OP0119 The CRL4 Cereblon E3 Ubiquitin Ligase Modulator CC-220 Induces Degradation of the Transcription Factors Aiolos and Ikaros: Immunomodulation in Healthy Volunteers and Relevance to Systemic Lupus Erythematosus
BackgroundCC-220 is an immunomodulatory compound that binds to cereblon (CRBN), part of the CRL4CRBN E3 ubiquitin ligase complex, which has been shown to ubiquitinate the transcription factors Ikaros (IKZF1) and Aiolos (IKZF3). Polymorphisms at the IKZF1 and IKZF3 loci have been associated with risk...
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Veröffentlicht in: | Annals of the rheumatic diseases 2015-06, Vol.74 (Suppl 2), p.113-113 |
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description | BackgroundCC-220 is an immunomodulatory compound that binds to cereblon (CRBN), part of the CRL4CRBN E3 ubiquitin ligase complex, which has been shown to ubiquitinate the transcription factors Ikaros (IKZF1) and Aiolos (IKZF3). Polymorphisms at the IKZF1 and IKZF3 loci have been associated with risk of systemic lupus erythematosus (SLE).ObjectivesWe explored CRBN, IKZF1, and IKZF3 gene expression in peripheral blood mononuclear cells (PBMC) from SLE patients; the effect of CC-220 on Ikaros and Aiolos protein levels and SLE autoantibody production in vitro; and the impact of CC-220 on immunological parameters in a double-blinded, placebo-controlled, single-ascending dose, healthy volunteer phase 1 clinical trial.MethodsCRBN, IKZF1, and IKZF3 gene expression were measured by qRT-PCR. Ikaros and Aiolos protein levels were measured by western blot and flow cytometry. Anti-dsDNA and anti-phospholipid autoantibodies were measured from SLE PBMC cultures treated for 7 days with CC-220. In the phase 1 healthy volunteer study, 56 subjects were randomized and enrolled in 7 cohorts, with 6 subjects per cohort receiving a single oral dose of CC-220 (0.03-6 mg) and 2 subjects per cohort receiving placebo. CD19+ B cells, CD3+ T cells, and intracellular Aiolos were measured by flow cytometry. IL-2 and IL-1β production were stimulated with anti-CD3 or LPS, respectively, in the TruCulture ex vivo whole blood assay system.ResultsCompared to normal PBMC, SLE PBMC expressed significantly higher levels of CRBN (1.5-fold), IKZF1 (2.1-fold), and IKZF3 (4.1-fold). CC-220 treatment of whole blood significantly reduced Aiolos and Ikaros protein levels in B cells, T cells, and monocytes, but not in granulocytes. In cultures of SLE PBMC, CC-220 inhibited anti-dsDNA and anti-phospholipid autoantibody production with an IC50 of $≈ $10 nM. Following administration of single doses of CC-220 to healthy volunteers, there was a treatment-related decrease in intracellular Aiolos, with minimum mean percent of baseline values of $≈ $12%>28% in B cells and 0%>33% in T cells, for 0.3-6 mg. There was also a treatment-related decrease in absolute CD19+ B cells and CD3+ T cells, with minimum mean percent of baseline values of $≈ $41%>67% for B cells and 66%>73% for T cells, for 2-6 mg. CC-220 administration also resulted in increased IL-2 (maximum mean percent of baseline values ranging from 247%>1896% for 0.1-6 mg), and a decrease in IL-1β (minimum mean percent of baseline values of $≈ $11% at 6 mg |
doi_str_mv | 10.1136/annrheumdis-2015-eular.3498 |
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fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1901812195</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4322508787</sourcerecordid><originalsourceid>FETCH-LOGICAL-b1855-acb8a32e4cc0e4e8c0680e76904e4635f01553592dee62910ddbaf3182bc5e8f3</originalsourceid><addsrcrecordid>eNqVkc1u1DAUhS0EEkPhHa7UdYqdH9eBVRWmdKSgojJlaznOTcdDYk_tGGl2bHhH1jwJnoYFW1aWr853zrUPIeeMXjBW8LfKWr_DOPUmZDllVYZxVP6iKGvxjKxYyUUac_qcrCilRVbW_PIleRXCPl2pYGJFft1-pozVv3_83O4Qmru2hAY9dqOzsC7gvjOP0czGQmseVED45PoUMTsPTZPlOYWN7aPGAB_wwatezSaBboA5uW29skF7c3gaXiudsABXxo0ugLI9bL4p78I72ExTtG5arE_alHeDapx3R_jqxmhnRL8gdzjid2U1wuzgyzHMOBkNbTzEAGt_TLFT2i7E8Jq8GNQY8M3f84zcX6-3zU3W3n7cNFdt1jFRVZnSnVBFjqXWFEsUmnJB8ZLXtMSSF9WQfrUqqjrvEXleM9r3nRoKJvJOVyiG4oycL74H7x4jhlnuXfQ2RUpWUyZYzuoqqd4vKp0eHDwO8uDNpPxRMipPVcp_qpSnKuVTlfJUZaL5QnfT_r_APz-TrnY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1901812195</pqid></control><display><type>article</type><title>OP0119 The CRL4 Cereblon E3 Ubiquitin Ligase Modulator CC-220 Induces Degradation of the Transcription Factors Aiolos and Ikaros: Immunomodulation in Healthy Volunteers and Relevance to Systemic Lupus Erythematosus</title><source>BMJ Journals - NESLi2</source><creator>Schafer, P. ; Ye, Y. ; Wu, L. ; Kosek, J. ; Yang, Z. ; Liu, L. ; Thomas, M. ; Palmisano, M. ; Chopra, R.</creator><creatorcontrib>Schafer, P. ; Ye, Y. ; Wu, L. ; Kosek, J. ; Yang, Z. ; Liu, L. ; Thomas, M. ; Palmisano, M. ; Chopra, R.</creatorcontrib><description>BackgroundCC-220 is an immunomodulatory compound that binds to cereblon (CRBN), part of the CRL4CRBN E3 ubiquitin ligase complex, which has been shown to ubiquitinate the transcription factors Ikaros (IKZF1) and Aiolos (IKZF3). Polymorphisms at the IKZF1 and IKZF3 loci have been associated with risk of systemic lupus erythematosus (SLE).ObjectivesWe explored CRBN, IKZF1, and IKZF3 gene expression in peripheral blood mononuclear cells (PBMC) from SLE patients; the effect of CC-220 on Ikaros and Aiolos protein levels and SLE autoantibody production in vitro; and the impact of CC-220 on immunological parameters in a double-blinded, placebo-controlled, single-ascending dose, healthy volunteer phase 1 clinical trial.MethodsCRBN, IKZF1, and IKZF3 gene expression were measured by qRT-PCR. Ikaros and Aiolos protein levels were measured by western blot and flow cytometry. Anti-dsDNA and anti-phospholipid autoantibodies were measured from SLE PBMC cultures treated for 7 days with CC-220. In the phase 1 healthy volunteer study, 56 subjects were randomized and enrolled in 7 cohorts, with 6 subjects per cohort receiving a single oral dose of CC-220 (0.03-6 mg) and 2 subjects per cohort receiving placebo. CD19+ B cells, CD3+ T cells, and intracellular Aiolos were measured by flow cytometry. IL-2 and IL-1β production were stimulated with anti-CD3 or LPS, respectively, in the TruCulture ex vivo whole blood assay system.ResultsCompared to normal PBMC, SLE PBMC expressed significantly higher levels of CRBN (1.5-fold), IKZF1 (2.1-fold), and IKZF3 (4.1-fold). CC-220 treatment of whole blood significantly reduced Aiolos and Ikaros protein levels in B cells, T cells, and monocytes, but not in granulocytes. In cultures of SLE PBMC, CC-220 inhibited anti-dsDNA and anti-phospholipid autoantibody production with an IC50 of $≈ $10 nM. Following administration of single doses of CC-220 to healthy volunteers, there was a treatment-related decrease in intracellular Aiolos, with minimum mean percent of baseline values of $≈ $12%>28% in B cells and 0%>33% in T cells, for 0.3-6 mg. There was also a treatment-related decrease in absolute CD19+ B cells and CD3+ T cells, with minimum mean percent of baseline values of $≈ $41%>67% for B cells and 66%>73% for T cells, for 2-6 mg. CC-220 administration also resulted in increased IL-2 (maximum mean percent of baseline values ranging from 247%>1896% for 0.1-6 mg), and a decrease in IL-1β (minimum mean percent of baseline values of $≈ $11% at 6 mg).ConclusionsThese results demonstrate that CRBN, IKZF1, and IKZF3 mRNA are overexpressed in PBMC of SLE patients. Targeting the CRL4CRBN E3 ubiquitin ligase with CC-220 resulted in a potent reduction in Aiolos and Ikaros protein levels in B cells, T cells, and monocytes, and inhibited autoantibody production. Administration of single doses of CC-220 (0.3-6 mg) reduced intracellular Aiolos protein expression in B cells and T cells, reduced absolute B-cell and T-cell counts in peripheral blood, and increased T-cell-derived IL-2 production and decreased LPS-induced IL-1β production in whole blood ex vivo. These findings support the further development of CC-220 for the treatment of SLE and other autoimmune diseases.Disclosure of InterestP. Schafer Employee of: Celgene Corporation, Y. Ye Employee of: Celgene Corporation, L. Wu Employee of: Celgene Corporation, J. Kosek Employee of: Celgene Corporation, Z. Yang Employee of: Celgene Corporation, L. Liu Employee of: Celgene Corporation, M. Thomas Employee of: Celgene Corporation, M. Palmisano Employee of: Celgene Corporation, R. Chopra Employee of: Celgene Corporation</description><identifier>ISSN: 0003-4967</identifier><identifier>EISSN: 1468-2060</identifier><identifier>DOI: 10.1136/annrheumdis-2015-eular.3498</identifier><identifier>CODEN: ARDIAO</identifier><language>eng</language><publisher>London: BMJ Publishing Group LTD</publisher><ispartof>Annals of the rheumatic diseases, 2015-06, Vol.74 (Suppl 2), p.113-113</ispartof><rights>2015, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions</rights><rights>Copyright: 2015 (c) 2015, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b1855-acb8a32e4cc0e4e8c0680e76904e4635f01553592dee62910ddbaf3182bc5e8f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttp://ard.bmj.com/content/74/Suppl_2/113.2.full.pdf$$EPDF$$P50$$Gbmj$$H</linktopdf><linktohtml>$$Uhttp://ard.bmj.com/content/74/Suppl_2/113.2.full$$EHTML$$P50$$Gbmj$$H</linktohtml><link.rule.ids>114,115,314,780,784,3196,23571,27924,27925,77600,77631</link.rule.ids></links><search><creatorcontrib>Schafer, P.</creatorcontrib><creatorcontrib>Ye, Y.</creatorcontrib><creatorcontrib>Wu, L.</creatorcontrib><creatorcontrib>Kosek, J.</creatorcontrib><creatorcontrib>Yang, Z.</creatorcontrib><creatorcontrib>Liu, L.</creatorcontrib><creatorcontrib>Thomas, M.</creatorcontrib><creatorcontrib>Palmisano, M.</creatorcontrib><creatorcontrib>Chopra, R.</creatorcontrib><title>OP0119 The CRL4 Cereblon E3 Ubiquitin Ligase Modulator CC-220 Induces Degradation of the Transcription Factors Aiolos and Ikaros: Immunomodulation in Healthy Volunteers and Relevance to Systemic Lupus Erythematosus</title><title>Annals of the rheumatic diseases</title><description>BackgroundCC-220 is an immunomodulatory compound that binds to cereblon (CRBN), part of the CRL4CRBN E3 ubiquitin ligase complex, which has been shown to ubiquitinate the transcription factors Ikaros (IKZF1) and Aiolos (IKZF3). Polymorphisms at the IKZF1 and IKZF3 loci have been associated with risk of systemic lupus erythematosus (SLE).ObjectivesWe explored CRBN, IKZF1, and IKZF3 gene expression in peripheral blood mononuclear cells (PBMC) from SLE patients; the effect of CC-220 on Ikaros and Aiolos protein levels and SLE autoantibody production in vitro; and the impact of CC-220 on immunological parameters in a double-blinded, placebo-controlled, single-ascending dose, healthy volunteer phase 1 clinical trial.MethodsCRBN, IKZF1, and IKZF3 gene expression were measured by qRT-PCR. Ikaros and Aiolos protein levels were measured by western blot and flow cytometry. Anti-dsDNA and anti-phospholipid autoantibodies were measured from SLE PBMC cultures treated for 7 days with CC-220. In the phase 1 healthy volunteer study, 56 subjects were randomized and enrolled in 7 cohorts, with 6 subjects per cohort receiving a single oral dose of CC-220 (0.03-6 mg) and 2 subjects per cohort receiving placebo. CD19+ B cells, CD3+ T cells, and intracellular Aiolos were measured by flow cytometry. IL-2 and IL-1β production were stimulated with anti-CD3 or LPS, respectively, in the TruCulture ex vivo whole blood assay system.ResultsCompared to normal PBMC, SLE PBMC expressed significantly higher levels of CRBN (1.5-fold), IKZF1 (2.1-fold), and IKZF3 (4.1-fold). CC-220 treatment of whole blood significantly reduced Aiolos and Ikaros protein levels in B cells, T cells, and monocytes, but not in granulocytes. In cultures of SLE PBMC, CC-220 inhibited anti-dsDNA and anti-phospholipid autoantibody production with an IC50 of $≈ $10 nM. Following administration of single doses of CC-220 to healthy volunteers, there was a treatment-related decrease in intracellular Aiolos, with minimum mean percent of baseline values of $≈ $12%>28% in B cells and 0%>33% in T cells, for 0.3-6 mg. There was also a treatment-related decrease in absolute CD19+ B cells and CD3+ T cells, with minimum mean percent of baseline values of $≈ $41%>67% for B cells and 66%>73% for T cells, for 2-6 mg. CC-220 administration also resulted in increased IL-2 (maximum mean percent of baseline values ranging from 247%>1896% for 0.1-6 mg), and a decrease in IL-1β (minimum mean percent of baseline values of $≈ $11% at 6 mg).ConclusionsThese results demonstrate that CRBN, IKZF1, and IKZF3 mRNA are overexpressed in PBMC of SLE patients. Targeting the CRL4CRBN E3 ubiquitin ligase with CC-220 resulted in a potent reduction in Aiolos and Ikaros protein levels in B cells, T cells, and monocytes, and inhibited autoantibody production. Administration of single doses of CC-220 (0.3-6 mg) reduced intracellular Aiolos protein expression in B cells and T cells, reduced absolute B-cell and T-cell counts in peripheral blood, and increased T-cell-derived IL-2 production and decreased LPS-induced IL-1β production in whole blood ex vivo. These findings support the further development of CC-220 for the treatment of SLE and other autoimmune diseases.Disclosure of InterestP. Schafer Employee of: Celgene Corporation, Y. Ye Employee of: Celgene Corporation, L. Wu Employee of: Celgene Corporation, J. Kosek Employee of: Celgene Corporation, Z. Yang Employee of: Celgene Corporation, L. Liu Employee of: Celgene Corporation, M. Thomas Employee of: Celgene Corporation, M. Palmisano Employee of: Celgene Corporation, R. Chopra Employee of: Celgene Corporation</description><issn>0003-4967</issn><issn>1468-2060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkc1u1DAUhS0EEkPhHa7UdYqdH9eBVRWmdKSgojJlaznOTcdDYk_tGGl2bHhH1jwJnoYFW1aWr853zrUPIeeMXjBW8LfKWr_DOPUmZDllVYZxVP6iKGvxjKxYyUUac_qcrCilRVbW_PIleRXCPl2pYGJFft1-pozVv3_83O4Qmru2hAY9dqOzsC7gvjOP0czGQmseVED45PoUMTsPTZPlOYWN7aPGAB_wwatezSaBboA5uW29skF7c3gaXiudsABXxo0ugLI9bL4p78I72ExTtG5arE_alHeDapx3R_jqxmhnRL8gdzjid2U1wuzgyzHMOBkNbTzEAGt_TLFT2i7E8Jq8GNQY8M3f84zcX6-3zU3W3n7cNFdt1jFRVZnSnVBFjqXWFEsUmnJB8ZLXtMSSF9WQfrUqqjrvEXleM9r3nRoKJvJOVyiG4oycL74H7x4jhlnuXfQ2RUpWUyZYzuoqqd4vKp0eHDwO8uDNpPxRMipPVcp_qpSnKuVTlfJUZaL5QnfT_r_APz-TrnY</recordid><startdate>201506</startdate><enddate>201506</enddate><creator>Schafer, P.</creator><creator>Ye, Y.</creator><creator>Wu, L.</creator><creator>Kosek, J.</creator><creator>Yang, Z.</creator><creator>Liu, L.</creator><creator>Thomas, M.</creator><creator>Palmisano, M.</creator><creator>Chopra, R.</creator><general>BMJ Publishing Group LTD</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>201506</creationdate><title>OP0119 The CRL4 Cereblon E3 Ubiquitin Ligase Modulator CC-220 Induces Degradation of the Transcription Factors Aiolos and Ikaros: Immunomodulation in Healthy Volunteers and Relevance to Systemic Lupus Erythematosus</title><author>Schafer, P. ; Ye, Y. ; Wu, L. ; Kosek, J. ; Yang, Z. ; Liu, L. ; Thomas, M. ; Palmisano, M. ; Chopra, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b1855-acb8a32e4cc0e4e8c0680e76904e4635f01553592dee62910ddbaf3182bc5e8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schafer, P.</creatorcontrib><creatorcontrib>Ye, Y.</creatorcontrib><creatorcontrib>Wu, L.</creatorcontrib><creatorcontrib>Kosek, J.</creatorcontrib><creatorcontrib>Yang, Z.</creatorcontrib><creatorcontrib>Liu, L.</creatorcontrib><creatorcontrib>Thomas, M.</creatorcontrib><creatorcontrib>Palmisano, M.</creatorcontrib><creatorcontrib>Chopra, R.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Annals of the rheumatic diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schafer, P.</au><au>Ye, Y.</au><au>Wu, L.</au><au>Kosek, J.</au><au>Yang, Z.</au><au>Liu, L.</au><au>Thomas, M.</au><au>Palmisano, M.</au><au>Chopra, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>OP0119 The CRL4 Cereblon E3 Ubiquitin Ligase Modulator CC-220 Induces Degradation of the Transcription Factors Aiolos and Ikaros: Immunomodulation in Healthy Volunteers and Relevance to Systemic Lupus Erythematosus</atitle><jtitle>Annals of the rheumatic diseases</jtitle><date>2015-06</date><risdate>2015</risdate><volume>74</volume><issue>Suppl 2</issue><spage>113</spage><epage>113</epage><pages>113-113</pages><issn>0003-4967</issn><eissn>1468-2060</eissn><coden>ARDIAO</coden><abstract>BackgroundCC-220 is an immunomodulatory compound that binds to cereblon (CRBN), part of the CRL4CRBN E3 ubiquitin ligase complex, which has been shown to ubiquitinate the transcription factors Ikaros (IKZF1) and Aiolos (IKZF3). Polymorphisms at the IKZF1 and IKZF3 loci have been associated with risk of systemic lupus erythematosus (SLE).ObjectivesWe explored CRBN, IKZF1, and IKZF3 gene expression in peripheral blood mononuclear cells (PBMC) from SLE patients; the effect of CC-220 on Ikaros and Aiolos protein levels and SLE autoantibody production in vitro; and the impact of CC-220 on immunological parameters in a double-blinded, placebo-controlled, single-ascending dose, healthy volunteer phase 1 clinical trial.MethodsCRBN, IKZF1, and IKZF3 gene expression were measured by qRT-PCR. Ikaros and Aiolos protein levels were measured by western blot and flow cytometry. Anti-dsDNA and anti-phospholipid autoantibodies were measured from SLE PBMC cultures treated for 7 days with CC-220. In the phase 1 healthy volunteer study, 56 subjects were randomized and enrolled in 7 cohorts, with 6 subjects per cohort receiving a single oral dose of CC-220 (0.03-6 mg) and 2 subjects per cohort receiving placebo. CD19+ B cells, CD3+ T cells, and intracellular Aiolos were measured by flow cytometry. IL-2 and IL-1β production were stimulated with anti-CD3 or LPS, respectively, in the TruCulture ex vivo whole blood assay system.ResultsCompared to normal PBMC, SLE PBMC expressed significantly higher levels of CRBN (1.5-fold), IKZF1 (2.1-fold), and IKZF3 (4.1-fold). CC-220 treatment of whole blood significantly reduced Aiolos and Ikaros protein levels in B cells, T cells, and monocytes, but not in granulocytes. In cultures of SLE PBMC, CC-220 inhibited anti-dsDNA and anti-phospholipid autoantibody production with an IC50 of $≈ $10 nM. Following administration of single doses of CC-220 to healthy volunteers, there was a treatment-related decrease in intracellular Aiolos, with minimum mean percent of baseline values of $≈ $12%>28% in B cells and 0%>33% in T cells, for 0.3-6 mg. There was also a treatment-related decrease in absolute CD19+ B cells and CD3+ T cells, with minimum mean percent of baseline values of $≈ $41%>67% for B cells and 66%>73% for T cells, for 2-6 mg. CC-220 administration also resulted in increased IL-2 (maximum mean percent of baseline values ranging from 247%>1896% for 0.1-6 mg), and a decrease in IL-1β (minimum mean percent of baseline values of $≈ $11% at 6 mg).ConclusionsThese results demonstrate that CRBN, IKZF1, and IKZF3 mRNA are overexpressed in PBMC of SLE patients. Targeting the CRL4CRBN E3 ubiquitin ligase with CC-220 resulted in a potent reduction in Aiolos and Ikaros protein levels in B cells, T cells, and monocytes, and inhibited autoantibody production. Administration of single doses of CC-220 (0.3-6 mg) reduced intracellular Aiolos protein expression in B cells and T cells, reduced absolute B-cell and T-cell counts in peripheral blood, and increased T-cell-derived IL-2 production and decreased LPS-induced IL-1β production in whole blood ex vivo. These findings support the further development of CC-220 for the treatment of SLE and other autoimmune diseases.Disclosure of InterestP. Schafer Employee of: Celgene Corporation, Y. Ye Employee of: Celgene Corporation, L. Wu Employee of: Celgene Corporation, J. Kosek Employee of: Celgene Corporation, Z. Yang Employee of: Celgene Corporation, L. Liu Employee of: Celgene Corporation, M. Thomas Employee of: Celgene Corporation, M. Palmisano Employee of: Celgene Corporation, R. Chopra Employee of: Celgene Corporation</abstract><cop>London</cop><pub>BMJ Publishing Group LTD</pub><doi>10.1136/annrheumdis-2015-eular.3498</doi><tpages>1</tpages></addata></record> |
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source | BMJ Journals - NESLi2 |
title | OP0119 The CRL4 Cereblon E3 Ubiquitin Ligase Modulator CC-220 Induces Degradation of the Transcription Factors Aiolos and Ikaros: Immunomodulation in Healthy Volunteers and Relevance to Systemic Lupus Erythematosus |
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