Alterations in the proteome of wheat primary roots after wortmannin application during seed germination
Wortmannin is an efficient inhibitor of phosphatidylinositol 3-kinases that is involved in normal plant growth. In this study, we performed the global proteomic analysis of the effects of wortmannin on wheat primary roots during seed germination. Wortmannin significantly inhibited the growth of whea...
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| Veröffentlicht in: | Acta physiologiae plantarum 2017-10, Vol.39 (10), p.1-21, Article 223 |
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| creator | Cao, Hui Ni, Xiaolin Zhang, Caiyun Shi, Wenshuo Xu, Yuxing Yan, Yueming Zhang, Feixiong |
| description | Wortmannin is an efficient inhibitor of phosphatidylinositol 3-kinases that is involved in normal plant growth. In this study, we performed the global proteomic analysis of the effects of wortmannin on wheat primary roots during seed germination. Wortmannin significantly inhibited the growth of wheat primary roots in a macroscopic level, and damaged the fine structure of nucleoli and mitochondria in a microscopic level. In total, 75 differentially accumulated protein (DAP) spots representing 66 unique DAPs were identified by linear two-dimensional electrophoresis and tandem mass spectrometry. These DAPs were mainly related to detoxification and defense response, carbon metabolism, and amino acid metabolism. Principal component analysis indicated that wortmannin significantly induced the proteomic changes between the treatment and control groups. Protein–protein interaction analysis revealed a complex network centralized by the 14-3-3 protein which was significantly upregulated in both transcriptional and translational levels. Furthermore, some key DAPs, such as heat shock-related proteins, glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase, 5-methyltetrahydropteroyltriglutamate–homocysteine methyltransferase, and elongation factor, also showed significantly upregulated accumulation, indicating their key roles in cell homeostasis maintenance, energy supply, and protein biosynthesis. Results obtained from this study provided a new insight into the molecular mechanism of wheat primary root in response to wortmannin during seed germination. |
| doi_str_mv | 10.1007/s11738-017-2511-9 |
| format | Article |
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In this study, we performed the global proteomic analysis of the effects of wortmannin on wheat primary roots during seed germination. Wortmannin significantly inhibited the growth of wheat primary roots in a macroscopic level, and damaged the fine structure of nucleoli and mitochondria in a microscopic level. In total, 75 differentially accumulated protein (DAP) spots representing 66 unique DAPs were identified by linear two-dimensional electrophoresis and tandem mass spectrometry. These DAPs were mainly related to detoxification and defense response, carbon metabolism, and amino acid metabolism. Principal component analysis indicated that wortmannin significantly induced the proteomic changes between the treatment and control groups. Protein–protein interaction analysis revealed a complex network centralized by the 14-3-3 protein which was significantly upregulated in both transcriptional and translational levels. Furthermore, some key DAPs, such as heat shock-related proteins, glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase, 5-methyltetrahydropteroyltriglutamate–homocysteine methyltransferase, and elongation factor, also showed significantly upregulated accumulation, indicating their key roles in cell homeostasis maintenance, energy supply, and protein biosynthesis. Results obtained from this study provided a new insight into the molecular mechanism of wheat primary root in response to wortmannin during seed germination.</description><identifier>ISSN: 0137-5881</identifier><identifier>EISSN: 1861-1664</identifier><identifier>DOI: 10.1007/s11738-017-2511-9</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>14-3-3 protein ; Agriculture ; Amino acids ; Biomedical and Life Sciences ; Biosynthesis ; Dehydrogenase ; Dehydrogenases ; Detoxification ; Elongation ; Fine structure ; Germination ; Glyceraldehyde ; Glyceraldehyde-3-phosphate dehydrogenase ; Heat shock ; Homeostasis ; Homocysteine ; Isocitrate dehydrogenase ; Kinases ; Life Sciences ; Mass spectrometry ; Mass spectroscopy ; Metabolism ; Methyltransferase ; Mitochondria ; Nucleoli ; Original Article ; Phosphates ; Phosphatidylinositol ; Plant Anatomy/Development ; Plant Biochemistry ; Plant Genetics and Genomics ; Plant growth ; Plant Pathology ; Plant Physiology ; Principal components analysis ; Protein biosynthesis ; Proteins ; Proteomics ; Roots ; Seed germination ; Spots ; Structural damage ; Transcription ; Ultrastructure ; Wheat ; Wortmannin</subject><ispartof>Acta physiologiae plantarum, 2017-10, Vol.39 (10), p.1-21, Article 223</ispartof><rights>Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2017</rights><rights>Copyright Springer Science & Business Media 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-c72c3501f344e6ab0057ed39cc1cf088efafe3d321a0ca08167ab7e7c443785c3</citedby><cites>FETCH-LOGICAL-c316t-c72c3501f344e6ab0057ed39cc1cf088efafe3d321a0ca08167ab7e7c443785c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11738-017-2511-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11738-017-2511-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Cao, Hui</creatorcontrib><creatorcontrib>Ni, Xiaolin</creatorcontrib><creatorcontrib>Zhang, Caiyun</creatorcontrib><creatorcontrib>Shi, Wenshuo</creatorcontrib><creatorcontrib>Xu, Yuxing</creatorcontrib><creatorcontrib>Yan, Yueming</creatorcontrib><creatorcontrib>Zhang, Feixiong</creatorcontrib><title>Alterations in the proteome of wheat primary roots after wortmannin application during seed germination</title><title>Acta physiologiae plantarum</title><addtitle>Acta Physiol Plant</addtitle><description>Wortmannin is an efficient inhibitor of phosphatidylinositol 3-kinases that is involved in normal plant growth. In this study, we performed the global proteomic analysis of the effects of wortmannin on wheat primary roots during seed germination. Wortmannin significantly inhibited the growth of wheat primary roots in a macroscopic level, and damaged the fine structure of nucleoli and mitochondria in a microscopic level. In total, 75 differentially accumulated protein (DAP) spots representing 66 unique DAPs were identified by linear two-dimensional electrophoresis and tandem mass spectrometry. These DAPs were mainly related to detoxification and defense response, carbon metabolism, and amino acid metabolism. Principal component analysis indicated that wortmannin significantly induced the proteomic changes between the treatment and control groups. Protein–protein interaction analysis revealed a complex network centralized by the 14-3-3 protein which was significantly upregulated in both transcriptional and translational levels. Furthermore, some key DAPs, such as heat shock-related proteins, glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase, 5-methyltetrahydropteroyltriglutamate–homocysteine methyltransferase, and elongation factor, also showed significantly upregulated accumulation, indicating their key roles in cell homeostasis maintenance, energy supply, and protein biosynthesis. Results obtained from this study provided a new insight into the molecular mechanism of wheat primary root in response to wortmannin during seed germination.</description><subject>14-3-3 protein</subject><subject>Agriculture</subject><subject>Amino acids</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Dehydrogenase</subject><subject>Dehydrogenases</subject><subject>Detoxification</subject><subject>Elongation</subject><subject>Fine structure</subject><subject>Germination</subject><subject>Glyceraldehyde</subject><subject>Glyceraldehyde-3-phosphate dehydrogenase</subject><subject>Heat shock</subject><subject>Homeostasis</subject><subject>Homocysteine</subject><subject>Isocitrate dehydrogenase</subject><subject>Kinases</subject><subject>Life Sciences</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolism</subject><subject>Methyltransferase</subject><subject>Mitochondria</subject><subject>Nucleoli</subject><subject>Original Article</subject><subject>Phosphates</subject><subject>Phosphatidylinositol</subject><subject>Plant Anatomy/Development</subject><subject>Plant Biochemistry</subject><subject>Plant Genetics and Genomics</subject><subject>Plant growth</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Principal components analysis</subject><subject>Protein biosynthesis</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Roots</subject><subject>Seed germination</subject><subject>Spots</subject><subject>Structural damage</subject><subject>Transcription</subject><subject>Ultrastructure</subject><subject>Wheat</subject><subject>Wortmannin</subject><issn>0137-5881</issn><issn>1861-1664</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp1kE9LAzEQxYMoWKsfwFvAczSz2U2yx1L8BwUveg5pdrbd0t3UJKX47U27PXjxNPCY35t5j5B74I_AuXqKAEpoxkGxogJg9QWZgJbAQMrykkw4CMUqreGa3MS44bwSlZQTspptEwabOj9E2g00rZHugk_oe6S-pYc12pSVrrfhhwbvU6S2zQg9-JB6OwwZsrvdtnMnE9rsQzesaERs6ApD3w0n_ZZctXYb8e48p-Tr5flz_sYWH6_v89mCOQEyMacKJyoOrShLlHaZ_1TYiNo5cC3XGlvbomhEAZY7yzVIZZcKlStLoXTlxJQ8jL45xPceYzIbvw9DPmmgFrLSpahV3oJxywUfY8DWnBMa4ObYpxn7NLlPc-zT1JkpRibujgkx_HH-F_oF19p6Yg</recordid><startdate>20171001</startdate><enddate>20171001</enddate><creator>Cao, Hui</creator><creator>Ni, Xiaolin</creator><creator>Zhang, Caiyun</creator><creator>Shi, Wenshuo</creator><creator>Xu, Yuxing</creator><creator>Yan, Yueming</creator><creator>Zhang, Feixiong</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20171001</creationdate><title>Alterations in the proteome of wheat primary roots after wortmannin application during seed germination</title><author>Cao, Hui ; Ni, Xiaolin ; Zhang, Caiyun ; Shi, Wenshuo ; Xu, Yuxing ; Yan, Yueming ; Zhang, Feixiong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-c72c3501f344e6ab0057ed39cc1cf088efafe3d321a0ca08167ab7e7c443785c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>14-3-3 protein</topic><topic>Agriculture</topic><topic>Amino acids</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Dehydrogenase</topic><topic>Dehydrogenases</topic><topic>Detoxification</topic><topic>Elongation</topic><topic>Fine structure</topic><topic>Germination</topic><topic>Glyceraldehyde</topic><topic>Glyceraldehyde-3-phosphate dehydrogenase</topic><topic>Heat shock</topic><topic>Homeostasis</topic><topic>Homocysteine</topic><topic>Isocitrate dehydrogenase</topic><topic>Kinases</topic><topic>Life Sciences</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolism</topic><topic>Methyltransferase</topic><topic>Mitochondria</topic><topic>Nucleoli</topic><topic>Original Article</topic><topic>Phosphates</topic><topic>Phosphatidylinositol</topic><topic>Plant Anatomy/Development</topic><topic>Plant Biochemistry</topic><topic>Plant Genetics and Genomics</topic><topic>Plant growth</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Principal components analysis</topic><topic>Protein biosynthesis</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Roots</topic><topic>Seed germination</topic><topic>Spots</topic><topic>Structural damage</topic><topic>Transcription</topic><topic>Ultrastructure</topic><topic>Wheat</topic><topic>Wortmannin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cao, Hui</creatorcontrib><creatorcontrib>Ni, Xiaolin</creatorcontrib><creatorcontrib>Zhang, Caiyun</creatorcontrib><creatorcontrib>Shi, Wenshuo</creatorcontrib><creatorcontrib>Xu, Yuxing</creatorcontrib><creatorcontrib>Yan, Yueming</creatorcontrib><creatorcontrib>Zhang, Feixiong</creatorcontrib><collection>CrossRef</collection><jtitle>Acta physiologiae plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cao, Hui</au><au>Ni, Xiaolin</au><au>Zhang, Caiyun</au><au>Shi, Wenshuo</au><au>Xu, Yuxing</au><au>Yan, Yueming</au><au>Zhang, Feixiong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alterations in the proteome of wheat primary roots after wortmannin application during seed germination</atitle><jtitle>Acta physiologiae plantarum</jtitle><stitle>Acta Physiol Plant</stitle><date>2017-10-01</date><risdate>2017</risdate><volume>39</volume><issue>10</issue><spage>1</spage><epage>21</epage><pages>1-21</pages><artnum>223</artnum><issn>0137-5881</issn><eissn>1861-1664</eissn><abstract>Wortmannin is an efficient inhibitor of phosphatidylinositol 3-kinases that is involved in normal plant growth. In this study, we performed the global proteomic analysis of the effects of wortmannin on wheat primary roots during seed germination. Wortmannin significantly inhibited the growth of wheat primary roots in a macroscopic level, and damaged the fine structure of nucleoli and mitochondria in a microscopic level. In total, 75 differentially accumulated protein (DAP) spots representing 66 unique DAPs were identified by linear two-dimensional electrophoresis and tandem mass spectrometry. These DAPs were mainly related to detoxification and defense response, carbon metabolism, and amino acid metabolism. Principal component analysis indicated that wortmannin significantly induced the proteomic changes between the treatment and control groups. Protein–protein interaction analysis revealed a complex network centralized by the 14-3-3 protein which was significantly upregulated in both transcriptional and translational levels. Furthermore, some key DAPs, such as heat shock-related proteins, glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase, 5-methyltetrahydropteroyltriglutamate–homocysteine methyltransferase, and elongation factor, also showed significantly upregulated accumulation, indicating their key roles in cell homeostasis maintenance, energy supply, and protein biosynthesis. Results obtained from this study provided a new insight into the molecular mechanism of wheat primary root in response to wortmannin during seed germination.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s11738-017-2511-9</doi><tpages>21</tpages></addata></record> |
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| subjects | 14-3-3 protein Agriculture Amino acids Biomedical and Life Sciences Biosynthesis Dehydrogenase Dehydrogenases Detoxification Elongation Fine structure Germination Glyceraldehyde Glyceraldehyde-3-phosphate dehydrogenase Heat shock Homeostasis Homocysteine Isocitrate dehydrogenase Kinases Life Sciences Mass spectrometry Mass spectroscopy Metabolism Methyltransferase Mitochondria Nucleoli Original Article Phosphates Phosphatidylinositol Plant Anatomy/Development Plant Biochemistry Plant Genetics and Genomics Plant growth Plant Pathology Plant Physiology Principal components analysis Protein biosynthesis Proteins Proteomics Roots Seed germination Spots Structural damage Transcription Ultrastructure Wheat Wortmannin |
| title | Alterations in the proteome of wheat primary roots after wortmannin application during seed germination |
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