miR-221-induced PUMA silencing mediates immune evasion of bladder cancer cells
Immune evasion of cancer cells is mainly due to the impaired transduction of apoptotic signals from immune cells to cancer cells, as well as inhibition of subsequent apoptosis signal cascades within the cancer cells. Over the past few decades, the research has focused more on the impaired transducti...
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| Veröffentlicht in: | International journal of oncology 2015-03, Vol.46 (3), p.1169-1180 |
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| creator | FU, BIN WANG, YIBING ZHANG, XIALI LANG, BIN ZHOU, XIAOCHENG XU, XIAOYUAN ZENG, TAO LIU, WEIPENG ZHANG, XU GUO, JU WANG, GONGXIAN |
| description | Immune evasion of cancer cells is mainly due to the impaired transduction of apoptotic signals from immune cells to cancer cells, as well as inhibition of subsequent apoptosis signal cascades within the cancer cells. Over the past few decades, the research has focused more on the impaired transduction of the apoptotic signal from immune cells to cancer cells, rather than inhibition of the intracellular signaling pathways. In this study, miR-221 inhibitor was transfected into bladder cancer cell lines 5637, J82 and T24 to repress the expression of miR-221. As a result, the repression of miR-221 on p53 upregulated modulator of apoptosis (PUMA) was abolished, resulting in increased expression of the pro-apoptotic Bax and reduced expression of the anti-apoptotic Bcl-2, which promotes apoptosis of bladder cancer cells. The expression of MMP-2, MMP-9 and VEGF-C were reduced, resulting in reduced invasiveness and infiltration capability of bladder cancer cells, thereby inhibiting the immune evasion of bladder cancer cells. |
| doi_str_mv | 10.3892/ijo.2015.2837 |
| format | Article |
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Over the past few decades, the research has focused more on the impaired transduction of the apoptotic signal from immune cells to cancer cells, rather than inhibition of the intracellular signaling pathways. In this study, miR-221 inhibitor was transfected into bladder cancer cell lines 5637, J82 and T24 to repress the expression of miR-221. As a result, the repression of miR-221 on p53 upregulated modulator of apoptosis (PUMA) was abolished, resulting in increased expression of the pro-apoptotic Bax and reduced expression of the anti-apoptotic Bcl-2, which promotes apoptosis of bladder cancer cells. The expression of MMP-2, MMP-9 and VEGF-C were reduced, resulting in reduced invasiveness and infiltration capability of bladder cancer cells, thereby inhibiting the immune evasion of bladder cancer cells.</description><identifier>ISSN: 1019-6439</identifier><identifier>EISSN: 1791-2423</identifier><identifier>DOI: 10.3892/ijo.2015.2837</identifier><identifier>PMID: 25585941</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Angiogenesis ; Apoptosis ; Apoptosis Regulatory Proteins - genetics ; bcl-2-Associated X Protein - genetics ; Binding sites ; Bladder cancer ; Cell Proliferation - drug effects ; Cellular proteins ; Cellular signal transduction ; Development and progression ; Extracellular matrix ; Gene expression ; Gene Expression Regulation, Neoplastic - drug effects ; Gene Expression Regulation, Neoplastic - physiology ; Genetic aspects ; Health aspects ; Humans ; immune escape ; Immune response ; Metastasis ; MicroRNAs ; MicroRNAs - antagonists & inhibitors ; MicroRNAs - physiology ; miR-221 ; Neoplasm Invasiveness ; Oligoribonucleotides, Antisense - genetics ; Oligoribonucleotides, Antisense - pharmacology ; Proteins ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins c-bcl-2 - genetics ; Regulation ; RNA Interference - drug effects ; RNA Interference - physiology ; Signal transduction ; Studies ; Tumor Cells, Cultured ; Tumor Escape - genetics ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - immunology ; Urinary Bladder Neoplasms - pathology ; Vascular endothelial growth factor</subject><ispartof>International journal of oncology, 2015-03, Vol.46 (3), p.1169-1180</ispartof><rights>Copyright © 2015, Spandidos Publications</rights><rights>COPYRIGHT 2015 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-1d005ff0f9c5db2136e83b69c05839f15644954385ae94ba7b730073a9a9d6d23</citedby><cites>FETCH-LOGICAL-c517t-1d005ff0f9c5db2136e83b69c05839f15644954385ae94ba7b730073a9a9d6d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,5556,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25585941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FU, BIN</creatorcontrib><creatorcontrib>WANG, YIBING</creatorcontrib><creatorcontrib>ZHANG, XIALI</creatorcontrib><creatorcontrib>LANG, BIN</creatorcontrib><creatorcontrib>ZHOU, XIAOCHENG</creatorcontrib><creatorcontrib>XU, XIAOYUAN</creatorcontrib><creatorcontrib>ZENG, TAO</creatorcontrib><creatorcontrib>LIU, WEIPENG</creatorcontrib><creatorcontrib>ZHANG, XU</creatorcontrib><creatorcontrib>GUO, JU</creatorcontrib><creatorcontrib>WANG, GONGXIAN</creatorcontrib><title>miR-221-induced PUMA silencing mediates immune evasion of bladder cancer cells</title><title>International journal of oncology</title><addtitle>Int J Oncol</addtitle><description>Immune evasion of cancer cells is mainly due to the impaired transduction of apoptotic signals from immune cells to cancer cells, as well as inhibition of subsequent apoptosis signal cascades within the cancer cells. Over the past few decades, the research has focused more on the impaired transduction of the apoptotic signal from immune cells to cancer cells, rather than inhibition of the intracellular signaling pathways. In this study, miR-221 inhibitor was transfected into bladder cancer cell lines 5637, J82 and T24 to repress the expression of miR-221. As a result, the repression of miR-221 on p53 upregulated modulator of apoptosis (PUMA) was abolished, resulting in increased expression of the pro-apoptotic Bax and reduced expression of the anti-apoptotic Bcl-2, which promotes apoptosis of bladder cancer cells. The expression of MMP-2, MMP-9 and VEGF-C were reduced, resulting in reduced invasiveness and infiltration capability of bladder cancer cells, thereby inhibiting the immune evasion of bladder cancer cells.</description><subject>Angiogenesis</subject><subject>Apoptosis</subject><subject>Apoptosis Regulatory Proteins - genetics</subject><subject>bcl-2-Associated X Protein - genetics</subject><subject>Binding sites</subject><subject>Bladder cancer</subject><subject>Cell Proliferation - drug effects</subject><subject>Cellular proteins</subject><subject>Cellular signal transduction</subject><subject>Development and progression</subject><subject>Extracellular matrix</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Gene Expression Regulation, Neoplastic - physiology</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>immune escape</subject><subject>Immune response</subject><subject>Metastasis</subject><subject>MicroRNAs</subject><subject>MicroRNAs - antagonists & inhibitors</subject><subject>MicroRNAs - physiology</subject><subject>miR-221</subject><subject>Neoplasm Invasiveness</subject><subject>Oligoribonucleotides, Antisense - genetics</subject><subject>Oligoribonucleotides, Antisense - pharmacology</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins c-bcl-2 - genetics</subject><subject>Regulation</subject><subject>RNA Interference - drug effects</subject><subject>RNA Interference - physiology</subject><subject>Signal transduction</subject><subject>Studies</subject><subject>Tumor Cells, Cultured</subject><subject>Tumor Escape - genetics</subject><subject>Urinary Bladder Neoplasms - genetics</subject><subject>Urinary Bladder Neoplasms - immunology</subject><subject>Urinary Bladder Neoplasms - pathology</subject><subject>Vascular endothelial growth factor</subject><issn>1019-6439</issn><issn>1791-2423</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpV0cFvFCEUBnBibGytHr2aSUz0xPrgwcxw3DS2NanVGHsmDDAtmxlYhxmT_vcy2bZpTx-HXx7wPkI-MNhgq_jXsEsbDkxueIvNK3LCGsUoFxxflzMwRWuB6pi8zXkHwKUE9oYcl2ylEuyEXI_hN-Wc0RDdYr2rft382FY5DD7aEG-r0btgZp-rMI5L9JX_Z3JIsUp91Q3GOT9V1kS7hh-G_I4c9WbI_v1DnpKb829_zi7p1c-L72fbK2ola2bKHIDse-iVla7jDGvfYlcrC7JF1TNZC6GkwFYar0Rnmq5BgAaNMsrVjuMp-XSYu5_S38XnWe_SMsVypWYKOXKF7TN1awavQ-zTPBk7hmz1VmADIBSs6vMzdefNMN_lNCxz-Wd-CekB2inlPPle76cwmuleM9BrGbqUodcy9FpG8R8fHrl0ZY9P-nH7BXw5gLw30QWX8pMpk6ioKSBlrFb4Hy6ejaA</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>FU, BIN</creator><creator>WANG, YIBING</creator><creator>ZHANG, XIALI</creator><creator>LANG, BIN</creator><creator>ZHOU, XIAOCHENG</creator><creator>XU, XIAOYUAN</creator><creator>ZENG, TAO</creator><creator>LIU, WEIPENG</creator><creator>ZHANG, XU</creator><creator>GUO, JU</creator><creator>WANG, GONGXIAN</creator><general>D.A. 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| ispartof | International journal of oncology, 2015-03, Vol.46 (3), p.1169-1180 |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Angiogenesis Apoptosis Apoptosis Regulatory Proteins - genetics bcl-2-Associated X Protein - genetics Binding sites Bladder cancer Cell Proliferation - drug effects Cellular proteins Cellular signal transduction Development and progression Extracellular matrix Gene expression Gene Expression Regulation, Neoplastic - drug effects Gene Expression Regulation, Neoplastic - physiology Genetic aspects Health aspects Humans immune escape Immune response Metastasis MicroRNAs MicroRNAs - antagonists & inhibitors MicroRNAs - physiology miR-221 Neoplasm Invasiveness Oligoribonucleotides, Antisense - genetics Oligoribonucleotides, Antisense - pharmacology Proteins Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins c-bcl-2 - genetics Regulation RNA Interference - drug effects RNA Interference - physiology Signal transduction Studies Tumor Cells, Cultured Tumor Escape - genetics Urinary Bladder Neoplasms - genetics Urinary Bladder Neoplasms - immunology Urinary Bladder Neoplasms - pathology Vascular endothelial growth factor |
| title | miR-221-induced PUMA silencing mediates immune evasion of bladder cancer cells |
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