Genistein inhibited ammonia induced astrocyte swelling by inhibiting NF-[kappa]B activation-mediated nitric oxide formation
Our previous study has indicated the involvement of epidermal growth factor receptor (EGFR) transactivation in ammonia-induced astrocyte swelling, which represents a major pathogenesis of brain edema in hepatic encephalopathy. In this study, we examined the effect of genistein, a naturally occurred...
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| Veröffentlicht in: | Metabolic brain disease 2017-06, Vol.32 (3), p.841 |
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| creator | Dai, Hongliang Jia, Guizhi Wang, Wei Liang, Chunguang Han, Siyu Chu, Minghui Mei, Xifan |
| description | Our previous study has indicated the involvement of epidermal growth factor receptor (EGFR) transactivation in ammonia-induced astrocyte swelling, which represents a major pathogenesis of brain edema in hepatic encephalopathy. In this study, we examined the effect of genistein, a naturally occurred broad-spectrum protein tyrosine kinase (PTK) inhibitor, on ammonia-induced cell swelling. We found that genistein pretreatment significantly prevented ammonia-induced astrocyte swelling. Mechanistically, ammonia triggered EGFR/extracellular signal-regulated kinase (ERK) association and subsequent ERK phosphorylation were alleviated by genistein pretreatment. Moreover, ammonia-induced NF-[kappa]B nuclear location, iNOS expression, and consequent NO production were all prevented by AG1478 and genistein pretreatment. This study suggested that genistein could alleviate ammonia-induced astrocyte swelling, which may be, at least partly, related to its PTK-inhibiting activity and repression of NF-[kappa]B mediated iNOS-derived NO accumulation. |
| doi_str_mv | 10.1007/s11011-017-9975-6 |
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| subjects | Activation analysis Ammonia Brain Cell size Edema Enzyme inhibitors Epidermal growth factor Epidermal growth factor receptors Extracellular signal-regulated kinase Genistein Hepatic encephalopathy NF-κB protein Nitric oxide Nitric-oxide synthase Pathogenesis Phosphorylation Protein-tyrosine kinase Swelling Tyrosine |
| title | Genistein inhibited ammonia induced astrocyte swelling by inhibiting NF-[kappa]B activation-mediated nitric oxide formation |
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