An atomic-force-microscopy study of the structure of surface layers of intact fibroblasts
Intact embryonic fibroblasts on a collagen-treated substrate have been studied by atomic-force microscopy (AFM) using probes of two types: (i) standard probes with tip curvature radii of 2–10 nm and (ii) special probes with a calibrated 325-nm SiO 2 ball radius at the tip apex. It is established tha...
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| Veröffentlicht in: | Technical physics letters 2017-02, Vol.43 (2), p.209-212 |
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| creator | Khalisov, M. M. Ankudinov, A. V. Penniyaynen, V. A. Nyapshaev, I. A. Kipenko, A. V. Timoshchuk, K. I. Podzorova, S. A. Krylov, B. V. |
| description | Intact embryonic fibroblasts on a collagen-treated substrate have been studied by atomic-force microscopy (AFM) using probes of two types: (i) standard probes with tip curvature radii of 2–10 nm and (ii) special probes with a calibrated 325-nm SiO
2
ball radius at the tip apex. It is established that, irrespective of probe type, the average maximum fibroblast height is on a level of ~1.7 μm and the average stiffness of the probe–cell contact amounts to ~16.5 mN/m. The obtained AFM data reveal a peculiarity of the fibroblast structure, whereby its external layers move as a rigid shell relative to the interior and can be pressed inside to a depth dependent on the load only. |
| doi_str_mv | 10.1134/S1063785017020195 |
| format | Article |
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2
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2
ball radius at the tip apex. It is established that, irrespective of probe type, the average maximum fibroblast height is on a level of ~1.7 μm and the average stiffness of the probe–cell contact amounts to ~16.5 mN/m. The obtained AFM data reveal a peculiarity of the fibroblast structure, whereby its external layers move as a rigid shell relative to the interior and can be pressed inside to a depth dependent on the load only.</description><subject>Atomic force microscopy</subject><subject>Classical and Continuum Physics</subject><subject>Fibroblasts</subject><subject>Microscopes</subject><subject>Microscopy</subject><subject>Physics</subject><subject>Physics and Astronomy</subject><subject>Silicon dioxide</subject><subject>Stiffness</subject><subject>Substrates</subject><subject>Surface layers</subject><issn>1063-7850</issn><issn>1090-6533</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp1UD1PwzAQtRBIlMIPYIvEbLiLHdsZqwooUiUGYGCKHNeGVG1cbGfIvydWGZAQ0929e-_dByHXCLeIjN-9IAgmVQUooQSsqxMyQ6iBioqx05wLRnP_nFzEuAUAVVb1jLwv-kInv-8MdT4YS6cs-Gj8YSxiGjZj4V2RPu1UhMGkIdgMxCE4bWyx06MNMSNdn7RJheva4NudjilekjOnd9Fe_cQ5eXu4f12u6Pr58Wm5WFPDUCRqhdK1dhtVTiturBbGcQUtq5xEVkJrpEYuuVQtr7UxWCkQijMlGHeG14LNyc3R9xD812BjarZ-CP00skGlQHJeiszCIytfF4N1zSF0ex3GBqHJH2z-fHDSlEdNnLj9hw2_nP8VfQOb_3JP</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Khalisov, M. M.</creator><creator>Ankudinov, A. V.</creator><creator>Penniyaynen, V. A.</creator><creator>Nyapshaev, I. A.</creator><creator>Kipenko, A. V.</creator><creator>Timoshchuk, K. I.</creator><creator>Podzorova, S. A.</creator><creator>Krylov, B. V.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20170201</creationdate><title>An atomic-force-microscopy study of the structure of surface layers of intact fibroblasts</title><author>Khalisov, M. M. ; Ankudinov, A. V. ; Penniyaynen, V. A. ; Nyapshaev, I. A. ; Kipenko, A. V. ; Timoshchuk, K. I. ; Podzorova, S. A. ; Krylov, B. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-e68a9afd82106dea6cf480b35f71320bc7a147478b49acc158068438634fc4963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Atomic force microscopy</topic><topic>Classical and Continuum Physics</topic><topic>Fibroblasts</topic><topic>Microscopes</topic><topic>Microscopy</topic><topic>Physics</topic><topic>Physics and Astronomy</topic><topic>Silicon dioxide</topic><topic>Stiffness</topic><topic>Substrates</topic><topic>Surface layers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khalisov, M. M.</creatorcontrib><creatorcontrib>Ankudinov, A. V.</creatorcontrib><creatorcontrib>Penniyaynen, V. A.</creatorcontrib><creatorcontrib>Nyapshaev, I. A.</creatorcontrib><creatorcontrib>Kipenko, A. V.</creatorcontrib><creatorcontrib>Timoshchuk, K. I.</creatorcontrib><creatorcontrib>Podzorova, S. A.</creatorcontrib><creatorcontrib>Krylov, B. V.</creatorcontrib><collection>CrossRef</collection><jtitle>Technical physics letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khalisov, M. M.</au><au>Ankudinov, A. V.</au><au>Penniyaynen, V. A.</au><au>Nyapshaev, I. A.</au><au>Kipenko, A. V.</au><au>Timoshchuk, K. I.</au><au>Podzorova, S. A.</au><au>Krylov, B. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An atomic-force-microscopy study of the structure of surface layers of intact fibroblasts</atitle><jtitle>Technical physics letters</jtitle><stitle>Tech. Phys. Lett</stitle><date>2017-02-01</date><risdate>2017</risdate><volume>43</volume><issue>2</issue><spage>209</spage><epage>212</epage><pages>209-212</pages><issn>1063-7850</issn><eissn>1090-6533</eissn><abstract>Intact embryonic fibroblasts on a collagen-treated substrate have been studied by atomic-force microscopy (AFM) using probes of two types: (i) standard probes with tip curvature radii of 2–10 nm and (ii) special probes with a calibrated 325-nm SiO
2
ball radius at the tip apex. It is established that, irrespective of probe type, the average maximum fibroblast height is on a level of ~1.7 μm and the average stiffness of the probe–cell contact amounts to ~16.5 mN/m. The obtained AFM data reveal a peculiarity of the fibroblast structure, whereby its external layers move as a rigid shell relative to the interior and can be pressed inside to a depth dependent on the load only.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1063785017020195</doi><tpages>4</tpages></addata></record> |
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| subjects | Atomic force microscopy Classical and Continuum Physics Fibroblasts Microscopes Microscopy Physics Physics and Astronomy Silicon dioxide Stiffness Substrates Surface layers |
| title | An atomic-force-microscopy study of the structure of surface layers of intact fibroblasts |
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