Periostin Upregulates Wnt/[beta]-Catenin Signaling to Promote the Osteogenesis of CTLA4-Modified Human Bone Marrow-Mesenchymal Stem Cells
The enhanced osteogenesis of mesenchymal stem cells (MSCs) modified by expression of cytotoxic T lymphocyte-associated antigen 4 (CTLA4) has been shown in previous studies, but the mechanism remains unknown. Here we found that the bone repair effect of CTLA4-modified MSCs in demineralized bone matri...
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| Veröffentlicht in: | Scientific reports 2017-01, Vol.7, p.41634 |
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| creator | Zhang, Fei Luo, Keyu Rong, Zhigang Wang, Zhengdong Luo, Fei Zhang, Zehua Sun, Dong Dong, Shiwu Xu, Jianzhong Dai, Fei |
| description | The enhanced osteogenesis of mesenchymal stem cells (MSCs) modified by expression of cytotoxic T lymphocyte-associated antigen 4 (CTLA4) has been shown in previous studies, but the mechanism remains unknown. Here we found that the bone repair effect of CTLA4-modified MSCs in demineralized bone matrix (DBM) in a rabbit radius defect model was significantly better than that observed for unmodified MSCs in DBM or DBM alone, and the periostin (POSTN) expression in CTLA4-modified MSCs was significantly higher than that in unmodified MSCs both in vivo and in vitro. In addition, we also found that treatment of CTLA4-modified MSCs with soluble POSTN could inhibit the glycogen synthase kinase-3β activity and increase β-catenin expression through up-regulation of lipoprotein-related protein-6 phosphorylation to promote osteogenic differentiation, but blocking of integrin αvβ3, a receptor of POSTN, could suppress these effects. Our data demonstrated that POSTN expressed in response to CTLA4 can promote the osteogenesis of xenotransplanted MSCs through interaction with Wnt/β-catenin pathway. |
| doi_str_mv | 10.1038/srep41634 |
| format | Article |
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Here we found that the bone repair effect of CTLA4-modified MSCs in demineralized bone matrix (DBM) in a rabbit radius defect model was significantly better than that observed for unmodified MSCs in DBM or DBM alone, and the periostin (POSTN) expression in CTLA4-modified MSCs was significantly higher than that in unmodified MSCs both in vivo and in vitro. In addition, we also found that treatment of CTLA4-modified MSCs with soluble POSTN could inhibit the glycogen synthase kinase-3β activity and increase β-catenin expression through up-regulation of lipoprotein-related protein-6 phosphorylation to promote osteogenic differentiation, but blocking of integrin αvβ3, a receptor of POSTN, could suppress these effects. Our data demonstrated that POSTN expressed in response to CTLA4 can promote the osteogenesis of xenotransplanted MSCs through interaction with Wnt/β-catenin pathway.</description><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep41634</identifier><language>eng</language><publisher>London: Nature Publishing Group</publisher><subject>Bone healing ; Bone marrow ; Bone matrix ; CTLA-4 protein ; Cytotoxicity ; Glycogen ; Glycogen synthase kinase 3 ; Kinases ; Lymphocytes ; Mesenchymal stem cells ; Mesenchyme ; Osteogenesis ; Phosphorylation ; Radius ; Skin & tissue grafts ; Stem cells ; Wnt protein ; Xenografts ; β-Catenin</subject><ispartof>Scientific reports, 2017-01, Vol.7, p.41634</ispartof><rights>Copyright Nature Publishing Group Jan 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,865,27929,27930</link.rule.ids></links><search><creatorcontrib>Zhang, Fei</creatorcontrib><creatorcontrib>Luo, Keyu</creatorcontrib><creatorcontrib>Rong, Zhigang</creatorcontrib><creatorcontrib>Wang, Zhengdong</creatorcontrib><creatorcontrib>Luo, Fei</creatorcontrib><creatorcontrib>Zhang, Zehua</creatorcontrib><creatorcontrib>Sun, Dong</creatorcontrib><creatorcontrib>Dong, Shiwu</creatorcontrib><creatorcontrib>Xu, Jianzhong</creatorcontrib><creatorcontrib>Dai, Fei</creatorcontrib><title>Periostin Upregulates Wnt/[beta]-Catenin Signaling to Promote the Osteogenesis of CTLA4-Modified Human Bone Marrow-Mesenchymal Stem Cells</title><title>Scientific reports</title><description>The enhanced osteogenesis of mesenchymal stem cells (MSCs) modified by expression of cytotoxic T lymphocyte-associated antigen 4 (CTLA4) has been shown in previous studies, but the mechanism remains unknown. 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Our data demonstrated that POSTN expressed in response to CTLA4 can promote the osteogenesis of xenotransplanted MSCs through interaction with Wnt/β-catenin pathway.</description><subject>Bone healing</subject><subject>Bone marrow</subject><subject>Bone matrix</subject><subject>CTLA-4 protein</subject><subject>Cytotoxicity</subject><subject>Glycogen</subject><subject>Glycogen synthase kinase 3</subject><subject>Kinases</subject><subject>Lymphocytes</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchyme</subject><subject>Osteogenesis</subject><subject>Phosphorylation</subject><subject>Radius</subject><subject>Skin & tissue grafts</subject><subject>Stem cells</subject><subject>Wnt protein</subject><subject>Xenografts</subject><subject>β-Catenin</subject><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNjsFKw0AURQdBsGgX_sED17GZSVKSpQalC0MLrbgQKaN9SadM5sV5L4if4F-bhR_g3Vw49yyuUtc6vdVpVi444pDrZZafqZlJ8yIxmTEXas58SqcUpsp1NVM_G4yOWFyA5yFiN3oryPASZPH6jmLfknoCYZq3rgvWu9CBEGwi9SQIckRYsyB1GJAdA7VQ757u8qShg2sdHmA19jbAPQWExsZIX0mDjOHj-N1bD1vBHmr0nq_UeWs94_yvL9XN48OuXiVDpM8RWfYnGuN0gfe6rKoircpsmf3P-gWJXFfK</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Zhang, Fei</creator><creator>Luo, Keyu</creator><creator>Rong, Zhigang</creator><creator>Wang, Zhengdong</creator><creator>Luo, Fei</creator><creator>Zhang, Zehua</creator><creator>Sun, Dong</creator><creator>Dong, Shiwu</creator><creator>Xu, Jianzhong</creator><creator>Dai, Fei</creator><general>Nature Publishing Group</general><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20170101</creationdate><title>Periostin Upregulates Wnt/[beta]-Catenin Signaling to Promote the Osteogenesis of CTLA4-Modified Human Bone Marrow-Mesenchymal Stem Cells</title><author>Zhang, Fei ; 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Here we found that the bone repair effect of CTLA4-modified MSCs in demineralized bone matrix (DBM) in a rabbit radius defect model was significantly better than that observed for unmodified MSCs in DBM or DBM alone, and the periostin (POSTN) expression in CTLA4-modified MSCs was significantly higher than that in unmodified MSCs both in vivo and in vitro. In addition, we also found that treatment of CTLA4-modified MSCs with soluble POSTN could inhibit the glycogen synthase kinase-3β activity and increase β-catenin expression through up-regulation of lipoprotein-related protein-6 phosphorylation to promote osteogenic differentiation, but blocking of integrin αvβ3, a receptor of POSTN, could suppress these effects. Our data demonstrated that POSTN expressed in response to CTLA4 can promote the osteogenesis of xenotransplanted MSCs through interaction with Wnt/β-catenin pathway.</abstract><cop>London</cop><pub>Nature Publishing Group</pub><doi>10.1038/srep41634</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Bone healing Bone marrow Bone matrix CTLA-4 protein Cytotoxicity Glycogen Glycogen synthase kinase 3 Kinases Lymphocytes Mesenchymal stem cells Mesenchyme Osteogenesis Phosphorylation Radius Skin & tissue grafts Stem cells Wnt protein Xenografts β-Catenin |
| title | Periostin Upregulates Wnt/[beta]-Catenin Signaling to Promote the Osteogenesis of CTLA4-Modified Human Bone Marrow-Mesenchymal Stem Cells |
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