Genetic diversity of common bean genotypes of Carioca commercial group using RAPD markers
The objective of this research was to characterize the genetic diversity of bean genotypes (Phaseolus vulgaris L.), from Carioca commercial group, utilizing RAPD markers to discriminate and to support breeders to choose genetic material and to develop breeding methods to use for obtaining new cultiv...
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Veröffentlicht in: | Crop breeding and applied biotechnology 2005-03, Vol.5 (1), p.80-85 |
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creator | Palomino, E.C. Mori, E.S. Zimback, L. Tambarussi, E.V. Moraes, C.B. |
description | The objective of this research was to characterize the genetic diversity of bean genotypes (Phaseolus vulgaris L.), from Carioca commercial group, utilizing RAPD markers to discriminate and to support breeders to choose genetic material and to develop breeding methods to use for obtaining new cultivars with Carioca type seeds. Twenty-eight genotypes, fifteen cultivars, and thirteen lines were evaluated. The genetic distances were achieved utilizing the Euclidian’s methodmodified by Roger’s (1972) and the grouping analysis were by UPGMA method, showed by a dendogram. Through 89 RAPD loci, obtained from 12 primers, 65.17% were polymorphics. We have verified that the RAPD markers were efficient in discrimination and grouping of genotypes, showing to have large genetic variability. Although Carioca cultivar has a common ancestor from all studied material, it has shown low similarity with genotypes derived from it, corroborating to exist sufficient genetic variability for production of new cultivars, maintaining the original coloration of seeds |
doi_str_mv | 10.12702/1984-7033.v05n01a10 |
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Twenty-eight genotypes, fifteen cultivars, and thirteen lines were evaluated. The genetic distances were achieved utilizing the Euclidian’s methodmodified by Roger’s (1972) and the grouping analysis were by UPGMA method, showed by a dendogram. Through 89 RAPD loci, obtained from 12 primers, 65.17% were polymorphics. We have verified that the RAPD markers were efficient in discrimination and grouping of genotypes, showing to have large genetic variability. 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Twenty-eight genotypes, fifteen cultivars, and thirteen lines were evaluated. The genetic distances were achieved utilizing the Euclidian’s methodmodified by Roger’s (1972) and the grouping analysis were by UPGMA method, showed by a dendogram. Through 89 RAPD loci, obtained from 12 primers, 65.17% were polymorphics. We have verified that the RAPD markers were efficient in discrimination and grouping of genotypes, showing to have large genetic variability. Although Carioca cultivar has a common ancestor from all studied material, it has shown low similarity with genotypes derived from it, corroborating to exist sufficient genetic variability for production of new cultivars, maintaining the original coloration of seeds</description><subject>Beans</subject><subject>Breeding methods</subject><subject>Coloration</subject><subject>Cultivars</subject><subject>Genetic distance</subject><subject>Genetic diversity</subject><subject>Genetic variability</subject><subject>Genotypes</subject><subject>Markers</subject><subject>Phaseolus vulgaris</subject><subject>Plant breeding</subject><subject>Random amplified polymorphic DNA</subject><subject>Seeds</subject><issn>1984-7033</issn><issn>1518-7853</issn><issn>1984-7033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpNkE1LAzEQhoMoWKv_wEPA89ZMvnb3WKpWoaCIHjyFbJqUrW2yJruF_fduWymeZph5eGd4ELoFMgGaE3oPZcGznDA22RHhCWggZ2h0mp7_6y_RVUprQriQZTlCX3PrbVsbvKx3Nqa67XFw2ITtNnhcWe3xyvrQ9o1N-8VMxzoYfQBsNLXe4FUMXYO7VPsVfp--PeCtjt9D1DW6cHqT7M1fHaPPp8eP2XO2eJ2_zKaLzADlMqMlk9JWhRMu59wtqXRa5IUhYAtWicppRoBWZmlEYbWshKYUBOfalBK4BDZGd8fcJoafzqZWrUMX_XBSwZDNSsiFHCh-pEwMKUXrVBPr4dNeAVEHiWqvSO0VqZNE9guPn2Vt</recordid><startdate>20050330</startdate><enddate>20050330</enddate><creator>Palomino, E.C.</creator><creator>Mori, E.S.</creator><creator>Zimback, L.</creator><creator>Tambarussi, E.V.</creator><creator>Moraes, C.B.</creator><general>Crop Breeding and Applied Biotechnology</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>M7S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope></search><sort><creationdate>20050330</creationdate><title>Genetic diversity of common bean genotypes of Carioca commercial group using RAPD markers</title><author>Palomino, E.C. ; 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Twenty-eight genotypes, fifteen cultivars, and thirteen lines were evaluated. The genetic distances were achieved utilizing the Euclidian’s methodmodified by Roger’s (1972) and the grouping analysis were by UPGMA method, showed by a dendogram. Through 89 RAPD loci, obtained from 12 primers, 65.17% were polymorphics. We have verified that the RAPD markers were efficient in discrimination and grouping of genotypes, showing to have large genetic variability. Although Carioca cultivar has a common ancestor from all studied material, it has shown low similarity with genotypes derived from it, corroborating to exist sufficient genetic variability for production of new cultivars, maintaining the original coloration of seeds</abstract><cop>Vicosa</cop><pub>Crop Breeding and Applied Biotechnology</pub><doi>10.12702/1984-7033.v05n01a10</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
subjects | Beans Breeding methods Coloration Cultivars Genetic distance Genetic diversity Genetic variability Genotypes Markers Phaseolus vulgaris Plant breeding Random amplified polymorphic DNA Seeds |
title | Genetic diversity of common bean genotypes of Carioca commercial group using RAPD markers |
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