Antimicrobial Activity of Chlorhexidine against Multi-Species Biofilm Formation
In the present work, the efficacy of the Ti–7.5Mo alloy nanotube and Ti–7.5Mo alloy nanotube with chlorhexidine against bacterial biofilm formation was evaluated. Nanotubes were processed using anodization in 0.25% NH4F electrolyte solution. Biofilms were cultured in discs immersed in sterile brain...
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| Veröffentlicht in: | Materials science forum 2017-07, Vol.899, p.237-242 |
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| creator | do Amaral Escada, Ana Lucia Pereira, Cristiane Aparecida Rosifini Alves Claro, Ana Paula Jorge, Antonio Olavo Cardoso |
| description | In the present work, the efficacy of the Ti–7.5Mo alloy nanotube and Ti–7.5Mo alloy nanotube with chlorhexidine against bacterial biofilm formation was evaluated. Nanotubes were processed using anodization in 0.25% NH4F electrolyte solution. Biofilms were cultured in discs immersed in sterile brain heart infusion broth (BHI) containing 5% sucrose, inoculated with microbial suspension (106 cells/ml) and incubated for 5 days. Next, the discs were placed in tubes with sterile physiological solution 0.9% sodium chloride (NaCl) and sonicated to disperse the biofilms. Tenfold serial dilutions were carried and aliquots seeded in selective agar, which were then incubated for 48 h. Then, the numbers CFU/ml (log 10) were counted and analyzed statistically. Scanning electron microscopy (SEM) on discs with biofilms groups and contact angle was carried out. The results show that there is no difference in bacterial adhesion between of the Ti–7.5Mo alloy nanotube and Ti–7.5Mo alloy nanotube with chlorhexidine. |
| doi_str_mv | 10.4028/www.scientific.net/MSF.899.237 |
| format | Article |
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Nanotubes were processed using anodization in 0.25% NH4F electrolyte solution. Biofilms were cultured in discs immersed in sterile brain heart infusion broth (BHI) containing 5% sucrose, inoculated with microbial suspension (106 cells/ml) and incubated for 5 days. Next, the discs were placed in tubes with sterile physiological solution 0.9% sodium chloride (NaCl) and sonicated to disperse the biofilms. Tenfold serial dilutions were carried and aliquots seeded in selective agar, which were then incubated for 48 h. Then, the numbers CFU/ml (log 10) were counted and analyzed statistically. Scanning electron microscopy (SEM) on discs with biofilms groups and contact angle was carried out. The results show that there is no difference in bacterial adhesion between of the Ti–7.5Mo alloy nanotube and Ti–7.5Mo alloy nanotube with chlorhexidine.</description><identifier>ISSN: 0255-5476</identifier><identifier>ISSN: 1662-9752</identifier><identifier>EISSN: 1662-9752</identifier><identifier>DOI: 10.4028/www.scientific.net/MSF.899.237</identifier><language>eng</language><publisher>Pfaffikon: Trans Tech Publications Ltd</publisher><subject>Adhesion ; Agar ; Anodizing ; Antiinfectives and antibacterials ; Bacteria ; Biofilms ; Brain ; Chlorhexidine ; Contact angle ; Dilution ; Disks ; Electrolytic cells ; Microorganisms ; Nanotubes ; Scanning electron microscopy ; Sodium chloride ; Sucrose ; Titanium base alloys ; Tubes</subject><ispartof>Materials science forum, 2017-07, Vol.899, p.237-242</ispartof><rights>2017 Trans Tech Publications Ltd</rights><rights>Copyright Trans Tech Publications Ltd. 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The results show that there is no difference in bacterial adhesion between of the Ti–7.5Mo alloy nanotube and Ti–7.5Mo alloy nanotube with chlorhexidine.</description><subject>Adhesion</subject><subject>Agar</subject><subject>Anodizing</subject><subject>Antiinfectives and antibacterials</subject><subject>Bacteria</subject><subject>Biofilms</subject><subject>Brain</subject><subject>Chlorhexidine</subject><subject>Contact angle</subject><subject>Dilution</subject><subject>Disks</subject><subject>Electrolytic cells</subject><subject>Microorganisms</subject><subject>Nanotubes</subject><subject>Scanning electron microscopy</subject><subject>Sodium chloride</subject><subject>Sucrose</subject><subject>Titanium base alloys</subject><subject>Tubes</subject><issn>0255-5476</issn><issn>1662-9752</issn><issn>1662-9752</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqNkE1LAzEURYMoWKv_YUBwN9Nk8jGTjViLVaGli-o6pJnEpsxMapJa---NVNClq7e571zuAeAGwYLAsh7t9_siKKv7aI1VRa_jaL6cFjXnRYmrEzBAjJU5r2h5CgawpDSnpGLn4CKEDYQY1YgNwGKc3jurvFtZ2WZjFe2HjYfMmWyybp1f60_b2F5n8k3aPsRsvmujzZdbnZpDdm-dsW2XTZ3vZLSuvwRnRrZBX_3cIXidPrxMnvLZ4vF5Mp7lqkSwypniGBkGG6zgihEsG4IUZRUzRDewUVTWpNGYcIJqU3FO1IpWtUKESYkgwXgIro_crXfvOx2i2Lid71OlQByxmkLGWUrdHlNpXwheG7H1tpP-IBAU3xJFkih-JYokUSSJIkkUSWIC3B0B0cu0Xqv1n57_Ib4AR9eD3A</recordid><startdate>20170703</startdate><enddate>20170703</enddate><creator>do Amaral Escada, Ana Lucia</creator><creator>Pereira, Cristiane Aparecida</creator><creator>Rosifini Alves Claro, Ana Paula</creator><creator>Jorge, Antonio Olavo Cardoso</creator><general>Trans Tech Publications Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SR</scope><scope>7XB</scope><scope>88I</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>M2P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20170703</creationdate><title>Antimicrobial Activity of Chlorhexidine against Multi-Species Biofilm Formation</title><author>do Amaral Escada, Ana Lucia ; 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Nanotubes were processed using anodization in 0.25% NH4F electrolyte solution. Biofilms were cultured in discs immersed in sterile brain heart infusion broth (BHI) containing 5% sucrose, inoculated with microbial suspension (106 cells/ml) and incubated for 5 days. Next, the discs were placed in tubes with sterile physiological solution 0.9% sodium chloride (NaCl) and sonicated to disperse the biofilms. Tenfold serial dilutions were carried and aliquots seeded in selective agar, which were then incubated for 48 h. Then, the numbers CFU/ml (log 10) were counted and analyzed statistically. Scanning electron microscopy (SEM) on discs with biofilms groups and contact angle was carried out. 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| subjects | Adhesion Agar Anodizing Antiinfectives and antibacterials Bacteria Biofilms Brain Chlorhexidine Contact angle Dilution Disks Electrolytic cells Microorganisms Nanotubes Scanning electron microscopy Sodium chloride Sucrose Titanium base alloys Tubes |
| title | Antimicrobial Activity of Chlorhexidine against Multi-Species Biofilm Formation |
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