Effects of running wheel activity and dietary HMB and B—alanine co-supplementation on muscle quality in aged male rats
Objective Loss of skeletal muscle function is linked to increased risk for loss of health and independence in older adults. Dietary interventions that can enhance aging muscle function, alone or in combination with exercise, may offer an effective way to reduce these risks. The goal of this study wa...
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| Veröffentlicht in: | The Journal of nutrition, health & aging health & aging, 2017-05, Vol.21 (5), p.554-561 |
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| creator | Russ, David W. Acksel, C. McCorkle, K. W. Edens, N. K. Garvey, S. M. |
| description | Objective
Loss of skeletal muscle function is linked to increased risk for loss of health and independence in older adults. Dietary interventions that can enhance aging muscle function, alone or in combination with exercise, may offer an effective way to reduce these risks. The goal of this study was to evaluate the muscular effects of beta-hydroxy-beta-methylbutyrate (HMB) and beta-alanine (β-Ala) co-supplementation in aged Sprague-Dawley rats with voluntary access to running wheels (RW).
Methods
Aged (20 months) rats were housed with ad libitum access to RW while on a purified diet for 4 weeks, then balanced for RW activity and assigned to either a control or an experimental diet (control + HMB and β-Ala) for the next 4 weeks (n = 10/group). At the end of the study, we assessed muscle size, in situ force and fatigability in the medial gastrocnemius muscles, as well as an array of protein markers related to various age- and activity-responsive signaling pathways.
Results
Dietary HMB+β-Ala did not improve muscle force or fatigue resistance, but a trend for increased muscle cross-sectional area (CSA) was observed (P = 0.077). As a result, rats on the experimental diet exhibited reduced muscle quality (force/CSA; P = 0.032). Dietary HMB+β-Ala reduced both the abundance of PGC1-α (P = 0.050) and the ratio of the lipidated to non-lipidated forms of microtubule-associated protein 1 light chain 3 beta (P = 0.004), markers of mitochondrial biogenesis and autophagy, respectively. Some alterations in myostatin signaling also occurred in the dietary HMB+β-Ala group. There was an unexpected difference (P = 0.046) in RW activity, which increased throughout the study in the animals on the control diet, but not in animals on the experimental diet.
Conclusions
These data suggest that the short-term addition of dietary HMB+β-Ala to modest physical activity provided little enhancement of muscle function in this model of uncomplicated aging. |
| doi_str_mv | 10.1007/s12603-016-0810-2 |
| format | Article |
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Loss of skeletal muscle function is linked to increased risk for loss of health and independence in older adults. Dietary interventions that can enhance aging muscle function, alone or in combination with exercise, may offer an effective way to reduce these risks. The goal of this study was to evaluate the muscular effects of beta-hydroxy-beta-methylbutyrate (HMB) and beta-alanine (β-Ala) co-supplementation in aged Sprague-Dawley rats with voluntary access to running wheels (RW).
Methods
Aged (20 months) rats were housed with ad libitum access to RW while on a purified diet for 4 weeks, then balanced for RW activity and assigned to either a control or an experimental diet (control + HMB and β-Ala) for the next 4 weeks (n = 10/group). At the end of the study, we assessed muscle size, in situ force and fatigability in the medial gastrocnemius muscles, as well as an array of protein markers related to various age- and activity-responsive signaling pathways.
Results
Dietary HMB+β-Ala did not improve muscle force or fatigue resistance, but a trend for increased muscle cross-sectional area (CSA) was observed (P = 0.077). As a result, rats on the experimental diet exhibited reduced muscle quality (force/CSA; P = 0.032). Dietary HMB+β-Ala reduced both the abundance of PGC1-α (P = 0.050) and the ratio of the lipidated to non-lipidated forms of microtubule-associated protein 1 light chain 3 beta (P = 0.004), markers of mitochondrial biogenesis and autophagy, respectively. Some alterations in myostatin signaling also occurred in the dietary HMB+β-Ala group. There was an unexpected difference (P = 0.046) in RW activity, which increased throughout the study in the animals on the control diet, but not in animals on the experimental diet.
Conclusions
These data suggest that the short-term addition of dietary HMB+β-Ala to modest physical activity provided little enhancement of muscle function in this model of uncomplicated aging.</description><identifier>ISSN: 1279-7707</identifier><identifier>EISSN: 1760-4788</identifier><identifier>DOI: 10.1007/s12603-016-0810-2</identifier><language>eng</language><publisher>Paris: Springer Paris</publisher><subject>Aerobics ; Age ; Aging ; Autophagy ; Diet ; Dietary supplements ; Exercise ; Fatigue ; Force ; Geriatrics/Gerontology ; Intervention ; Medicine ; Medicine & Public Health ; Muscle function ; Musculoskeletal system ; Neurosciences ; Nutrition ; Nutrition research ; Older people ; Physical fitness ; Physical therapy ; Primary Care Medicine ; Proteins ; Quality of Life Research ; Rodents</subject><ispartof>The Journal of nutrition, health & aging, 2017-05, Vol.21 (5), p.554-561</ispartof><rights>Serdi and Springer-Verlag France 2017</rights><rights>The journal of nutrition, health & aging is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-edea5665e02faeff7663bf31cf24254e784afc908c611aae3f6d7bc6be0b0e463</citedby><cites>FETCH-LOGICAL-c359t-edea5665e02faeff7663bf31cf24254e784afc908c611aae3f6d7bc6be0b0e463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12603-016-0810-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12603-016-0810-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Russ, David W.</creatorcontrib><creatorcontrib>Acksel, C.</creatorcontrib><creatorcontrib>McCorkle, K. W.</creatorcontrib><creatorcontrib>Edens, N. K.</creatorcontrib><creatorcontrib>Garvey, S. M.</creatorcontrib><title>Effects of running wheel activity and dietary HMB and B—alanine co-supplementation on muscle quality in aged male rats</title><title>The Journal of nutrition, health & aging</title><addtitle>J Nutr Health Aging</addtitle><description>Objective
Loss of skeletal muscle function is linked to increased risk for loss of health and independence in older adults. Dietary interventions that can enhance aging muscle function, alone or in combination with exercise, may offer an effective way to reduce these risks. The goal of this study was to evaluate the muscular effects of beta-hydroxy-beta-methylbutyrate (HMB) and beta-alanine (β-Ala) co-supplementation in aged Sprague-Dawley rats with voluntary access to running wheels (RW).
Methods
Aged (20 months) rats were housed with ad libitum access to RW while on a purified diet for 4 weeks, then balanced for RW activity and assigned to either a control or an experimental diet (control + HMB and β-Ala) for the next 4 weeks (n = 10/group). At the end of the study, we assessed muscle size, in situ force and fatigability in the medial gastrocnemius muscles, as well as an array of protein markers related to various age- and activity-responsive signaling pathways.
Results
Dietary HMB+β-Ala did not improve muscle force or fatigue resistance, but a trend for increased muscle cross-sectional area (CSA) was observed (P = 0.077). As a result, rats on the experimental diet exhibited reduced muscle quality (force/CSA; P = 0.032). Dietary HMB+β-Ala reduced both the abundance of PGC1-α (P = 0.050) and the ratio of the lipidated to non-lipidated forms of microtubule-associated protein 1 light chain 3 beta (P = 0.004), markers of mitochondrial biogenesis and autophagy, respectively. Some alterations in myostatin signaling also occurred in the dietary HMB+β-Ala group. There was an unexpected difference (P = 0.046) in RW activity, which increased throughout the study in the animals on the control diet, but not in animals on the experimental diet.
Conclusions
These data suggest that the short-term addition of dietary HMB+β-Ala to modest physical activity provided little enhancement of muscle function in this model of uncomplicated aging.</description><subject>Aerobics</subject><subject>Age</subject><subject>Aging</subject><subject>Autophagy</subject><subject>Diet</subject><subject>Dietary supplements</subject><subject>Exercise</subject><subject>Fatigue</subject><subject>Force</subject><subject>Geriatrics/Gerontology</subject><subject>Intervention</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Muscle function</subject><subject>Musculoskeletal system</subject><subject>Neurosciences</subject><subject>Nutrition</subject><subject>Nutrition research</subject><subject>Older people</subject><subject>Physical fitness</subject><subject>Physical therapy</subject><subject>Primary Care Medicine</subject><subject>Proteins</subject><subject>Quality of Life Research</subject><subject>Rodents</subject><issn>1279-7707</issn><issn>1760-4788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kE1OwzAQhSMEEqVwAHaWWBvs_NjJklZAkYrYwNpynHFJlTip7QDdcQhOyElwCAs2SCPNePTeG_mLonNKLikh_MrRmJEEE8owySnB8UE0o5wRnPI8PwxzzAvMOeHH0YlzW0LSrMjZLHq_0RqUd6jTyA7G1GaD3l4AGiSVr19rv0fSVKiqwUu7R6uHxc978fXxKRsZ5IBUh93Q9w20YLz0dWdQqHZwqgG0G2QzhtQGyQ1UqJVhaaV3p9GRlo2Ds98-j55vb56WK7x-vLtfXq-xSrLCY6hAZoxlQGItQWvOWFLqhCodp3GWAs9TqVVBcsUolRISzSpeKlYCKQmkLJlHF1Nub7vdAM6LbTdYE04KmheUFTyjcVDRSaVs55wFLXpbt-HHghIxAhYTYBEAixGwGD3x5HFBazZg_yT_a_oGVVGArA</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Russ, David W.</creator><creator>Acksel, C.</creator><creator>McCorkle, K. W.</creator><creator>Edens, N. K.</creator><creator>Garvey, S. M.</creator><general>Springer Paris</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope></search><sort><creationdate>20170501</creationdate><title>Effects of running wheel activity and dietary HMB and B—alanine co-supplementation on muscle quality in aged male rats</title><author>Russ, David W. ; Acksel, C. ; McCorkle, K. W. ; Edens, N. K. ; Garvey, S. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-edea5665e02faeff7663bf31cf24254e784afc908c611aae3f6d7bc6be0b0e463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aerobics</topic><topic>Age</topic><topic>Aging</topic><topic>Autophagy</topic><topic>Diet</topic><topic>Dietary supplements</topic><topic>Exercise</topic><topic>Fatigue</topic><topic>Force</topic><topic>Geriatrics/Gerontology</topic><topic>Intervention</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Muscle function</topic><topic>Musculoskeletal system</topic><topic>Neurosciences</topic><topic>Nutrition</topic><topic>Nutrition research</topic><topic>Older people</topic><topic>Physical fitness</topic><topic>Physical therapy</topic><topic>Primary Care Medicine</topic><topic>Proteins</topic><topic>Quality of Life Research</topic><topic>Rodents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Russ, David W.</creatorcontrib><creatorcontrib>Acksel, C.</creatorcontrib><creatorcontrib>McCorkle, K. W.</creatorcontrib><creatorcontrib>Edens, N. K.</creatorcontrib><creatorcontrib>Garvey, S. M.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><jtitle>The Journal of nutrition, health & aging</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Russ, David W.</au><au>Acksel, C.</au><au>McCorkle, K. W.</au><au>Edens, N. K.</au><au>Garvey, S. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of running wheel activity and dietary HMB and B—alanine co-supplementation on muscle quality in aged male rats</atitle><jtitle>The Journal of nutrition, health & aging</jtitle><stitle>J Nutr Health Aging</stitle><date>2017-05-01</date><risdate>2017</risdate><volume>21</volume><issue>5</issue><spage>554</spage><epage>561</epage><pages>554-561</pages><issn>1279-7707</issn><eissn>1760-4788</eissn><abstract>Objective
Loss of skeletal muscle function is linked to increased risk for loss of health and independence in older adults. Dietary interventions that can enhance aging muscle function, alone or in combination with exercise, may offer an effective way to reduce these risks. The goal of this study was to evaluate the muscular effects of beta-hydroxy-beta-methylbutyrate (HMB) and beta-alanine (β-Ala) co-supplementation in aged Sprague-Dawley rats with voluntary access to running wheels (RW).
Methods
Aged (20 months) rats were housed with ad libitum access to RW while on a purified diet for 4 weeks, then balanced for RW activity and assigned to either a control or an experimental diet (control + HMB and β-Ala) for the next 4 weeks (n = 10/group). At the end of the study, we assessed muscle size, in situ force and fatigability in the medial gastrocnemius muscles, as well as an array of protein markers related to various age- and activity-responsive signaling pathways.
Results
Dietary HMB+β-Ala did not improve muscle force or fatigue resistance, but a trend for increased muscle cross-sectional area (CSA) was observed (P = 0.077). As a result, rats on the experimental diet exhibited reduced muscle quality (force/CSA; P = 0.032). Dietary HMB+β-Ala reduced both the abundance of PGC1-α (P = 0.050) and the ratio of the lipidated to non-lipidated forms of microtubule-associated protein 1 light chain 3 beta (P = 0.004), markers of mitochondrial biogenesis and autophagy, respectively. Some alterations in myostatin signaling also occurred in the dietary HMB+β-Ala group. There was an unexpected difference (P = 0.046) in RW activity, which increased throughout the study in the animals on the control diet, but not in animals on the experimental diet.
Conclusions
These data suggest that the short-term addition of dietary HMB+β-Ala to modest physical activity provided little enhancement of muscle function in this model of uncomplicated aging.</abstract><cop>Paris</cop><pub>Springer Paris</pub><doi>10.1007/s12603-016-0810-2</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aerobics Age Aging Autophagy Diet Dietary supplements Exercise Fatigue Force Geriatrics/Gerontology Intervention Medicine Medicine & Public Health Muscle function Musculoskeletal system Neurosciences Nutrition Nutrition research Older people Physical fitness Physical therapy Primary Care Medicine Proteins Quality of Life Research Rodents |
| title | Effects of running wheel activity and dietary HMB and B—alanine co-supplementation on muscle quality in aged male rats |
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