Community Structure of Endophytic Bacteria in Chestnut, Castanea mollissima

Community Structure of Endophytic Bacteria in Chestnut, Castanea mollissima

[ Objective] The paper was to detect the species of endophytic bacteria in Chestnut （Castanea mollissima）. [ Method ] The 16S rDNA-V4 region of endophytic bacteria in chestnut （sample name BL） was sequenced by Illumina MiSeq high-throughput sequencing technology. The number of sequences and op- erat...

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Veröffentlicht in:Plant Diseases and Pests(植物病虫害研究：英文版) 2016-08, Vol.7 (4), p.1-5
Hauptverfasser: Zebin, Chen, Ring, Li, Tiyuan, Xia, Dingkang, Wang, Lei, Yu, Shengguang, Xu, Jiani, Liu, Yongfu, Zhang
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container_title Plant Diseases and Pests(植物病虫害研究：英文版)
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creator Zebin, Chen
Ring, Li
Tiyuan, Xia
Dingkang, Wang
Lei, Yu
Shengguang, Xu
Jiani, Liu
Yongfu, Zhang
description [ Objective] The paper was to detect the species of endophytic bacteria in Chestnut （Castanea mollissima）. [ Method ] The 16S rDNA-V4 region of endophytic bacteria in chestnut （sample name BL） was sequenced by Illumina MiSeq high-throughput sequencing technology. The number of sequences and op- erational taxonomic units （OTUs） for each sample was sorted and calculated using Qiime and Mothur software. The abundance and α-diversity of species were analyzed. [ Result] The number of effective sequences and OTUs were 21 167/191. The rarefaction curves showed that adequate sampling was achieved, and the number of OTUs was close to saturation. The endophytic bacteria of chestnut belonged to 10 genera, including Koribacter （3.48%） , Solibacter （2.59%） , Bra- d~hizobium （ 3. 57% ）, Rhodoplanes （ 7. 41% ）, Methylobacterium （ 4. 20% ）, Agrobacterium （ 19. 73% ）, Kaistobacter （ 2. 05% ）, Sphingomonas （18.66%）, Acidovorax （35.98%）, and Methylibium （2.32%）. The dominant species were Acidovorax, Agrobacterium and Sphingomonas. [ Conclusion] Illumina high-throughput sequencing technology provided more accurate and scientific data resources for the study of endophytic bacteria in chestnut.
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[ Method ] The 16S rDNA-V4 region of endophytic bacteria in chestnut （sample name BL） was sequenced by Illumina MiSeq high-throughput sequencing technology. The number of sequences and op- erational taxonomic units （OTUs） for each sample was sorted and calculated using Qiime and Mothur software. The abundance and α-diversity of species were analyzed. [ Result] The number of effective sequences and OTUs were 21 167/191. The rarefaction curves showed that adequate sampling was achieved, and the number of OTUs was close to saturation. The endophytic bacteria of chestnut belonged to 10 genera, including Koribacter （3.48%） , Solibacter （2.59%） , Bra- d~hizobium （ 3. 57% ）, Rhodoplanes （ 7. 41% ）, Methylobacterium （ 4. 20% ）, Agrobacterium （ 19. 73% ）, Kaistobacter （ 2. 05% ）, Sphingomonas （18.66%）, Acidovorax （35.98%）, and Methylibium （2.32%）. The dominant species were Acidovorax, Agrobacterium and Sphingomonas. 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title Community Structure of Endophytic Bacteria in Chestnut, Castanea mollissima
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