Ultrasensitive aptasensing of lysozyme by exploiting the synergistic effect of gold nanoparticle-modified reduced graphene oxide and MWCNTs in a chitosan matrix
The authors describe a lysozyme aptasensor based on the use of gold nanoparticles (AuNP) assembled on the carbon nanotubes, graphene oxide and chitosan. An electrochemical impedance spectroscopic study was performed to demonstrate the synergistic effect of the MWCNT-AuNP, Chit-AuNP and GO-AuNP compo...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2017-09, Vol.184 (9), p.3405-3413 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Heydari-Bafrooei, Esmaeil Askari, Samira |
| description | The authors describe a lysozyme aptasensor based on the use of gold nanoparticles (AuNP) assembled on the carbon nanotubes, graphene oxide and chitosan. An electrochemical impedance spectroscopic study was performed to demonstrate the synergistic effect of the MWCNT-AuNP, Chit-AuNP and GO-AuNP composites and the order of the layers affect the performance of aptasensing. Different parameters were optimized in order to obtain successful and sensitive detection of lysozyme in urine and saliva samples. A plot of charge-transfer resistance versus the logarithm of the lysozyme concentration is linear in the 0.02 to 250 pM concentration range, with a 9 fM detection limit (at an S/N ratio of 3). The aptasensor is highly specific and selective. Clinical analyses were performed and data were found to be in good agreement with those obtained by HPLC.
Graphical abstract
Schematic of the procedure for aptamer-based detection of lysozyme using the electrochemical signal of the K
3
[Fe(CN)
6
]/K
4
[Fe(CN)
6
] amplified by AuNP, rGO and MWCNT. This assay has high sensitivity and good selectivity. It can presumably be transferred to other protein detection schemes. |
| doi_str_mv | 10.1007/s00604-017-2356-3 |
| format | Article |
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Graphical abstract
Schematic of the procedure for aptamer-based detection of lysozyme using the electrochemical signal of the K
3
[Fe(CN)
6
]/K
4
[Fe(CN)
6
] amplified by AuNP, rGO and MWCNT. This assay has high sensitivity and good selectivity. It can presumably be transferred to other protein detection schemes.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-017-2356-3</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analysis ; Analytical Chemistry ; Characterization and Evaluation of Materials ; Charge transfer ; Chemistry ; Chemistry and Materials Science ; Chitosan ; Electric properties ; Electrochemical impedance spectroscopy ; Gold ; Graphene ; Graphite ; Lysozyme ; Microengineering ; Multi wall carbon nanotubes ; Nanochemistry ; Nanoparticles ; Nanotechnology ; Nanotubes ; Order parameters ; Original Paper ; Parameter sensitivity ; Saliva ; Signal to noise ratio ; Spectroscopic analysis ; Synergistic effect ; Urine</subject><ispartof>Mikrochimica acta (1966), 2017-09, Vol.184 (9), p.3405-3413</ispartof><rights>Springer-Verlag GmbH Austria 2017</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Microchimica Acta is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-1477fbd7992b310e92577da113abfdd010105e0dfcb2a13b12985a4be02931d3</citedby><cites>FETCH-LOGICAL-c355t-1477fbd7992b310e92577da113abfdd010105e0dfcb2a13b12985a4be02931d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-017-2356-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-017-2356-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Heydari-Bafrooei, Esmaeil</creatorcontrib><creatorcontrib>Askari, Samira</creatorcontrib><title>Ultrasensitive aptasensing of lysozyme by exploiting the synergistic effect of gold nanoparticle-modified reduced graphene oxide and MWCNTs in a chitosan matrix</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><description>The authors describe a lysozyme aptasensor based on the use of gold nanoparticles (AuNP) assembled on the carbon nanotubes, graphene oxide and chitosan. An electrochemical impedance spectroscopic study was performed to demonstrate the synergistic effect of the MWCNT-AuNP, Chit-AuNP and GO-AuNP composites and the order of the layers affect the performance of aptasensing. Different parameters were optimized in order to obtain successful and sensitive detection of lysozyme in urine and saliva samples. A plot of charge-transfer resistance versus the logarithm of the lysozyme concentration is linear in the 0.02 to 250 pM concentration range, with a 9 fM detection limit (at an S/N ratio of 3). The aptasensor is highly specific and selective. Clinical analyses were performed and data were found to be in good agreement with those obtained by HPLC.
Graphical abstract
Schematic of the procedure for aptamer-based detection of lysozyme using the electrochemical signal of the K
3
[Fe(CN)
6
]/K
4
[Fe(CN)
6
] amplified by AuNP, rGO and MWCNT. This assay has high sensitivity and good selectivity. It can presumably be transferred to other protein detection schemes.</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Charge transfer</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chitosan</subject><subject>Electric properties</subject><subject>Electrochemical impedance spectroscopy</subject><subject>Gold</subject><subject>Graphene</subject><subject>Graphite</subject><subject>Lysozyme</subject><subject>Microengineering</subject><subject>Multi wall carbon nanotubes</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Nanotubes</subject><subject>Order parameters</subject><subject>Original Paper</subject><subject>Parameter sensitivity</subject><subject>Saliva</subject><subject>Signal to noise ratio</subject><subject>Spectroscopic analysis</subject><subject>Synergistic effect</subject><subject>Urine</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kU1v1DAQhi1EJZa2P4CbJc4p_ojj5lit-JIKXBZxtBx7nHWV2MH2og2_pj8Vr8KBC5rDaGbex2PNi9AbSu4oIfJdJqQjbUOobBgXXcNfoB1tedcIIvlLtCOE1WYn2Sv0OucnUoUda3fo-ftUks4Qsi_-F2C9lK0KI44OT2uOv9cZ8LBiOC9TrKo6KUfAeQ2QRp-LNxicA1MuwBgni4MOcdGpTiZo5mi982BxAnsyNY9JL0cIgOPZ27oxWPzlx_7rIWMfsMbm6EvMOuBZl-TPN-jK6SnD7d98jQ4f3h_2n5rHbx8_7x8eG8OFKA1tpXSDlX3PBk4J9ExIaTWlXA_OWkJrCCDWmYFpygfK-nuh2wEI6zm1_Bq93Z5dUvx5glzUUzylUDcq2jMp-T3loqruNtWoJ1A-uFiPZ2pYmL2JAZyv_QdJRXWDsa4CdANMijkncGpJftZpVZSoi3FqM05VP9TFOMUrwzYmV20YIf3zlf9CfwCic54p</recordid><startdate>20170901</startdate><enddate>20170901</enddate><creator>Heydari-Bafrooei, Esmaeil</creator><creator>Askari, Samira</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>M0S</scope><scope>M1P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20170901</creationdate><title>Ultrasensitive aptasensing of lysozyme by exploiting the synergistic effect of gold nanoparticle-modified reduced graphene oxide and MWCNTs in a chitosan matrix</title><author>Heydari-Bafrooei, Esmaeil ; Askari, Samira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-1477fbd7992b310e92577da113abfdd010105e0dfcb2a13b12985a4be02931d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Charge transfer</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chitosan</topic><topic>Electric properties</topic><topic>Electrochemical impedance spectroscopy</topic><topic>Gold</topic><topic>Graphene</topic><topic>Graphite</topic><topic>Lysozyme</topic><topic>Microengineering</topic><topic>Multi wall carbon nanotubes</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Nanotubes</topic><topic>Order parameters</topic><topic>Original Paper</topic><topic>Parameter sensitivity</topic><topic>Saliva</topic><topic>Signal to noise ratio</topic><topic>Spectroscopic analysis</topic><topic>Synergistic effect</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heydari-Bafrooei, Esmaeil</creatorcontrib><creatorcontrib>Askari, Samira</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heydari-Bafrooei, Esmaeil</au><au>Askari, Samira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrasensitive aptasensing of lysozyme by exploiting the synergistic effect of gold nanoparticle-modified reduced graphene oxide and MWCNTs in a chitosan matrix</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><date>2017-09-01</date><risdate>2017</risdate><volume>184</volume><issue>9</issue><spage>3405</spage><epage>3413</epage><pages>3405-3413</pages><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>The authors describe a lysozyme aptasensor based on the use of gold nanoparticles (AuNP) assembled on the carbon nanotubes, graphene oxide and chitosan. An electrochemical impedance spectroscopic study was performed to demonstrate the synergistic effect of the MWCNT-AuNP, Chit-AuNP and GO-AuNP composites and the order of the layers affect the performance of aptasensing. Different parameters were optimized in order to obtain successful and sensitive detection of lysozyme in urine and saliva samples. A plot of charge-transfer resistance versus the logarithm of the lysozyme concentration is linear in the 0.02 to 250 pM concentration range, with a 9 fM detection limit (at an S/N ratio of 3). The aptasensor is highly specific and selective. Clinical analyses were performed and data were found to be in good agreement with those obtained by HPLC.
Graphical abstract
Schematic of the procedure for aptamer-based detection of lysozyme using the electrochemical signal of the K
3
[Fe(CN)
6
]/K
4
[Fe(CN)
6
] amplified by AuNP, rGO and MWCNT. This assay has high sensitivity and good selectivity. It can presumably be transferred to other protein detection schemes.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><doi>10.1007/s00604-017-2356-3</doi><tpages>9</tpages></addata></record> |
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| subjects | Analysis Analytical Chemistry Characterization and Evaluation of Materials Charge transfer Chemistry Chemistry and Materials Science Chitosan Electric properties Electrochemical impedance spectroscopy Gold Graphene Graphite Lysozyme Microengineering Multi wall carbon nanotubes Nanochemistry Nanoparticles Nanotechnology Nanotubes Order parameters Original Paper Parameter sensitivity Saliva Signal to noise ratio Spectroscopic analysis Synergistic effect Urine |
| title | Ultrasensitive aptasensing of lysozyme by exploiting the synergistic effect of gold nanoparticle-modified reduced graphene oxide and MWCNTs in a chitosan matrix |
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