In vitro and in vivo anti-Vibrio vulnificus activity of psammaplin A, a natural marine compound
Vibrio vulnificus is known to induce severely fulminant and fatal septicemia in susceptible hosts. In the present study, the antimicrobial activity of natural marine product-derived compounds against V. vulnificus, were investigated in vitro and in vivo. Twelve pure compounds were isolated from natu...
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| Veröffentlicht in: | Molecular medicine reports 2016-09, Vol.14 (3), p.2691-2696 |
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| creator | Lee, Byung Cheol Lee, Arim Jung, Jee Hyung Choi, Sang Ho Kim, Tae Sung |
| description | Vibrio vulnificus is known to induce severely fulminant and fatal septicemia in susceptible hosts. In the present study, the antimicrobial activity of natural marine product-derived compounds against V. vulnificus, were investigated in vitro and in vivo. Twelve pure compounds were isolated from natural marine products and their inhibitory effects on V. vulnificus-induced cytotoxicity were determined in INT-407 cells. Among the 12 pure compounds tested, treatment with psammaplin A significantly suppressed V. vulnificus-induced cytotoxicity in INT-407 cells. Notably, treatment with psammaplin A (5-50 μg) had improved survival rates compared with that in the untreated mice, when the mice were infected with V. vulnificus intraperitoneally. In addition, the bacterial load of V. vulnificus in several tissues (spleen, liver and small intestine) was significantly lower in psammaplin A-treated mice than in untreated mice. Furthermore, psammaplin A treatment significantly suppressed the growth of V. vulnificus. Taken together, these results indicate that psammaplin A may be a potential agent for the prevention and treatment of V. vulnificus infections. |
| doi_str_mv | 10.3892/mmr.2016.5522 |
| format | Article |
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In the present study, the antimicrobial activity of natural marine product-derived compounds against V. vulnificus, were investigated in vitro and in vivo. Twelve pure compounds were isolated from natural marine products and their inhibitory effects on V. vulnificus-induced cytotoxicity were determined in INT-407 cells. Among the 12 pure compounds tested, treatment with psammaplin A significantly suppressed V. vulnificus-induced cytotoxicity in INT-407 cells. Notably, treatment with psammaplin A (5-50 μg) had improved survival rates compared with that in the untreated mice, when the mice were infected with V. vulnificus intraperitoneally. In addition, the bacterial load of V. vulnificus in several tissues (spleen, liver and small intestine) was significantly lower in psammaplin A-treated mice than in untreated mice. Furthermore, psammaplin A treatment significantly suppressed the growth of V. vulnificus. Taken together, these results indicate that psammaplin A may be a potential agent for the prevention and treatment of V. vulnificus infections.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2016.5522</identifier><identifier>PMID: 27431807</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antimicrobial activity ; Antimicrobial agents ; Apoptosis ; Aquatic Organisms - chemistry ; Bacteria ; Biological Products - pharmacology ; Care and treatment ; Cell Line ; Cytotoxicity ; Deoxyribonucleic acid ; Disease Models, Animal ; Disulfides - pharmacology ; DNA ; Epithelial Cells ; Female ; Fish ; Gene expression ; Gram-positive bacteria ; Health aspects ; Humans ; Infections ; Liver ; Mice ; Microbial Sensitivity Tests ; Molecular Structure ; Natural products ; psammaplin A ; Sepsis ; Septicemia ; Small intestine ; Spleen ; Studies ; Tumor necrosis factor-TNF ; Tyrosine - analogs & derivatives ; Tyrosine - pharmacology ; Vibrio infections ; Vibrio Infections - drug therapy ; Vibrio Infections - microbiology ; Vibrio Infections - mortality ; Vibrio Infections - pathology ; Vibrio vulnificus ; Vibrio vulnificus - drug effects</subject><ispartof>Molecular medicine reports, 2016-09, Vol.14 (3), p.2691-2696</ispartof><rights>Copyright © 2016, Spandidos Publications</rights><rights>COPYRIGHT 2016 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-b23590c27013da92e333460740b5af59065c7f7dd0b94acacc662f7d2c3dbde53</citedby><cites>FETCH-LOGICAL-c459t-b23590c27013da92e333460740b5af59065c7f7dd0b94acacc662f7d2c3dbde53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,5573,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27431807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Byung Cheol</creatorcontrib><creatorcontrib>Lee, Arim</creatorcontrib><creatorcontrib>Jung, Jee Hyung</creatorcontrib><creatorcontrib>Choi, Sang Ho</creatorcontrib><creatorcontrib>Kim, Tae Sung</creatorcontrib><title>In vitro and in vivo anti-Vibrio vulnificus activity of psammaplin A, a natural marine compound</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Vibrio vulnificus is known to induce severely fulminant and fatal septicemia in susceptible hosts. In the present study, the antimicrobial activity of natural marine product-derived compounds against V. vulnificus, were investigated in vitro and in vivo. Twelve pure compounds were isolated from natural marine products and their inhibitory effects on V. vulnificus-induced cytotoxicity were determined in INT-407 cells. Among the 12 pure compounds tested, treatment with psammaplin A significantly suppressed V. vulnificus-induced cytotoxicity in INT-407 cells. Notably, treatment with psammaplin A (5-50 μg) had improved survival rates compared with that in the untreated mice, when the mice were infected with V. vulnificus intraperitoneally. In addition, the bacterial load of V. vulnificus in several tissues (spleen, liver and small intestine) was significantly lower in psammaplin A-treated mice than in untreated mice. Furthermore, psammaplin A treatment significantly suppressed the growth of V. vulnificus. Taken together, these results indicate that psammaplin A may be a potential agent for the prevention and treatment of V. vulnificus infections.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Apoptosis</subject><subject>Aquatic Organisms - chemistry</subject><subject>Bacteria</subject><subject>Biological Products - pharmacology</subject><subject>Care and treatment</subject><subject>Cell Line</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>Disease Models, Animal</subject><subject>Disulfides - pharmacology</subject><subject>DNA</subject><subject>Epithelial Cells</subject><subject>Female</subject><subject>Fish</subject><subject>Gene expression</subject><subject>Gram-positive bacteria</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Infections</subject><subject>Liver</subject><subject>Mice</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Structure</subject><subject>Natural products</subject><subject>psammaplin A</subject><subject>Sepsis</subject><subject>Septicemia</subject><subject>Small intestine</subject><subject>Spleen</subject><subject>Studies</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tyrosine - analogs & derivatives</subject><subject>Tyrosine - pharmacology</subject><subject>Vibrio infections</subject><subject>Vibrio Infections - drug therapy</subject><subject>Vibrio Infections - microbiology</subject><subject>Vibrio Infections - mortality</subject><subject>Vibrio Infections - pathology</subject><subject>Vibrio vulnificus</subject><subject>Vibrio vulnificus - drug effects</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkU2PFCEQQDtG437o0ash8eBFRiigGY6TjbqbbOJFvRIaaMOmgRa6J9l_L50Z15gYDlTBqyrC67o3lOzYXsHHGMsOCO13QgA86y6pVBQzQvjzcwxKyYvuqtYHQnoBQr3sLkByRvdEXnb6LqFjWEpGJjkUtuS4xUvAP8JQQkbHdUphDHatyNglNPgR5RHN1cRo5qmVHD4gg5JZ1mImFE0JySOb45zX5F51L0YzVf_6vF933z9_-nZzi--_frm7Odxjy4Va8ABMKGJBEsqcUeAZY7wnkpNBmLFd9cLKUTpHBsWNNdb2PbQcLHOD84Jdd-9OfeeSf62-LvohryW1kZoqBlxKBfQv9dNMXoc05qUYG0O1-sB7UHsQQBq1-w_VlvMx2Jz8GNr5PwX4VGBLrrX4Uc8ltI941JTozZJulvRmSW-WGv_2_Nh1iN490X-0NOD9Cahz0xJcrk9M64Qpx4Rh6BVlvwE40pjn</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Lee, Byung Cheol</creator><creator>Lee, Arim</creator><creator>Jung, Jee Hyung</creator><creator>Choi, Sang Ho</creator><creator>Kim, Tae Sung</creator><general>D.A. 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Lee, Arim ; Jung, Jee Hyung ; Choi, Sang Ho ; Kim, Tae Sung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-b23590c27013da92e333460740b5af59065c7f7dd0b94acacc662f7d2c3dbde53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>Apoptosis</topic><topic>Aquatic Organisms - chemistry</topic><topic>Bacteria</topic><topic>Biological Products - pharmacology</topic><topic>Care and treatment</topic><topic>Cell Line</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>Disease Models, Animal</topic><topic>Disulfides - pharmacology</topic><topic>DNA</topic><topic>Epithelial Cells</topic><topic>Female</topic><topic>Fish</topic><topic>Gene expression</topic><topic>Gram-positive bacteria</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Infections</topic><topic>Liver</topic><topic>Mice</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Structure</topic><topic>Natural products</topic><topic>psammaplin A</topic><topic>Sepsis</topic><topic>Septicemia</topic><topic>Small intestine</topic><topic>Spleen</topic><topic>Studies</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tyrosine - analogs & derivatives</topic><topic>Tyrosine - pharmacology</topic><topic>Vibrio infections</topic><topic>Vibrio Infections - drug therapy</topic><topic>Vibrio Infections - microbiology</topic><topic>Vibrio Infections - mortality</topic><topic>Vibrio Infections - pathology</topic><topic>Vibrio vulnificus</topic><topic>Vibrio vulnificus - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Byung Cheol</creatorcontrib><creatorcontrib>Lee, Arim</creatorcontrib><creatorcontrib>Jung, Jee Hyung</creatorcontrib><creatorcontrib>Choi, Sang Ho</creatorcontrib><creatorcontrib>Kim, Tae Sung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Byung Cheol</au><au>Lee, Arim</au><au>Jung, Jee Hyung</au><au>Choi, Sang Ho</au><au>Kim, Tae Sung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro and in vivo anti-Vibrio vulnificus activity of psammaplin A, a natural marine compound</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>14</volume><issue>3</issue><spage>2691</spage><epage>2696</epage><pages>2691-2696</pages><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>Vibrio vulnificus is known to induce severely fulminant and fatal septicemia in susceptible hosts. In the present study, the antimicrobial activity of natural marine product-derived compounds against V. vulnificus, were investigated in vitro and in vivo. Twelve pure compounds were isolated from natural marine products and their inhibitory effects on V. vulnificus-induced cytotoxicity were determined in INT-407 cells. Among the 12 pure compounds tested, treatment with psammaplin A significantly suppressed V. vulnificus-induced cytotoxicity in INT-407 cells. Notably, treatment with psammaplin A (5-50 μg) had improved survival rates compared with that in the untreated mice, when the mice were infected with V. vulnificus intraperitoneally. In addition, the bacterial load of V. vulnificus in several tissues (spleen, liver and small intestine) was significantly lower in psammaplin A-treated mice than in untreated mice. Furthermore, psammaplin A treatment significantly suppressed the growth of V. vulnificus. Taken together, these results indicate that psammaplin A may be a potential agent for the prevention and treatment of V. vulnificus infections.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>27431807</pmid><doi>10.3892/mmr.2016.5522</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular medicine reports, 2016-09, Vol.14 (3), p.2691-2696 |
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| source | Spandidos Publications Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Anti-Bacterial Agents - pharmacology Antibiotics Antimicrobial activity Antimicrobial agents Apoptosis Aquatic Organisms - chemistry Bacteria Biological Products - pharmacology Care and treatment Cell Line Cytotoxicity Deoxyribonucleic acid Disease Models, Animal Disulfides - pharmacology DNA Epithelial Cells Female Fish Gene expression Gram-positive bacteria Health aspects Humans Infections Liver Mice Microbial Sensitivity Tests Molecular Structure Natural products psammaplin A Sepsis Septicemia Small intestine Spleen Studies Tumor necrosis factor-TNF Tyrosine - analogs & derivatives Tyrosine - pharmacology Vibrio infections Vibrio Infections - drug therapy Vibrio Infections - microbiology Vibrio Infections - mortality Vibrio Infections - pathology Vibrio vulnificus Vibrio vulnificus - drug effects |
| title | In vitro and in vivo anti-Vibrio vulnificus activity of psammaplin A, a natural marine compound |
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