Mediator cyclin-dependent kinases upregulate transcription of inflammatory genes in cooperation with NF-[kappa]B and C/EBP[beta] on stimulation of Toll-like receptor 9

In eukaryotes, the Mediator complex has important roles in regulation of transcription by RNA polymerase II. Mediator is a large complex with more than 20 subunits that form head, middle, tail and CDK/cyclin modules. Among them, CDK8 and/or CDK19 (CDK8/19), and their counterpart cyclin C, form the C...

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Veröffentlicht in:Genes to cells : devoted to molecular & cellular mechanisms 2017-03, Vol.22 (3), p.265
Hauptverfasser: Yamamoto, Seiji, Hagihara, Tomoko, Horiuchi, Yoshiyuki, Okui, Akira, Wani, Shotaro, Yoshida, Tokuyuki, Inoue, Takao, Tanaka, Aki, Ito, Takashi, Hirose, Yutaka, Ohkuma, Yoshiaki
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container_title Genes to cells : devoted to molecular & cellular mechanisms
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creator Yamamoto, Seiji
Hagihara, Tomoko
Horiuchi, Yoshiyuki
Okui, Akira
Wani, Shotaro
Yoshida, Tokuyuki
Inoue, Takao
Tanaka, Aki
Ito, Takashi
Hirose, Yutaka
Ohkuma, Yoshiaki
description In eukaryotes, the Mediator complex has important roles in regulation of transcription by RNA polymerase II. Mediator is a large complex with more than 20 subunits that form head, middle, tail and CDK/cyclin modules. Among them, CDK8 and/or CDK19 (CDK8/19), and their counterpart cyclin C, form the CDK/cyclin module together with Mediator subunits MED12 and MED13. Despite evidences of both activation and repression, the precise functional roles of CDK8/19 in transcription are still elusive. Our previous results indicate that CDK8/19 recruits epigenetic regulators to repress immunoresponse genes. Here, this study focused on Toll-like receptors (TLRs), which exert innate immune responses through recognition of pathogen-associated molecular patterns and examined the functional roles of CDK8/19. As a result, CDK8/19 regulated transcription of inflammatory genes on stimulation of TLR9 in myeloma-derived RPMI8226 cells, which led to expression of inflammation-associated genes such as IL8,IL10,PTX3 and CCL2. Mediator subunits CDK8/19 and MED1, inflammation-related transcriptional activator NF-[kappa]B and C/EBP[beta], and general transcription factors TFIIE and TFIIB colocalized at the promoter regions of these genes under this condition. Our results show that CDK8/19 positively regulates inflammatory gene transcription in cooperation with NF-[kappa]B and C/EBP[beta] on stimulation of TLR9.
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subjects Cooperation
Cyclin-dependent kinases
Genes
Kinases
title Mediator cyclin-dependent kinases upregulate transcription of inflammatory genes in cooperation with NF-[kappa]B and C/EBP[beta] on stimulation of Toll-like receptor 9
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