Community Structure of Endophytic Bacteria in Chestnut, Castanea mollissima
[ Objective] The paper was to detect the species of endophytic bacteria in Chestnut (Castanea mollissima). [ Method ] The 16S rDNA-V4 region of endophytic bacteria in chestnut (sample name BL) was sequenced by Illumina MiSeq high-throughput sequencing technology. The number of sequences and op- erat...
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Veröffentlicht in: | Plant Diseases and Pests(植物病虫害研究:英文版) 2016-08, Vol.7 (4), p.1-5 |
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Sprache: | eng |
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Zusammenfassung: | [ Objective] The paper was to detect the species of endophytic bacteria in Chestnut (Castanea mollissima). [ Method ] The 16S rDNA-V4 region of endophytic bacteria in chestnut (sample name BL) was sequenced by Illumina MiSeq high-throughput sequencing technology. The number of sequences and op- erational taxonomic units (OTUs) for each sample was sorted and calculated using Qiime and Mothur software. The abundance and α-diversity of species were analyzed. [ Result] The number of effective sequences and OTUs were 21 167/191. The rarefaction curves showed that adequate sampling was achieved, and the number of OTUs was close to saturation. The endophytic bacteria of chestnut belonged to 10 genera, including Koribacter (3.48%) , Solibacter (2.59%) , Bra- d~hizobium ( 3. 57% ), Rhodoplanes ( 7. 41% ), Methylobacterium ( 4. 20% ), Agrobacterium ( 19. 73% ), Kaistobacter ( 2. 05% ), Sphingomonas (18.66%), Acidovorax (35.98%), and Methylibium (2.32%). The dominant species were Acidovorax, Agrobacterium and Sphingomonas. [ Conclusion] Illumina high-throughput sequencing technology provided more accurate and scientific data resources for the study of endophytic bacteria in chestnut. |
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ISSN: | 2152-3932 |