Structure of the N-acetylpseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii NIPH67
Capsular polysaccharide (CPS) was isolated from a nosocomial pathogen Acinetobacter baumannii ( A. baumannii ) NIPH67 and studied by sugar analysis, Smith degradation, and 1 H and 13 C NMR spectroscopy. The CPS was found to contain 5,7-diacetamido-3,5,7,9-tetradeoxy- l - glycero - l - manno -non-2-u...
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| Veröffentlicht in: | Russian chemical bulletin 2016-02, Vol.65 (2), p.588-591 |
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| creator | Arbatsky, N. P. Shneider, M. M. Shashkov, A. S. Popova, A. V. Miroshnikov, K. A. Volozhantsev, N. V. Knirel, Y. A. |
| description | Capsular polysaccharide (CPS) was isolated from a nosocomial pathogen
Acinetobacter baumannii
(
A. baumannii
) NIPH67 and studied by sugar analysis, Smith degradation, and
1
H and
13
C NMR spectroscopy. The CPS was found to contain 5,7-diacetamido-3,5,7,9-tetradeoxy-
l
-
glycero
-
l
-
manno
-non-2-ulosonic acid (di-
N
-acetylpseudaminic acid, Pse5Ac7Ac), and the structure of the linear trisaccharide repeating unit of the CPS was established as →4)-α-Pse
p
5Ac7Ac-(2→6)-β-
d
-Gal
p
-(1→3)-α-
d
-Gal
p
NAc-(1→. The genetic content of the capsule biosynthesis cluster of
A. baumannii
NIPH67, designated KL33, is consistent with the established CPS structure, and thus the capsule of the investigated strain was assigned to K33 group. Functions of proteins including two glycosyltransferases encoded by the genes of the K33 locus were assigned based on the structure of CPS and by the comparison with related proteins of other capsular types of
A. baumannii
. |
| doi_str_mv | 10.1007/s11172-016-1342-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1880843535</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1880843535</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-9507478a899a781167bbfa3ecb73474a37a916e30bca3298968b6ddd4f767583</originalsourceid><addsrcrecordid>eNp1kM9LwzAUx4MoOKd_gLeC52he0ybpcQx1wpiCu4fXNN06urQm6aH_vZ3z4EXe4b0H3x_wIeQe2CMwJp8CAMiUMhAUeJbS8YLMIJecFiDhcrqZEDRPVX5NbkI4MMZSpdSMuM_oBxMHb5OuTuLeJhuKxsax7YMdKjw2rjEJmqaipnMRp9ftEoN9GFr0Sd-1Y0Bj9uib6idiYRpnY1eiidYnJQ5HdK5pks3bx0rIW3JVYxvs3e-ek-3L83a5ouv317flYk0NBxFpkTOZSYWqKFAqACHLskZuTSl5JjPkEgsQlrPSIE8LVQhViqqqsloKmSs-Jw_n2N53X4MNUR-6wbupUYNSTGU8n2ZO4KwyvgvB21r3vjmiHzUwfaKqz1T1RFWfqOpx8qRnT5i0bmf9n-R_Td8f43uA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1880843535</pqid></control><display><type>article</type><title>Structure of the N-acetylpseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii NIPH67</title><source>SpringerLink Journals - AutoHoldings</source><creator>Arbatsky, N. P. ; Shneider, M. M. ; Shashkov, A. S. ; Popova, A. V. ; Miroshnikov, K. A. ; Volozhantsev, N. V. ; Knirel, Y. A.</creator><creatorcontrib>Arbatsky, N. P. ; Shneider, M. M. ; Shashkov, A. S. ; Popova, A. V. ; Miroshnikov, K. A. ; Volozhantsev, N. V. ; Knirel, Y. A.</creatorcontrib><description>Capsular polysaccharide (CPS) was isolated from a nosocomial pathogen
Acinetobacter baumannii
(
A. baumannii
) NIPH67 and studied by sugar analysis, Smith degradation, and
1
H and
13
C NMR spectroscopy. The CPS was found to contain 5,7-diacetamido-3,5,7,9-tetradeoxy-
l
-
glycero
-
l
-
manno
-non-2-ulosonic acid (di-
N
-acetylpseudaminic acid, Pse5Ac7Ac), and the structure of the linear trisaccharide repeating unit of the CPS was established as →4)-α-Pse
p
5Ac7Ac-(2→6)-β-
d
-Gal
p
-(1→3)-α-
d
-Gal
p
NAc-(1→. The genetic content of the capsule biosynthesis cluster of
A. baumannii
NIPH67, designated KL33, is consistent with the established CPS structure, and thus the capsule of the investigated strain was assigned to K33 group. Functions of proteins including two glycosyltransferases encoded by the genes of the K33 locus were assigned based on the structure of CPS and by the comparison with related proteins of other capsular types of
A. baumannii
.</description><identifier>ISSN: 1066-5285</identifier><identifier>EISSN: 1573-9171</identifier><identifier>DOI: 10.1007/s11172-016-1342-y</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biosynthesis ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Full Articles ; Inorganic Chemistry ; NMR spectroscopy ; Oligosaccharides ; Organic Chemistry ; Polysaccharides ; Proteins ; Strain analysis</subject><ispartof>Russian chemical bulletin, 2016-02, Vol.65 (2), p.588-591</ispartof><rights>Springer Science+Business Media New York 2016</rights><rights>Copyright Springer Science & Business Media 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-9507478a899a781167bbfa3ecb73474a37a916e30bca3298968b6ddd4f767583</citedby><cites>FETCH-LOGICAL-c316t-9507478a899a781167bbfa3ecb73474a37a916e30bca3298968b6ddd4f767583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11172-016-1342-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11172-016-1342-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Arbatsky, N. P.</creatorcontrib><creatorcontrib>Shneider, M. M.</creatorcontrib><creatorcontrib>Shashkov, A. S.</creatorcontrib><creatorcontrib>Popova, A. V.</creatorcontrib><creatorcontrib>Miroshnikov, K. A.</creatorcontrib><creatorcontrib>Volozhantsev, N. V.</creatorcontrib><creatorcontrib>Knirel, Y. A.</creatorcontrib><title>Structure of the N-acetylpseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii NIPH67</title><title>Russian chemical bulletin</title><addtitle>Russ Chem Bull</addtitle><description>Capsular polysaccharide (CPS) was isolated from a nosocomial pathogen
Acinetobacter baumannii
(
A. baumannii
) NIPH67 and studied by sugar analysis, Smith degradation, and
1
H and
13
C NMR spectroscopy. The CPS was found to contain 5,7-diacetamido-3,5,7,9-tetradeoxy-
l
-
glycero
-
l
-
manno
-non-2-ulosonic acid (di-
N
-acetylpseudaminic acid, Pse5Ac7Ac), and the structure of the linear trisaccharide repeating unit of the CPS was established as →4)-α-Pse
p
5Ac7Ac-(2→6)-β-
d
-Gal
p
-(1→3)-α-
d
-Gal
p
NAc-(1→. The genetic content of the capsule biosynthesis cluster of
A. baumannii
NIPH67, designated KL33, is consistent with the established CPS structure, and thus the capsule of the investigated strain was assigned to K33 group. Functions of proteins including two glycosyltransferases encoded by the genes of the K33 locus were assigned based on the structure of CPS and by the comparison with related proteins of other capsular types of
A. baumannii
.</description><subject>Biosynthesis</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Full Articles</subject><subject>Inorganic Chemistry</subject><subject>NMR spectroscopy</subject><subject>Oligosaccharides</subject><subject>Organic Chemistry</subject><subject>Polysaccharides</subject><subject>Proteins</subject><subject>Strain analysis</subject><issn>1066-5285</issn><issn>1573-9171</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kM9LwzAUx4MoOKd_gLeC52he0ybpcQx1wpiCu4fXNN06urQm6aH_vZ3z4EXe4b0H3x_wIeQe2CMwJp8CAMiUMhAUeJbS8YLMIJecFiDhcrqZEDRPVX5NbkI4MMZSpdSMuM_oBxMHb5OuTuLeJhuKxsax7YMdKjw2rjEJmqaipnMRp9ftEoN9GFr0Sd-1Y0Bj9uib6idiYRpnY1eiidYnJQ5HdK5pks3bx0rIW3JVYxvs3e-ek-3L83a5ouv317flYk0NBxFpkTOZSYWqKFAqACHLskZuTSl5JjPkEgsQlrPSIE8LVQhViqqqsloKmSs-Jw_n2N53X4MNUR-6wbupUYNSTGU8n2ZO4KwyvgvB21r3vjmiHzUwfaKqz1T1RFWfqOpx8qRnT5i0bmf9n-R_Td8f43uA</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Arbatsky, N. P.</creator><creator>Shneider, M. M.</creator><creator>Shashkov, A. S.</creator><creator>Popova, A. V.</creator><creator>Miroshnikov, K. A.</creator><creator>Volozhantsev, N. V.</creator><creator>Knirel, Y. A.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20160201</creationdate><title>Structure of the N-acetylpseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii NIPH67</title><author>Arbatsky, N. P. ; Shneider, M. M. ; Shashkov, A. S. ; Popova, A. V. ; Miroshnikov, K. A. ; Volozhantsev, N. V. ; Knirel, Y. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-9507478a899a781167bbfa3ecb73474a37a916e30bca3298968b6ddd4f767583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Biosynthesis</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Full Articles</topic><topic>Inorganic Chemistry</topic><topic>NMR spectroscopy</topic><topic>Oligosaccharides</topic><topic>Organic Chemistry</topic><topic>Polysaccharides</topic><topic>Proteins</topic><topic>Strain analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arbatsky, N. P.</creatorcontrib><creatorcontrib>Shneider, M. M.</creatorcontrib><creatorcontrib>Shashkov, A. S.</creatorcontrib><creatorcontrib>Popova, A. V.</creatorcontrib><creatorcontrib>Miroshnikov, K. A.</creatorcontrib><creatorcontrib>Volozhantsev, N. V.</creatorcontrib><creatorcontrib>Knirel, Y. A.</creatorcontrib><collection>CrossRef</collection><jtitle>Russian chemical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arbatsky, N. P.</au><au>Shneider, M. M.</au><au>Shashkov, A. S.</au><au>Popova, A. V.</au><au>Miroshnikov, K. A.</au><au>Volozhantsev, N. V.</au><au>Knirel, Y. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure of the N-acetylpseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii NIPH67</atitle><jtitle>Russian chemical bulletin</jtitle><stitle>Russ Chem Bull</stitle><date>2016-02-01</date><risdate>2016</risdate><volume>65</volume><issue>2</issue><spage>588</spage><epage>591</epage><pages>588-591</pages><issn>1066-5285</issn><eissn>1573-9171</eissn><abstract>Capsular polysaccharide (CPS) was isolated from a nosocomial pathogen
Acinetobacter baumannii
(
A. baumannii
) NIPH67 and studied by sugar analysis, Smith degradation, and
1
H and
13
C NMR spectroscopy. The CPS was found to contain 5,7-diacetamido-3,5,7,9-tetradeoxy-
l
-
glycero
-
l
-
manno
-non-2-ulosonic acid (di-
N
-acetylpseudaminic acid, Pse5Ac7Ac), and the structure of the linear trisaccharide repeating unit of the CPS was established as →4)-α-Pse
p
5Ac7Ac-(2→6)-β-
d
-Gal
p
-(1→3)-α-
d
-Gal
p
NAc-(1→. The genetic content of the capsule biosynthesis cluster of
A. baumannii
NIPH67, designated KL33, is consistent with the established CPS structure, and thus the capsule of the investigated strain was assigned to K33 group. Functions of proteins including two glycosyltransferases encoded by the genes of the K33 locus were assigned based on the structure of CPS and by the comparison with related proteins of other capsular types of
A. baumannii
.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11172-016-1342-y</doi><tpages>4</tpages></addata></record> |
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| subjects | Biosynthesis Chemistry Chemistry and Materials Science Chemistry/Food Science Full Articles Inorganic Chemistry NMR spectroscopy Oligosaccharides Organic Chemistry Polysaccharides Proteins Strain analysis |
| title | Structure of the N-acetylpseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii NIPH67 |
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