Effects of antioxidants, catalase and [alpha]-tocopherol on cell viability and oxidative stress variables in frozen-thawed mice spermatogonial stem cells
Cryopreservation of spermatogonial stem cells is considered as a useful procedure for preserving fertility in children with testis cancer. SSCs were isolated from testes mice, and then antioxidant was added to the freezing medium. The Bax expression level in antioxidant groups was significantly (P [...
Gespeichert in:
| Veröffentlicht in: | Artificial cells, nanomedicine, and biotechnology nanomedicine, and biotechnology, 2017-02, Vol.45 (1), p.63 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | 63 |
| container_title | Artificial cells, nanomedicine, and biotechnology |
| container_volume | 45 |
| creator | Aliakbari, Freshte Sedighi Gilani, Mohamad Ali Yazdekhasti, Hossein Koruji, Morteza Asgari, Hamid Reza Baazm, Maryam Izadyar, Fariborz Kharrazi Nejad, Ebrahim Khanezad, Maryam Abbasi, Mehdi |
| description | Cryopreservation of spermatogonial stem cells is considered as a useful procedure for preserving fertility in children with testis cancer. SSCs were isolated from testes mice, and then antioxidant was added to the freezing medium. The Bax expression level in antioxidant groups was significantly (P [less-than or equal to] 0.05) lower than the control group and a significant rise of Bcl2 expression was detected in the antioxidant groups. ROS production with antioxidant was significantly lower compared with the control group. Cryopreservation with the addition of the antioxidants can help increase the number of SSCs and improve the quality and viability of these cells after cryopreservation. |
| doi_str_mv | 10.3109/21691401.2016.1138491 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_1848114756</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4276555991</sourcerecordid><originalsourceid>FETCH-proquest_journals_18481147563</originalsourceid><addsrcrecordid>eNqNjN1KxDAQhYMouOg-ghDw1tbMNtttr2XFB_BCEFnGdmqzpEnNZOvPm_i2xiJee27O4cx3RogLUHkBqr5eQVmDVpCvFJQ5QFHpGo7E4qfPQMPD8V9WcCqWzHuVVEG5WeuF-Np2HTWRpe8kumj8u2mT85VsMKJFplS38hHt2ONTFn3jx56Ct9I72ZC1cjL4bKyJHzM476OZSHIMxCwnDAmwxNI42QX_SS6LPb5RKwfTJGykMGD0L94ZtGlFw_yXz8VJh5Zp-etn4vJ2e39zl43Bvx6I427vD8Gl0w4qXQHozbos_kd9A9z5Yv8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1848114756</pqid></control><display><type>article</type><title>Effects of antioxidants, catalase and [alpha]-tocopherol on cell viability and oxidative stress variables in frozen-thawed mice spermatogonial stem cells</title><source>Alma/SFX Local Collection</source><creator>Aliakbari, Freshte ; Sedighi Gilani, Mohamad Ali ; Yazdekhasti, Hossein ; Koruji, Morteza ; Asgari, Hamid Reza ; Baazm, Maryam ; Izadyar, Fariborz ; Kharrazi Nejad, Ebrahim ; Khanezad, Maryam ; Abbasi, Mehdi</creator><creatorcontrib>Aliakbari, Freshte ; Sedighi Gilani, Mohamad Ali ; Yazdekhasti, Hossein ; Koruji, Morteza ; Asgari, Hamid Reza ; Baazm, Maryam ; Izadyar, Fariborz ; Kharrazi Nejad, Ebrahim ; Khanezad, Maryam ; Abbasi, Mehdi</creatorcontrib><description>Cryopreservation of spermatogonial stem cells is considered as a useful procedure for preserving fertility in children with testis cancer. SSCs were isolated from testes mice, and then antioxidant was added to the freezing medium. The Bax expression level in antioxidant groups was significantly (P [less-than or equal to] 0.05) lower than the control group and a significant rise of Bcl2 expression was detected in the antioxidant groups. ROS production with antioxidant was significantly lower compared with the control group. Cryopreservation with the addition of the antioxidants can help increase the number of SSCs and improve the quality and viability of these cells after cryopreservation.</description><identifier>ISSN: 2169-1401</identifier><identifier>EISSN: 2169-141X</identifier><identifier>DOI: 10.3109/21691401.2016.1138491</identifier><language>eng</language><publisher>Abingdon: Taylor & Francis Ltd</publisher><subject>Antioxidants ; Catalase ; Cell viability ; Cryopreservation ; Fertility ; Freezing ; Oxidative stress ; Stem cell transplantation ; Stem cells ; Testes ; Tocopherol</subject><ispartof>Artificial cells, nanomedicine, and biotechnology, 2017-02, Vol.45 (1), p.63</ispartof><rights>2016 Informa UK Limited, trading as Taylor & Francis Group</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Aliakbari, Freshte</creatorcontrib><creatorcontrib>Sedighi Gilani, Mohamad Ali</creatorcontrib><creatorcontrib>Yazdekhasti, Hossein</creatorcontrib><creatorcontrib>Koruji, Morteza</creatorcontrib><creatorcontrib>Asgari, Hamid Reza</creatorcontrib><creatorcontrib>Baazm, Maryam</creatorcontrib><creatorcontrib>Izadyar, Fariborz</creatorcontrib><creatorcontrib>Kharrazi Nejad, Ebrahim</creatorcontrib><creatorcontrib>Khanezad, Maryam</creatorcontrib><creatorcontrib>Abbasi, Mehdi</creatorcontrib><title>Effects of antioxidants, catalase and [alpha]-tocopherol on cell viability and oxidative stress variables in frozen-thawed mice spermatogonial stem cells</title><title>Artificial cells, nanomedicine, and biotechnology</title><description>Cryopreservation of spermatogonial stem cells is considered as a useful procedure for preserving fertility in children with testis cancer. SSCs were isolated from testes mice, and then antioxidant was added to the freezing medium. The Bax expression level in antioxidant groups was significantly (P [less-than or equal to] 0.05) lower than the control group and a significant rise of Bcl2 expression was detected in the antioxidant groups. ROS production with antioxidant was significantly lower compared with the control group. Cryopreservation with the addition of the antioxidants can help increase the number of SSCs and improve the quality and viability of these cells after cryopreservation.</description><subject>Antioxidants</subject><subject>Catalase</subject><subject>Cell viability</subject><subject>Cryopreservation</subject><subject>Fertility</subject><subject>Freezing</subject><subject>Oxidative stress</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Testes</subject><subject>Tocopherol</subject><issn>2169-1401</issn><issn>2169-141X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqNjN1KxDAQhYMouOg-ghDw1tbMNtttr2XFB_BCEFnGdmqzpEnNZOvPm_i2xiJee27O4cx3RogLUHkBqr5eQVmDVpCvFJQ5QFHpGo7E4qfPQMPD8V9WcCqWzHuVVEG5WeuF-Np2HTWRpe8kumj8u2mT85VsMKJFplS38hHt2ONTFn3jx56Ct9I72ZC1cjL4bKyJHzM476OZSHIMxCwnDAmwxNI42QX_SS6LPb5RKwfTJGykMGD0L94ZtGlFw_yXz8VJh5Zp-etn4vJ2e39zl43Bvx6I427vD8Gl0w4qXQHozbos_kd9A9z5Yv8</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Aliakbari, Freshte</creator><creator>Sedighi Gilani, Mohamad Ali</creator><creator>Yazdekhasti, Hossein</creator><creator>Koruji, Morteza</creator><creator>Asgari, Hamid Reza</creator><creator>Baazm, Maryam</creator><creator>Izadyar, Fariborz</creator><creator>Kharrazi Nejad, Ebrahim</creator><creator>Khanezad, Maryam</creator><creator>Abbasi, Mehdi</creator><general>Taylor & Francis Ltd</general><scope/></search><sort><creationdate>20170201</creationdate><title>Effects of antioxidants, catalase and [alpha]-tocopherol on cell viability and oxidative stress variables in frozen-thawed mice spermatogonial stem cells</title><author>Aliakbari, Freshte ; Sedighi Gilani, Mohamad Ali ; Yazdekhasti, Hossein ; Koruji, Morteza ; Asgari, Hamid Reza ; Baazm, Maryam ; Izadyar, Fariborz ; Kharrazi Nejad, Ebrahim ; Khanezad, Maryam ; Abbasi, Mehdi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_18481147563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Antioxidants</topic><topic>Catalase</topic><topic>Cell viability</topic><topic>Cryopreservation</topic><topic>Fertility</topic><topic>Freezing</topic><topic>Oxidative stress</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Testes</topic><topic>Tocopherol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aliakbari, Freshte</creatorcontrib><creatorcontrib>Sedighi Gilani, Mohamad Ali</creatorcontrib><creatorcontrib>Yazdekhasti, Hossein</creatorcontrib><creatorcontrib>Koruji, Morteza</creatorcontrib><creatorcontrib>Asgari, Hamid Reza</creatorcontrib><creatorcontrib>Baazm, Maryam</creatorcontrib><creatorcontrib>Izadyar, Fariborz</creatorcontrib><creatorcontrib>Kharrazi Nejad, Ebrahim</creatorcontrib><creatorcontrib>Khanezad, Maryam</creatorcontrib><creatorcontrib>Abbasi, Mehdi</creatorcontrib><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aliakbari, Freshte</au><au>Sedighi Gilani, Mohamad Ali</au><au>Yazdekhasti, Hossein</au><au>Koruji, Morteza</au><au>Asgari, Hamid Reza</au><au>Baazm, Maryam</au><au>Izadyar, Fariborz</au><au>Kharrazi Nejad, Ebrahim</au><au>Khanezad, Maryam</au><au>Abbasi, Mehdi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of antioxidants, catalase and [alpha]-tocopherol on cell viability and oxidative stress variables in frozen-thawed mice spermatogonial stem cells</atitle><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle><date>2017-02-01</date><risdate>2017</risdate><volume>45</volume><issue>1</issue><spage>63</spage><pages>63-</pages><issn>2169-1401</issn><eissn>2169-141X</eissn><abstract>Cryopreservation of spermatogonial stem cells is considered as a useful procedure for preserving fertility in children with testis cancer. SSCs were isolated from testes mice, and then antioxidant was added to the freezing medium. The Bax expression level in antioxidant groups was significantly (P [less-than or equal to] 0.05) lower than the control group and a significant rise of Bcl2 expression was detected in the antioxidant groups. ROS production with antioxidant was significantly lower compared with the control group. Cryopreservation with the addition of the antioxidants can help increase the number of SSCs and improve the quality and viability of these cells after cryopreservation.</abstract><cop>Abingdon</cop><pub>Taylor & Francis Ltd</pub><doi>10.3109/21691401.2016.1138491</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2169-1401 |
| ispartof | Artificial cells, nanomedicine, and biotechnology, 2017-02, Vol.45 (1), p.63 |
| issn | 2169-1401 2169-141X |
| language | eng |
| recordid | cdi_proquest_journals_1848114756 |
| source | Alma/SFX Local Collection |
| subjects | Antioxidants Catalase Cell viability Cryopreservation Fertility Freezing Oxidative stress Stem cell transplantation Stem cells Testes Tocopherol |
| title | Effects of antioxidants, catalase and [alpha]-tocopherol on cell viability and oxidative stress variables in frozen-thawed mice spermatogonial stem cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T06%3A55%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20antioxidants,%20catalase%20and%20%5Balpha%5D-tocopherol%20on%20cell%20viability%20and%20oxidative%20stress%20variables%20in%20frozen-thawed%20mice%20spermatogonial%20stem%20cells&rft.jtitle=Artificial%20cells,%20nanomedicine,%20and%20biotechnology&rft.au=Aliakbari,%20Freshte&rft.date=2017-02-01&rft.volume=45&rft.issue=1&rft.spage=63&rft.pages=63-&rft.issn=2169-1401&rft.eissn=2169-141X&rft_id=info:doi/10.3109/21691401.2016.1138491&rft_dat=%3Cproquest%3E4276555991%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1848114756&rft_id=info:pmid/&rfr_iscdi=true |