Measurement and feature analysis of absorption spectra of four algal species
Two methods for particulate pigments （i.e., quantitative filter technique, QFT, and in vivo measurement, InVivo, respectively） and two methods for dissolved pigments （i.e., Acetone Extracts, AceEx, and high-performance liquid chromatography, HPLC, respectively） were used to obtain the optical absorp...
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| Veröffentlicht in: | Chinese journal of oceanology and limnology 2017-03, Vol.35 (2), p.350-359 |
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| creator | 朱建华 周虹丽 韩冰 李铜基 |
| description | Two methods for particulate pigments （i.e., quantitative filter technique, QFT, and in vivo measurement, InVivo, respectively） and two methods for dissolved pigments （i.e., Acetone Extracts, AceEx, and high-performance liquid chromatography, HPLC, respectively） were used to obtain the optical absorption coefficient spectra for cultures of four typical algal species. Through normalization and analysis of the spectra, it is shown that （1） the four methods are able to measure optical absorption spectra of particulate and/or dissolved pigments; （2） that the optical absorption spectra of particulate and dissolved pigments were consistent in terms of the peak position in the blue wavelength, and the difference of the peak position in the near infrared wavelength was -10 nm between each other; and （3） that the leveling effect of the absorption spectra of particulate pigments was significant. These four methods can all effectively measure the absorption coefficients of phytoplankton pigments, while each one has its unique advantages in different applications. Therefore, appropriate method should be carefully selected for various application due to their intrinsic difference. |
| doi_str_mv | 10.1007/s00343-016-5321-9 |
| format | Article |
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Through normalization and analysis of the spectra, it is shown that （1） the four methods are able to measure optical absorption spectra of particulate and/or dissolved pigments; （2） that the optical absorption spectra of particulate and dissolved pigments were consistent in terms of the peak position in the blue wavelength, and the difference of the peak position in the near infrared wavelength was -10 nm between each other; and （3） that the leveling effect of the absorption spectra of particulate pigments was significant. These four methods can all effectively measure the absorption coefficients of phytoplankton pigments, while each one has its unique advantages in different applications. Therefore, appropriate method should be carefully selected for various application due to their intrinsic difference.</description><identifier>ISSN: 0254-4059</identifier><identifier>ISSN: 2096-5508</identifier><identifier>EISSN: 1993-5005</identifier><identifier>EISSN: 2523-3521</identifier><identifier>DOI: 10.1007/s00343-016-5321-9</identifier><language>eng</language><publisher>Heidelberg: Science Press</publisher><subject>Absorption ; Absorption coefficient ; Absorption spectra ; Absorptivity ; Acetone ; Algae ; Biology ; Cellular biology ; Chromatography ; Coefficients ; Earth and Environmental Science ; Earth Sciences ; High performance liquid chromatography ; HPLC ; In vivo methods and tests ; Light ; Liquid chromatography ; Marine ; Oceanography ; Photosynthesis ; Phytoplankton ; Pigments ; Spectra ; Wavelength ; 丙酮提取物 ; 光吸收谱 ; 光学吸收 ; 光谱测量 ; 应用程序 ; 特征 ; 藻类培养 ; 高效液相色谱法</subject><ispartof>Chinese journal of oceanology and limnology, 2017-03, Vol.35 (2), p.350-359</ispartof><rights>Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2017</rights><rights>Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-1669040291c541889e18ac0d3ccfa5a38cae2ae6d026df5fbb314371c0c87e7b3</citedby><cites>FETCH-LOGICAL-c376t-1669040291c541889e18ac0d3ccfa5a38cae2ae6d026df5fbb314371c0c87e7b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84119X/84119X.jpg</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1874628175/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1874628175?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,777,781,21369,21370,21371,21372,23237,27905,27906,33511,33684,33725,33986,34295,43640,43768,43786,43934,44048,64364,64368,72218,73853,74032,74051,74222,74339</link.rule.ids></links><search><creatorcontrib>朱建华 周虹丽 韩冰 李铜基</creatorcontrib><title>Measurement and feature analysis of absorption spectra of four algal species</title><title>Chinese journal of oceanology and limnology</title><addtitle>Chin. J. Ocean. Limnol</addtitle><addtitle>Chinese Journal of Oceanology and Limnology</addtitle><description>Two methods for particulate pigments （i.e., quantitative filter technique, QFT, and in vivo measurement, InVivo, respectively） and two methods for dissolved pigments （i.e., Acetone Extracts, AceEx, and high-performance liquid chromatography, HPLC, respectively） were used to obtain the optical absorption coefficient spectra for cultures of four typical algal species. Through normalization and analysis of the spectra, it is shown that （1） the four methods are able to measure optical absorption spectra of particulate and/or dissolved pigments; （2） that the optical absorption spectra of particulate and dissolved pigments were consistent in terms of the peak position in the blue wavelength, and the difference of the peak position in the near infrared wavelength was -10 nm between each other; and （3） that the leveling effect of the absorption spectra of particulate pigments was significant. These four methods can all effectively measure the absorption coefficients of phytoplankton pigments, while each one has its unique advantages in different applications. Therefore, appropriate method should be carefully selected for various application due to their intrinsic difference.</description><subject>Absorption</subject><subject>Absorption coefficient</subject><subject>Absorption spectra</subject><subject>Absorptivity</subject><subject>Acetone</subject><subject>Algae</subject><subject>Biology</subject><subject>Cellular biology</subject><subject>Chromatography</subject><subject>Coefficients</subject><subject>Earth and Environmental Science</subject><subject>Earth Sciences</subject><subject>High performance liquid chromatography</subject><subject>HPLC</subject><subject>In vivo methods and tests</subject><subject>Light</subject><subject>Liquid chromatography</subject><subject>Marine</subject><subject>Oceanography</subject><subject>Photosynthesis</subject><subject>Phytoplankton</subject><subject>Pigments</subject><subject>Spectra</subject><subject>Wavelength</subject><subject>丙酮提取物</subject><subject>光吸收谱</subject><subject>光学吸收</subject><subject>光谱测量</subject><subject>应用程序</subject><subject>特征</subject><subject>藻类培养</subject><subject>高效液相色谱法</subject><issn>0254-4059</issn><issn>2096-5508</issn><issn>1993-5005</issn><issn>2523-3521</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kLlOw0AQhlcIJELgAegsaGgMM967RBGXFEQD9Wq9XgdHjp3s2gVvz-YQQhRUc-j75_gJuUS4RQB5FwEoozmgyDktMNdHZIJa05wD8GMygYKznAHXp-QsxmWiNQM9IfNXb-MY_Mp3Q2a7Kqu9HVKdctt-xSZmfZ3ZMvZhPTR9l8W1d0Ow227djyGz7cK2u27j4zk5qW0b_cUhTsnH48P77Dmfvz29zO7nuaNSDDkKoYFBodFxhkppj8o6qKhzteWWKmd9Yb2ooBBVzeuypMioRAdOSS9LOiU3-7nr0G9GHwezaqLzbWs734_RoJJSccYES-j1H3SZzk6_7SgmCoWSJwr3lAt9jMHXZh2alQ1fBsFs_TV7f03y12z9NTppir0mJrZb-PBr8j-iq8Oiz75bbJLuZ5OQyBBBSfoNfNGITw</recordid><startdate>20170301</startdate><enddate>20170301</enddate><creator>朱建华 周虹丽 韩冰 李铜基</creator><general>Science Press</general><general>Springer Nature B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7QL</scope><scope>7SN</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7XB</scope><scope>88I</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>GNUQQ</scope><scope>H96</scope><scope>HCIFZ</scope><scope>L.G</scope><scope>M2P</scope><scope>M7N</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20170301</creationdate><title>Measurement and feature analysis of absorption spectra of four algal species</title><author>朱建华 周虹丽 韩冰 李铜基</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-1669040291c541889e18ac0d3ccfa5a38cae2ae6d026df5fbb314371c0c87e7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Absorption</topic><topic>Absorption coefficient</topic><topic>Absorption spectra</topic><topic>Absorptivity</topic><topic>Acetone</topic><topic>Algae</topic><topic>Biology</topic><topic>Cellular biology</topic><topic>Chromatography</topic><topic>Coefficients</topic><topic>Earth and Environmental Science</topic><topic>Earth Sciences</topic><topic>High performance liquid chromatography</topic><topic>HPLC</topic><topic>In vivo methods and tests</topic><topic>Light</topic><topic>Liquid chromatography</topic><topic>Marine</topic><topic>Oceanography</topic><topic>Photosynthesis</topic><topic>Phytoplankton</topic><topic>Pigments</topic><topic>Spectra</topic><topic>Wavelength</topic><topic>丙酮提取物</topic><topic>光吸收谱</topic><topic>光学吸收</topic><topic>光谱测量</topic><topic>应用程序</topic><topic>特征</topic><topic>藻类培养</topic><topic>高效液相色谱法</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>朱建华 周虹丽 韩冰 李铜基</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water 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J. Ocean. Limnol</stitle><addtitle>Chinese Journal of Oceanology and Limnology</addtitle><date>2017-03-01</date><risdate>2017</risdate><volume>35</volume><issue>2</issue><spage>350</spage><epage>359</epage><pages>350-359</pages><issn>0254-4059</issn><issn>2096-5508</issn><eissn>1993-5005</eissn><eissn>2523-3521</eissn><abstract>Two methods for particulate pigments （i.e., quantitative filter technique, QFT, and in vivo measurement, InVivo, respectively） and two methods for dissolved pigments （i.e., Acetone Extracts, AceEx, and high-performance liquid chromatography, HPLC, respectively） were used to obtain the optical absorption coefficient spectra for cultures of four typical algal species. Through normalization and analysis of the spectra, it is shown that （1） the four methods are able to measure optical absorption spectra of particulate and/or dissolved pigments; （2） that the optical absorption spectra of particulate and dissolved pigments were consistent in terms of the peak position in the blue wavelength, and the difference of the peak position in the near infrared wavelength was -10 nm between each other; and （3） that the leveling effect of the absorption spectra of particulate pigments was significant. These four methods can all effectively measure the absorption coefficients of phytoplankton pigments, while each one has its unique advantages in different applications. Therefore, appropriate method should be carefully selected for various application due to their intrinsic difference.</abstract><cop>Heidelberg</cop><pub>Science Press</pub><doi>10.1007/s00343-016-5321-9</doi><tpages>10</tpages></addata></record> |
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| subjects | Absorption Absorption coefficient Absorption spectra Absorptivity Acetone Algae Biology Cellular biology Chromatography Coefficients Earth and Environmental Science Earth Sciences High performance liquid chromatography HPLC In vivo methods and tests Light Liquid chromatography Marine Oceanography Photosynthesis Phytoplankton Pigments Spectra Wavelength 丙酮提取物 光吸收谱 光学吸收 光谱测量 应用程序 特征 藻类培养 高效液相色谱法 |
| title | Measurement and feature analysis of absorption spectra of four algal species |
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