The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis
Long non-coding RNAs (lncRNAs) are emerging as key molecules in human cancer. Homeobox (HOX) transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is associated with a variety of human cancers, such as breast, liver and lung cancer. However, whether HOTAIR can function as a m...
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| Veröffentlicht in: | International journal of molecular medicine 2014-02, Vol.33 (2), p.325-332 |
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| creator | HE, XIAOYING BAO, WEI LI, XIAOCUI CHEN, ZHENG CHE, QI WANG, HUIHUI WAN, XIAO-PING |
| description | Long non-coding RNAs (lncRNAs) are emerging as key molecules in human cancer. Homeobox (HOX) transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is associated with a variety of human cancers, such as breast, liver and lung cancer. However, whether HOTAIR can function as a molecular marker in endometrial carcinoma (EC) remains unknown. In the present study, the expression of HOTAIR in 66 EC tissues from patients with EC and 30 normal tissues from healthy age-matched control subjects was determined using quantitative reverse transcription PCR. Furthermore, using in situ hybridization, we measured HOTAIR expression in 129 formalin-fixed paraffin-embedded (FFPE) tissue sections, which included 96 tissues that matched the frozen cases, 21 other EC tissues and 12 atypical hyperplasia tissues. Correlations between HOTAIR expression and the clinicopathological characteristics of patients were analyzed. Our results revealed that HOTAIR expression in the EC tissues was significantly upregulated compared with normal tissues (p |
| doi_str_mv | 10.3892/ijmm.2013.1570 |
| format | Article |
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Homeobox (HOX) transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is associated with a variety of human cancers, such as breast, liver and lung cancer. However, whether HOTAIR can function as a molecular marker in endometrial carcinoma (EC) remains unknown. In the present study, the expression of HOTAIR in 66 EC tissues from patients with EC and 30 normal tissues from healthy age-matched control subjects was determined using quantitative reverse transcription PCR. Furthermore, using in situ hybridization, we measured HOTAIR expression in 129 formalin-fixed paraffin-embedded (FFPE) tissue sections, which included 96 tissues that matched the frozen cases, 21 other EC tissues and 12 atypical hyperplasia tissues. Correlations between HOTAIR expression and the clinicopathological characteristics of patients were analyzed. Our results revealed that HOTAIR expression in the EC tissues was significantly upregulated compared with normal tissues (p<0.001). In addition, we observed a significant association between HOTAIR expression and the EC grade (p<0.05) and lymph node metastasis (p<0.05). Moreover, in the FFPE tissues, but not the frozen tissues, we found that a higher HOTAIR expression also correlated with the depth of myometrial invasion (p=0.019) and lymphovascular space invasion (p=0.015). More importantly, patients with a higher HOTAIR expression showed significantly poorer overall survival than those with lower HOTAIR expression (p<0.05). In conclusion, our results suggest that a high expression of HOTAIR is involved in the progression of cancer and may be a novel biomarker of poor prognosis in patients with EC.</description><identifier>ISSN: 1107-3756</identifier><identifier>EISSN: 1791-244X</identifier><identifier>DOI: 10.3892/ijmm.2013.1570</identifier><identifier>PMID: 24285342</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Apoptosis ; Cancer therapies ; Care and treatment ; Childrens health ; Diagnosis ; Disease Progression ; Endometrial cancer ; endometrial carcinoma ; Endometrial Neoplasms - diagnosis ; Endometrial Neoplasms - genetics ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Gynecology ; homeobox (HOX) transcript antisense intergenic RNA ; Humans ; Hybridization ; Immunohistochemistry ; long non-coding RNA ; Lymphatic Metastasis - diagnosis ; Lymphatic Metastasis - genetics ; Medical prognosis ; Metastasis ; Middle Aged ; Obstetrics ; Prognosis ; Risk factors ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; Studies ; Tumor markers ; Tumorigenesis ; Tumors ; Up-Regulation</subject><ispartof>International journal of molecular medicine, 2014-02, Vol.33 (2), p.325-332</ispartof><rights>Copyright © 2014, Spandidos Publications</rights><rights>COPYRIGHT 2014 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-83eb5b19dae9f57129b9d7cce6f5157b4232a8637119504b3da0ae4b1d82f9b03</citedby><cites>FETCH-LOGICAL-c463t-83eb5b19dae9f57129b9d7cce6f5157b4232a8637119504b3da0ae4b1d82f9b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,5558,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24285342$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HE, XIAOYING</creatorcontrib><creatorcontrib>BAO, WEI</creatorcontrib><creatorcontrib>LI, XIAOCUI</creatorcontrib><creatorcontrib>CHEN, ZHENG</creatorcontrib><creatorcontrib>CHE, QI</creatorcontrib><creatorcontrib>WANG, HUIHUI</creatorcontrib><creatorcontrib>WAN, XIAO-PING</creatorcontrib><title>The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>Long non-coding RNAs (lncRNAs) are emerging as key molecules in human cancer. Homeobox (HOX) transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is associated with a variety of human cancers, such as breast, liver and lung cancer. However, whether HOTAIR can function as a molecular marker in endometrial carcinoma (EC) remains unknown. In the present study, the expression of HOTAIR in 66 EC tissues from patients with EC and 30 normal tissues from healthy age-matched control subjects was determined using quantitative reverse transcription PCR. Furthermore, using in situ hybridization, we measured HOTAIR expression in 129 formalin-fixed paraffin-embedded (FFPE) tissue sections, which included 96 tissues that matched the frozen cases, 21 other EC tissues and 12 atypical hyperplasia tissues. Correlations between HOTAIR expression and the clinicopathological characteristics of patients were analyzed. Our results revealed that HOTAIR expression in the EC tissues was significantly upregulated compared with normal tissues (p<0.001). In addition, we observed a significant association between HOTAIR expression and the EC grade (p<0.05) and lymph node metastasis (p<0.05). Moreover, in the FFPE tissues, but not the frozen tissues, we found that a higher HOTAIR expression also correlated with the depth of myometrial invasion (p=0.019) and lymphovascular space invasion (p=0.015). More importantly, patients with a higher HOTAIR expression showed significantly poorer overall survival than those with lower HOTAIR expression (p<0.05). In conclusion, our results suggest that a high expression of HOTAIR is involved in the progression of cancer and may be a novel biomarker of poor prognosis in patients with EC.</description><subject>Apoptosis</subject><subject>Cancer therapies</subject><subject>Care and treatment</subject><subject>Childrens health</subject><subject>Diagnosis</subject><subject>Disease Progression</subject><subject>Endometrial cancer</subject><subject>endometrial carcinoma</subject><subject>Endometrial Neoplasms - diagnosis</subject><subject>Endometrial Neoplasms - genetics</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Gynecology</subject><subject>homeobox (HOX) transcript antisense intergenic RNA</subject><subject>Humans</subject><subject>Hybridization</subject><subject>Immunohistochemistry</subject><subject>long non-coding RNA</subject><subject>Lymphatic Metastasis - diagnosis</subject><subject>Lymphatic Metastasis - genetics</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>Obstetrics</subject><subject>Prognosis</subject><subject>Risk factors</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Studies</subject><subject>Tumor markers</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><subject>Up-Regulation</subject><issn>1107-3756</issn><issn>1791-244X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptkc9rHCEUgCW0NGnaa45F6KUXt-rTnfG4hLYJhAbCFnoTR52Ny4xOdYaS_z4OSdNL8OBDvvfD9yF0wegGWsW_huM4bjhlsGGyoSfojDWKES7E7zc1ZrQh0MjtKXpfypFSLoVq36FTLngrQfAzFPb3Hg8pHnBMkdjkQg3vfu7w1e1-d32HQ8HLlP1hGczsHQ4R--jS6OcczICtyTbENBpsosM25exXruC_Yb7HU0oZTzkdYiqhfEBvezMU__H5Pke_vn_bX16Rm9sf15e7G2LFFmbSgu9kx5QzXvWyYVx1yjXW-m0v6xc7wYGbdgsNY0pS0YEz1HjRMdfyXnUUztHnp7q185_Fl1kf05JjbamZAg5StCD_UwczeB1in-Zs7BiK1TvBJEhVd1SpzStUPc6Pwabo-1DfX0uwOZWSfa-nHEaTHzSjehWmV2F6FaZXYTXh0_O0Szd694L_M1SBL09AmeqOg0vlhVlLEQBCOaHAJTwC2tmdVg</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>HE, XIAOYING</creator><creator>BAO, WEI</creator><creator>LI, XIAOCUI</creator><creator>CHEN, ZHENG</creator><creator>CHE, QI</creator><creator>WANG, HUIHUI</creator><creator>WAN, XIAO-PING</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20140201</creationdate><title>The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis</title><author>HE, XIAOYING ; BAO, WEI ; LI, XIAOCUI ; CHEN, ZHENG ; CHE, QI ; WANG, HUIHUI ; WAN, XIAO-PING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-83eb5b19dae9f57129b9d7cce6f5157b4232a8637119504b3da0ae4b1d82f9b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Apoptosis</topic><topic>Cancer therapies</topic><topic>Care and treatment</topic><topic>Childrens health</topic><topic>Diagnosis</topic><topic>Disease Progression</topic><topic>Endometrial cancer</topic><topic>endometrial carcinoma</topic><topic>Endometrial Neoplasms - diagnosis</topic><topic>Endometrial Neoplasms - genetics</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic aspects</topic><topic>Gynecology</topic><topic>homeobox (HOX) transcript antisense intergenic RNA</topic><topic>Humans</topic><topic>Hybridization</topic><topic>Immunohistochemistry</topic><topic>long non-coding RNA</topic><topic>Lymphatic Metastasis - diagnosis</topic><topic>Lymphatic Metastasis - genetics</topic><topic>Medical prognosis</topic><topic>Metastasis</topic><topic>Middle Aged</topic><topic>Obstetrics</topic><topic>Prognosis</topic><topic>Risk factors</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Studies</topic><topic>Tumor markers</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HE, XIAOYING</creatorcontrib><creatorcontrib>BAO, WEI</creatorcontrib><creatorcontrib>LI, XIAOCUI</creatorcontrib><creatorcontrib>CHEN, ZHENG</creatorcontrib><creatorcontrib>CHE, QI</creatorcontrib><creatorcontrib>WANG, HUIHUI</creatorcontrib><creatorcontrib>WAN, XIAO-PING</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>International journal of molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HE, XIAOYING</au><au>BAO, WEI</au><au>LI, XIAOCUI</au><au>CHEN, ZHENG</au><au>CHE, QI</au><au>WANG, HUIHUI</au><au>WAN, XIAO-PING</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis</atitle><jtitle>International journal of molecular medicine</jtitle><addtitle>Int J Mol Med</addtitle><date>2014-02-01</date><risdate>2014</risdate><volume>33</volume><issue>2</issue><spage>325</spage><epage>332</epage><pages>325-332</pages><issn>1107-3756</issn><eissn>1791-244X</eissn><abstract>Long non-coding RNAs (lncRNAs) are emerging as key molecules in human cancer. Homeobox (HOX) transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is associated with a variety of human cancers, such as breast, liver and lung cancer. However, whether HOTAIR can function as a molecular marker in endometrial carcinoma (EC) remains unknown. In the present study, the expression of HOTAIR in 66 EC tissues from patients with EC and 30 normal tissues from healthy age-matched control subjects was determined using quantitative reverse transcription PCR. Furthermore, using in situ hybridization, we measured HOTAIR expression in 129 formalin-fixed paraffin-embedded (FFPE) tissue sections, which included 96 tissues that matched the frozen cases, 21 other EC tissues and 12 atypical hyperplasia tissues. Correlations between HOTAIR expression and the clinicopathological characteristics of patients were analyzed. Our results revealed that HOTAIR expression in the EC tissues was significantly upregulated compared with normal tissues (p<0.001). In addition, we observed a significant association between HOTAIR expression and the EC grade (p<0.05) and lymph node metastasis (p<0.05). Moreover, in the FFPE tissues, but not the frozen tissues, we found that a higher HOTAIR expression also correlated with the depth of myometrial invasion (p=0.019) and lymphovascular space invasion (p=0.015). More importantly, patients with a higher HOTAIR expression showed significantly poorer overall survival than those with lower HOTAIR expression (p<0.05). In conclusion, our results suggest that a high expression of HOTAIR is involved in the progression of cancer and may be a novel biomarker of poor prognosis in patients with EC.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>24285342</pmid><doi>10.3892/ijmm.2013.1570</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Apoptosis Cancer therapies Care and treatment Childrens health Diagnosis Disease Progression Endometrial cancer endometrial carcinoma Endometrial Neoplasms - diagnosis Endometrial Neoplasms - genetics Female Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Gynecology homeobox (HOX) transcript antisense intergenic RNA Humans Hybridization Immunohistochemistry long non-coding RNA Lymphatic Metastasis - diagnosis Lymphatic Metastasis - genetics Medical prognosis Metastasis Middle Aged Obstetrics Prognosis Risk factors RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Studies Tumor markers Tumorigenesis Tumors Up-Regulation |
| title | The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis |
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