MOLECULAR IDENTIFICATION AND ANTIBIOGRAM OF Enterococcus spp. ISOLATED ON ENTEROCOCCUS SELECTIVE DIFFERENTIAL (ESD) MEDIA FROM MEAT, MEAT PRODUCTS AND SEAFOOD IN LIBYA
This study was conducted to investigate the presence of Enterococcus spp. in meat, meat products and seafood. A hundred and four samples were randomly collected from different geographic localities in Libya. The samples were subjected to microbiological analysis for enumeration and isolation of Ente...
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Veröffentlicht in: | Journal of microbiology, biotechnology and food sciences biotechnology and food sciences, 2017-06, Vol.6 (6), p.1264-1268 |
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creator | T. Naas, Hesham Almajdoubi, Zaid M. Garbaj, Aboubaker M. Azwai, Salah T. Gammoudi, Fatim K. Abolghait, Said A. Moawad, Ashraf Barbieri, Ilaria L. Eshamah, Hanan M. Eldaghayes, Ibrahim |
description | This study was conducted to investigate the presence of Enterococcus spp. in meat, meat products and seafood. A hundred and four samples were randomly collected from different geographic localities in Libya. The samples were subjected to microbiological analysis for enumeration and isolation of Enterococcus spp. by conventional cultural and molecular identification using PCR and partial sequencing of 16S rDNA techniques. Out of 104 samples, 73 (70.2%) isolates were found to be enterococci based on their cultural characteristics on ESD medium. However, out of 36 samples subjected to molecular identification, only six isolates were confirmed to be Enterococcus spp. using PCR and partial sequencing of 16S rDNA technique. All enterococci strains tested for their antibiotic sensitivity profiles showed high percentage of multi-resistance phenotype. These results can be used for further studies on enterococci as an emerging food borne pathogen and its role in human infection in Libya and would suggest that meat, meat products and seafood might play a role in the spreading of enterococci through the food chain with antimicrobial resistance characteristics. |
doi_str_mv | 10.15414/jmbfs.2017.6.6.1264-1268 |
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Naas, Hesham ; Almajdoubi, Zaid ; M. Garbaj, Aboubaker ; M. Azwai, Salah ; T. Gammoudi, Fatim ; K. Abolghait, Said ; A. Moawad, Ashraf ; Barbieri, Ilaria ; L. Eshamah, Hanan ; M. Eldaghayes, Ibrahim</creator><creatorcontrib>T. Naas, Hesham ; Almajdoubi, Zaid ; M. Garbaj, Aboubaker ; M. Azwai, Salah ; T. Gammoudi, Fatim ; K. Abolghait, Said ; A. Moawad, Ashraf ; Barbieri, Ilaria ; L. Eshamah, Hanan ; M. Eldaghayes, Ibrahim</creatorcontrib><description>This study was conducted to investigate the presence of Enterococcus spp. in meat, meat products and seafood. A hundred and four samples were randomly collected from different geographic localities in Libya. The samples were subjected to microbiological analysis for enumeration and isolation of Enterococcus spp. by conventional cultural and molecular identification using PCR and partial sequencing of 16S rDNA techniques. Out of 104 samples, 73 (70.2%) isolates were found to be enterococci based on their cultural characteristics on ESD medium. However, out of 36 samples subjected to molecular identification, only six isolates were confirmed to be Enterococcus spp. using PCR and partial sequencing of 16S rDNA technique. All enterococci strains tested for their antibiotic sensitivity profiles showed high percentage of multi-resistance phenotype. These results can be used for further studies on enterococci as an emerging food borne pathogen and its role in human infection in Libya and would suggest that meat, meat products and seafood might play a role in the spreading of enterococci through the food chain with antimicrobial resistance characteristics.</description><identifier>ISSN: 1338-5178</identifier><identifier>EISSN: 1338-5178</identifier><identifier>DOI: 10.15414/jmbfs.2017.6.6.1264-1268</identifier><language>eng</language><publisher>Nitra: Faculty of Biotechnology and Food Sciences</publisher><subject>Antibiotics ; Antimicrobial resistance ; Cultural characteristics ; Enumeration ; Food chains ; Food processing industry ; Infections ; Meat ; Meat products ; Microbiological analysis ; Pathogens ; Polymerase chain reaction ; rRNA 16S ; Seafood ; Selective media</subject><ispartof>Journal of microbiology, biotechnology and food sciences, 2017-06, Vol.6 (6), p.1264-1268</ispartof><rights>Copyright Faculty of Biotechnology and Food Sciences Jun/Jul 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-83fc7585335bacea72d5f2169e443dfbe41de0ec2e45911fc6d8fc72f2665c263</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>T. 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Naas, Hesham ; Almajdoubi, Zaid ; M. Garbaj, Aboubaker ; M. Azwai, Salah ; T. Gammoudi, Fatim ; K. Abolghait, Said ; A. Moawad, Ashraf ; Barbieri, Ilaria ; L. Eshamah, Hanan ; M. Eldaghayes, Ibrahim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-83fc7585335bacea72d5f2169e443dfbe41de0ec2e45911fc6d8fc72f2665c263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Antibiotics</topic><topic>Antimicrobial resistance</topic><topic>Cultural characteristics</topic><topic>Enumeration</topic><topic>Food chains</topic><topic>Food processing industry</topic><topic>Infections</topic><topic>Meat</topic><topic>Meat products</topic><topic>Microbiological analysis</topic><topic>Pathogens</topic><topic>Polymerase chain reaction</topic><topic>rRNA 16S</topic><topic>Seafood</topic><topic>Selective media</topic><toplevel>online_resources</toplevel><creatorcontrib>T. 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Naas, Hesham</au><au>Almajdoubi, Zaid</au><au>M. Garbaj, Aboubaker</au><au>M. Azwai, Salah</au><au>T. Gammoudi, Fatim</au><au>K. Abolghait, Said</au><au>A. Moawad, Ashraf</au><au>Barbieri, Ilaria</au><au>L. Eshamah, Hanan</au><au>M. Eldaghayes, Ibrahim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MOLECULAR IDENTIFICATION AND ANTIBIOGRAM OF Enterococcus spp. ISOLATED ON ENTEROCOCCUS SELECTIVE DIFFERENTIAL (ESD) MEDIA FROM MEAT, MEAT PRODUCTS AND SEAFOOD IN LIBYA</atitle><jtitle>Journal of microbiology, biotechnology and food sciences</jtitle><date>2017-06-01</date><risdate>2017</risdate><volume>6</volume><issue>6</issue><spage>1264</spage><epage>1268</epage><pages>1264-1268</pages><issn>1338-5178</issn><eissn>1338-5178</eissn><abstract>This study was conducted to investigate the presence of Enterococcus spp. in meat, meat products and seafood. A hundred and four samples were randomly collected from different geographic localities in Libya. 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subjects | Antibiotics Antimicrobial resistance Cultural characteristics Enumeration Food chains Food processing industry Infections Meat Meat products Microbiological analysis Pathogens Polymerase chain reaction rRNA 16S Seafood Selective media |
title | MOLECULAR IDENTIFICATION AND ANTIBIOGRAM OF Enterococcus spp. ISOLATED ON ENTEROCOCCUS SELECTIVE DIFFERENTIAL (ESD) MEDIA FROM MEAT, MEAT PRODUCTS AND SEAFOOD IN LIBYA |
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