Thiol antioxidants increase the intracellular level of reactive oxygen species and prolifetaion of SP2/0 mouse myeloma cells in serum-free medium

The connections between the presence of low molecular weight RSH-antioxidants (N-acetylcysteine, glutathione) in serum-free medium, generation of reactive oxygen species (ROS), and proliferation of SP2/0-SF mouse myeloma cells have been demonstrated. It is shown that the presence of RSH compounds in...

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Veröffentlicht in:	Cell and tissue biology 2017-03, Vol.11 (2), p.155-160
Hauptverfasser:	Tabakov, V. Yu, Veiko, N. N., Chestkov, V. V., Kostyuk, S. V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcysteine Antioxidants Biomedical and Life Sciences Cell Biology Cell growth Cell proliferation Glutathione Life Sciences Molecular weight Multiple myeloma Myeloma Reactive oxygen species Rodents Serum-free medium
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container_title	Cell and tissue biology
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creator	Tabakov, V. Yu Veiko, N. N. Chestkov, V. V. Kostyuk, S. V.
description	The connections between the presence of low molecular weight RSH-antioxidants (N-acetylcysteine, glutathione) in serum-free medium, generation of reactive oxygen species (ROS), and proliferation of SP2/0-SF mouse myeloma cells have been demonstrated. It is shown that the presence of RSH compounds in the medium within the studied range of concentrations changed the contents of ROS in cells and had a dose-dependent effect on cell proliferation. Stimulation of the proliferative activity did not depend on the nature of an RSH compound. The optimal concentration for the both antioxidants was 0.2 mM. A further increase of the concentration led to inhibition of cell proliferation to different degrees for N-acetylcysteine and glutathione.
doi_str_mv	10.1134/S1990519X17020067
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Biol</stitle><date>2017-03-01</date><risdate>2017</risdate><volume>11</volume><issue>2</issue><spage>155</spage><epage>160</epage><pages>155-160</pages><issn>1990-519X</issn><eissn>1990-5203</eissn><abstract>The connections between the presence of low molecular weight RSH-antioxidants (N-acetylcysteine, glutathione) in serum-free medium, generation of reactive oxygen species (ROS), and proliferation of SP2/0-SF mouse myeloma cells have been demonstrated. It is shown that the presence of RSH compounds in the medium within the studied range of concentrations changed the contents of ROS in cells and had a dose-dependent effect on cell proliferation. Stimulation of the proliferative activity did not depend on the nature of an RSH compound. The optimal concentration for the both antioxidants was 0.2 mM. 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subjects	Acetylcysteine Antioxidants Biomedical and Life Sciences Cell Biology Cell growth Cell proliferation Glutathione Life Sciences Molecular weight Multiple myeloma Myeloma Reactive oxygen species Rodents Serum-free medium
title	Thiol antioxidants increase the intracellular level of reactive oxygen species and prolifetaion of SP2/0 mouse myeloma cells in serum-free medium
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