Nuclear DNA Content as an Index Character Discriminating Taxa in the Genus Petunia sensu Jussieu (Solanaceae)
Nuclear DNA contents over the total range of the genus Petunia sensu Jussieu comprising 20 taxa of Petunia sensu Wijsman (2 n=2 x=14) and 32 taxa of Calibrachoa(2 n=2 x=18) were estimated by flow cytometry after staining the nuclei with propidium iodide (PI) or 4′,6-diamidino-2-phenylindole (DAPI)....
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| Veröffentlicht in: | Annals of botany 2000-05, Vol.85 (5), p.665-673 |
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| container_title | Annals of botany |
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| creator | Mishiba, Kei-Ichiro Ando, Toshio Mii, Masahiro Watanabe, Hitoshi Kokubun, Hisashi Hashimoto, Goro Marchesi, Eduardo |
| description | Nuclear DNA contents over the total range of the genus Petunia sensu Jussieu comprising 20 taxa of Petunia sensu Wijsman (2 n=2 x=14) and 32 taxa of Calibrachoa(2 n=2 x=18) were estimated by flow cytometry after staining the nuclei with propidium iodide (PI) or 4′,6-diamidino-2-phenylindole (DAPI). With respect to nuclear DNA content, taxa of Petunia sensu Wijsman seemed to be homogeneous (2C=2.60 to 3.41pg), but Calibrachoa taxa were clearly separated into two groups: (1)C. parviflora and C. pygmaea(1.56 to 1.91pg); and (2) remaining members of Calibrachoa(2.84 to 3.26pg). Taxa of Petunia sensu Wijsman exhibited larger PI/DAPI ratios (relative fluorescence intensity with PI staining to that with DAPI staining) than Calibrachoa species exceptC. parviflora and C. pygmaea. This suggests that Petunia sensu Wijsman has nuclear DNA with more adenine-thymine rich regions than Calibrachoa. |
| doi_str_mv | 10.1006/anbo.2000.1122 |
| format | Article |
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With respect to nuclear DNA content, taxa of Petunia sensu Wijsman seemed to be homogeneous (2C=2.60 to 3.41pg), but Calibrachoa taxa were clearly separated into two groups: (1)C. parviflora and C. pygmaea(1.56 to 1.91pg); and (2) remaining members of Calibrachoa(2.84 to 3.26pg). Taxa of Petunia sensu Wijsman exhibited larger PI/DAPI ratios (relative fluorescence intensity with PI staining to that with DAPI staining) than Calibrachoa species exceptC. parviflora and C. pygmaea. This suggests that Petunia sensu Wijsman has nuclear DNA with more adenine-thymine rich regions than Calibrachoa.</description><identifier>ISSN: 0305-7364</identifier><identifier>EISSN: 1095-8290</identifier><identifier>DOI: 10.1006/anbo.2000.1122</identifier><language>eng</language><publisher>Oxford: Elsevier Science Ltd</publisher><subject>6-diamidino-2-phenylindole (DAPI) ; Biological taxonomies ; Calibrachoa ; Calibrachoa, 4′,6-diamidino-2-phenylindole (DAPI), flow cytometry, nuclear DNA content,Petunia , PI/DAPI ratio, propidium iodide (PI), Solanaceae ; Chromosomes ; DNA ; Estimated taxes ; flow cytometry ; Fluorescence ; Horticulture ; Hybridity ; nuclear DNA content ; Petunia ; PI/DAPI ratio ; Plants ; Pollen ; propidium iodide (PI) ; Solanaceae ; Taxa</subject><ispartof>Annals of botany, 2000-05, Vol.85 (5), p.665-673</ispartof><rights>2000 Annals of Botany Company</rights><rights>Copyright Oxford University Press(England) May 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-572844d8922dfd2d90681fc9607c1818cdf433f95bc89c2bcc27d5af5c490e573</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42770740$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42770740$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids></links><search><creatorcontrib>Mishiba, Kei-Ichiro</creatorcontrib><creatorcontrib>Ando, Toshio</creatorcontrib><creatorcontrib>Mii, Masahiro</creatorcontrib><creatorcontrib>Watanabe, Hitoshi</creatorcontrib><creatorcontrib>Kokubun, Hisashi</creatorcontrib><creatorcontrib>Hashimoto, Goro</creatorcontrib><creatorcontrib>Marchesi, Eduardo</creatorcontrib><title>Nuclear DNA Content as an Index Character Discriminating Taxa in the Genus Petunia sensu Jussieu (Solanaceae)</title><title>Annals of botany</title><addtitle>Ann Bot</addtitle><description>Nuclear DNA contents over the total range of the genus Petunia sensu Jussieu comprising 20 taxa of Petunia sensu Wijsman (2 n=2 x=14) and 32 taxa of Calibrachoa(2 n=2 x=18) were estimated by flow cytometry after staining the nuclei with propidium iodide (PI) or 4′,6-diamidino-2-phenylindole (DAPI). With respect to nuclear DNA content, taxa of Petunia sensu Wijsman seemed to be homogeneous (2C=2.60 to 3.41pg), but Calibrachoa taxa were clearly separated into two groups: (1)C. parviflora and C. pygmaea(1.56 to 1.91pg); and (2) remaining members of Calibrachoa(2.84 to 3.26pg). Taxa of Petunia sensu Wijsman exhibited larger PI/DAPI ratios (relative fluorescence intensity with PI staining to that with DAPI staining) than Calibrachoa species exceptC. parviflora and C. pygmaea. This suggests that Petunia sensu Wijsman has nuclear DNA with more adenine-thymine rich regions than Calibrachoa.</description><subject>6-diamidino-2-phenylindole (DAPI)</subject><subject>Biological taxonomies</subject><subject>Calibrachoa</subject><subject>Calibrachoa, 4′,6-diamidino-2-phenylindole (DAPI), flow cytometry, nuclear DNA content,Petunia , PI/DAPI ratio, propidium iodide (PI), Solanaceae</subject><subject>Chromosomes</subject><subject>DNA</subject><subject>Estimated taxes</subject><subject>flow cytometry</subject><subject>Fluorescence</subject><subject>Horticulture</subject><subject>Hybridity</subject><subject>nuclear DNA content</subject><subject>Petunia</subject><subject>PI/DAPI ratio</subject><subject>Plants</subject><subject>Pollen</subject><subject>propidium iodide (PI)</subject><subject>Solanaceae</subject><subject>Taxa</subject><issn>0305-7364</issn><issn>1095-8290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNp1kE1vEzEQQC0EEqH0yq2S1RM9bLC967V9rFKaFKJQRJGqXizHnm0dUm-xvSj8e7zaqjdOo9G8-XoIfaBkTglpP5mw7eeMkJJSxl6hGSWKV5Ip8hrNSE14Jeq2eYvepbQrFGsVnaHHzWD3YCK-2JzjRR8yhIxNwibgq-DggBcPJhqboRA-2egffTDZh3t8Yw4G-4DzA-AlhCHha8hD8AYnCGnAX4aUPAz4449-b4KxYODsPXrTmX2C4-d4hH5efr5ZrKr1t-XV4nxd2UaoXHHBZNM4qRhznWNOkVbSzqqWCEslldZ1TV13im-tVJZtrWXCcdNx2ygCXNRH6HSa-xT73wOkrHf9EENZqanihLeFK9B8gmzsU4rQ6afynol_NSV6NKpHo3o0qkejpeFkatil3McXumFCENGMA6up7lOGw0vdxF-6FbXgenV7p9lKXmzW37_qZeHlxENR8cdD1Ml6CBacj2Czdr3_3yn_ACbvlLs</recordid><startdate>20000501</startdate><enddate>20000501</enddate><creator>Mishiba, Kei-Ichiro</creator><creator>Ando, Toshio</creator><creator>Mii, Masahiro</creator><creator>Watanabe, Hitoshi</creator><creator>Kokubun, Hisashi</creator><creator>Hashimoto, Goro</creator><creator>Marchesi, Eduardo</creator><general>Elsevier Science Ltd</general><general>Oxford University Press</general><general>Academic Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20000501</creationdate><title>Nuclear DNA Content as an Index Character Discriminating Taxa in the Genus Petunia sensu Jussieu (Solanaceae)</title><author>Mishiba, Kei-Ichiro ; Ando, Toshio ; Mii, Masahiro ; Watanabe, Hitoshi ; Kokubun, Hisashi ; Hashimoto, Goro ; Marchesi, Eduardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-572844d8922dfd2d90681fc9607c1818cdf433f95bc89c2bcc27d5af5c490e573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>6-diamidino-2-phenylindole (DAPI)</topic><topic>Biological taxonomies</topic><topic>Calibrachoa</topic><topic>Calibrachoa, 4′,6-diamidino-2-phenylindole (DAPI), flow cytometry, nuclear DNA content,Petunia , PI/DAPI ratio, propidium iodide (PI), Solanaceae</topic><topic>Chromosomes</topic><topic>DNA</topic><topic>Estimated taxes</topic><topic>flow cytometry</topic><topic>Fluorescence</topic><topic>Horticulture</topic><topic>Hybridity</topic><topic>nuclear DNA content</topic><topic>Petunia</topic><topic>PI/DAPI ratio</topic><topic>Plants</topic><topic>Pollen</topic><topic>propidium iodide (PI)</topic><topic>Solanaceae</topic><topic>Taxa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mishiba, Kei-Ichiro</creatorcontrib><creatorcontrib>Ando, Toshio</creatorcontrib><creatorcontrib>Mii, Masahiro</creatorcontrib><creatorcontrib>Watanabe, Hitoshi</creatorcontrib><creatorcontrib>Kokubun, Hisashi</creatorcontrib><creatorcontrib>Hashimoto, Goro</creatorcontrib><creatorcontrib>Marchesi, Eduardo</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Annals of botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mishiba, Kei-Ichiro</au><au>Ando, Toshio</au><au>Mii, Masahiro</au><au>Watanabe, Hitoshi</au><au>Kokubun, Hisashi</au><au>Hashimoto, Goro</au><au>Marchesi, Eduardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear DNA Content as an Index Character Discriminating Taxa in the Genus Petunia sensu Jussieu (Solanaceae)</atitle><jtitle>Annals of botany</jtitle><addtitle>Ann Bot</addtitle><date>2000-05-01</date><risdate>2000</risdate><volume>85</volume><issue>5</issue><spage>665</spage><epage>673</epage><pages>665-673</pages><issn>0305-7364</issn><eissn>1095-8290</eissn><abstract>Nuclear DNA contents over the total range of the genus Petunia sensu Jussieu comprising 20 taxa of Petunia sensu Wijsman (2 n=2 x=14) and 32 taxa of Calibrachoa(2 n=2 x=18) were estimated by flow cytometry after staining the nuclei with propidium iodide (PI) or 4′,6-diamidino-2-phenylindole (DAPI). With respect to nuclear DNA content, taxa of Petunia sensu Wijsman seemed to be homogeneous (2C=2.60 to 3.41pg), but Calibrachoa taxa were clearly separated into two groups: (1)C. parviflora and C. pygmaea(1.56 to 1.91pg); and (2) remaining members of Calibrachoa(2.84 to 3.26pg). Taxa of Petunia sensu Wijsman exhibited larger PI/DAPI ratios (relative fluorescence intensity with PI staining to that with DAPI staining) than Calibrachoa species exceptC. parviflora and C. pygmaea. This suggests that Petunia sensu Wijsman has nuclear DNA with more adenine-thymine rich regions than Calibrachoa.</abstract><cop>Oxford</cop><pub>Elsevier Science Ltd</pub><doi>10.1006/anbo.2000.1122</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 6-diamidino-2-phenylindole (DAPI) Biological taxonomies Calibrachoa Calibrachoa, 4′,6-diamidino-2-phenylindole (DAPI), flow cytometry, nuclear DNA content,Petunia , PI/DAPI ratio, propidium iodide (PI), Solanaceae Chromosomes DNA Estimated taxes flow cytometry Fluorescence Horticulture Hybridity nuclear DNA content Petunia PI/DAPI ratio Plants Pollen propidium iodide (PI) Solanaceae Taxa |
| title | Nuclear DNA Content as an Index Character Discriminating Taxa in the Genus Petunia sensu Jussieu (Solanaceae) |
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