An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection
An aptamer-based colorimetric biosensor was developed, which could be used to detect residual strepto- mycin from food quickly and cost-effectively. The ssDNA aptamer target for streptomycin was obtained using sys- temic evolution of ligands by exponential enrichment through affinity chromatography....
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| Veröffentlicht in: | Chemical research in Chinese universities 2017-10, Vol.33 (5), p.714-720 |
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| creator | Liu, Zhongcheng Zhang, Yanfen Xie, Yao Sun, Ying Bi, Kewei Cui, Zhe Zhao, Lijian Fan, Wufang |
| description | An aptamer-based colorimetric biosensor was developed, which could be used to detect residual strepto- mycin from food quickly and cost-effectively. The ssDNA aptamer target for streptomycin was obtained using sys- temic evolution of ligands by exponential enrichment through affinity chromatography. A total of 19 candidates were obtained after 10 rounds of selection and were grouped into 3 families according to their similarity of sequence and structure. Among them, aptamer A15 showed the highest affinity for streptomycin tested by fluorescence intensity analysis with a dissociation constant of 6.07 nmol/L. Aptamer A15 also exhibited a higher streptomycin specificity with the lowest detectable limit of 25 nmol/L according to the value of A620/A520 ratio with AuNP-based colorimetric assay. The method was specific and sensitive for the detection of streptomycin from simply-treated milk and honey at 100 and 125 nmol/L, respectively, and is a promising approach to monitor antibiotics or other small molecules ana- logues in food inspection. |
| doi_str_mv | 10.1007/s40242-017-7029-6 |
| format | Article |
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The ssDNA aptamer target for streptomycin was obtained using sys- temic evolution of ligands by exponential enrichment through affinity chromatography. A total of 19 candidates were obtained after 10 rounds of selection and were grouped into 3 families according to their similarity of sequence and structure. Among them, aptamer A15 showed the highest affinity for streptomycin tested by fluorescence intensity analysis with a dissociation constant of 6.07 nmol/L. Aptamer A15 also exhibited a higher streptomycin specificity with the lowest detectable limit of 25 nmol/L according to the value of A620/A520 ratio with AuNP-based colorimetric assay. The method was specific and sensitive for the detection of streptomycin from simply-treated milk and honey at 100 and 125 nmol/L, respectively, and is a promising approach to monitor antibiotics or other small molecules ana- logues in food inspection.</description><identifier>ISSN: 1005-9040</identifier><identifier>EISSN: 2210-3171</identifier><identifier>DOI: 10.1007/s40242-017-7029-6</identifier><language>eng</language><publisher>Changchun: Jilin University and The Editorial Department of Chemical Research in Chinese Universities</publisher><subject>Affinity ; Analytical Chemistry ; Antibiotics ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Colorimetry ; Fluorescence ; Inorganic Chemistry ; Inspection ; Milk ; Organic Chemistry ; Physical Chemistry ; Streptomycin ; 亲和层析 ; 使用系统 ; 单链DNA ; 应用 ; 色传感器 ; 适体 ; 链霉素 ; 食品检测</subject><ispartof>Chemical research in Chinese universities, 2017-10, Vol.33 (5), p.714-720</ispartof><rights>Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH Germany 2017</rights><rights>Copyright Springer Science & Business Media 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-490db2e176ec29cdd5ca6ac0899079dba784956202919423e6ed7de3603b551a3</citedby><cites>FETCH-LOGICAL-c343t-490db2e176ec29cdd5ca6ac0899079dba784956202919423e6ed7de3603b551a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86071X/86071X.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40242-017-7029-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40242-017-7029-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Liu, Zhongcheng</creatorcontrib><creatorcontrib>Zhang, Yanfen</creatorcontrib><creatorcontrib>Xie, Yao</creatorcontrib><creatorcontrib>Sun, Ying</creatorcontrib><creatorcontrib>Bi, Kewei</creatorcontrib><creatorcontrib>Cui, Zhe</creatorcontrib><creatorcontrib>Zhao, Lijian</creatorcontrib><creatorcontrib>Fan, Wufang</creatorcontrib><title>An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection</title><title>Chemical research in Chinese universities</title><addtitle>Chem. Res. Chin. Univ</addtitle><addtitle>Chemical Research in Chinese University</addtitle><description>An aptamer-based colorimetric biosensor was developed, which could be used to detect residual strepto- mycin from food quickly and cost-effectively. The ssDNA aptamer target for streptomycin was obtained using sys- temic evolution of ligands by exponential enrichment through affinity chromatography. A total of 19 candidates were obtained after 10 rounds of selection and were grouped into 3 families according to their similarity of sequence and structure. Among them, aptamer A15 showed the highest affinity for streptomycin tested by fluorescence intensity analysis with a dissociation constant of 6.07 nmol/L. Aptamer A15 also exhibited a higher streptomycin specificity with the lowest detectable limit of 25 nmol/L according to the value of A620/A520 ratio with AuNP-based colorimetric assay. The method was specific and sensitive for the detection of streptomycin from simply-treated milk and honey at 100 and 125 nmol/L, respectively, and is a promising approach to monitor antibiotics or other small molecules ana- logues in food inspection.</description><subject>Affinity</subject><subject>Analytical Chemistry</subject><subject>Antibiotics</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Colorimetry</subject><subject>Fluorescence</subject><subject>Inorganic Chemistry</subject><subject>Inspection</subject><subject>Milk</subject><subject>Organic Chemistry</subject><subject>Physical Chemistry</subject><subject>Streptomycin</subject><subject>亲和层析</subject><subject>使用系统</subject><subject>单链DNA</subject><subject>应用</subject><subject>色传感器</subject><subject>适体</subject><subject>链霉素</subject><subject>食品检测</subject><issn>1005-9040</issn><issn>2210-3171</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEYhIMoWKs_wNui5-ibj02aYylWCwUP1ZsQ0mxat7TJNkkP_fembBFPHkJgmOcdZhC6J_BEAORz4kA5xUAklkAVFhdoQCkBzIgkl2hQTDVWwOEa3aS0AWBKCD5AX2Nfjbtsdi7ipUmuqSZhG2K7czm2tlo4n0KsVuUtcnRdDrujbX1lfFPNcipot22tyW3wVZGnIRTdp87Zk3SLrlZmm9zd-R-iz-nLx-QNz99fZ5PxHFvGWcZcQbOkjkjhLFW2aWprhLEwUgqkapZGjriqBS29iOKUOeEa2TgmgC3rmhg2RI_93S6G_cGlrDfhEH2J1AWQpTvlpLhI77IxpBTdSnelp4lHTUCfRtT9iLqMqE8jalEY2jOpeP3axT-X_4EezkHfwa_3hftNEpIxKZlU7Acsv39d</recordid><startdate>20171001</startdate><enddate>20171001</enddate><creator>Liu, Zhongcheng</creator><creator>Zhang, Yanfen</creator><creator>Xie, Yao</creator><creator>Sun, Ying</creator><creator>Bi, Kewei</creator><creator>Cui, Zhe</creator><creator>Zhao, Lijian</creator><creator>Fan, Wufang</creator><general>Jilin University and The Editorial Department of Chemical Research in Chinese Universities</general><general>Springer Nature B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20171001</creationdate><title>An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection</title><author>Liu, Zhongcheng ; Zhang, Yanfen ; Xie, Yao ; Sun, Ying ; Bi, Kewei ; Cui, Zhe ; Zhao, Lijian ; Fan, Wufang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-490db2e176ec29cdd5ca6ac0899079dba784956202919423e6ed7de3603b551a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Affinity</topic><topic>Analytical Chemistry</topic><topic>Antibiotics</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Colorimetry</topic><topic>Fluorescence</topic><topic>Inorganic Chemistry</topic><topic>Inspection</topic><topic>Milk</topic><topic>Organic Chemistry</topic><topic>Physical Chemistry</topic><topic>Streptomycin</topic><topic>亲和层析</topic><topic>使用系统</topic><topic>单链DNA</topic><topic>应用</topic><topic>色传感器</topic><topic>适体</topic><topic>链霉素</topic><topic>食品检测</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Zhongcheng</creatorcontrib><creatorcontrib>Zhang, Yanfen</creatorcontrib><creatorcontrib>Xie, Yao</creatorcontrib><creatorcontrib>Sun, Ying</creatorcontrib><creatorcontrib>Bi, Kewei</creatorcontrib><creatorcontrib>Cui, Zhe</creatorcontrib><creatorcontrib>Zhao, Lijian</creatorcontrib><creatorcontrib>Fan, Wufang</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><jtitle>Chemical research in Chinese universities</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Zhongcheng</au><au>Zhang, Yanfen</au><au>Xie, Yao</au><au>Sun, Ying</au><au>Bi, Kewei</au><au>Cui, Zhe</au><au>Zhao, Lijian</au><au>Fan, Wufang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection</atitle><jtitle>Chemical research in Chinese universities</jtitle><stitle>Chem. 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| subjects | Affinity Analytical Chemistry Antibiotics Chemistry Chemistry and Materials Science Chemistry/Food Science Colorimetry Fluorescence Inorganic Chemistry Inspection Milk Organic Chemistry Physical Chemistry Streptomycin 亲和层析 使用系统 单链DNA 应用 色传感器 适体 链霉素 食品检测 |
| title | An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection |
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