The chemiluminescence immunoassay for aflatoxin B1 based on functionalized magnetic nanoparticles with two strategies of antigen probe immobilization

A rapid and sensitive chemiluminescence immunoassay (CLIA) based on magnetic nanoparticles (MNPs) was developed to detect aflatoxin B1 (AFB1), which is a potent carcinogen in nature. We prepared monodisperse MNPs (300 nm in diameter) according to the solvothermal synthesis reaction and the MNPs were...

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Veröffentlicht in:	Luminescence (Chichester, England) England), 2017-06, Vol.32 (4), p.661-665
Hauptverfasser:	Yao, Manwen, Wang, Linyu, Fang, Chengzhi
Format:	Artikel
Sprache:	eng
Schlagworte:	AFB1 detection Aflatoxin B1 Aflatoxin B1 - analysis Aflatoxin B1 - immunology Amination Antibodies Antibodies, Monoclonal - chemistry Antigens Antigens - chemistry Calibration Carboxylation Carcinogens Chemical synthesis Chemiluminescence chemiluminescent immunoassays (CLIA) Detection functionalization Immobilization Immunoassay Immunoassay - instrumentation Immunoassay - methods Immunoassays Inhibitory Concentration 50 Limit of Detection Luminescent Measurements magnetic nanoparticles Magnetite Nanoparticles - chemistry Methods Nanoparticles NSP–DMAE–NHS Propylamines - chemistry Reagents Sensitivity and Specificity Silanes - chemistry Silica Silicon dioxide Solid phases Solids
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