Efficacy of Vascular Endothelial Growth Factor in the Treatment of Experimental Gastric Injury
Background/Aims: Studies indicate that angiogenesis is important in tissue healing. However, the role of vascular endothelial growth factor (VEGF) in tissue healing has not been established. The aim of the study is to determine whether VEGF has a gastroprotective role in experimental gastric injury....
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| Veröffentlicht in: | Digestion 2002-01, Vol.66 (2), p.99-105 |
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| creator | Matsui, Yutaka Mitsuyama, Keiichi Tomiyasu, Nobuo Toyonaga, Atsushi Sata, Michio |
| description | Background/Aims: Studies indicate that angiogenesis is important in tissue healing. However, the role of vascular endothelial growth factor (VEGF) in tissue healing has not been established. The aim of the study is to determine whether VEGF has a gastroprotective role in experimental gastric injury. Methods: Acute gastric injury was induced in Sprague-Dawley rats by intragastric administration of 100% ethanol. Expression of gastric VEGF was determined in tissue homogenates by enzyme-linked immunosorbent assay and in paraffin-embedded sections by immunohistochemistry. The effect of systemic administration of anti-VEGF antibodies and recombinant VEGF on injury severity was assessed macroscopically and microscopically. Results: Gastric VEGF concentrations peaked at 6 h and again 3 days after acute injury. The presence of VEGF was demonstrated in epithelial cells and in mononuclear cells. Blocking endogenous VEGF effects with anti-VEGF antibodies exacerbated mucosal injury. Administration of recombinant VEGF after the onset of injury reduced the severity of mucosal injury, irrespective of the timing of initial treatment with VEGF. Immunohistochemical detection of vascular endothelial cells revealed that the VEGF-induced mucosal repair is closely related to the degree of angiogenesis. Conclusion: The results provide strong evidence for the role of VEGF in the repair of tissue damage induced by ethanol. The results also show how VEGF may be used in a clinical setting to treat some acute gastric lesions. |
| doi_str_mv | 10.1159/000065591 |
| format | Article |
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However, the role of vascular endothelial growth factor (VEGF) in tissue healing has not been established. The aim of the study is to determine whether VEGF has a gastroprotective role in experimental gastric injury. Methods: Acute gastric injury was induced in Sprague-Dawley rats by intragastric administration of 100% ethanol. Expression of gastric VEGF was determined in tissue homogenates by enzyme-linked immunosorbent assay and in paraffin-embedded sections by immunohistochemistry. The effect of systemic administration of anti-VEGF antibodies and recombinant VEGF on injury severity was assessed macroscopically and microscopically. Results: Gastric VEGF concentrations peaked at 6 h and again 3 days after acute injury. The presence of VEGF was demonstrated in epithelial cells and in mononuclear cells. Blocking endogenous VEGF effects with anti-VEGF antibodies exacerbated mucosal injury. Administration of recombinant VEGF after the onset of injury reduced the severity of mucosal injury, irrespective of the timing of initial treatment with VEGF. Immunohistochemical detection of vascular endothelial cells revealed that the VEGF-induced mucosal repair is closely related to the degree of angiogenesis. Conclusion: The results provide strong evidence for the role of VEGF in the repair of tissue damage induced by ethanol. The results also show how VEGF may be used in a clinical setting to treat some acute gastric lesions.</description><identifier>ISSN: 0012-2823</identifier><identifier>EISSN: 1421-9867</identifier><identifier>DOI: 10.1159/000065591</identifier><identifier>PMID: 12428069</identifier><identifier>CODEN: DIGEBW</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Animals ; Antibodies - pharmacology ; Biological and medical sciences ; Digestive system ; Endothelial Growth Factors - immunology ; Endothelial Growth Factors - metabolism ; Endothelial Growth Factors - therapeutic use ; Ethanol ; Female ; Gastric Mucosa - drug effects ; Gastric Mucosa - metabolism ; Immunohistochemistry ; Intercellular Signaling Peptides and Proteins - immunology ; Intercellular Signaling Peptides and Proteins - metabolism ; Intercellular Signaling Peptides and Proteins - therapeutic use ; Investigative techniques, diagnostic techniques (general aspects) ; Lymphokines - immunology ; Lymphokines - metabolism ; Lymphokines - therapeutic use ; Medical sciences ; Neovascularization, Physiologic ; Original Paper: Gastric Disorders ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Rats ; Rats, Sprague-Dawley ; Stomach Diseases - chemically induced ; Stomach Diseases - drug therapy ; Stomach Diseases - metabolism ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors</subject><ispartof>Digestion, 2002-01, Vol.66 (2), p.99-105</ispartof><rights>2002 S. Karger AG, Basel</rights><rights>2003 INIST-CNRS</rights><rights>Copyright 2002 S. Karger AG, Basel</rights><rights>Copyright S. Karger AG 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-4c0f965d918e1b2d983810fc752814d6282d4fa8b449ea9790958fa05f6448533</citedby><cites>FETCH-LOGICAL-c451t-4c0f965d918e1b2d983810fc752814d6282d4fa8b449ea9790958fa05f6448533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2423,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14372520$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12428069$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsui, Yutaka</creatorcontrib><creatorcontrib>Mitsuyama, Keiichi</creatorcontrib><creatorcontrib>Tomiyasu, Nobuo</creatorcontrib><creatorcontrib>Toyonaga, Atsushi</creatorcontrib><creatorcontrib>Sata, Michio</creatorcontrib><title>Efficacy of Vascular Endothelial Growth Factor in the Treatment of Experimental Gastric Injury</title><title>Digestion</title><addtitle>Digestion</addtitle><description>Background/Aims: Studies indicate that angiogenesis is important in tissue healing. However, the role of vascular endothelial growth factor (VEGF) in tissue healing has not been established. The aim of the study is to determine whether VEGF has a gastroprotective role in experimental gastric injury. Methods: Acute gastric injury was induced in Sprague-Dawley rats by intragastric administration of 100% ethanol. Expression of gastric VEGF was determined in tissue homogenates by enzyme-linked immunosorbent assay and in paraffin-embedded sections by immunohistochemistry. The effect of systemic administration of anti-VEGF antibodies and recombinant VEGF on injury severity was assessed macroscopically and microscopically. Results: Gastric VEGF concentrations peaked at 6 h and again 3 days after acute injury. The presence of VEGF was demonstrated in epithelial cells and in mononuclear cells. Blocking endogenous VEGF effects with anti-VEGF antibodies exacerbated mucosal injury. Administration of recombinant VEGF after the onset of injury reduced the severity of mucosal injury, irrespective of the timing of initial treatment with VEGF. Immunohistochemical detection of vascular endothelial cells revealed that the VEGF-induced mucosal repair is closely related to the degree of angiogenesis. Conclusion: The results provide strong evidence for the role of VEGF in the repair of tissue damage induced by ethanol. The results also show how VEGF may be used in a clinical setting to treat some acute gastric lesions.</description><subject>Animals</subject><subject>Antibodies - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Digestive system</subject><subject>Endothelial Growth Factors - immunology</subject><subject>Endothelial Growth Factors - metabolism</subject><subject>Endothelial Growth Factors - therapeutic use</subject><subject>Ethanol</subject><subject>Female</subject><subject>Gastric Mucosa - drug effects</subject><subject>Gastric Mucosa - metabolism</subject><subject>Immunohistochemistry</subject><subject>Intercellular Signaling Peptides and Proteins - immunology</subject><subject>Intercellular Signaling Peptides and Proteins - metabolism</subject><subject>Intercellular Signaling Peptides and Proteins - therapeutic use</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lymphokines - immunology</subject><subject>Lymphokines - metabolism</subject><subject>Lymphokines - therapeutic use</subject><subject>Medical sciences</subject><subject>Neovascularization, Physiologic</subject><subject>Original Paper: Gastric Disorders</subject><subject>Pathology. 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Miscellaneous investigative techniques</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Stomach Diseases - chemically induced</subject><subject>Stomach Diseases - drug therapy</subject><subject>Stomach Diseases - metabolism</subject><subject>Vascular Endothelial Growth Factor A</subject><subject>Vascular Endothelial Growth Factors</subject><issn>0012-2823</issn><issn>1421-9867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpt0EFPwyAUB3BiNG5OD55NDFniwUMVKLRwNLObS5Z4mR5tGAXX2bUT2ui-vdTV7SIX8vJ-8OAPwCVGdxgzcY_8ihgT-Aj0MSU4EDyKj0EfIUwCwknYA2fOrdpS0PAU9DChhKNI9MFbYkyupNrCysBX6VRTSAuTMqvqpS5yWcCJrb7qJRxLVVcW5iX0DTi3WtZrXdbtseR7o23eVi2Xrra5gtNy1djtOTgxsnD6otsH4GWczEdPwex5Mh09zAJFGa4DqpAREcsE5hovSCZ4yDEyKmaEY5pF_g8ZNZIvKBVailggwbiRiJmIUs7CcACGu3s3tvpstKvTVdXY0o9MsWBYhJzGHt3ukLKVc1abdOOfLe02xShtg0z3QXp73V3YLNY6O8guOQ9uOuBDk4WxslS5OzgaxoQR5N3Vzn1I-67tHvyNGf7bfZxOfkG6yUz4A2iwjTw</recordid><startdate>20020101</startdate><enddate>20020101</enddate><creator>Matsui, Yutaka</creator><creator>Mitsuyama, Keiichi</creator><creator>Tomiyasu, Nobuo</creator><creator>Toyonaga, Atsushi</creator><creator>Sata, Michio</creator><general>Karger</general><general>S. 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Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Stomach Diseases - chemically induced</topic><topic>Stomach Diseases - drug therapy</topic><topic>Stomach Diseases - metabolism</topic><topic>Vascular Endothelial Growth Factor A</topic><topic>Vascular Endothelial Growth Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsui, Yutaka</creatorcontrib><creatorcontrib>Mitsuyama, Keiichi</creatorcontrib><creatorcontrib>Tomiyasu, Nobuo</creatorcontrib><creatorcontrib>Toyonaga, Atsushi</creatorcontrib><creatorcontrib>Sata, Michio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>SIRS Editorial</collection><jtitle>Digestion</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsui, Yutaka</au><au>Mitsuyama, Keiichi</au><au>Tomiyasu, Nobuo</au><au>Toyonaga, Atsushi</au><au>Sata, Michio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficacy of Vascular Endothelial Growth Factor in the Treatment of Experimental Gastric Injury</atitle><jtitle>Digestion</jtitle><addtitle>Digestion</addtitle><date>2002-01-01</date><risdate>2002</risdate><volume>66</volume><issue>2</issue><spage>99</spage><epage>105</epage><pages>99-105</pages><issn>0012-2823</issn><eissn>1421-9867</eissn><coden>DIGEBW</coden><abstract>Background/Aims: Studies indicate that angiogenesis is important in tissue healing. However, the role of vascular endothelial growth factor (VEGF) in tissue healing has not been established. The aim of the study is to determine whether VEGF has a gastroprotective role in experimental gastric injury. Methods: Acute gastric injury was induced in Sprague-Dawley rats by intragastric administration of 100% ethanol. Expression of gastric VEGF was determined in tissue homogenates by enzyme-linked immunosorbent assay and in paraffin-embedded sections by immunohistochemistry. The effect of systemic administration of anti-VEGF antibodies and recombinant VEGF on injury severity was assessed macroscopically and microscopically. Results: Gastric VEGF concentrations peaked at 6 h and again 3 days after acute injury. The presence of VEGF was demonstrated in epithelial cells and in mononuclear cells. Blocking endogenous VEGF effects with anti-VEGF antibodies exacerbated mucosal injury. Administration of recombinant VEGF after the onset of injury reduced the severity of mucosal injury, irrespective of the timing of initial treatment with VEGF. Immunohistochemical detection of vascular endothelial cells revealed that the VEGF-induced mucosal repair is closely related to the degree of angiogenesis. Conclusion: The results provide strong evidence for the role of VEGF in the repair of tissue damage induced by ethanol. The results also show how VEGF may be used in a clinical setting to treat some acute gastric lesions.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>12428069</pmid><doi>10.1159/000065591</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Antibodies - pharmacology Biological and medical sciences Digestive system Endothelial Growth Factors - immunology Endothelial Growth Factors - metabolism Endothelial Growth Factors - therapeutic use Ethanol Female Gastric Mucosa - drug effects Gastric Mucosa - metabolism Immunohistochemistry Intercellular Signaling Peptides and Proteins - immunology Intercellular Signaling Peptides and Proteins - metabolism Intercellular Signaling Peptides and Proteins - therapeutic use Investigative techniques, diagnostic techniques (general aspects) Lymphokines - immunology Lymphokines - metabolism Lymphokines - therapeutic use Medical sciences Neovascularization, Physiologic Original Paper: Gastric Disorders Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Rats Rats, Sprague-Dawley Stomach Diseases - chemically induced Stomach Diseases - drug therapy Stomach Diseases - metabolism Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| title | Efficacy of Vascular Endothelial Growth Factor in the Treatment of Experimental Gastric Injury |
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