The non-classical MHC class I molecule Qa-1b inhibits classical MHC class I-restricted cytotoxicity of cytotoxic T lymphocytes
The CD94/NKG2A heterodimer is an inhibitory receptor expressed on a subset of mouse NK cells. CD94/NKG2A recognizes the non-classical MHC class I (class Ib) molecule Qa-1b and inhibits NK cytotoxicity. Qa-1b presents a peptide derived from the leader sequence of classical MHC class I molecules. Here...
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| Veröffentlicht in: | International immunology 2001-03, Vol.13 (3), p.321-327 |
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| creator | Lohwasser, Stefan Kubota, Akira Salcedo, Margarita Lian, Rebecca H. Takei1, Fumio |
| description | The CD94/NKG2A heterodimer is an inhibitory receptor expressed on a subset of mouse NK cells. CD94/NKG2A recognizes the non-classical MHC class I (class Ib) molecule Qa-1b and inhibits NK cytotoxicity. Qa-1b presents a peptide derived from the leader sequence of classical MHC class I molecules. Here, we examined the role of CD94/NKG2A in T cell-mediated cytotoxicity. Soluble tetrameric Qa-1b bound to almost all CD8+, but not CD4+, T cells. This binding seems to be mediated by CD8, because COS cells transfected with CD8 also bound Qa-1b tetramer. Therefore, the expression of CD94/NKG2 in T cells was further examined by single-cell RT-PCR. Most murine CD8+ T cells constitutively expressed CD94 and NKG2A transcripts, whereas they were not detected in CD4+ T cells. Co-expression of Qa-1b and Dk on target cells significantly inhibited cytotoxicity of Dk-specific cytotoxic T lymphocytes generated by mixed lymphocyte reaction, indicating that Qa-1b on antigen-presenting cells interacts with CD94/NKG2A on CD8 T cells and regulates classical MHC class I-restricted cytotoxic T cells. These results suggest a significant role of CD94/NKG2A as an inhibitory receptor on CD8+ T cells. |
| doi_str_mv | 10.1093/intimm/13.3.321 |
| format | Article |
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CD94/NKG2A recognizes the non-classical MHC class I (class Ib) molecule Qa-1b and inhibits NK cytotoxicity. Qa-1b presents a peptide derived from the leader sequence of classical MHC class I molecules. Here, we examined the role of CD94/NKG2A in T cell-mediated cytotoxicity. Soluble tetrameric Qa-1b bound to almost all CD8+, but not CD4+, T cells. This binding seems to be mediated by CD8, because COS cells transfected with CD8 also bound Qa-1b tetramer. Therefore, the expression of CD94/NKG2 in T cells was further examined by single-cell RT-PCR. Most murine CD8+ T cells constitutively expressed CD94 and NKG2A transcripts, whereas they were not detected in CD4+ T cells. Co-expression of Qa-1b and Dk on target cells significantly inhibited cytotoxicity of Dk-specific cytotoxic T lymphocytes generated by mixed lymphocyte reaction, indicating that Qa-1b on antigen-presenting cells interacts with CD94/NKG2A on CD8 T cells and regulates classical MHC class I-restricted cytotoxic T cells. These results suggest a significant role of CD94/NKG2A as an inhibitory receptor on CD8+ T cells.</description><identifier>ISSN: 0953-8178</identifier><identifier>EISSN: 1460-2377</identifier><identifier>DOI: 10.1093/intimm/13.3.321</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>APC allophycocyanin ; cell-cell interactions ; CTL cytotoxic T lymphocyte ; cytotoxic T lymphocyte ; GFP green fluorescence protein ; ITIM immunoreceptor tyrosine-based inhibitory motif ; KIR killer cell-inhibitory receptors ; MHC rodent ; MLR mixed lymphocyte reaction ; PE phycoerythrin ; SAvRED streptavidin–Red670 ; TGF transforming growth factor</subject><ispartof>International immunology, 2001-03, Vol.13 (3), p.321-327</ispartof><rights>Copyright Oxford University Press(England) Mar 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1510-a56d41b65d7a2447e99dc9d297efc4a46b0dad8d533d61385d6eb0dd0aa7f02c3</citedby><cites>FETCH-LOGICAL-c1510-a56d41b65d7a2447e99dc9d297efc4a46b0dad8d533d61385d6eb0dd0aa7f02c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids></links><search><creatorcontrib>Lohwasser, Stefan</creatorcontrib><creatorcontrib>Kubota, Akira</creatorcontrib><creatorcontrib>Salcedo, Margarita</creatorcontrib><creatorcontrib>Lian, Rebecca H.</creatorcontrib><creatorcontrib>Takei1, Fumio</creatorcontrib><title>The non-classical MHC class I molecule Qa-1b inhibits classical MHC class I-restricted cytotoxicity of cytotoxic T lymphocytes</title><title>International immunology</title><addtitle>Int. Immunol</addtitle><description>The CD94/NKG2A heterodimer is an inhibitory receptor expressed on a subset of mouse NK cells. CD94/NKG2A recognizes the non-classical MHC class I (class Ib) molecule Qa-1b and inhibits NK cytotoxicity. Qa-1b presents a peptide derived from the leader sequence of classical MHC class I molecules. Here, we examined the role of CD94/NKG2A in T cell-mediated cytotoxicity. Soluble tetrameric Qa-1b bound to almost all CD8+, but not CD4+, T cells. This binding seems to be mediated by CD8, because COS cells transfected with CD8 also bound Qa-1b tetramer. Therefore, the expression of CD94/NKG2 in T cells was further examined by single-cell RT-PCR. Most murine CD8+ T cells constitutively expressed CD94 and NKG2A transcripts, whereas they were not detected in CD4+ T cells. Co-expression of Qa-1b and Dk on target cells significantly inhibited cytotoxicity of Dk-specific cytotoxic T lymphocytes generated by mixed lymphocyte reaction, indicating that Qa-1b on antigen-presenting cells interacts with CD94/NKG2A on CD8 T cells and regulates classical MHC class I-restricted cytotoxic T cells. These results suggest a significant role of CD94/NKG2A as an inhibitory receptor on CD8+ T cells.</description><subject>APC allophycocyanin</subject><subject>cell-cell interactions</subject><subject>CTL cytotoxic T lymphocyte</subject><subject>cytotoxic T lymphocyte</subject><subject>GFP green fluorescence protein</subject><subject>ITIM immunoreceptor tyrosine-based inhibitory motif</subject><subject>KIR killer cell-inhibitory receptors</subject><subject>MHC rodent</subject><subject>MLR mixed lymphocyte reaction</subject><subject>PE phycoerythrin</subject><subject>SAvRED streptavidin–Red670</subject><subject>TGF transforming growth factor</subject><issn>0953-8178</issn><issn>1460-2377</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNptUEFLwzAYDaLgnJ69Bu9xSdM27VGGs9MNmUwQLyFNUpbZNjPJYL34261W9CLv8PEe733fxwPgkuBrgnM6MW0wTTMh9LpHRI7AiMQpRhFl7BiMcJ5QlBGWnYIz77cYYxrldAQ-1hsNW9siWQvvjRQ1XBZT-M3gHDa21nJfa7gSiJTQtBtTmuDhv27ktA_OyKAVlF2wwR6MNKGDtvrjcA3rrtltbK9ofw5OKlF7ffEzx-B5drueFmjxeDef3iyQJAnBSCSpikmZJoqJKI6ZznMlcxXlTFcyFnFaYiVUphJKVUpolqhU95LCQrAKR5KOwdWwd-fs-75_k2_t3rX9SU7yBFMaxbg3TQaTdNZ7pyu-c6YRruME86-O-dAxJ5T3iEifQEPC-KAPv3bh3njKKEt48fLKi9nT8mF1v-CUfgLOx4Gv</recordid><startdate>200103</startdate><enddate>200103</enddate><creator>Lohwasser, Stefan</creator><creator>Kubota, Akira</creator><creator>Salcedo, Margarita</creator><creator>Lian, Rebecca H.</creator><creator>Takei1, Fumio</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>200103</creationdate><title>The non-classical MHC class I molecule Qa-1b inhibits classical MHC class I-restricted cytotoxicity of cytotoxic T lymphocytes</title><author>Lohwasser, Stefan ; Kubota, Akira ; Salcedo, Margarita ; Lian, Rebecca H. ; Takei1, Fumio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1510-a56d41b65d7a2447e99dc9d297efc4a46b0dad8d533d61385d6eb0dd0aa7f02c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>APC allophycocyanin</topic><topic>cell-cell interactions</topic><topic>CTL cytotoxic T lymphocyte</topic><topic>cytotoxic T lymphocyte</topic><topic>GFP green fluorescence protein</topic><topic>ITIM immunoreceptor tyrosine-based inhibitory motif</topic><topic>KIR killer cell-inhibitory receptors</topic><topic>MHC rodent</topic><topic>MLR mixed lymphocyte reaction</topic><topic>PE phycoerythrin</topic><topic>SAvRED streptavidin–Red670</topic><topic>TGF transforming growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lohwasser, Stefan</creatorcontrib><creatorcontrib>Kubota, Akira</creatorcontrib><creatorcontrib>Salcedo, Margarita</creatorcontrib><creatorcontrib>Lian, Rebecca H.</creatorcontrib><creatorcontrib>Takei1, Fumio</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lohwasser, Stefan</au><au>Kubota, Akira</au><au>Salcedo, Margarita</au><au>Lian, Rebecca H.</au><au>Takei1, Fumio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The non-classical MHC class I molecule Qa-1b inhibits classical MHC class I-restricted cytotoxicity of cytotoxic T lymphocytes</atitle><jtitle>International immunology</jtitle><addtitle>Int. Immunol</addtitle><date>2001-03</date><risdate>2001</risdate><volume>13</volume><issue>3</issue><spage>321</spage><epage>327</epage><pages>321-327</pages><issn>0953-8178</issn><eissn>1460-2377</eissn><abstract>The CD94/NKG2A heterodimer is an inhibitory receptor expressed on a subset of mouse NK cells. CD94/NKG2A recognizes the non-classical MHC class I (class Ib) molecule Qa-1b and inhibits NK cytotoxicity. Qa-1b presents a peptide derived from the leader sequence of classical MHC class I molecules. Here, we examined the role of CD94/NKG2A in T cell-mediated cytotoxicity. Soluble tetrameric Qa-1b bound to almost all CD8+, but not CD4+, T cells. This binding seems to be mediated by CD8, because COS cells transfected with CD8 also bound Qa-1b tetramer. Therefore, the expression of CD94/NKG2 in T cells was further examined by single-cell RT-PCR. Most murine CD8+ T cells constitutively expressed CD94 and NKG2A transcripts, whereas they were not detected in CD4+ T cells. Co-expression of Qa-1b and Dk on target cells significantly inhibited cytotoxicity of Dk-specific cytotoxic T lymphocytes generated by mixed lymphocyte reaction, indicating that Qa-1b on antigen-presenting cells interacts with CD94/NKG2A on CD8 T cells and regulates classical MHC class I-restricted cytotoxic T cells. These results suggest a significant role of CD94/NKG2A as an inhibitory receptor on CD8+ T cells.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><doi>10.1093/intimm/13.3.321</doi><tpages>7</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | APC allophycocyanin cell-cell interactions CTL cytotoxic T lymphocyte cytotoxic T lymphocyte GFP green fluorescence protein ITIM immunoreceptor tyrosine-based inhibitory motif KIR killer cell-inhibitory receptors MHC rodent MLR mixed lymphocyte reaction PE phycoerythrin SAvRED streptavidin–Red670 TGF transforming growth factor |
| title | The non-classical MHC class I molecule Qa-1b inhibits classical MHC class I-restricted cytotoxicity of cytotoxic T lymphocytes |
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