Identification of novel molecular targets regulated by tumor suppressive miR-375 induced by histone acetylation in esophageal squamous cell carcinoma
The aim of this study was to determine whether histone acetylation regulates tumor suppressive microRNAs (miRNAs) in esophageal squamous cell carcinoma (ESCC) and to identify genes which are regulated by these miRNAs. We identified a miRNA that was highly upregulated in an ESCC cell line by cyclic h...
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| Veröffentlicht in: | International journal of oncology 2012-09, Vol.41 (3), p.985-994 |
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| creator | ISOZAKI, YUKA HOSHINO, ISAMU NOHATA, NIJIRO KINOSHITA, TAKASHI AKUTSU, YASUNORI HANARI, NAOYUKI MORI, MIKITO YONEYAMA, YASUO AKANUMA, NAOKI TAKESHITA, NOBUYOSHI MARUYAMA, TETSURO SEKI, NAOHIKO NISHINO, NORIKAZU YOSHIDA, MINORU MATSUBARA, HISAHIRO |
| description | The aim of this study was to determine whether histone acetylation regulates tumor suppressive microRNAs (miRNAs) in esophageal squamous cell carcinoma (ESCC) and to identify genes which are regulated by these miRNAs. We identified a miRNA that was highly upregulated in an ESCC cell line by cyclic hydroxamic acid-containing peptide 31 (CHAP31), one of the histone deacetylase inhibitors (HDACIs), using a miRNA array analysis. miR-375 was strongly upregulated by CHAP31 treatment in an ESCC cell line. The expression levels of the most upregulated miRNA, miR-375 were analyzed by quantitative real-time PCR in human ESCC specimens. The tumor suppressive function of miR-375 was revealed by restoration of miR-375 in ESCC cell lines. We performed a microarray analysis to identify target genes of miR-375. The mRNA and protein expression levels of these genes were verified in ESCC clinical specimens. LDHB and AEG-1/MTDH were detected as miR-375-targeted genes. The restoration of miR-375 suppressed the expression of LDHB and AEG-1/MTDH. The ESCC clinical specimens exhibited a high level of LDHB expression at both the mRNA and protein levels. A loss-of-function assay using a siRNA analysis was performed to examine the oncogenic function of the gene. Knockdown of LDHB by RNAi showed a tumor suppressive function in the ESCC cells. The correlation between gene expression and clinicopathological features was investigated by immunohistochemistry for 94 cases of ESCC. The positive staining of LDHB correlated significantly with lymph node metastasis and tumor stage. It also had a tendency to be associated with a poor prognosis. Our results indicate that HDACIs upregulate miRNAs, at least some of which act as tumor suppressors. LDHB, which is regulated by the tumor suppressive miR-375, may therefore act as an oncogene in ESCC. |
| doi_str_mv | 10.3892/ijo.2012.1537 |
| format | Article |
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We identified a miRNA that was highly upregulated in an ESCC cell line by cyclic hydroxamic acid-containing peptide 31 (CHAP31), one of the histone deacetylase inhibitors (HDACIs), using a miRNA array analysis. miR-375 was strongly upregulated by CHAP31 treatment in an ESCC cell line. The expression levels of the most upregulated miRNA, miR-375 were analyzed by quantitative real-time PCR in human ESCC specimens. The tumor suppressive function of miR-375 was revealed by restoration of miR-375 in ESCC cell lines. We performed a microarray analysis to identify target genes of miR-375. The mRNA and protein expression levels of these genes were verified in ESCC clinical specimens. LDHB and AEG-1/MTDH were detected as miR-375-targeted genes. The restoration of miR-375 suppressed the expression of LDHB and AEG-1/MTDH. The ESCC clinical specimens exhibited a high level of LDHB expression at both the mRNA and protein levels. A loss-of-function assay using a siRNA analysis was performed to examine the oncogenic function of the gene. Knockdown of LDHB by RNAi showed a tumor suppressive function in the ESCC cells. The correlation between gene expression and clinicopathological features was investigated by immunohistochemistry for 94 cases of ESCC. The positive staining of LDHB correlated significantly with lymph node metastasis and tumor stage. It also had a tendency to be associated with a poor prognosis. Our results indicate that HDACIs upregulate miRNAs, at least some of which act as tumor suppressors. LDHB, which is regulated by the tumor suppressive miR-375, may therefore act as an oncogene in ESCC.</description><identifier>ISSN: 1019-6439</identifier><identifier>EISSN: 1791-2423</identifier><identifier>DOI: 10.3892/ijo.2012.1537</identifier><identifier>PMID: 22752059</identifier><language>eng</language><publisher>Athens: D.A. Spandidos</publisher><subject>Acetylation ; AEG-1/MTDH ; Aged ; Apoptosis ; Biological and medical sciences ; Biomarkers, Tumor - genetics ; Cancer therapies ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - metabolism ; Cell Adhesion Molecules - metabolism ; Cell cycle ; Cell growth ; Cell Movement ; Cell Proliferation ; CHAP31 ; Chemotherapy ; DNA methylation ; Epigenetics ; Esophageal cancer ; Esophageal Neoplasms - genetics ; Esophageal Neoplasms - metabolism ; esophageal squamous cell carcinoma ; Esophagus ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Gene expression ; Gene Expression Regulation, Neoplastic ; HDAC inhibitor ; histone acetylation ; Histone Deacetylase Inhibitors - metabolism ; Histones - metabolism ; Humans ; Isoenzymes - metabolism ; L-Lactate Dehydrogenase - metabolism ; LDHB ; Male ; Medical prognosis ; Medical sciences ; Metastasis ; microRNA ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; miR-375 ; Multivariate analysis ; Oligonucleotide Array Sequence Analysis ; Peptides, Cyclic - metabolism ; Radiation therapy ; RNA Interference ; RNA, Small Interfering ; Squamous cell carcinoma ; Studies ; Tumors</subject><ispartof>International journal of oncology, 2012-09, Vol.41 (3), p.985-994</ispartof><rights>Copyright © 2012, Spandidos Publications</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Spandidos Publications UK Ltd. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-e08466060c73ff92385ef0cbf314573177d300eed1b4a52d43ed88ef4c8c62903</citedby><cites>FETCH-LOGICAL-c488t-e08466060c73ff92385ef0cbf314573177d300eed1b4a52d43ed88ef4c8c62903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,5556,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26176110$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22752059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ISOZAKI, YUKA</creatorcontrib><creatorcontrib>HOSHINO, ISAMU</creatorcontrib><creatorcontrib>NOHATA, NIJIRO</creatorcontrib><creatorcontrib>KINOSHITA, TAKASHI</creatorcontrib><creatorcontrib>AKUTSU, YASUNORI</creatorcontrib><creatorcontrib>HANARI, NAOYUKI</creatorcontrib><creatorcontrib>MORI, MIKITO</creatorcontrib><creatorcontrib>YONEYAMA, YASUO</creatorcontrib><creatorcontrib>AKANUMA, NAOKI</creatorcontrib><creatorcontrib>TAKESHITA, NOBUYOSHI</creatorcontrib><creatorcontrib>MARUYAMA, TETSURO</creatorcontrib><creatorcontrib>SEKI, NAOHIKO</creatorcontrib><creatorcontrib>NISHINO, NORIKAZU</creatorcontrib><creatorcontrib>YOSHIDA, MINORU</creatorcontrib><creatorcontrib>MATSUBARA, HISAHIRO</creatorcontrib><title>Identification of novel molecular targets regulated by tumor suppressive miR-375 induced by histone acetylation in esophageal squamous cell carcinoma</title><title>International journal of oncology</title><addtitle>Int J Oncol</addtitle><description>The aim of this study was to determine whether histone acetylation regulates tumor suppressive microRNAs (miRNAs) in esophageal squamous cell carcinoma (ESCC) and to identify genes which are regulated by these miRNAs. We identified a miRNA that was highly upregulated in an ESCC cell line by cyclic hydroxamic acid-containing peptide 31 (CHAP31), one of the histone deacetylase inhibitors (HDACIs), using a miRNA array analysis. miR-375 was strongly upregulated by CHAP31 treatment in an ESCC cell line. The expression levels of the most upregulated miRNA, miR-375 were analyzed by quantitative real-time PCR in human ESCC specimens. The tumor suppressive function of miR-375 was revealed by restoration of miR-375 in ESCC cell lines. We performed a microarray analysis to identify target genes of miR-375. The mRNA and protein expression levels of these genes were verified in ESCC clinical specimens. LDHB and AEG-1/MTDH were detected as miR-375-targeted genes. The restoration of miR-375 suppressed the expression of LDHB and AEG-1/MTDH. The ESCC clinical specimens exhibited a high level of LDHB expression at both the mRNA and protein levels. A loss-of-function assay using a siRNA analysis was performed to examine the oncogenic function of the gene. Knockdown of LDHB by RNAi showed a tumor suppressive function in the ESCC cells. The correlation between gene expression and clinicopathological features was investigated by immunohistochemistry for 94 cases of ESCC. The positive staining of LDHB correlated significantly with lymph node metastasis and tumor stage. It also had a tendency to be associated with a poor prognosis. Our results indicate that HDACIs upregulate miRNAs, at least some of which act as tumor suppressors. LDHB, which is regulated by the tumor suppressive miR-375, may therefore act as an oncogene in ESCC.</description><subject>Acetylation</subject><subject>AEG-1/MTDH</subject><subject>Aged</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Cancer therapies</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>CHAP31</subject><subject>Chemotherapy</subject><subject>DNA methylation</subject><subject>Epigenetics</subject><subject>Esophageal cancer</subject><subject>Esophageal Neoplasms - genetics</subject><subject>Esophageal Neoplasms - metabolism</subject><subject>esophageal squamous cell carcinoma</subject><subject>Esophagus</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>HDAC inhibitor</subject><subject>histone acetylation</subject><subject>Histone Deacetylase Inhibitors - metabolism</subject><subject>Histones - metabolism</subject><subject>Humans</subject><subject>Isoenzymes - metabolism</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>LDHB</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Medical sciences</subject><subject>Metastasis</subject><subject>microRNA</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>miR-375</subject><subject>Multivariate analysis</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Peptides, Cyclic - metabolism</subject><subject>Radiation therapy</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering</subject><subject>Squamous cell carcinoma</subject><subject>Studies</subject><subject>Tumors</subject><issn>1019-6439</issn><issn>1791-2423</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpF0ctq3DAUBmATWnJrl90WQSlZaaqrZS1LaNJAoFDatdFIRxMNtuRIcmAepO9bD542K0nwcX5x_qb5QMmGd5p9Cfu0YYSyDZVcnTWXVGmKmWD8zXInVONWcH3RXJWyJ4RJSeh5c8GYkoxIfdn8eXAQa_DBmhpSRMmjmF5gQGMawM6DyaiavINaUIbd8q7g0PaA6jymjMo8TRlKCS-AxvATcyVRiG62K3oKpaYIyFioh2ENCBFBSdOT2YEZUHmezZjmgiwMA7Im2xDTaN41b70ZCrw_ndfN77tvv26_48cf9w-3Xx-xFV1XMZBOtC1piVXce814J8ETu_WcCqk4VcpxQgAc3QojmRMcXNeBF7azLdOEXzef1rlTTs8zlNrv05zjEtlTzRnnWlC1KLwqm1MpGXw_5TCafOgp6Y8l9EsJ_bGE_ljC4j-eps7bEdx__W_rC_h8AqZYM_hsog3l1bVUtZQev3ezujKZ6IJLr2ZJxIJiwjHRneR_AfTLnvs</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>ISOZAKI, YUKA</creator><creator>HOSHINO, ISAMU</creator><creator>NOHATA, NIJIRO</creator><creator>KINOSHITA, TAKASHI</creator><creator>AKUTSU, YASUNORI</creator><creator>HANARI, NAOYUKI</creator><creator>MORI, MIKITO</creator><creator>YONEYAMA, YASUO</creator><creator>AKANUMA, NAOKI</creator><creator>TAKESHITA, NOBUYOSHI</creator><creator>MARUYAMA, TETSURO</creator><creator>SEKI, NAOHIKO</creator><creator>NISHINO, NORIKAZU</creator><creator>YOSHIDA, MINORU</creator><creator>MATSUBARA, HISAHIRO</creator><general>D.A. Spandidos</general><general>Editorial Academy of the International Journal of Oncology</general><general>Spandidos Publications UK Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20120901</creationdate><title>Identification of novel molecular targets regulated by tumor suppressive miR-375 induced by histone acetylation in esophageal squamous cell carcinoma</title><author>ISOZAKI, YUKA ; HOSHINO, ISAMU ; NOHATA, NIJIRO ; KINOSHITA, TAKASHI ; AKUTSU, YASUNORI ; HANARI, NAOYUKI ; MORI, MIKITO ; YONEYAMA, YASUO ; AKANUMA, NAOKI ; TAKESHITA, NOBUYOSHI ; MARUYAMA, TETSURO ; SEKI, NAOHIKO ; NISHINO, NORIKAZU ; YOSHIDA, MINORU ; MATSUBARA, HISAHIRO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-e08466060c73ff92385ef0cbf314573177d300eed1b4a52d43ed88ef4c8c62903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acetylation</topic><topic>AEG-1/MTDH</topic><topic>Aged</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Cancer therapies</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>CHAP31</topic><topic>Chemotherapy</topic><topic>DNA methylation</topic><topic>Epigenetics</topic><topic>Esophageal cancer</topic><topic>Esophageal Neoplasms - genetics</topic><topic>Esophageal Neoplasms - metabolism</topic><topic>esophageal squamous cell carcinoma</topic><topic>Esophagus</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>HDAC inhibitor</topic><topic>histone acetylation</topic><topic>Histone Deacetylase Inhibitors - metabolism</topic><topic>Histones - metabolism</topic><topic>Humans</topic><topic>Isoenzymes - metabolism</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>LDHB</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>Medical sciences</topic><topic>Metastasis</topic><topic>microRNA</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>miR-375</topic><topic>Multivariate analysis</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Peptides, Cyclic - metabolism</topic><topic>Radiation therapy</topic><topic>RNA Interference</topic><topic>RNA, Small Interfering</topic><topic>Squamous cell carcinoma</topic><topic>Studies</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ISOZAKI, YUKA</creatorcontrib><creatorcontrib>HOSHINO, ISAMU</creatorcontrib><creatorcontrib>NOHATA, NIJIRO</creatorcontrib><creatorcontrib>KINOSHITA, TAKASHI</creatorcontrib><creatorcontrib>AKUTSU, YASUNORI</creatorcontrib><creatorcontrib>HANARI, NAOYUKI</creatorcontrib><creatorcontrib>MORI, MIKITO</creatorcontrib><creatorcontrib>YONEYAMA, YASUO</creatorcontrib><creatorcontrib>AKANUMA, NAOKI</creatorcontrib><creatorcontrib>TAKESHITA, NOBUYOSHI</creatorcontrib><creatorcontrib>MARUYAMA, TETSURO</creatorcontrib><creatorcontrib>SEKI, NAOHIKO</creatorcontrib><creatorcontrib>NISHINO, NORIKAZU</creatorcontrib><creatorcontrib>YOSHIDA, MINORU</creatorcontrib><creatorcontrib>MATSUBARA, HISAHIRO</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>International journal of oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ISOZAKI, YUKA</au><au>HOSHINO, ISAMU</au><au>NOHATA, NIJIRO</au><au>KINOSHITA, TAKASHI</au><au>AKUTSU, YASUNORI</au><au>HANARI, NAOYUKI</au><au>MORI, MIKITO</au><au>YONEYAMA, YASUO</au><au>AKANUMA, NAOKI</au><au>TAKESHITA, NOBUYOSHI</au><au>MARUYAMA, TETSURO</au><au>SEKI, NAOHIKO</au><au>NISHINO, NORIKAZU</au><au>YOSHIDA, MINORU</au><au>MATSUBARA, HISAHIRO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of novel molecular targets regulated by tumor suppressive miR-375 induced by histone acetylation in esophageal squamous cell carcinoma</atitle><jtitle>International journal of oncology</jtitle><addtitle>Int J Oncol</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>41</volume><issue>3</issue><spage>985</spage><epage>994</epage><pages>985-994</pages><issn>1019-6439</issn><eissn>1791-2423</eissn><abstract>The aim of this study was to determine whether histone acetylation regulates tumor suppressive microRNAs (miRNAs) in esophageal squamous cell carcinoma (ESCC) and to identify genes which are regulated by these miRNAs. We identified a miRNA that was highly upregulated in an ESCC cell line by cyclic hydroxamic acid-containing peptide 31 (CHAP31), one of the histone deacetylase inhibitors (HDACIs), using a miRNA array analysis. miR-375 was strongly upregulated by CHAP31 treatment in an ESCC cell line. The expression levels of the most upregulated miRNA, miR-375 were analyzed by quantitative real-time PCR in human ESCC specimens. The tumor suppressive function of miR-375 was revealed by restoration of miR-375 in ESCC cell lines. We performed a microarray analysis to identify target genes of miR-375. The mRNA and protein expression levels of these genes were verified in ESCC clinical specimens. LDHB and AEG-1/MTDH were detected as miR-375-targeted genes. The restoration of miR-375 suppressed the expression of LDHB and AEG-1/MTDH. The ESCC clinical specimens exhibited a high level of LDHB expression at both the mRNA and protein levels. A loss-of-function assay using a siRNA analysis was performed to examine the oncogenic function of the gene. Knockdown of LDHB by RNAi showed a tumor suppressive function in the ESCC cells. The correlation between gene expression and clinicopathological features was investigated by immunohistochemistry for 94 cases of ESCC. The positive staining of LDHB correlated significantly with lymph node metastasis and tumor stage. It also had a tendency to be associated with a poor prognosis. Our results indicate that HDACIs upregulate miRNAs, at least some of which act as tumor suppressors. LDHB, which is regulated by the tumor suppressive miR-375, may therefore act as an oncogene in ESCC.</abstract><cop>Athens</cop><pub>D.A. Spandidos</pub><pmid>22752059</pmid><doi>10.3892/ijo.2012.1537</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1019-6439 |
| ispartof | International journal of oncology, 2012-09, Vol.41 (3), p.985-994 |
| issn | 1019-6439 1791-2423 |
| language | eng |
| recordid | cdi_proquest_journals_1932339417 |
| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Acetylation AEG-1/MTDH Aged Apoptosis Biological and medical sciences Biomarkers, Tumor - genetics Cancer therapies Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - metabolism Cell Adhesion Molecules - metabolism Cell cycle Cell growth Cell Movement Cell Proliferation CHAP31 Chemotherapy DNA methylation Epigenetics Esophageal cancer Esophageal Neoplasms - genetics Esophageal Neoplasms - metabolism esophageal squamous cell carcinoma Esophagus Female Gastroenterology. Liver. Pancreas. Abdomen Gene expression Gene Expression Regulation, Neoplastic HDAC inhibitor histone acetylation Histone Deacetylase Inhibitors - metabolism Histones - metabolism Humans Isoenzymes - metabolism L-Lactate Dehydrogenase - metabolism LDHB Male Medical prognosis Medical sciences Metastasis microRNA MicroRNAs - genetics MicroRNAs - metabolism Middle Aged miR-375 Multivariate analysis Oligonucleotide Array Sequence Analysis Peptides, Cyclic - metabolism Radiation therapy RNA Interference RNA, Small Interfering Squamous cell carcinoma Studies Tumors |
| title | Identification of novel molecular targets regulated by tumor suppressive miR-375 induced by histone acetylation in esophageal squamous cell carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T03%3A51%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20novel%20molecular%20targets%20regulated%20by%20tumor%20suppressive%20miR-375%20induced%20by%20histone%20acetylation%20in%20esophageal%20squamous%20cell%20carcinoma&rft.jtitle=International%20journal%20of%20oncology&rft.au=ISOZAKI,%20YUKA&rft.date=2012-09-01&rft.volume=41&rft.issue=3&rft.spage=985&rft.epage=994&rft.pages=985-994&rft.issn=1019-6439&rft.eissn=1791-2423&rft_id=info:doi/10.3892/ijo.2012.1537&rft_dat=%3Cproquest_cross%3E1932339417%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1932339417&rft_id=info:pmid/22752059&rfr_iscdi=true |