ctDNA interaction of Co-containing Keggin polyoxomolybdate and in vitro antitumor activity of free and its nano-encapsulated derivatives
Polyoxometalates (POMs) are negatively charged clusters consisting of transition metals and oxygen atoms. The antiviral and antitumor activities are the dominant activities of POMs in pharmacology and medicine. Based on Co-containing Keggin polyoxomolybdate (K 6 [SiMo 11 O 39 Co(H 2 O)]. n H 2 O), n...
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| Veröffentlicht in: | Journal of the Iranian Chemical Society 2016-10, Vol.13 (10), p.1895-1904 |
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| creator | Dianat, S. Bordbar, A. K. Tangestaninejad, S. Zarkesh-Esfahani, S. H. Habibi, P. Abbasi Kajani, A. |
| description | Polyoxometalates (POMs) are negatively charged clusters consisting of transition metals and oxygen atoms. The antiviral and antitumor activities are the dominant activities of POMs in pharmacology and medicine. Based on Co-containing Keggin polyoxomolybdate (K
6
[SiMo
11
O
39
Co(H
2
O)].
n
H
2
O), nanosized starch, and lipid-encapsulated derivatives (abbreviated as SiMo
11
Co, SEP and LEP, respectively) were synthesized and characterized by FT-IR spectroscopy, ICP, TG analysis, SEM and TEM images. The results show that the SiMo
11
Co retains its parent structure after encapsulation by starch and lipid nanoparticles. The biological activity of SiMo
11
Co has been evaluated by investigating its binding ability to calf thymus DNA (ctDNA), using UV–Vis absorption spectroscopy, fluorescence quenching and fluorescence Scatchard plots. The obtained results of absorption titration rule out the intercalating binding mode and propose the groove or outside stacking binding for SiMo
11
Co. These results were authenticated by fluorescence quenching experiments and scatchard plots. Absorption spectral traces reveal 10.21 % hyperchromism for SiMo
11
Co. The value of 7.6 × 10
3
M
−1
was obtained for binding constant (K
b
) of SiMo
11
Co to ctDNA. Furthermore, the in vitro antitumor activity of SiMo
11
Co and nano-encapsulated forms was investigated using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay that was carried out on two types of human cancer cells, MCF-7 (breast cancer cells) and HEK-293 (Human Embryonic Kidney). The results represent the enhancement of cell penetration and antitumor activity of SiMo
11
Co due to its encapsulation in starch or lipid nanoparticles. However, this observed enhancement for the lipid relative to the starch nanocapsule can be attributed to its smaller size. |
| doi_str_mv | 10.1007/s13738-016-0906-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1880740971</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1880740971</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-3fba3ba541d3943f0362281db7041caaf132bea9004b42312d20144e5e44934e3</originalsourceid><addsrcrecordid>eNp1kMtKxDAUhosoOI4-gLuA6-jJpbflMF5x0I2Cu5C2ackwk4xJOtg38LFN6QhuXJ1zwvd_gT9JLglcE4D8xhOWswIDyTCUkOHhKJmRnKWYcloc_-6Qf5wmZ96vAdIcUj5Lvutw-7JA2gTlZB20Nci2aGlxbU2Q2mjToWfVddqgnd0M9stu46gaGRSSpolBtNfB2XgEHfqtdWjUxLdhFLVOHbjgkZHGYmVqufP9Jgoa1Cin9zLiyp8nJ63ceHVxmPPk_f7ubfmIV68PT8vFCteMZAGztpKskiknDSs5a4FllBakqXLgpJayJYxWSpYAvOKUEdpQIJyrVHFeMq7YPLmavDtnP3vlg1jb3pn4pSBFATmHMieRIhNVO-u9U63YOb2VbhAExFi4mAoXsXAxFi6GmKFTxkfWdMr9Mf8b-gGTlYYj</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1880740971</pqid></control><display><type>article</type><title>ctDNA interaction of Co-containing Keggin polyoxomolybdate and in vitro antitumor activity of free and its nano-encapsulated derivatives</title><source>SpringerLink Journals - AutoHoldings</source><creator>Dianat, S. ; Bordbar, A. K. ; Tangestaninejad, S. ; Zarkesh-Esfahani, S. H. ; Habibi, P. ; Abbasi Kajani, A.</creator><creatorcontrib>Dianat, S. ; Bordbar, A. K. ; Tangestaninejad, S. ; Zarkesh-Esfahani, S. H. ; Habibi, P. ; Abbasi Kajani, A.</creatorcontrib><description>Polyoxometalates (POMs) are negatively charged clusters consisting of transition metals and oxygen atoms. The antiviral and antitumor activities are the dominant activities of POMs in pharmacology and medicine. Based on Co-containing Keggin polyoxomolybdate (K
6
[SiMo
11
O
39
Co(H
2
O)].
n
H
2
O), nanosized starch, and lipid-encapsulated derivatives (abbreviated as SiMo
11
Co, SEP and LEP, respectively) were synthesized and characterized by FT-IR spectroscopy, ICP, TG analysis, SEM and TEM images. The results show that the SiMo
11
Co retains its parent structure after encapsulation by starch and lipid nanoparticles. The biological activity of SiMo
11
Co has been evaluated by investigating its binding ability to calf thymus DNA (ctDNA), using UV–Vis absorption spectroscopy, fluorescence quenching and fluorescence Scatchard plots. The obtained results of absorption titration rule out the intercalating binding mode and propose the groove or outside stacking binding for SiMo
11
Co. These results were authenticated by fluorescence quenching experiments and scatchard plots. Absorption spectral traces reveal 10.21 % hyperchromism for SiMo
11
Co. The value of 7.6 × 10
3
M
−1
was obtained for binding constant (K
b
) of SiMo
11
Co to ctDNA. Furthermore, the in vitro antitumor activity of SiMo
11
Co and nano-encapsulated forms was investigated using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay that was carried out on two types of human cancer cells, MCF-7 (breast cancer cells) and HEK-293 (Human Embryonic Kidney). The results represent the enhancement of cell penetration and antitumor activity of SiMo
11
Co due to its encapsulation in starch or lipid nanoparticles. However, this observed enhancement for the lipid relative to the starch nanocapsule can be attributed to its smaller size.</description><identifier>ISSN: 1735-207X</identifier><identifier>EISSN: 1735-2428</identifier><identifier>DOI: 10.1007/s13738-016-0906-y</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Absorption spectroscopy ; Analytical Chemistry ; Anticancer properties ; Binding ; Biochemistry ; Biological activity ; Biological effects ; Chemistry ; Chemistry and Materials Science ; Encapsulation ; Fluorescence ; Grooves ; Infrared spectroscopy ; Inorganic Chemistry ; Lipids ; Nanoparticles ; Organic Chemistry ; Original Paper ; Oxygen atoms ; Pharmacology ; Physical Chemistry ; Polyoxometallates ; Quenching ; Scatchard curves ; Titration ; Transition metals</subject><ispartof>Journal of the Iranian Chemical Society, 2016-10, Vol.13 (10), p.1895-1904</ispartof><rights>Iranian Chemical Society 2016</rights><rights>Copyright Springer Science & Business Media 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-3fba3ba541d3943f0362281db7041caaf132bea9004b42312d20144e5e44934e3</citedby><cites>FETCH-LOGICAL-c316t-3fba3ba541d3943f0362281db7041caaf132bea9004b42312d20144e5e44934e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13738-016-0906-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13738-016-0906-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Dianat, S.</creatorcontrib><creatorcontrib>Bordbar, A. K.</creatorcontrib><creatorcontrib>Tangestaninejad, S.</creatorcontrib><creatorcontrib>Zarkesh-Esfahani, S. H.</creatorcontrib><creatorcontrib>Habibi, P.</creatorcontrib><creatorcontrib>Abbasi Kajani, A.</creatorcontrib><title>ctDNA interaction of Co-containing Keggin polyoxomolybdate and in vitro antitumor activity of free and its nano-encapsulated derivatives</title><title>Journal of the Iranian Chemical Society</title><addtitle>J IRAN CHEM SOC</addtitle><description>Polyoxometalates (POMs) are negatively charged clusters consisting of transition metals and oxygen atoms. The antiviral and antitumor activities are the dominant activities of POMs in pharmacology and medicine. Based on Co-containing Keggin polyoxomolybdate (K
6
[SiMo
11
O
39
Co(H
2
O)].
n
H
2
O), nanosized starch, and lipid-encapsulated derivatives (abbreviated as SiMo
11
Co, SEP and LEP, respectively) were synthesized and characterized by FT-IR spectroscopy, ICP, TG analysis, SEM and TEM images. The results show that the SiMo
11
Co retains its parent structure after encapsulation by starch and lipid nanoparticles. The biological activity of SiMo
11
Co has been evaluated by investigating its binding ability to calf thymus DNA (ctDNA), using UV–Vis absorption spectroscopy, fluorescence quenching and fluorescence Scatchard plots. The obtained results of absorption titration rule out the intercalating binding mode and propose the groove or outside stacking binding for SiMo
11
Co. These results were authenticated by fluorescence quenching experiments and scatchard plots. Absorption spectral traces reveal 10.21 % hyperchromism for SiMo
11
Co. The value of 7.6 × 10
3
M
−1
was obtained for binding constant (K
b
) of SiMo
11
Co to ctDNA. Furthermore, the in vitro antitumor activity of SiMo
11
Co and nano-encapsulated forms was investigated using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay that was carried out on two types of human cancer cells, MCF-7 (breast cancer cells) and HEK-293 (Human Embryonic Kidney). The results represent the enhancement of cell penetration and antitumor activity of SiMo
11
Co due to its encapsulation in starch or lipid nanoparticles. However, this observed enhancement for the lipid relative to the starch nanocapsule can be attributed to its smaller size.</description><subject>Absorption spectroscopy</subject><subject>Analytical Chemistry</subject><subject>Anticancer properties</subject><subject>Binding</subject><subject>Biochemistry</subject><subject>Biological activity</subject><subject>Biological effects</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Encapsulation</subject><subject>Fluorescence</subject><subject>Grooves</subject><subject>Infrared spectroscopy</subject><subject>Inorganic Chemistry</subject><subject>Lipids</subject><subject>Nanoparticles</subject><subject>Organic Chemistry</subject><subject>Original Paper</subject><subject>Oxygen atoms</subject><subject>Pharmacology</subject><subject>Physical Chemistry</subject><subject>Polyoxometallates</subject><subject>Quenching</subject><subject>Scatchard curves</subject><subject>Titration</subject><subject>Transition metals</subject><issn>1735-207X</issn><issn>1735-2428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kMtKxDAUhosoOI4-gLuA6-jJpbflMF5x0I2Cu5C2ackwk4xJOtg38LFN6QhuXJ1zwvd_gT9JLglcE4D8xhOWswIDyTCUkOHhKJmRnKWYcloc_-6Qf5wmZ96vAdIcUj5Lvutw-7JA2gTlZB20Nci2aGlxbU2Q2mjToWfVddqgnd0M9stu46gaGRSSpolBtNfB2XgEHfqtdWjUxLdhFLVOHbjgkZHGYmVqufP9Jgoa1Cin9zLiyp8nJ63ceHVxmPPk_f7ubfmIV68PT8vFCteMZAGztpKskiknDSs5a4FllBakqXLgpJayJYxWSpYAvOKUEdpQIJyrVHFeMq7YPLmavDtnP3vlg1jb3pn4pSBFATmHMieRIhNVO-u9U63YOb2VbhAExFi4mAoXsXAxFi6GmKFTxkfWdMr9Mf8b-gGTlYYj</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Dianat, S.</creator><creator>Bordbar, A. K.</creator><creator>Tangestaninejad, S.</creator><creator>Zarkesh-Esfahani, S. H.</creator><creator>Habibi, P.</creator><creator>Abbasi Kajani, A.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20161001</creationdate><title>ctDNA interaction of Co-containing Keggin polyoxomolybdate and in vitro antitumor activity of free and its nano-encapsulated derivatives</title><author>Dianat, S. ; Bordbar, A. K. ; Tangestaninejad, S. ; Zarkesh-Esfahani, S. H. ; Habibi, P. ; Abbasi Kajani, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-3fba3ba541d3943f0362281db7041caaf132bea9004b42312d20144e5e44934e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Absorption spectroscopy</topic><topic>Analytical Chemistry</topic><topic>Anticancer properties</topic><topic>Binding</topic><topic>Biochemistry</topic><topic>Biological activity</topic><topic>Biological effects</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Encapsulation</topic><topic>Fluorescence</topic><topic>Grooves</topic><topic>Infrared spectroscopy</topic><topic>Inorganic Chemistry</topic><topic>Lipids</topic><topic>Nanoparticles</topic><topic>Organic Chemistry</topic><topic>Original Paper</topic><topic>Oxygen atoms</topic><topic>Pharmacology</topic><topic>Physical Chemistry</topic><topic>Polyoxometallates</topic><topic>Quenching</topic><topic>Scatchard curves</topic><topic>Titration</topic><topic>Transition metals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dianat, S.</creatorcontrib><creatorcontrib>Bordbar, A. K.</creatorcontrib><creatorcontrib>Tangestaninejad, S.</creatorcontrib><creatorcontrib>Zarkesh-Esfahani, S. H.</creatorcontrib><creatorcontrib>Habibi, P.</creatorcontrib><creatorcontrib>Abbasi Kajani, A.</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of the Iranian Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dianat, S.</au><au>Bordbar, A. K.</au><au>Tangestaninejad, S.</au><au>Zarkesh-Esfahani, S. H.</au><au>Habibi, P.</au><au>Abbasi Kajani, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ctDNA interaction of Co-containing Keggin polyoxomolybdate and in vitro antitumor activity of free and its nano-encapsulated derivatives</atitle><jtitle>Journal of the Iranian Chemical Society</jtitle><stitle>J IRAN CHEM SOC</stitle><date>2016-10-01</date><risdate>2016</risdate><volume>13</volume><issue>10</issue><spage>1895</spage><epage>1904</epage><pages>1895-1904</pages><issn>1735-207X</issn><eissn>1735-2428</eissn><abstract>Polyoxometalates (POMs) are negatively charged clusters consisting of transition metals and oxygen atoms. The antiviral and antitumor activities are the dominant activities of POMs in pharmacology and medicine. Based on Co-containing Keggin polyoxomolybdate (K
6
[SiMo
11
O
39
Co(H
2
O)].
n
H
2
O), nanosized starch, and lipid-encapsulated derivatives (abbreviated as SiMo
11
Co, SEP and LEP, respectively) were synthesized and characterized by FT-IR spectroscopy, ICP, TG analysis, SEM and TEM images. The results show that the SiMo
11
Co retains its parent structure after encapsulation by starch and lipid nanoparticles. The biological activity of SiMo
11
Co has been evaluated by investigating its binding ability to calf thymus DNA (ctDNA), using UV–Vis absorption spectroscopy, fluorescence quenching and fluorescence Scatchard plots. The obtained results of absorption titration rule out the intercalating binding mode and propose the groove or outside stacking binding for SiMo
11
Co. These results were authenticated by fluorescence quenching experiments and scatchard plots. Absorption spectral traces reveal 10.21 % hyperchromism for SiMo
11
Co. The value of 7.6 × 10
3
M
−1
was obtained for binding constant (K
b
) of SiMo
11
Co to ctDNA. Furthermore, the in vitro antitumor activity of SiMo
11
Co and nano-encapsulated forms was investigated using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay that was carried out on two types of human cancer cells, MCF-7 (breast cancer cells) and HEK-293 (Human Embryonic Kidney). The results represent the enhancement of cell penetration and antitumor activity of SiMo
11
Co due to its encapsulation in starch or lipid nanoparticles. However, this observed enhancement for the lipid relative to the starch nanocapsule can be attributed to its smaller size.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s13738-016-0906-y</doi><tpages>10</tpages></addata></record> |
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| subjects | Absorption spectroscopy Analytical Chemistry Anticancer properties Binding Biochemistry Biological activity Biological effects Chemistry Chemistry and Materials Science Encapsulation Fluorescence Grooves Infrared spectroscopy Inorganic Chemistry Lipids Nanoparticles Organic Chemistry Original Paper Oxygen atoms Pharmacology Physical Chemistry Polyoxometallates Quenching Scatchard curves Titration Transition metals |
| title | ctDNA interaction of Co-containing Keggin polyoxomolybdate and in vitro antitumor activity of free and its nano-encapsulated derivatives |
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