Oral administration of transgenic barley expressing a Culicoides allergen induces specific antibody response
Summary Background Insect bite hypersensitivity is an immunoglobulin (Ig)E‐mediated dermatitis of horses initiated by bites of midges of the genus Culicoides. Culicoides spp. are not indigenous to Iceland and the prevalence of insect bite hypersensitivity is much higher in horses born in Iceland and...
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| Veröffentlicht in: | Equine veterinary journal 2017-07, Vol.49 (4), p.512-518 |
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| container_title | Equine veterinary journal |
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| creator | Jonsdottir, S. Svansson, V. Stefansdottir, S. B. Mäntylä, E. Marti, E. Torsteinsdottir, S. |
| description | Summary
Background
Insect bite hypersensitivity is an immunoglobulin (Ig)E‐mediated dermatitis of horses initiated by bites of midges of the genus Culicoides. Culicoides spp. are not indigenous to Iceland and the prevalence of insect bite hypersensitivity is much higher in horses born in Iceland and exported as compared to Icelandic horses born in a Culicoides rich environment. Immunotherapy is therefore needed.
Objectives
The aim of the study was to express an allergen from Culicoides in barley grain and investigate whether an immune response could be obtained in healthy Icelandic horses by oral treatment with transgenic barley expressing the allergen.
Study design
In vivo experiment.
Methods
The allergen was expressed in barley grain with the Orfeus technique. A device was developed to treat horses orally with barley flour. Four Icelandic horses were treated with transgenic barley and 3 with control barley, in total 500 g in 7 feedings. Serum and saliva samples were collected for measuring specific antibodies.
Results
The allergen Cul n 2, a hyaluronidase originating from the salivary gland of Culicoides nubeculosus, was expressed in barley. Horses treated with the transgenic barley mounted a Cul n 2 specific IgG1 and IgG4/7 response in serum and saliva. The serum response was significantly different between the transgenic and control barley treated horses for both subclasses and the saliva response for IgG1. The induced serum antibodies bound to the corresponding allergen from Culicoides obsoletus, rCul o 2 and were able to partially block binding of Cul n 2 as well as Cul o 2 specific IgE from insect bite hypersensitivity affected horses.
Main limitations
Small number of horses.
Conclusion
This study shows that specific antibody response can be induced in horses not exposed to Culicoides, using oral treatment with transgenic barley expressing an allergen. Further studies will determine whether this approach is a useful alternative for prevention and treatment of equine insect bite hypersensitivity. |
| doi_str_mv | 10.1111/evj.12655 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1908242729</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1908242729</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4215-309a866be69db4b4f0cfdd4a42b91f8b31913f0b6a25a2278c648591b625fcfd3</originalsourceid><addsrcrecordid>eNp1kUFPXCEUhUmjqaPton-gIXFTF0-BB_hYNhO1NSZutFsCPDBMGHiFedr5914d20UT7-bekO-ecM9B6AslpxTqzD-uTimTQnxAC0Y46_qeyD20gFF0VHJ-gA5bWxHS94yzj-iAnQ9CiYEvULqtJmEzrmOObVPNJpaMS8Aw5vbgc3TYmpr8Fvs_U_WtxfyADV7OKboSR9-wSclXIHHM4-zgoU3exQCLJm-iLeMWw95UcvOf0H4wqfnPb_0I3V9e3C1_dDe3Vz-X3286xxkVXU-UGaS0XqrRcssDcWEcueHMKhoG21NF-0CsNEwYBrc4yeEeaiUTAdD-CH3b6U61_J592-h1bM6nZLIvc9OMEMKVolwAevwfuipzzfA7TRUZwK5zpoA62VGultaqD3qqcW3qVlOiXyLQEIF-jQDYr2-Ks1378R_513MAznbAUwRf31fSF7-ud5LPKviRXQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1908242729</pqid></control><display><type>article</type><title>Oral administration of transgenic barley expressing a Culicoides allergen induces specific antibody response</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Jonsdottir, S. ; Svansson, V. ; Stefansdottir, S. B. ; Mäntylä, E. ; Marti, E. ; Torsteinsdottir, S.</creator><creatorcontrib>Jonsdottir, S. ; Svansson, V. ; Stefansdottir, S. B. ; Mäntylä, E. ; Marti, E. ; Torsteinsdottir, S.</creatorcontrib><description>Summary
Background
Insect bite hypersensitivity is an immunoglobulin (Ig)E‐mediated dermatitis of horses initiated by bites of midges of the genus Culicoides. Culicoides spp. are not indigenous to Iceland and the prevalence of insect bite hypersensitivity is much higher in horses born in Iceland and exported as compared to Icelandic horses born in a Culicoides rich environment. Immunotherapy is therefore needed.
Objectives
The aim of the study was to express an allergen from Culicoides in barley grain and investigate whether an immune response could be obtained in healthy Icelandic horses by oral treatment with transgenic barley expressing the allergen.
Study design
In vivo experiment.
Methods
The allergen was expressed in barley grain with the Orfeus technique. A device was developed to treat horses orally with barley flour. Four Icelandic horses were treated with transgenic barley and 3 with control barley, in total 500 g in 7 feedings. Serum and saliva samples were collected for measuring specific antibodies.
Results
The allergen Cul n 2, a hyaluronidase originating from the salivary gland of Culicoides nubeculosus, was expressed in barley. Horses treated with the transgenic barley mounted a Cul n 2 specific IgG1 and IgG4/7 response in serum and saliva. The serum response was significantly different between the transgenic and control barley treated horses for both subclasses and the saliva response for IgG1. The induced serum antibodies bound to the corresponding allergen from Culicoides obsoletus, rCul o 2 and were able to partially block binding of Cul n 2 as well as Cul o 2 specific IgE from insect bite hypersensitivity affected horses.
Main limitations
Small number of horses.
Conclusion
This study shows that specific antibody response can be induced in horses not exposed to Culicoides, using oral treatment with transgenic barley expressing an allergen. Further studies will determine whether this approach is a useful alternative for prevention and treatment of equine insect bite hypersensitivity.</description><identifier>ISSN: 0425-1644</identifier><identifier>EISSN: 2042-3306</identifier><identifier>DOI: 10.1111/evj.12655</identifier><identifier>PMID: 27859584</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Administration, Oral ; allergens ; Allergens - immunology ; Animals ; antibodies ; Antibody Formation ; Antibody response ; Barley ; barley flour ; barley grain ; Bites ; blood serum ; Ceratopogonidae - immunology ; Culicoides ; Culicoides allergen ; Culicoides nubeculosus ; Culicoides obsoletus ; Dermatitis ; Design ; Environments ; Exposure ; Flour ; Grain ; Hordeum - genetics ; horse ; Horse Diseases - immunology ; Horses ; hyaluronoglucosaminidase ; Hypersensitivity ; Hypersensitivity - veterinary ; Iceland ; Icelandic (horse breed) ; Immune response ; Immunoglobulin E ; Immunoglobulin G ; Immunotherapy ; in vivo studies ; insect bite hypersensitivity ; Insect bites ; Insect Bites and Stings - immunology ; Insect Bites and Stings - veterinary ; midges ; Oral administration ; Oral cavity ; oral immunotherapy ; Saliva ; Salivary gland ; salivary glands ; transgenic plants</subject><ispartof>Equine veterinary journal, 2017-07, Vol.49 (4), p.512-518</ispartof><rights>2016 EVJ Ltd</rights><rights>2016 EVJ Ltd.</rights><rights>2017 EVJ Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4215-309a866be69db4b4f0cfdd4a42b91f8b31913f0b6a25a2278c648591b625fcfd3</citedby><cites>FETCH-LOGICAL-c4215-309a866be69db4b4f0cfdd4a42b91f8b31913f0b6a25a2278c648591b625fcfd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fevj.12655$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fevj.12655$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27859584$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jonsdottir, S.</creatorcontrib><creatorcontrib>Svansson, V.</creatorcontrib><creatorcontrib>Stefansdottir, S. B.</creatorcontrib><creatorcontrib>Mäntylä, E.</creatorcontrib><creatorcontrib>Marti, E.</creatorcontrib><creatorcontrib>Torsteinsdottir, S.</creatorcontrib><title>Oral administration of transgenic barley expressing a Culicoides allergen induces specific antibody response</title><title>Equine veterinary journal</title><addtitle>Equine Vet J</addtitle><description>Summary
Background
Insect bite hypersensitivity is an immunoglobulin (Ig)E‐mediated dermatitis of horses initiated by bites of midges of the genus Culicoides. Culicoides spp. are not indigenous to Iceland and the prevalence of insect bite hypersensitivity is much higher in horses born in Iceland and exported as compared to Icelandic horses born in a Culicoides rich environment. Immunotherapy is therefore needed.
Objectives
The aim of the study was to express an allergen from Culicoides in barley grain and investigate whether an immune response could be obtained in healthy Icelandic horses by oral treatment with transgenic barley expressing the allergen.
Study design
In vivo experiment.
Methods
The allergen was expressed in barley grain with the Orfeus technique. A device was developed to treat horses orally with barley flour. Four Icelandic horses were treated with transgenic barley and 3 with control barley, in total 500 g in 7 feedings. Serum and saliva samples were collected for measuring specific antibodies.
Results
The allergen Cul n 2, a hyaluronidase originating from the salivary gland of Culicoides nubeculosus, was expressed in barley. Horses treated with the transgenic barley mounted a Cul n 2 specific IgG1 and IgG4/7 response in serum and saliva. The serum response was significantly different between the transgenic and control barley treated horses for both subclasses and the saliva response for IgG1. The induced serum antibodies bound to the corresponding allergen from Culicoides obsoletus, rCul o 2 and were able to partially block binding of Cul n 2 as well as Cul o 2 specific IgE from insect bite hypersensitivity affected horses.
Main limitations
Small number of horses.
Conclusion
This study shows that specific antibody response can be induced in horses not exposed to Culicoides, using oral treatment with transgenic barley expressing an allergen. Further studies will determine whether this approach is a useful alternative for prevention and treatment of equine insect bite hypersensitivity.</description><subject>Administration, Oral</subject><subject>allergens</subject><subject>Allergens - immunology</subject><subject>Animals</subject><subject>antibodies</subject><subject>Antibody Formation</subject><subject>Antibody response</subject><subject>Barley</subject><subject>barley flour</subject><subject>barley grain</subject><subject>Bites</subject><subject>blood serum</subject><subject>Ceratopogonidae - immunology</subject><subject>Culicoides</subject><subject>Culicoides allergen</subject><subject>Culicoides nubeculosus</subject><subject>Culicoides obsoletus</subject><subject>Dermatitis</subject><subject>Design</subject><subject>Environments</subject><subject>Exposure</subject><subject>Flour</subject><subject>Grain</subject><subject>Hordeum - genetics</subject><subject>horse</subject><subject>Horse Diseases - immunology</subject><subject>Horses</subject><subject>hyaluronoglucosaminidase</subject><subject>Hypersensitivity</subject><subject>Hypersensitivity - veterinary</subject><subject>Iceland</subject><subject>Icelandic (horse breed)</subject><subject>Immune response</subject><subject>Immunoglobulin E</subject><subject>Immunoglobulin G</subject><subject>Immunotherapy</subject><subject>in vivo studies</subject><subject>insect bite hypersensitivity</subject><subject>Insect bites</subject><subject>Insect Bites and Stings - immunology</subject><subject>Insect Bites and Stings - veterinary</subject><subject>midges</subject><subject>Oral administration</subject><subject>Oral cavity</subject><subject>oral immunotherapy</subject><subject>Saliva</subject><subject>Salivary gland</subject><subject>salivary glands</subject><subject>transgenic plants</subject><issn>0425-1644</issn><issn>2042-3306</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUFPXCEUhUmjqaPton-gIXFTF0-BB_hYNhO1NSZutFsCPDBMGHiFedr5914d20UT7-bekO-ecM9B6AslpxTqzD-uTimTQnxAC0Y46_qeyD20gFF0VHJ-gA5bWxHS94yzj-iAnQ9CiYEvULqtJmEzrmOObVPNJpaMS8Aw5vbgc3TYmpr8Fvs_U_WtxfyADV7OKboSR9-wSclXIHHM4-zgoU3exQCLJm-iLeMWw95UcvOf0H4wqfnPb_0I3V9e3C1_dDe3Vz-X3286xxkVXU-UGaS0XqrRcssDcWEcueHMKhoG21NF-0CsNEwYBrc4yeEeaiUTAdD-CH3b6U61_J592-h1bM6nZLIvc9OMEMKVolwAevwfuipzzfA7TRUZwK5zpoA62VGultaqD3qqcW3qVlOiXyLQEIF-jQDYr2-Ks1378R_513MAznbAUwRf31fSF7-ud5LPKviRXQ</recordid><startdate>201707</startdate><enddate>201707</enddate><creator>Jonsdottir, S.</creator><creator>Svansson, V.</creator><creator>Stefansdottir, S. B.</creator><creator>Mäntylä, E.</creator><creator>Marti, E.</creator><creator>Torsteinsdottir, S.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>201707</creationdate><title>Oral administration of transgenic barley expressing a Culicoides allergen induces specific antibody response</title><author>Jonsdottir, S. ; Svansson, V. ; Stefansdottir, S. B. ; Mäntylä, E. ; Marti, E. ; Torsteinsdottir, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4215-309a866be69db4b4f0cfdd4a42b91f8b31913f0b6a25a2278c648591b625fcfd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Administration, Oral</topic><topic>allergens</topic><topic>Allergens - immunology</topic><topic>Animals</topic><topic>antibodies</topic><topic>Antibody Formation</topic><topic>Antibody response</topic><topic>Barley</topic><topic>barley flour</topic><topic>barley grain</topic><topic>Bites</topic><topic>blood serum</topic><topic>Ceratopogonidae - immunology</topic><topic>Culicoides</topic><topic>Culicoides allergen</topic><topic>Culicoides nubeculosus</topic><topic>Culicoides obsoletus</topic><topic>Dermatitis</topic><topic>Design</topic><topic>Environments</topic><topic>Exposure</topic><topic>Flour</topic><topic>Grain</topic><topic>Hordeum - genetics</topic><topic>horse</topic><topic>Horse Diseases - immunology</topic><topic>Horses</topic><topic>hyaluronoglucosaminidase</topic><topic>Hypersensitivity</topic><topic>Hypersensitivity - veterinary</topic><topic>Iceland</topic><topic>Icelandic (horse breed)</topic><topic>Immune response</topic><topic>Immunoglobulin E</topic><topic>Immunoglobulin G</topic><topic>Immunotherapy</topic><topic>in vivo studies</topic><topic>insect bite hypersensitivity</topic><topic>Insect bites</topic><topic>Insect Bites and Stings - immunology</topic><topic>Insect Bites and Stings - veterinary</topic><topic>midges</topic><topic>Oral administration</topic><topic>Oral cavity</topic><topic>oral immunotherapy</topic><topic>Saliva</topic><topic>Salivary gland</topic><topic>salivary glands</topic><topic>transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jonsdottir, S.</creatorcontrib><creatorcontrib>Svansson, V.</creatorcontrib><creatorcontrib>Stefansdottir, S. B.</creatorcontrib><creatorcontrib>Mäntylä, E.</creatorcontrib><creatorcontrib>Marti, E.</creatorcontrib><creatorcontrib>Torsteinsdottir, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Equine veterinary journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jonsdottir, S.</au><au>Svansson, V.</au><au>Stefansdottir, S. B.</au><au>Mäntylä, E.</au><au>Marti, E.</au><au>Torsteinsdottir, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oral administration of transgenic barley expressing a Culicoides allergen induces specific antibody response</atitle><jtitle>Equine veterinary journal</jtitle><addtitle>Equine Vet J</addtitle><date>2017-07</date><risdate>2017</risdate><volume>49</volume><issue>4</issue><spage>512</spage><epage>518</epage><pages>512-518</pages><issn>0425-1644</issn><eissn>2042-3306</eissn><abstract>Summary
Background
Insect bite hypersensitivity is an immunoglobulin (Ig)E‐mediated dermatitis of horses initiated by bites of midges of the genus Culicoides. Culicoides spp. are not indigenous to Iceland and the prevalence of insect bite hypersensitivity is much higher in horses born in Iceland and exported as compared to Icelandic horses born in a Culicoides rich environment. Immunotherapy is therefore needed.
Objectives
The aim of the study was to express an allergen from Culicoides in barley grain and investigate whether an immune response could be obtained in healthy Icelandic horses by oral treatment with transgenic barley expressing the allergen.
Study design
In vivo experiment.
Methods
The allergen was expressed in barley grain with the Orfeus technique. A device was developed to treat horses orally with barley flour. Four Icelandic horses were treated with transgenic barley and 3 with control barley, in total 500 g in 7 feedings. Serum and saliva samples were collected for measuring specific antibodies.
Results
The allergen Cul n 2, a hyaluronidase originating from the salivary gland of Culicoides nubeculosus, was expressed in barley. Horses treated with the transgenic barley mounted a Cul n 2 specific IgG1 and IgG4/7 response in serum and saliva. The serum response was significantly different between the transgenic and control barley treated horses for both subclasses and the saliva response for IgG1. The induced serum antibodies bound to the corresponding allergen from Culicoides obsoletus, rCul o 2 and were able to partially block binding of Cul n 2 as well as Cul o 2 specific IgE from insect bite hypersensitivity affected horses.
Main limitations
Small number of horses.
Conclusion
This study shows that specific antibody response can be induced in horses not exposed to Culicoides, using oral treatment with transgenic barley expressing an allergen. Further studies will determine whether this approach is a useful alternative for prevention and treatment of equine insect bite hypersensitivity.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>27859584</pmid><doi>10.1111/evj.12655</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Equine veterinary journal, 2017-07, Vol.49 (4), p.512-518 |
| issn | 0425-1644 2042-3306 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Administration, Oral allergens Allergens - immunology Animals antibodies Antibody Formation Antibody response Barley barley flour barley grain Bites blood serum Ceratopogonidae - immunology Culicoides Culicoides allergen Culicoides nubeculosus Culicoides obsoletus Dermatitis Design Environments Exposure Flour Grain Hordeum - genetics horse Horse Diseases - immunology Horses hyaluronoglucosaminidase Hypersensitivity Hypersensitivity - veterinary Iceland Icelandic (horse breed) Immune response Immunoglobulin E Immunoglobulin G Immunotherapy in vivo studies insect bite hypersensitivity Insect bites Insect Bites and Stings - immunology Insect Bites and Stings - veterinary midges Oral administration Oral cavity oral immunotherapy Saliva Salivary gland salivary glands transgenic plants |
| title | Oral administration of transgenic barley expressing a Culicoides allergen induces specific antibody response |
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