 10.1007/BF02914043 |
| format | Article |
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| ispartof | Plant molecular biology reporter, 2006-03, Vol.24 (1), p.23-31 |
| issn | 0735-9640 1572-9818 |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | chromosome mapping Chromosomes Deoxyribonucleic acid DNA DNA biosynthesis Flow cytometry genes Genomes Hydroxyurea karyotyping Metaphase Sample preparation Trifluralin Triticum aestivum Wheat |
| title | Flow sorting of wheat chromosome arms from the ditelosomic line 7BL |
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