Ultrasound-assisted extraction of fucoidan from Sargassum muticum
Ultrasound-assisted aqueous extraction (USAE) of crude fucoidan from Sargassum muticum using an ultrasonic bath (150 W and 40 kHz ) at room temperature (25 °C) for 5–30 min was studied. Fucoidan-containing extracts were separated by centrifugation and characterized by HPLC, HPSEC, FT-IR, and MALDI-T...
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| Veröffentlicht in: | Journal of applied phycology 2017-06, Vol.29 (3), p.1553-1561 |
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| container_title | Journal of applied phycology |
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| creator | Flórez-Fernández, Noelia López-García, Marta González-Muñoz, María Jesús Vilariño, José Manuel López Domínguez, Herminia |
| description | Ultrasound-assisted aqueous extraction (USAE) of crude fucoidan from
Sargassum muticum
using an ultrasonic bath (150 W and 40 kHz ) at room temperature (25 °C) for 5–30 min was studied. Fucoidan-containing extracts were separated by centrifugation and characterized by HPLC, HPSEC, FT-IR, and MALDI-TOF spectrometry. Crude fucoidan on the best conditions was composed by oligosaccharides of fucose:galactose:glucose:xylose:mannose:rhamnose in a mass ratio of 1:0.70:0.33:0.27:0.22:0.10. The maximum sulfate content was 39.54 ± 0.001-mg sulfate ion g
−1
extract. Furthermore, extract contained 25-mg phlorotannins g
−1
extract and showed an ABTS radical scavenging activity equivalent to 40-mg Trolox g
−1
extract measured with the Trolox Equivalent Antioxidant Capacity (TEAC) assay. The cell viability of selected tumoral human cell lines was reduced up to 30% in the presence of crude extracts at 500 mg L
−1
. |
| doi_str_mv | 10.1007/s10811-016-1043-9 |
| format | Article |
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Sargassum muticum
using an ultrasonic bath (150 W and 40 kHz ) at room temperature (25 °C) for 5–30 min was studied. Fucoidan-containing extracts were separated by centrifugation and characterized by HPLC, HPSEC, FT-IR, and MALDI-TOF spectrometry. Crude fucoidan on the best conditions was composed by oligosaccharides of fucose:galactose:glucose:xylose:mannose:rhamnose in a mass ratio of 1:0.70:0.33:0.27:0.22:0.10. The maximum sulfate content was 39.54 ± 0.001-mg sulfate ion g
−1
extract. Furthermore, extract contained 25-mg phlorotannins g
−1
extract and showed an ABTS radical scavenging activity equivalent to 40-mg Trolox g
−1
extract measured with the Trolox Equivalent Antioxidant Capacity (TEAC) assay. The cell viability of selected tumoral human cell lines was reduced up to 30% in the presence of crude extracts at 500 mg L
−1
.</description><identifier>ISSN: 0921-8971</identifier><identifier>EISSN: 1573-5176</identifier><identifier>DOI: 10.1007/s10811-016-1043-9</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antioxidants ; Biomedical and Life Sciences ; Biotechnology ; Capacity ; Cell lines ; Centrifugation ; Centrifuging ; Ecology ; Equivalence ; Extraction ; Freshwater & Marine Ecology ; Fucoidan ; Fucose ; Galactose ; High-performance liquid chromatography ; HPLC ; Infrared spectroscopy ; Life Sciences ; Lines ; Liquid chromatography ; Mannose ; Oligosaccharides ; Plant Physiology ; Plant Sciences ; Rhamnose ; Scavenging ; Spectrometry ; Sulfates ; Temperature effects ; Ultrasonic imaging ; Ultrasound ; Vitamin E ; Xylose</subject><ispartof>Journal of applied phycology, 2017-06, Vol.29 (3), p.1553-1561</ispartof><rights>Springer Science+Business Media Dordrecht 2017</rights><rights>Journal of Applied Phycology is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-2e63af7c5d39ce62fac21b2ad5f92dc4f6f765b34b437042a8314cc20df518b33</citedby><cites>FETCH-LOGICAL-c316t-2e63af7c5d39ce62fac21b2ad5f92dc4f6f765b34b437042a8314cc20df518b33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10811-016-1043-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10811-016-1043-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Flórez-Fernández, Noelia</creatorcontrib><creatorcontrib>López-García, Marta</creatorcontrib><creatorcontrib>González-Muñoz, María Jesús</creatorcontrib><creatorcontrib>Vilariño, José Manuel López</creatorcontrib><creatorcontrib>Domínguez, Herminia</creatorcontrib><title>Ultrasound-assisted extraction of fucoidan from Sargassum muticum</title><title>Journal of applied phycology</title><addtitle>J Appl Phycol</addtitle><description>Ultrasound-assisted aqueous extraction (USAE) of crude fucoidan from
Sargassum muticum
using an ultrasonic bath (150 W and 40 kHz ) at room temperature (25 °C) for 5–30 min was studied. Fucoidan-containing extracts were separated by centrifugation and characterized by HPLC, HPSEC, FT-IR, and MALDI-TOF spectrometry. Crude fucoidan on the best conditions was composed by oligosaccharides of fucose:galactose:glucose:xylose:mannose:rhamnose in a mass ratio of 1:0.70:0.33:0.27:0.22:0.10. The maximum sulfate content was 39.54 ± 0.001-mg sulfate ion g
−1
extract. Furthermore, extract contained 25-mg phlorotannins g
−1
extract and showed an ABTS radical scavenging activity equivalent to 40-mg Trolox g
−1
extract measured with the Trolox Equivalent Antioxidant Capacity (TEAC) assay. The cell viability of selected tumoral human cell lines was reduced up to 30% in the presence of crude extracts at 500 mg L
−1
.</description><subject>Antioxidants</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Capacity</subject><subject>Cell lines</subject><subject>Centrifugation</subject><subject>Centrifuging</subject><subject>Ecology</subject><subject>Equivalence</subject><subject>Extraction</subject><subject>Freshwater & Marine Ecology</subject><subject>Fucoidan</subject><subject>Fucose</subject><subject>Galactose</subject><subject>High-performance liquid chromatography</subject><subject>HPLC</subject><subject>Infrared spectroscopy</subject><subject>Life Sciences</subject><subject>Lines</subject><subject>Liquid chromatography</subject><subject>Mannose</subject><subject>Oligosaccharides</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Rhamnose</subject><subject>Scavenging</subject><subject>Spectrometry</subject><subject>Sulfates</subject><subject>Temperature effects</subject><subject>Ultrasonic imaging</subject><subject>Ultrasound</subject><subject>Vitamin E</subject><subject>Xylose</subject><issn>0921-8971</issn><issn>1573-5176</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kEtLAzEUhYMoWKs_wN2A62huksljWYovKLjQrkMmk5QpnUlNJqD_3injwo2rC4fvnAsfQrdA7oEQ-ZCBKABMQGAgnGF9hhZQS4ZrkOIcLYimgJWWcImuct4TQrQCtUCr7WFMNscytNjm3OXRt5X_mjI3dnGoYqhCcbFr7VCFFPvq3abdBJa-6svYudJfo4tgD9nf_N4l2j49fqxf8Obt-XW92mDHQIyYesFskK5umXZe0GAdhYbatg6ato4HEaSoG8YbziTh1CoG3DlK2lCDahhbort595jiZ_F5NPtY0jC9NKC0FporeaJgplyKOScfzDF1vU3fBog5mTKzKTOZMidTRk8dOnfyxA47n_4s_1v6AXeQa-4</recordid><startdate>20170601</startdate><enddate>20170601</enddate><creator>Flórez-Fernández, Noelia</creator><creator>López-García, Marta</creator><creator>González-Muñoz, María Jesús</creator><creator>Vilariño, José Manuel López</creator><creator>Domínguez, Herminia</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TN</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>GNUQQ</scope><scope>H95</scope><scope>HCIFZ</scope><scope>L.G</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20170601</creationdate><title>Ultrasound-assisted extraction of fucoidan from Sargassum muticum</title><author>Flórez-Fernández, Noelia ; 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Sargassum muticum
using an ultrasonic bath (150 W and 40 kHz ) at room temperature (25 °C) for 5–30 min was studied. Fucoidan-containing extracts were separated by centrifugation and characterized by HPLC, HPSEC, FT-IR, and MALDI-TOF spectrometry. Crude fucoidan on the best conditions was composed by oligosaccharides of fucose:galactose:glucose:xylose:mannose:rhamnose in a mass ratio of 1:0.70:0.33:0.27:0.22:0.10. The maximum sulfate content was 39.54 ± 0.001-mg sulfate ion g
−1
extract. Furthermore, extract contained 25-mg phlorotannins g
−1
extract and showed an ABTS radical scavenging activity equivalent to 40-mg Trolox g
−1
extract measured with the Trolox Equivalent Antioxidant Capacity (TEAC) assay. The cell viability of selected tumoral human cell lines was reduced up to 30% in the presence of crude extracts at 500 mg L
−1
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10811-016-1043-9</doi><tpages>9</tpages></addata></record> |
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| subjects | Antioxidants Biomedical and Life Sciences Biotechnology Capacity Cell lines Centrifugation Centrifuging Ecology Equivalence Extraction Freshwater & Marine Ecology Fucoidan Fucose Galactose High-performance liquid chromatography HPLC Infrared spectroscopy Life Sciences Lines Liquid chromatography Mannose Oligosaccharides Plant Physiology Plant Sciences Rhamnose Scavenging Spectrometry Sulfates Temperature effects Ultrasonic imaging Ultrasound Vitamin E Xylose |
| title | Ultrasound-assisted extraction of fucoidan from Sargassum muticum |
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