RNAi induced silencing of pathogenicity genes of Fusarium spp. for vascular wilt management in tomato
The necessity to develop new strategies for the control of Fusarium wilt of tomato signifies the identification of pathogencity genes and ascertaining their role to use them as molecular tools for fungicide development or to develop transgenics. Semi-quantitative gene expression studies have identif...
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| Veröffentlicht in: | Annals of microbiology 2017-05, Vol.67 (5), p.359-369 |
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| creator | Shanmugam, Veerubommu Sharma, Vandana Bharti, Poonam Jyoti, Poonam Yadav, Sudesh Kumar Aggarwal, Rashmi Jain, Shekhar |
| description | The necessity to develop new strategies for the control of
Fusarium
wilt of tomato signifies the identification of pathogencity genes and ascertaining their role to use them as molecular tools for fungicide development or to develop transgenics. Semi-quantitative gene expression studies have identified two pathogenicity genes,
FOW2
and
chsV
, reported as ZnII)2Cys6-type transcription regulator and class V chitin synthase, respectively, as potential ones for being secreted all the time. The roles of these genes in the pathogenicity of
Fusarium oxysporum
and
F. solani
have been established by RNA interference (RNAi)-induced silencing (knockdown). The silencing vector encoding hairpin RNA of each of the gene fragment was constructed in a two-step PCR-based cloning, and introduced into the fungal genomic DNA. Silencing of either of the genes resulted in less virulent fungal phenotypes with altered physiological characteristics like sporulation and growth on solid media and a reduction in mRNA expression. The results therefore demonstrate the applicability of these pathogenicity genes as useful molecular targets for exploitation in
Fusarium
wilt control in tomato. |
| doi_str_mv | 10.1007/s13213-017-1265-3 |
| format | Article |
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Fusarium
wilt of tomato signifies the identification of pathogencity genes and ascertaining their role to use them as molecular tools for fungicide development or to develop transgenics. Semi-quantitative gene expression studies have identified two pathogenicity genes,
FOW2
and
chsV
, reported as ZnII)2Cys6-type transcription regulator and class V chitin synthase, respectively, as potential ones for being secreted all the time. The roles of these genes in the pathogenicity of
Fusarium oxysporum
and
F. solani
have been established by RNA interference (RNAi)-induced silencing (knockdown). The silencing vector encoding hairpin RNA of each of the gene fragment was constructed in a two-step PCR-based cloning, and introduced into the fungal genomic DNA. Silencing of either of the genes resulted in less virulent fungal phenotypes with altered physiological characteristics like sporulation and growth on solid media and a reduction in mRNA expression. The results therefore demonstrate the applicability of these pathogenicity genes as useful molecular targets for exploitation in
Fusarium
wilt control in tomato.</description><identifier>ISSN: 1590-4261</identifier><identifier>EISSN: 1869-2044</identifier><identifier>DOI: 10.1007/s13213-017-1265-3</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Applied Microbiology ; Biomedical and Life Sciences ; Chitin ; Chitin synthase ; Fungicides ; Gene expression ; Gene silencing ; Genetic engineering ; Genetic transcription ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Mycology ; Original Article ; Pathogenicity ; Polymerase chain reaction ; RNA ; RNA-mediated interference ; Sporulation ; Transcription ; Virulence (Microbiology) ; Wilt</subject><ispartof>Annals of microbiology, 2017-05, Vol.67 (5), p.359-369</ispartof><rights>Springer-Verlag Berlin Heidelberg and the University of Milan 2017</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Copyright Springer Science & Business Media 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-6a55e4ed5d0f033c6f219c599a9c024d265c5c3d6635ce38f8ec64d21ef8723d3</citedby><cites>FETCH-LOGICAL-c389t-6a55e4ed5d0f033c6f219c599a9c024d265c5c3d6635ce38f8ec64d21ef8723d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13213-017-1265-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13213-017-1265-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41096,41464,42165,42533,51294,51551</link.rule.ids><linktorsrc>$$Uhttps://doi.org/10.1007/s13213-017-1265-3$$EView_record_in_Springer_Nature$$FView_record_in_$$GSpringer_Nature</linktorsrc></links><search><creatorcontrib>Shanmugam, Veerubommu</creatorcontrib><creatorcontrib>Sharma, Vandana</creatorcontrib><creatorcontrib>Bharti, Poonam</creatorcontrib><creatorcontrib>Jyoti, Poonam</creatorcontrib><creatorcontrib>Yadav, Sudesh Kumar</creatorcontrib><creatorcontrib>Aggarwal, Rashmi</creatorcontrib><creatorcontrib>Jain, Shekhar</creatorcontrib><title>RNAi induced silencing of pathogenicity genes of Fusarium spp. for vascular wilt management in tomato</title><title>Annals of microbiology</title><addtitle>Ann Microbiol</addtitle><description>The necessity to develop new strategies for the control of
Fusarium
wilt of tomato signifies the identification of pathogencity genes and ascertaining their role to use them as molecular tools for fungicide development or to develop transgenics. Semi-quantitative gene expression studies have identified two pathogenicity genes,
FOW2
and
chsV
, reported as ZnII)2Cys6-type transcription regulator and class V chitin synthase, respectively, as potential ones for being secreted all the time. The roles of these genes in the pathogenicity of
Fusarium oxysporum
and
F. solani
have been established by RNA interference (RNAi)-induced silencing (knockdown). The silencing vector encoding hairpin RNA of each of the gene fragment was constructed in a two-step PCR-based cloning, and introduced into the fungal genomic DNA. Silencing of either of the genes resulted in less virulent fungal phenotypes with altered physiological characteristics like sporulation and growth on solid media and a reduction in mRNA expression. The results therefore demonstrate the applicability of these pathogenicity genes as useful molecular targets for exploitation in
Fusarium
wilt control in tomato.</description><subject>Applied Microbiology</subject><subject>Biomedical and Life Sciences</subject><subject>Chitin</subject><subject>Chitin synthase</subject><subject>Fungicides</subject><subject>Gene expression</subject><subject>Gene silencing</subject><subject>Genetic engineering</subject><subject>Genetic transcription</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Original Article</subject><subject>Pathogenicity</subject><subject>Polymerase chain reaction</subject><subject>RNA</subject><subject>RNA-mediated interference</subject><subject>Sporulation</subject><subject>Transcription</subject><subject>Virulence (Microbiology)</subject><subject>Wilt</subject><issn>1590-4261</issn><issn>1869-2044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp1kctq3TAQhk1poLk9QHaCrrLwqWRdLC0PoblAaCFt10LII1fBlhxJbpq3jw7OolkULTQM3z-3v2kuCN4RjPsvmdCO0BaTviWd4C390BwTKVTbYcY-1pgr3LJOkE_NSc6PGAvFFDtu4OHb3iMfhtXCgLKfIFgfRhQdWkz5HUcI3vrygmoA-ZC-XrNJfp1RXpYdcjGhPybbdTIJPfupoNkEM8IModSyqMTZlHjWHDkzZTh_-0-bX9dff17dtvffb-6u9vetpVKVVhjOgcHAB-wwpVa4jijLlTLK4o4NdTHLLR2EoNwClU6CFTVNwMm-owM9bT5vdZcUn1bIRT_GNYXaUhOpBO4J47RSu40azQTaBxdLMra-AWZvYwBXz6D3vZRUSNWzKrh8J6hMgb9lNGvO-u7Hw3uWbKxNMecETi_Jzya9aIL1wSq9WaWrVfpglT4M1G2aXNkwQvpn7P-KXgEpTpW-</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Shanmugam, Veerubommu</creator><creator>Sharma, Vandana</creator><creator>Bharti, Poonam</creator><creator>Jyoti, Poonam</creator><creator>Yadav, Sudesh Kumar</creator><creator>Aggarwal, Rashmi</creator><creator>Jain, Shekhar</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope></search><sort><creationdate>20170501</creationdate><title>RNAi induced silencing of pathogenicity genes of Fusarium spp. for vascular wilt management in tomato</title><author>Shanmugam, Veerubommu ; Sharma, Vandana ; Bharti, Poonam ; Jyoti, Poonam ; Yadav, Sudesh Kumar ; Aggarwal, Rashmi ; Jain, Shekhar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-6a55e4ed5d0f033c6f219c599a9c024d265c5c3d6635ce38f8ec64d21ef8723d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Applied Microbiology</topic><topic>Biomedical and Life Sciences</topic><topic>Chitin</topic><topic>Chitin synthase</topic><topic>Fungicides</topic><topic>Gene expression</topic><topic>Gene silencing</topic><topic>Genetic engineering</topic><topic>Genetic transcription</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Mycology</topic><topic>Original Article</topic><topic>Pathogenicity</topic><topic>Polymerase chain reaction</topic><topic>RNA</topic><topic>RNA-mediated interference</topic><topic>Sporulation</topic><topic>Transcription</topic><topic>Virulence (Microbiology)</topic><topic>Wilt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shanmugam, Veerubommu</creatorcontrib><creatorcontrib>Sharma, Vandana</creatorcontrib><creatorcontrib>Bharti, Poonam</creatorcontrib><creatorcontrib>Jyoti, Poonam</creatorcontrib><creatorcontrib>Yadav, Sudesh Kumar</creatorcontrib><creatorcontrib>Aggarwal, Rashmi</creatorcontrib><creatorcontrib>Jain, Shekhar</creatorcontrib><collection>CrossRef</collection><collection>Gale In Context: Science</collection><jtitle>Annals of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Shanmugam, Veerubommu</au><au>Sharma, Vandana</au><au>Bharti, Poonam</au><au>Jyoti, Poonam</au><au>Yadav, Sudesh Kumar</au><au>Aggarwal, Rashmi</au><au>Jain, Shekhar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNAi induced silencing of pathogenicity genes of Fusarium spp. for vascular wilt management in tomato</atitle><jtitle>Annals of microbiology</jtitle><stitle>Ann Microbiol</stitle><date>2017-05-01</date><risdate>2017</risdate><volume>67</volume><issue>5</issue><spage>359</spage><epage>369</epage><pages>359-369</pages><issn>1590-4261</issn><eissn>1869-2044</eissn><abstract>The necessity to develop new strategies for the control of
Fusarium
wilt of tomato signifies the identification of pathogencity genes and ascertaining their role to use them as molecular tools for fungicide development or to develop transgenics. Semi-quantitative gene expression studies have identified two pathogenicity genes,
FOW2
and
chsV
, reported as ZnII)2Cys6-type transcription regulator and class V chitin synthase, respectively, as potential ones for being secreted all the time. The roles of these genes in the pathogenicity of
Fusarium oxysporum
and
F. solani
have been established by RNA interference (RNAi)-induced silencing (knockdown). The silencing vector encoding hairpin RNA of each of the gene fragment was constructed in a two-step PCR-based cloning, and introduced into the fungal genomic DNA. Silencing of either of the genes resulted in less virulent fungal phenotypes with altered physiological characteristics like sporulation and growth on solid media and a reduction in mRNA expression. The results therefore demonstrate the applicability of these pathogenicity genes as useful molecular targets for exploitation in
Fusarium
wilt control in tomato.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s13213-017-1265-3</doi><tpages>11</tpages></addata></record> |
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| subjects | Applied Microbiology Biomedical and Life Sciences Chitin Chitin synthase Fungicides Gene expression Gene silencing Genetic engineering Genetic transcription Life Sciences Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Mycology Original Article Pathogenicity Polymerase chain reaction RNA RNA-mediated interference Sporulation Transcription Virulence (Microbiology) Wilt |
| title | RNAi induced silencing of pathogenicity genes of Fusarium spp. for vascular wilt management in tomato |
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