Quantification of Triacylglycerol Positional Isomers in Rat Milk
The absolute amount of triacylglycerol (TAG) positional isomers was analyzed in rat milk fat, a representative of non-ruminant milk fat, using a HPLC-UV-atmospheric pressure chemical ionization-MS/MS system equipped with an octacosyl silylation column or polymeric ODS column. TAGs consisting of two...
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Veröffentlicht in: | Journal of Oleo Science 2016, Vol.65(12), pp.977-983 |
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description | The absolute amount of triacylglycerol (TAG) positional isomers was analyzed in rat milk fat, a representative of non-ruminant milk fat, using a HPLC-UV-atmospheric pressure chemical ionization-MS/MS system equipped with an octacosyl silylation column or polymeric ODS column. TAGs consisting of two oleic acids (O) and one palmitic acid (P) were the most abundant. In particular, β-OPO, a TAG binding P at the β-position (sn-2) and two Os at the α-positions (sn-1/3), was prominent. The β-OPO content decreased over time, while a TAG consisting of two Ps and one capric acid, a medium-chain fatty acid, increased. TAGs consisting of two Ps and one docosahexaenoic acid were present in small amounts and decreased with time. These results indicated that the recombination of fatty acids in TAGs in milk fat occurs in the mother, and is thought to depend on the infant’s stage of growth, in response to their nutritional needs. It was also demonstrated that medium-chain fatty acids were mainly located at the α-position (sn-3), while Ps were mainly located at the β-position (sn-2). Therefore, the combination and binding positions of fatty acids of TAG are considered very important in infant nutrition. |
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TAGs consisting of two oleic acids (O) and one palmitic acid (P) were the most abundant. In particular, β-OPO, a TAG binding P at the β-position (sn-2) and two Os at the α-positions (sn-1/3), was prominent. The β-OPO content decreased over time, while a TAG consisting of two Ps and one capric acid, a medium-chain fatty acid, increased. TAGs consisting of two Ps and one docosahexaenoic acid were present in small amounts and decreased with time. These results indicated that the recombination of fatty acids in TAGs in milk fat occurs in the mother, and is thought to depend on the infant’s stage of growth, in response to their nutritional needs. It was also demonstrated that medium-chain fatty acids were mainly located at the α-position (sn-3), while Ps were mainly located at the β-position (sn-2). Therefore, the combination and binding positions of fatty acids of TAG are considered very important in infant nutrition.</description><identifier>ISSN: 1345-8957</identifier><identifier>EISSN: 1347-3352</identifier><identifier>DOI: 10.5650/jos.ess16115</identifier><identifier>PMID: 27829613</identifier><language>eng</language><publisher>Japan: Japan Oil Chemists' Society</publisher><subject>Animal fat ; Animals ; Binding ; Docosahexaenoic acid ; Fats - chemistry ; Fatty acids ; Fatty Acids - analysis ; infant nutrition ; Infants ; Isomers ; Milk ; Milk - chemistry ; milk fat ; Nutrition ; Oils & fats ; Palmitic acid ; quantification ; Rats ; Stereoisomerism ; Tags ; triacylglycerol positional isomer ; Triglycerides ; Triglycerides - analysis ; Triglycerides - chemistry</subject><ispartof>Journal of Oleo Science, 2016, Vol.65(12), pp.977-983</ispartof><rights>2016 by Japan Oil Chemists' Society</rights><rights>Copyright Japan Science and Technology Agency 2016</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c622t-b9f236c32a720edd23653a3dccb0fd7c28ce43cd9035322b99c2997657dd63083</citedby><cites>FETCH-LOGICAL-c622t-b9f236c32a720edd23653a3dccb0fd7c28ce43cd9035322b99c2997657dd63083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27829613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watanabe, Natsuko</creatorcontrib><creatorcontrib>Nagai, Toshiharu</creatorcontrib><creatorcontrib>Mizobe, Hoyo</creatorcontrib><creatorcontrib>Yoshinaga, Kazuaki</creatorcontrib><creatorcontrib>Yoshida, Akihiko</creatorcontrib><creatorcontrib>Kitamura, Yohei</creatorcontrib><creatorcontrib>Shimizu, Takashi</creatorcontrib><creatorcontrib>Beppu, Fumiaki</creatorcontrib><creatorcontrib>Gotoh, Naohiro</creatorcontrib><creatorcontrib>Department of Food Science and Technology</creatorcontrib><creatorcontrib>Tsukishima Foods Industry Co</creatorcontrib><creatorcontrib>Tokyo University of Marine Science and Technology</creatorcontrib><creatorcontrib>Morinaga Milk Industry Co</creatorcontrib><creatorcontrib>Ltd</creatorcontrib><title>Quantification of Triacylglycerol Positional Isomers in Rat Milk</title><title>Journal of Oleo Science</title><addtitle>J Oleo Sci</addtitle><description>The absolute amount of triacylglycerol (TAG) positional isomers was analyzed in rat milk fat, a representative of non-ruminant milk fat, using a HPLC-UV-atmospheric pressure chemical ionization-MS/MS system equipped with an octacosyl silylation column or polymeric ODS column. TAGs consisting of two oleic acids (O) and one palmitic acid (P) were the most abundant. In particular, β-OPO, a TAG binding P at the β-position (sn-2) and two Os at the α-positions (sn-1/3), was prominent. The β-OPO content decreased over time, while a TAG consisting of two Ps and one capric acid, a medium-chain fatty acid, increased. TAGs consisting of two Ps and one docosahexaenoic acid were present in small amounts and decreased with time. These results indicated that the recombination of fatty acids in TAGs in milk fat occurs in the mother, and is thought to depend on the infant’s stage of growth, in response to their nutritional needs. It was also demonstrated that medium-chain fatty acids were mainly located at the α-position (sn-3), while Ps were mainly located at the β-position (sn-2). Therefore, the combination and binding positions of fatty acids of TAG are considered very important in infant nutrition.</description><subject>Animal fat</subject><subject>Animals</subject><subject>Binding</subject><subject>Docosahexaenoic acid</subject><subject>Fats - chemistry</subject><subject>Fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>infant nutrition</subject><subject>Infants</subject><subject>Isomers</subject><subject>Milk</subject><subject>Milk - chemistry</subject><subject>milk fat</subject><subject>Nutrition</subject><subject>Oils & fats</subject><subject>Palmitic acid</subject><subject>quantification</subject><subject>Rats</subject><subject>Stereoisomerism</subject><subject>Tags</subject><subject>triacylglycerol positional isomer</subject><subject>Triglycerides</subject><subject>Triglycerides - analysis</subject><subject>Triglycerides - chemistry</subject><issn>1345-8957</issn><issn>1347-3352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUE1PAyEUJEbj982z2cSrq_AosNw0jVYTjR_RM6HAKitdFLaH_nvR2np5D5h584ZB6IjgM8YZPu9iPnM5E04I20C7hI5ETSmDzd8zqxvJxA7ay7nDuLwzsY12QDQgOaG76OJprvvBt97owce-im31krw2i_AWFsalGKrHmP0PpkN1m-PMpVz5vnrWQ3Xvw8cB2mp1yO7wr--j1-url_FNffcwuR1f3tWGAwz1VLZAuaGgBWBnbbkwqqk1ZopbKww0xo2osRJTRgGmUhqQUnAmrOUUN3QfnSx1P1P8mrs8qC7OUzGVFWlG5S9AGC6s0yXLpJhzcq36TH6m00IRrH7iKlNZreIq9OM_0fl05uyavMqnECZLQkFLRCH2wffuf7XtRAyuSAImXGHMGYHSqMJSiFIaCoJzYLIojZdKXR70m1uv0mnwJrhfX5ypMl7qyuAaNe86KdfTbwejlMs</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Watanabe, Natsuko</creator><creator>Nagai, Toshiharu</creator><creator>Mizobe, Hoyo</creator><creator>Yoshinaga, Kazuaki</creator><creator>Yoshida, Akihiko</creator><creator>Kitamura, Yohei</creator><creator>Shimizu, Takashi</creator><creator>Beppu, Fumiaki</creator><creator>Gotoh, Naohiro</creator><general>Japan Oil Chemists' Society</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>20161201</creationdate><title>Quantification of Triacylglycerol Positional Isomers in Rat Milk</title><author>Watanabe, Natsuko ; Nagai, Toshiharu ; Mizobe, Hoyo ; Yoshinaga, Kazuaki ; Yoshida, Akihiko ; Kitamura, Yohei ; Shimizu, Takashi ; Beppu, Fumiaki ; Gotoh, Naohiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c622t-b9f236c32a720edd23653a3dccb0fd7c28ce43cd9035322b99c2997657dd63083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animal fat</topic><topic>Animals</topic><topic>Binding</topic><topic>Docosahexaenoic acid</topic><topic>Fats - chemistry</topic><topic>Fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>infant nutrition</topic><topic>Infants</topic><topic>Isomers</topic><topic>Milk</topic><topic>Milk - chemistry</topic><topic>milk fat</topic><topic>Nutrition</topic><topic>Oils & fats</topic><topic>Palmitic acid</topic><topic>quantification</topic><topic>Rats</topic><topic>Stereoisomerism</topic><topic>Tags</topic><topic>triacylglycerol positional isomer</topic><topic>Triglycerides</topic><topic>Triglycerides - analysis</topic><topic>Triglycerides - chemistry</topic><toplevel>online_resources</toplevel><creatorcontrib>Watanabe, Natsuko</creatorcontrib><creatorcontrib>Nagai, Toshiharu</creatorcontrib><creatorcontrib>Mizobe, Hoyo</creatorcontrib><creatorcontrib>Yoshinaga, Kazuaki</creatorcontrib><creatorcontrib>Yoshida, Akihiko</creatorcontrib><creatorcontrib>Kitamura, Yohei</creatorcontrib><creatorcontrib>Shimizu, Takashi</creatorcontrib><creatorcontrib>Beppu, Fumiaki</creatorcontrib><creatorcontrib>Gotoh, Naohiro</creatorcontrib><creatorcontrib>Department of Food Science and Technology</creatorcontrib><creatorcontrib>Tsukishima Foods Industry Co</creatorcontrib><creatorcontrib>Tokyo University of Marine Science and Technology</creatorcontrib><creatorcontrib>Morinaga Milk Industry Co</creatorcontrib><creatorcontrib>Ltd</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Journal of Oleo Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watanabe, Natsuko</au><au>Nagai, Toshiharu</au><au>Mizobe, Hoyo</au><au>Yoshinaga, Kazuaki</au><au>Yoshida, Akihiko</au><au>Kitamura, Yohei</au><au>Shimizu, Takashi</au><au>Beppu, Fumiaki</au><au>Gotoh, Naohiro</au><aucorp>Department of Food Science and Technology</aucorp><aucorp>Tsukishima Foods Industry Co</aucorp><aucorp>Tokyo University of Marine Science and Technology</aucorp><aucorp>Morinaga Milk Industry Co</aucorp><aucorp>Ltd</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of Triacylglycerol Positional Isomers in Rat Milk</atitle><jtitle>Journal of Oleo Science</jtitle><addtitle>J Oleo Sci</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>65</volume><issue>12</issue><spage>977</spage><epage>983</epage><pages>977-983</pages><issn>1345-8957</issn><eissn>1347-3352</eissn><abstract>The absolute amount of triacylglycerol (TAG) positional isomers was analyzed in rat milk fat, a representative of non-ruminant milk fat, using a HPLC-UV-atmospheric pressure chemical ionization-MS/MS system equipped with an octacosyl silylation column or polymeric ODS column. TAGs consisting of two oleic acids (O) and one palmitic acid (P) were the most abundant. In particular, β-OPO, a TAG binding P at the β-position (sn-2) and two Os at the α-positions (sn-1/3), was prominent. The β-OPO content decreased over time, while a TAG consisting of two Ps and one capric acid, a medium-chain fatty acid, increased. TAGs consisting of two Ps and one docosahexaenoic acid were present in small amounts and decreased with time. These results indicated that the recombination of fatty acids in TAGs in milk fat occurs in the mother, and is thought to depend on the infant’s stage of growth, in response to their nutritional needs. It was also demonstrated that medium-chain fatty acids were mainly located at the α-position (sn-3), while Ps were mainly located at the β-position (sn-2). 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subjects | Animal fat Animals Binding Docosahexaenoic acid Fats - chemistry Fatty acids Fatty Acids - analysis infant nutrition Infants Isomers Milk Milk - chemistry milk fat Nutrition Oils & fats Palmitic acid quantification Rats Stereoisomerism Tags triacylglycerol positional isomer Triglycerides Triglycerides - analysis Triglycerides - chemistry |
title | Quantification of Triacylglycerol Positional Isomers in Rat Milk |
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