MOLECULAR IDENTIFICATION AND ANTIBIOGRAM OF Enterococcus spp. ISOLATED ON ENTEROCOCCUS SELECTIVE DIFFERENTIAL (ESD) MEDIA FROM MEAT, MEAT PRODUCTS AND SEAFOOD IN LIBYA

This study was conducted to investigate the presence of Enterococcus spp. in meat, meat products and seafood. A hundred and four samples were randomly collected from different geographic localities in Libya. The samples were subjected to microbiological analysis for enumeration and isolation of Ente...

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Veröffentlicht in:Journal of microbiology, biotechnology and food sciences biotechnology and food sciences, 2017-06, Vol.6 (6), p.1264-1268
Hauptverfasser: T. Naas, Hesham, Almajdoubi, Zaid, M. Garbaj, Aboubaker, M. Azwai, Salah, T. Gammoudi, Fatim, K. Abolghait, Said, A. Moawad, Ashraf, Barbieri, Ilaria, L. Eshamah, Hanan, M. Eldaghayes, Ibrahim
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container_issue 6
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container_title Journal of microbiology, biotechnology and food sciences
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creator T. Naas, Hesham
Almajdoubi, Zaid
M. Garbaj, Aboubaker
M. Azwai, Salah
T. Gammoudi, Fatim
K. Abolghait, Said
A. Moawad, Ashraf
Barbieri, Ilaria
L. Eshamah, Hanan
M. Eldaghayes, Ibrahim
description This study was conducted to investigate the presence of Enterococcus spp. in meat, meat products and seafood. A hundred and four samples were randomly collected from different geographic localities in Libya. The samples were subjected to microbiological analysis for enumeration and isolation of Enterococcus spp. by conventional cultural and molecular identification using PCR and partial sequencing of 16S rDNA techniques. Out of 104 samples, 73 (70.2%) isolates were found to be enterococci based on their cultural characteristics on ESD medium. However, out of 36 samples subjected to molecular identification, only six isolates were confirmed to be Enterococcus spp. using PCR and partial sequencing of 16S rDNA technique. All enterococci strains tested for their antibiotic sensitivity profiles showed high percentage of multi-resistance phenotype. These results can be used for further studies on enterococci as an emerging food borne pathogen and its role in human infection in Libya and would suggest that meat, meat products and seafood might play a role in the spreading of enterococci through the food chain with antimicrobial resistance characteristics.
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Naas, Hesham ; Almajdoubi, Zaid ; M. Garbaj, Aboubaker ; M. Azwai, Salah ; T. Gammoudi, Fatim ; K. Abolghait, Said ; A. Moawad, Ashraf ; Barbieri, Ilaria ; L. Eshamah, Hanan ; M. Eldaghayes, Ibrahim</creator><creatorcontrib>T. Naas, Hesham ; Almajdoubi, Zaid ; M. Garbaj, Aboubaker ; M. Azwai, Salah ; T. Gammoudi, Fatim ; K. Abolghait, Said ; A. Moawad, Ashraf ; Barbieri, Ilaria ; L. Eshamah, Hanan ; M. Eldaghayes, Ibrahim</creatorcontrib><description>This study was conducted to investigate the presence of Enterococcus spp. in meat, meat products and seafood. A hundred and four samples were randomly collected from different geographic localities in Libya. The samples were subjected to microbiological analysis for enumeration and isolation of Enterococcus spp. by conventional cultural and molecular identification using PCR and partial sequencing of 16S rDNA techniques. Out of 104 samples, 73 (70.2%) isolates were found to be enterococci based on their cultural characteristics on ESD medium. However, out of 36 samples subjected to molecular identification, only six isolates were confirmed to be Enterococcus spp. using PCR and partial sequencing of 16S rDNA technique. All enterococci strains tested for their antibiotic sensitivity profiles showed high percentage of multi-resistance phenotype. 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subjects Antibiotics
Antimicrobial resistance
Cultural characteristics
Enumeration
Food chains
Food processing industry
Infections
Meat
Meat products
Microbiological analysis
Pathogens
Polymerase chain reaction
rRNA 16S
Seafood
Selective media
title MOLECULAR IDENTIFICATION AND ANTIBIOGRAM OF Enterococcus spp. ISOLATED ON ENTEROCOCCUS SELECTIVE DIFFERENTIAL (ESD) MEDIA FROM MEAT, MEAT PRODUCTS AND SEAFOOD IN LIBYA
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