Measurement of Chlorophyll Loss Due to Phytoremediation of Ag Nanoparticles in the First-Year Laboratory

A two-week experiment is presented in which students can observe the impact of nanoparticles on the concentration of chlorophyll in plants. First-year students in an introductory nanotechnology laboratory course and a general chemistry laboratory course synthesized silver nanoparticles and then exposed stalks of Egeria densa (E. densa), a common waterweed, to the nanoparticle solution for 1 week. In the following session, students extracted chlorophyll from the plants and measured its concentration using a visible spectrometer. Compared to other, similar lab activities, this experiment generates a lower amount of waste, requires a shorter duration of plant growth, and involves the measurement of chemical species in order to determine the toxicological effects of nanomaterials. Additional ideas are discussed for implementing the experiment in high school, general chemistry, and other courses.