Production of the Functional Trisaccharide 1-Kestose from Cane Sugar Molasses Using Aspergillus japonicus [beta]-Fructofuranosidase
We report the production of the functional trisaccharide 1-kestose, O-[beta]-d-fructofuranosyl-(2[arrow right]1)-[beta]-d-fructofuranosyl [alpha]-d-glucopyranoside, by [beta]-fructofuranosidase from Aspergillus japonicus using sugar cane molasses as substrate. Sucrose in cane sugar molasses acted as...
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| Veröffentlicht in: | Current microbiology 2017-01, Vol.74 (1), p.145 |
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| creator | Hirabayashi, Katsuki Kondo, Nobuhiro Toyota, Hiroshi Hayashi, Sachio |
| description | We report the production of the functional trisaccharide 1-kestose, O-[beta]-d-fructofuranosyl-(2[arrow right]1)-[beta]-d-fructofuranosyl [alpha]-d-glucopyranoside, by [beta]-fructofuranosidase from Aspergillus japonicus using sugar cane molasses as substrate. Sucrose in cane sugar molasses acted as a fructosyl donor and acceptor for the enzyme. The tetrasaccharide nystose, O-[beta]-d-fructofuranosyl-(2[arrow right]1)-[beta]-d-fructofuranosyl-(2[arrow right]1)-[beta]-d-fructofuranosyl [alpha]-d-glucopyranoside, was produced from 1-kestose. Cane sugar molasses mixed with water provided a better substrate solution for [beta]-fructofuranosidase compared to undiluted molasses due to the high concentration of product inhibitors such as glucose and fructose in molasses. The maximum concentration of 1-kestose obtained was 84.9 mg/ml and the maximum production efficiency was 32.3% after 24 h reaction at 40 °C. The maximum efficiency of combined fructo-oligosaccharide (1-kestose and nystose) production was 40.6%. 1-Kestose was therefore produced via a fructosyl-transfer reaction catalyzed by [beta]-fructofuranosidase from A. japonicus. |
| doi_str_mv | 10.1007/s00284-016-1154-1 |
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Sucrose in cane sugar molasses acted as a fructosyl donor and acceptor for the enzyme. The tetrasaccharide nystose, O-[beta]-d-fructofuranosyl-(2[arrow right]1)-[beta]-d-fructofuranosyl-(2[arrow right]1)-[beta]-d-fructofuranosyl [alpha]-d-glucopyranoside, was produced from 1-kestose. Cane sugar molasses mixed with water provided a better substrate solution for [beta]-fructofuranosidase compared to undiluted molasses due to the high concentration of product inhibitors such as glucose and fructose in molasses. The maximum concentration of 1-kestose obtained was 84.9 mg/ml and the maximum production efficiency was 32.3% after 24 h reaction at 40 °C. The maximum efficiency of combined fructo-oligosaccharide (1-kestose and nystose) production was 40.6%. 1-Kestose was therefore produced via a fructosyl-transfer reaction catalyzed by [beta]-fructofuranosidase from A. japonicus.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-016-1154-1</identifier><language>eng</language><publisher>New York: Springer Nature B.V</publisher><subject>Enzymes ; Sugar ; Sugarcane</subject><ispartof>Current microbiology, 2017-01, Vol.74 (1), p.145</ispartof><rights>Current Microbiology is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Hirabayashi, Katsuki</creatorcontrib><creatorcontrib>Kondo, Nobuhiro</creatorcontrib><creatorcontrib>Toyota, Hiroshi</creatorcontrib><creatorcontrib>Hayashi, Sachio</creatorcontrib><title>Production of the Functional Trisaccharide 1-Kestose from Cane Sugar Molasses Using Aspergillus japonicus [beta]-Fructofuranosidase</title><title>Current microbiology</title><description>We report the production of the functional trisaccharide 1-kestose, O-[beta]-d-fructofuranosyl-(2[arrow right]1)-[beta]-d-fructofuranosyl [alpha]-d-glucopyranoside, by [beta]-fructofuranosidase from Aspergillus japonicus using sugar cane molasses as substrate. Sucrose in cane sugar molasses acted as a fructosyl donor and acceptor for the enzyme. The tetrasaccharide nystose, O-[beta]-d-fructofuranosyl-(2[arrow right]1)-[beta]-d-fructofuranosyl-(2[arrow right]1)-[beta]-d-fructofuranosyl [alpha]-d-glucopyranoside, was produced from 1-kestose. Cane sugar molasses mixed with water provided a better substrate solution for [beta]-fructofuranosidase compared to undiluted molasses due to the high concentration of product inhibitors such as glucose and fructose in molasses. The maximum concentration of 1-kestose obtained was 84.9 mg/ml and the maximum production efficiency was 32.3% after 24 h reaction at 40 °C. The maximum efficiency of combined fructo-oligosaccharide (1-kestose and nystose) production was 40.6%. 1-Kestose was therefore produced via a fructosyl-transfer reaction catalyzed by [beta]-fructofuranosidase from A. japonicus.</description><subject>Enzymes</subject><subject>Sugar</subject><subject>Sugarcane</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNjstKxEAQRRtRMD4-wF2B69aqvEyWMhiEQRAcVyJDmXQyHWJ37Ep_gT9uED_A1b0XDpyr1BXhDSHe3QpiWuUaqdRERa7pSCWUZ6nGuqZjlWCWZ7oqCzpVZyIjIqU1UqK-n4PvYrtY78D3sBwMNNH9bp5gF6xw2x442M4A6a2RxYuBPvhP2LAz8BIHDvDkJxYxAq9i3QD3Mpsw2GmKAiPP3tl2bW8fZuF33YRV5_sY2HmxHYu5UCc9T2Iu__JcXTcPu82jnoP_iqtyP_oY1j-yp6oosS4zKrL_UT873Vjt</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Hirabayashi, Katsuki</creator><creator>Kondo, Nobuhiro</creator><creator>Toyota, Hiroshi</creator><creator>Hayashi, Sachio</creator><general>Springer Nature B.V</general><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20170101</creationdate><title>Production of the Functional Trisaccharide 1-Kestose from Cane Sugar Molasses Using Aspergillus japonicus [beta]-Fructofuranosidase</title><author>Hirabayashi, Katsuki ; 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Sucrose in cane sugar molasses acted as a fructosyl donor and acceptor for the enzyme. The tetrasaccharide nystose, O-[beta]-d-fructofuranosyl-(2[arrow right]1)-[beta]-d-fructofuranosyl-(2[arrow right]1)-[beta]-d-fructofuranosyl [alpha]-d-glucopyranoside, was produced from 1-kestose. Cane sugar molasses mixed with water provided a better substrate solution for [beta]-fructofuranosidase compared to undiluted molasses due to the high concentration of product inhibitors such as glucose and fructose in molasses. The maximum concentration of 1-kestose obtained was 84.9 mg/ml and the maximum production efficiency was 32.3% after 24 h reaction at 40 °C. The maximum efficiency of combined fructo-oligosaccharide (1-kestose and nystose) production was 40.6%. 1-Kestose was therefore produced via a fructosyl-transfer reaction catalyzed by [beta]-fructofuranosidase from A. japonicus.</abstract><cop>New York</cop><pub>Springer Nature B.V</pub><doi>10.1007/s00284-016-1154-1</doi></addata></record> |
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| title | Production of the Functional Trisaccharide 1-Kestose from Cane Sugar Molasses Using Aspergillus japonicus [beta]-Fructofuranosidase |
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