Vigna angularis inhibits mast cell-mediated allergic inflammation

The aim of the present study was to elucidate whether extracts of Vigna angularis (EVA) inhibit allergic inflammatory reactions and to elucidate the possible mechanisms of action. For the assessment of allergic inflammatory response, histamine release and the expression of pro-inflammatory cytokines...

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Veröffentlicht in:International journal of molecular medicine 2013-09, Vol.32 (3), p.736-742
Hauptverfasser: KIM, HUI-HUN, KIM, SUNG-WAN, KIM, DUK-SIL, OH, HYUN-MEE, RHO, MUN-CHUAL, KIM, SANG-HYUN
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container_issue 3
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container_title International journal of molecular medicine
container_volume 32
creator KIM, HUI-HUN
KIM, SUNG-WAN
KIM, DUK-SIL
OH, HYUN-MEE
RHO, MUN-CHUAL
KIM, SANG-HYUN
description The aim of the present study was to elucidate whether extracts of Vigna angularis (EVA) inhibit allergic inflammatory reactions and to elucidate the possible mechanisms of action. For the assessment of allergic inflammatory response, histamine release and the expression of pro-inflammatory cytokines from human mast cells (HMC-1) were examined. To identify the underlying mechanisms of action, intracellular calcium and the activation of nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) were assayed. To confirm the effects of EVA in vivo, systemic and local allergic reaction mouse models were employed. EVA dose-dependently reduced phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-induced histamine release from mast cells. The inhibitory effects of EVA on the release of histamine from mast cells were mediated by the reduction of intracellular calcium levels. EVA decreased the PMACI-stimulated gene expression and secretion of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6. The inhibitory effects of EVA on pro-inflammatory cytokines were NF-κB- and MAPK-dependent. In addition, EVA inhibited compound 48/80-induced systemic anaphylaxis and immunoglobulin E (IgE)-mediated cutaneous anaphylaxis. Our findings provide evidence that EVA inhibits mast cell-derived allergic inflammation, and suggest the possible therapeutic application of EVA in allergic inflammatory disorders.
doi_str_mv 10.3892/ijmm.2013.1430
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For the assessment of allergic inflammatory response, histamine release and the expression of pro-inflammatory cytokines from human mast cells (HMC-1) were examined. To identify the underlying mechanisms of action, intracellular calcium and the activation of nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) were assayed. To confirm the effects of EVA in vivo, systemic and local allergic reaction mouse models were employed. EVA dose-dependently reduced phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-induced histamine release from mast cells. The inhibitory effects of EVA on the release of histamine from mast cells were mediated by the reduction of intracellular calcium levels. EVA decreased the PMACI-stimulated gene expression and secretion of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6. The inhibitory effects of EVA on pro-inflammatory cytokines were NF-κB- and MAPK-dependent. 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Spandidos</publisher><subject>allergic inflammation ; Anaphylaxis ; Anaphylaxis - drug therapy ; Anaphylaxis - immunology ; Anaphylaxis - metabolism ; Animals ; Anti-Allergic Agents - administration &amp; dosage ; Anti-Allergic Agents - pharmacology ; Calcium - metabolism ; Cell Line ; Cytokines ; Cytokines - biosynthesis ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Enzymes ; Fabaceae - chemistry ; Gene expression ; Histamine ; Histamine Release - drug effects ; Histamine Release - immunology ; Humans ; Hypersensitivity - drug therapy ; Hypersensitivity - immunology ; Hypersensitivity - metabolism ; Inflammation ; Inflammation Mediators - metabolism ; Kinases ; Male ; mast cells ; Mast Cells - drug effects ; Mast Cells - immunology ; Mast Cells - metabolism ; Mice ; Mitogen-Activated Protein Kinases - metabolism ; NF-kappa B - metabolism ; Phosphorylation ; Plant Extracts - administration &amp; dosage ; Plant Extracts - genetics ; Plant Extracts - pharmacology ; pro-inflammatory cytokine ; Proteins ; Rodents ; Signal Transduction - drug effects ; Tumor necrosis factor-TNF ; Vigna angularis</subject><ispartof>International journal of molecular medicine, 2013-09, Vol.32 (3), p.736-742</ispartof><rights>Copyright © 2013, Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-7c9f47cde22c7d40c5e6ff2de2c3c4f30a2c2e794d44ec6fc6667bb8e4f5ae2c3</citedby><cites>FETCH-LOGICAL-c396t-7c9f47cde22c7d40c5e6ff2de2c3c4f30a2c2e794d44ec6fc6667bb8e4f5ae2c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,5571,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23828310$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KIM, HUI-HUN</creatorcontrib><creatorcontrib>KIM, SUNG-WAN</creatorcontrib><creatorcontrib>KIM, DUK-SIL</creatorcontrib><creatorcontrib>OH, HYUN-MEE</creatorcontrib><creatorcontrib>RHO, MUN-CHUAL</creatorcontrib><creatorcontrib>KIM, SANG-HYUN</creatorcontrib><title>Vigna angularis inhibits mast cell-mediated allergic inflammation</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>The aim of the present study was to elucidate whether extracts of Vigna angularis (EVA) inhibit allergic inflammatory reactions and to elucidate the possible mechanisms of action. For the assessment of allergic inflammatory response, histamine release and the expression of pro-inflammatory cytokines from human mast cells (HMC-1) were examined. To identify the underlying mechanisms of action, intracellular calcium and the activation of nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) were assayed. To confirm the effects of EVA in vivo, systemic and local allergic reaction mouse models were employed. EVA dose-dependently reduced phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-induced histamine release from mast cells. The inhibitory effects of EVA on the release of histamine from mast cells were mediated by the reduction of intracellular calcium levels. EVA decreased the PMACI-stimulated gene expression and secretion of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6. The inhibitory effects of EVA on pro-inflammatory cytokines were NF-κB- and MAPK-dependent. In addition, EVA inhibited compound 48/80-induced systemic anaphylaxis and immunoglobulin E (IgE)-mediated cutaneous anaphylaxis. 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In addition, EVA inhibited compound 48/80-induced systemic anaphylaxis and immunoglobulin E (IgE)-mediated cutaneous anaphylaxis. Our findings provide evidence that EVA inhibits mast cell-derived allergic inflammation, and suggest the possible therapeutic application of EVA in allergic inflammatory disorders.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>23828310</pmid><doi>10.3892/ijmm.2013.1430</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
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source Spandidos Publications Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects allergic inflammation
Anaphylaxis
Anaphylaxis - drug therapy
Anaphylaxis - immunology
Anaphylaxis - metabolism
Animals
Anti-Allergic Agents - administration & dosage
Anti-Allergic Agents - pharmacology
Calcium - metabolism
Cell Line
Cytokines
Cytokines - biosynthesis
Disease Models, Animal
Dose-Response Relationship, Drug
Enzymes
Fabaceae - chemistry
Gene expression
Histamine
Histamine Release - drug effects
Histamine Release - immunology
Humans
Hypersensitivity - drug therapy
Hypersensitivity - immunology
Hypersensitivity - metabolism
Inflammation
Inflammation Mediators - metabolism
Kinases
Male
mast cells
Mast Cells - drug effects
Mast Cells - immunology
Mast Cells - metabolism
Mice
Mitogen-Activated Protein Kinases - metabolism
NF-kappa B - metabolism
Phosphorylation
Plant Extracts - administration & dosage
Plant Extracts - genetics
Plant Extracts - pharmacology
pro-inflammatory cytokine
Proteins
Rodents
Signal Transduction - drug effects
Tumor necrosis factor-TNF
Vigna angularis
title Vigna angularis inhibits mast cell-mediated allergic inflammation
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