Gelatin-loaded p(HEMA-GMA) cryogel for high-capacity immobilization of horseradish peroxidase
Poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [p(HEMA-GMA)] cryogel discs were prepared under sub-zero temperatures. Gelatin was attached covalently on the p(HEMA-GMA) cryogel discs and reversible immobilization of horseradish peroxidase (HRP) was performed. The p(HEMA-GMA) cryogel discs w...
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| Veröffentlicht in: | Artificial cells, nanomedicine, and biotechnology nanomedicine, and biotechnology, 2016-11, Vol.44 (7), p.1708-1713 |
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| container_title | Artificial cells, nanomedicine, and biotechnology |
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| creator | Soomro, Rabel Perçin, Işık Memon, Najma Iqbal Bhanger, Muhammad Denizli, Adil |
| description | Poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [p(HEMA-GMA)] cryogel discs were prepared under sub-zero temperatures. Gelatin was attached covalently on the p(HEMA-GMA) cryogel discs and reversible immobilization of horseradish peroxidase (HRP) was performed. The p(HEMA-GMA) cryogel discs were characterized by swelling tests, scanning electron microscopy, and surface area measurements. HRP immobilization capacity of p(HEMA-GMA)/gelatin cryogel discs was 24.8 mg/g. Removal of phenol from aqueous solutions was performed using HRP immobilized p(HEMA-GMA)/gelatin cryogel. It was observed that within 2 h of contact time, the percentage of phenol removal reaches up to 91% in the presence of H
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| doi_str_mv | 10.3109/21691401.2015.1089252 |
| format | Article |
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2
O
2.</description><identifier>ISSN: 2169-1401</identifier><identifier>EISSN: 2169-141X</identifier><identifier>DOI: 10.3109/21691401.2015.1089252</identifier><identifier>PMID: 26508304</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Aqueous solutions ; Covalence ; cryogel ; Cryogels - chemistry ; Disks ; Electron microscopy ; Enzymes, Immobilized - chemistry ; Gelatin ; Gelatin - chemistry ; glycidyl methacrylate ; Horseradish peroxidase ; Horseradish Peroxidase - chemistry ; Hydrogen peroxide ; Immobilization ; Peroxidase ; phenol ; Phenols ; Polyhydroxyethyl methacrylate ; Polyhydroxyethyl Methacrylate - analogs & derivatives ; Polyhydroxyethyl Methacrylate - chemistry ; Scanning electron microscopy</subject><ispartof>Artificial cells, nanomedicine, and biotechnology, 2016-11, Vol.44 (7), p.1708-1713</ispartof><rights>2015 Taylor & Francis 2015</rights><rights>2015 Taylor & Francis</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-3c71fd94d4a3d1c9a3075bd170a569c4b7dcde3a96ba0df6703fe56f3e002e033</citedby><cites>FETCH-LOGICAL-c474t-3c71fd94d4a3d1c9a3075bd170a569c4b7dcde3a96ba0df6703fe56f3e002e033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26508304$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soomro, Rabel</creatorcontrib><creatorcontrib>Perçin, Işık</creatorcontrib><creatorcontrib>Memon, Najma</creatorcontrib><creatorcontrib>Iqbal Bhanger, Muhammad</creatorcontrib><creatorcontrib>Denizli, Adil</creatorcontrib><title>Gelatin-loaded p(HEMA-GMA) cryogel for high-capacity immobilization of horseradish peroxidase</title><title>Artificial cells, nanomedicine, and biotechnology</title><addtitle>Artif Cells Nanomed Biotechnol</addtitle><description>Poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [p(HEMA-GMA)] cryogel discs were prepared under sub-zero temperatures. Gelatin was attached covalently on the p(HEMA-GMA) cryogel discs and reversible immobilization of horseradish peroxidase (HRP) was performed. The p(HEMA-GMA) cryogel discs were characterized by swelling tests, scanning electron microscopy, and surface area measurements. HRP immobilization capacity of p(HEMA-GMA)/gelatin cryogel discs was 24.8 mg/g. Removal of phenol from aqueous solutions was performed using HRP immobilized p(HEMA-GMA)/gelatin cryogel. It was observed that within 2 h of contact time, the percentage of phenol removal reaches up to 91% in the presence of H
2
O
2.</description><subject>Aqueous solutions</subject><subject>Covalence</subject><subject>cryogel</subject><subject>Cryogels - chemistry</subject><subject>Disks</subject><subject>Electron microscopy</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Gelatin</subject><subject>Gelatin - chemistry</subject><subject>glycidyl methacrylate</subject><subject>Horseradish peroxidase</subject><subject>Horseradish Peroxidase - chemistry</subject><subject>Hydrogen peroxide</subject><subject>Immobilization</subject><subject>Peroxidase</subject><subject>phenol</subject><subject>Phenols</subject><subject>Polyhydroxyethyl methacrylate</subject><subject>Polyhydroxyethyl Methacrylate - analogs & derivatives</subject><subject>Polyhydroxyethyl Methacrylate - chemistry</subject><subject>Scanning electron microscopy</subject><issn>2169-1401</issn><issn>2169-141X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAURi0EolXpI4AisSmLDPfGdpzsGFVlitSKDUhskOX4p-PKiYOdURmenoSZdsEC4c21rs73WdYh5DXCiiK07yusW2SAqwqQrxCatuLVM3K67Etk-O350x3whJznfA_zabAWnL0kJ1XNoaHATsn3jQ1q8kMZojLWFOPF9dXtutzcrt8VOu3jnQ2Fi6nY-rttqdWotJ_2he_72Pngf83ROBTRFduYsk3K-LwtRpviT29Utq_IC6dCtufHeUa-frz6cnld3nzefLpc35SaCTaVVAt0pmWGKWpQt4qC4J1BAYrXrWadMNpYqtq6U2BcLYA6y2tHLUBlgdIzcnHoHVP8sbN5kr3P2oagBht3WWLTYtMAAvsPFBtKhfiDvv0LvY-7NMwfkTj38aZCvlD8QOkUc07WyTH5XqW9RJCLLfloSy625NHWnHtzbN91vTVPqUc3M_DhAPhhNtCrh5iCkZPah5hcUoP2een_1xu_Aeinogo</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Soomro, Rabel</creator><creator>Perçin, Işık</creator><creator>Memon, Najma</creator><creator>Iqbal Bhanger, Muhammad</creator><creator>Denizli, Adil</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20161101</creationdate><title>Gelatin-loaded p(HEMA-GMA) cryogel for high-capacity immobilization of horseradish peroxidase</title><author>Soomro, Rabel ; Perçin, Işık ; Memon, Najma ; Iqbal Bhanger, Muhammad ; Denizli, Adil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-3c71fd94d4a3d1c9a3075bd170a569c4b7dcde3a96ba0df6703fe56f3e002e033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aqueous solutions</topic><topic>Covalence</topic><topic>cryogel</topic><topic>Cryogels - chemistry</topic><topic>Disks</topic><topic>Electron microscopy</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Gelatin</topic><topic>Gelatin - chemistry</topic><topic>glycidyl methacrylate</topic><topic>Horseradish peroxidase</topic><topic>Horseradish Peroxidase - chemistry</topic><topic>Hydrogen peroxide</topic><topic>Immobilization</topic><topic>Peroxidase</topic><topic>phenol</topic><topic>Phenols</topic><topic>Polyhydroxyethyl methacrylate</topic><topic>Polyhydroxyethyl Methacrylate - analogs & derivatives</topic><topic>Polyhydroxyethyl Methacrylate - chemistry</topic><topic>Scanning electron microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soomro, Rabel</creatorcontrib><creatorcontrib>Perçin, Işık</creatorcontrib><creatorcontrib>Memon, Najma</creatorcontrib><creatorcontrib>Iqbal Bhanger, Muhammad</creatorcontrib><creatorcontrib>Denizli, Adil</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soomro, Rabel</au><au>Perçin, Işık</au><au>Memon, Najma</au><au>Iqbal Bhanger, Muhammad</au><au>Denizli, Adil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gelatin-loaded p(HEMA-GMA) cryogel for high-capacity immobilization of horseradish peroxidase</atitle><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle><addtitle>Artif Cells Nanomed Biotechnol</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>44</volume><issue>7</issue><spage>1708</spage><epage>1713</epage><pages>1708-1713</pages><issn>2169-1401</issn><eissn>2169-141X</eissn><abstract>Poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [p(HEMA-GMA)] cryogel discs were prepared under sub-zero temperatures. Gelatin was attached covalently on the p(HEMA-GMA) cryogel discs and reversible immobilization of horseradish peroxidase (HRP) was performed. The p(HEMA-GMA) cryogel discs were characterized by swelling tests, scanning electron microscopy, and surface area measurements. HRP immobilization capacity of p(HEMA-GMA)/gelatin cryogel discs was 24.8 mg/g. Removal of phenol from aqueous solutions was performed using HRP immobilized p(HEMA-GMA)/gelatin cryogel. It was observed that within 2 h of contact time, the percentage of phenol removal reaches up to 91% in the presence of H
2
O
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Aqueous solutions Covalence cryogel Cryogels - chemistry Disks Electron microscopy Enzymes, Immobilized - chemistry Gelatin Gelatin - chemistry glycidyl methacrylate Horseradish peroxidase Horseradish Peroxidase - chemistry Hydrogen peroxide Immobilization Peroxidase phenol Phenols Polyhydroxyethyl methacrylate Polyhydroxyethyl Methacrylate - analogs & derivatives Polyhydroxyethyl Methacrylate - chemistry Scanning electron microscopy |
| title | Gelatin-loaded p(HEMA-GMA) cryogel for high-capacity immobilization of horseradish peroxidase |
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