Expression analysis of serum microRNAs in idiopathic pulmonary fibrosis
Idiopathic pulmonary fibrosis (IPF) is a disease of unknown etiology with considerable morbidity and mortality. Seeking informative diagnostic markers with greater clinical significance is essential for the early diagnosis of IPF. microRNAs (miRNAs or miRs) have emerged as novel serum diagnostic bio...
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| Veröffentlicht in: | International journal of molecular medicine 2014-06, Vol.33 (6), p.1554-1562 |
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| creator | LI, PING LI, JUAN CHEN, TENGFEI WANG, HUAQI CHU, HEYING CHANG, JINGXIA ZANG, WENQIAO WANG, YUANYUAN MA, YUNYUN DU, YUWEN ZHAO, GUOQIANG ZHANG, GUOJUN |
| description | Idiopathic pulmonary fibrosis (IPF) is a disease of unknown etiology with considerable morbidity and mortality. Seeking informative diagnostic markers with greater clinical significance is essential for the early diagnosis of IPF. microRNAs (miRNAs or miRs) have emerged as novel serum diagnostic biomarkers for various diseases. In this study, we performed microarray analysis of the miRNA expression profile in the serum of patients with IPF compared to that of control subjects. We then performed a preliminary analysis of biological functions for the most differentially expressed miRNAs. Some of the microarray results were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The results from this study provide evidence to link the biological role of miRNAs to IPF, and suggest that miRNAs may undertake a variety of functions. Additionally, we found that the altered expression levels of miR-21, miR-155 and miR-101-3p were associated with forced vital capacity (FVC) and radiological features in IPF. Our data may serve as a basis for further investigation, preferably in large prospective studies, before miRNA can be used as a non-invasive screening tool for IPF in routine clinical practice. |
| doi_str_mv | 10.3892/ijmm.2014.1712 |
| format | Article |
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Seeking informative diagnostic markers with greater clinical significance is essential for the early diagnosis of IPF. microRNAs (miRNAs or miRs) have emerged as novel serum diagnostic biomarkers for various diseases. In this study, we performed microarray analysis of the miRNA expression profile in the serum of patients with IPF compared to that of control subjects. We then performed a preliminary analysis of biological functions for the most differentially expressed miRNAs. Some of the microarray results were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The results from this study provide evidence to link the biological role of miRNAs to IPF, and suggest that miRNAs may undertake a variety of functions. Additionally, we found that the altered expression levels of miR-21, miR-155 and miR-101-3p were associated with forced vital capacity (FVC) and radiological features in IPF. Our data may serve as a basis for further investigation, preferably in large prospective studies, before miRNA can be used as a non-invasive screening tool for IPF in routine clinical practice.</description><identifier>ISSN: 1107-3756</identifier><identifier>EISSN: 1791-244X</identifier><identifier>DOI: 10.3892/ijmm.2014.1712</identifier><identifier>PMID: 24676360</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Analysis ; Biomarkers ; Female ; Gene expression ; Gene Expression Profiling ; Genetic aspects ; Humans ; Hybridization ; idiopathic pulmonary fibrosis ; Idiopathic Pulmonary Fibrosis - genetics ; Labeling ; Male ; Medical prognosis ; microarray ; MicroRNA ; MicroRNAs ; MicroRNAs - blood ; Polymerase chain reaction ; Pulmonary fibrosis ; quantitative reverse transcription-polymerase chain reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Risk factors ; serum microRNA ; Software ; Studies ; Tomography ; Vital Capacity - physiology</subject><ispartof>International journal of molecular medicine, 2014-06, Vol.33 (6), p.1554-1562</ispartof><rights>Copyright © 2014, Spandidos Publications</rights><rights>COPYRIGHT 2014 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-25ee2e13e6537de1913c299624199db28c06804758c991ef2cea1b0622292fa63</citedby><cites>FETCH-LOGICAL-c490t-25ee2e13e6537de1913c299624199db28c06804758c991ef2cea1b0622292fa63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,5556,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24676360$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LI, PING</creatorcontrib><creatorcontrib>LI, JUAN</creatorcontrib><creatorcontrib>CHEN, TENGFEI</creatorcontrib><creatorcontrib>WANG, HUAQI</creatorcontrib><creatorcontrib>CHU, HEYING</creatorcontrib><creatorcontrib>CHANG, JINGXIA</creatorcontrib><creatorcontrib>ZANG, WENQIAO</creatorcontrib><creatorcontrib>WANG, YUANYUAN</creatorcontrib><creatorcontrib>MA, YUNYUN</creatorcontrib><creatorcontrib>DU, YUWEN</creatorcontrib><creatorcontrib>ZHAO, GUOQIANG</creatorcontrib><creatorcontrib>ZHANG, GUOJUN</creatorcontrib><title>Expression analysis of serum microRNAs in idiopathic pulmonary fibrosis</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>Idiopathic pulmonary fibrosis (IPF) is a disease of unknown etiology with considerable morbidity and mortality. Seeking informative diagnostic markers with greater clinical significance is essential for the early diagnosis of IPF. microRNAs (miRNAs or miRs) have emerged as novel serum diagnostic biomarkers for various diseases. In this study, we performed microarray analysis of the miRNA expression profile in the serum of patients with IPF compared to that of control subjects. We then performed a preliminary analysis of biological functions for the most differentially expressed miRNAs. Some of the microarray results were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The results from this study provide evidence to link the biological role of miRNAs to IPF, and suggest that miRNAs may undertake a variety of functions. Additionally, we found that the altered expression levels of miR-21, miR-155 and miR-101-3p were associated with forced vital capacity (FVC) and radiological features in IPF. Our data may serve as a basis for further investigation, preferably in large prospective studies, before miRNA can be used as a non-invasive screening tool for IPF in routine clinical practice.</description><subject>Analysis</subject><subject>Biomarkers</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Genetic aspects</subject><subject>Humans</subject><subject>Hybridization</subject><subject>idiopathic pulmonary fibrosis</subject><subject>Idiopathic Pulmonary Fibrosis - genetics</subject><subject>Labeling</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>microarray</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - blood</subject><subject>Polymerase chain reaction</subject><subject>Pulmonary fibrosis</subject><subject>quantitative reverse transcription-polymerase chain reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Risk factors</subject><subject>serum microRNA</subject><subject>Software</subject><subject>Studies</subject><subject>Tomography</subject><subject>Vital Capacity - physiology</subject><issn>1107-3756</issn><issn>1791-244X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpNkMtLxDAQh4Movq8epeDFS9fMJE2a4yK-QBREwVvIpqlm2TY12YL-96asiqeZwzeP30fICdAZqxVe-GXXzZACn4EE3CL7IBWUyPnrdu6BypLJSuyRg5SWlGLFVb1L9pALKZig--Tm6nOILiUf-sL0ZvWVfCpCWyQXx67ovI3h6WGeCt8XvvFhMOt3b4thXHWhN_GraP0ihjxzRHZas0ru-Kcekpfrq-fL2_L-8ebucn5fWq7ousTKOXTAnKiYbBwoYBaVEshBqWaBtaWiplxWtVUKXIvWGVhQgYgKWyPYITnb7B1i-BhdWutlGGN-PGlQDFmVY_2j3szKad-3YR2N7Xyyes6BgaS1pJmabagcMqXoWj1E3-VUGqie7OrJrp7s6sluHjj9OT4uOtf84b86M3C-AdJg-sY3If0x06qSsZKKEqqKs2-XuYFN</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>LI, PING</creator><creator>LI, JUAN</creator><creator>CHEN, TENGFEI</creator><creator>WANG, HUAQI</creator><creator>CHU, HEYING</creator><creator>CHANG, JINGXIA</creator><creator>ZANG, WENQIAO</creator><creator>WANG, YUANYUAN</creator><creator>MA, YUNYUN</creator><creator>DU, YUWEN</creator><creator>ZHAO, GUOQIANG</creator><creator>ZHANG, GUOJUN</creator><general>D.A. 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Seeking informative diagnostic markers with greater clinical significance is essential for the early diagnosis of IPF. microRNAs (miRNAs or miRs) have emerged as novel serum diagnostic biomarkers for various diseases. In this study, we performed microarray analysis of the miRNA expression profile in the serum of patients with IPF compared to that of control subjects. We then performed a preliminary analysis of biological functions for the most differentially expressed miRNAs. Some of the microarray results were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The results from this study provide evidence to link the biological role of miRNAs to IPF, and suggest that miRNAs may undertake a variety of functions. Additionally, we found that the altered expression levels of miR-21, miR-155 and miR-101-3p were associated with forced vital capacity (FVC) and radiological features in IPF. Our data may serve as a basis for further investigation, preferably in large prospective studies, before miRNA can be used as a non-invasive screening tool for IPF in routine clinical practice.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>24676360</pmid><doi>10.3892/ijmm.2014.1712</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1107-3756 |
| ispartof | International journal of molecular medicine, 2014-06, Vol.33 (6), p.1554-1562 |
| issn | 1107-3756 1791-244X |
| language | eng |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Analysis Biomarkers Female Gene expression Gene Expression Profiling Genetic aspects Humans Hybridization idiopathic pulmonary fibrosis Idiopathic Pulmonary Fibrosis - genetics Labeling Male Medical prognosis microarray MicroRNA MicroRNAs MicroRNAs - blood Polymerase chain reaction Pulmonary fibrosis quantitative reverse transcription-polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction Risk factors serum microRNA Software Studies Tomography Vital Capacity - physiology |
| title | Expression analysis of serum microRNAs in idiopathic pulmonary fibrosis |
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