PREPARATION OF PLANT VECTOR CONSTRUCT CONTAINING DEHYDRIN GENE At2g21490
This work is focused on preparation of a plant transformation vector pDH2 containing the expression unit of the dehydrin gene At2g21490 under a control of the constitutive dCaMV 35S promoter and the selectable marker neomycin phosphotransferase gene controlled by the nos promoter. The gene At2g21490...
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| Veröffentlicht in: | Journal of microbiology, biotechnology and food sciences biotechnology and food sciences, 2017-06, Vol.6 (6), p.1261-1263 |
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| container_title | Journal of microbiology, biotechnology and food sciences |
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| creator | Zimová, Mária Boszorádová, Eva Gregorová, Zuzana Matušíková, Ildikó Moravčíková, Jana |
| description | This work is focused on preparation of a plant transformation vector pDH2 containing the expression unit of the dehydrin gene At2g21490 under a control of the constitutive dCaMV 35S promoter and the selectable marker neomycin phosphotransferase gene controlled by the nos promoter. The gene At2g21490 is classified as a dehydrin of the type K2SY2. The gene was isolated from Arabidopsis thaliana by PCR approach. The plasmid pDH2 was transformed into the strain Agrobacterium tumefaciens LBA 4404. The stability of the plasmid pDH2 in Agrobacterium has been verified by restriction analysis after re-transformation of the pDH2 into Escherichia coli. |
| doi_str_mv | 10.15414/jmbfs.2017.6.6.1261-1263 |
| format | Article |
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The gene At2g21490 is classified as a dehydrin of the type K2SY2. The gene was isolated from Arabidopsis thaliana by PCR approach. The plasmid pDH2 was transformed into the strain Agrobacterium tumefaciens LBA 4404. The stability of the plasmid pDH2 in Agrobacterium has been verified by restriction analysis after re-transformation of the pDH2 into Escherichia coli.</description><identifier>ISSN: 1338-5178</identifier><identifier>EISSN: 1338-5178</identifier><identifier>DOI: 10.15414/jmbfs.2017.6.6.1261-1263</identifier><language>eng</language><publisher>Nitra: Faculty of Biotechnology and Food Sciences</publisher><subject>Construction planning ; Dehydrin ; E coli ; Expression vectors ; Gene expression ; Genetic transformation ; Neomycin ; Neomycin phosphotransferase ; Phosphotransferase ; Phosphotransferase gene ; Polymerase chain reaction</subject><ispartof>Journal of microbiology, biotechnology and food sciences, 2017-06, Vol.6 (6), p.1261-1263</ispartof><rights>Copyright Faculty of Biotechnology and Food Sciences Jun/Jul 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Zimová, Mária</creatorcontrib><creatorcontrib>Boszorádová, Eva</creatorcontrib><creatorcontrib>Gregorová, Zuzana</creatorcontrib><creatorcontrib>Matušíková, Ildikó</creatorcontrib><creatorcontrib>Moravčíková, Jana</creatorcontrib><title>PREPARATION OF PLANT VECTOR CONSTRUCT CONTAINING DEHYDRIN GENE At2g21490</title><title>Journal of microbiology, biotechnology and food sciences</title><description>This work is focused on preparation of a plant transformation vector pDH2 containing the expression unit of the dehydrin gene At2g21490 under a control of the constitutive dCaMV 35S promoter and the selectable marker neomycin phosphotransferase gene controlled by the nos promoter. The gene At2g21490 is classified as a dehydrin of the type K2SY2. The gene was isolated from Arabidopsis thaliana by PCR approach. The plasmid pDH2 was transformed into the strain Agrobacterium tumefaciens LBA 4404. The stability of the plasmid pDH2 in Agrobacterium has been verified by restriction analysis after re-transformation of the pDH2 into Escherichia coli.</description><subject>Construction planning</subject><subject>Dehydrin</subject><subject>E coli</subject><subject>Expression vectors</subject><subject>Gene expression</subject><subject>Genetic transformation</subject><subject>Neomycin</subject><subject>Neomycin phosphotransferase</subject><subject>Phosphotransferase</subject><subject>Phosphotransferase gene</subject><subject>Polymerase chain reaction</subject><issn>1338-5178</issn><issn>1338-5178</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpNUMtqwzAQFKWFhjT_oNKzU60etnQ0jpMYghxcpdCTsFW5NDRNaieH_H3lpIfuwOzAzu7CIPQIZAqCA3_e7pq2n1ICyTQOABpDFIjdoBEwJiMBibz9p-_RpO-3JFSsEirECC3XVb5Oq9QUpcblHK9XqTb4Nc9MWeGs1C-m2mRmUCYtdKEXeJYv32ZVofEi1zlOj_SDAlfkAd219VfvJ399jDbz3GTLaFUuiixdRY5KfowUZYmopecevCNNrTxvWsWAwjsThFAKsUg4947WDaOcOe-dUA4ck2FBARujp-vdQ7f_Ofn-aLf7U_cdXloIY6BcKhlc6upy3b7vO9_aQ_e5q7uzBWIv2dlLdnbIzsYBQ3YDMfYLzQxb4Q</recordid><startdate>20170601</startdate><enddate>20170601</enddate><creator>Zimová, Mária</creator><creator>Boszorádová, Eva</creator><creator>Gregorová, Zuzana</creator><creator>Matušíková, Ildikó</creator><creator>Moravčíková, Jana</creator><general>Faculty of Biotechnology and Food Sciences</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope></search><sort><creationdate>20170601</creationdate><title>PREPARATION OF PLANT VECTOR CONSTRUCT CONTAINING DEHYDRIN GENE At2g21490</title><author>Zimová, Mária ; 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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Construction planning Dehydrin E coli Expression vectors Gene expression Genetic transformation Neomycin Neomycin phosphotransferase Phosphotransferase Phosphotransferase gene Polymerase chain reaction |
| title | PREPARATION OF PLANT VECTOR CONSTRUCT CONTAINING DEHYDRIN GENE At2g21490 |
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