Quantification of Triacylglycerol Positional Isomers in Rat Milk

The absolute amount of triacylglycerol (TAG) positional isomers was analyzed in rat milk fat, a representative of non-ruminant milk fat, using a HPLC-UV-atmospheric pressure chemical ionization-MS/MS system equipped with an octacosyl silylation column or polymeric ODS column. TAGs consisting of two...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of Oleo Science 2016, Vol.65(12), pp.977-983
Hauptverfasser: Watanabe, Natsuko, Nagai, Toshiharu, Mizobe, Hoyo, Yoshinaga, Kazuaki, Yoshida, Akihiko, Kitamura, Yohei, Shimizu, Takashi, Beppu, Fumiaki, Gotoh, Naohiro
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 983
container_issue 12
container_start_page 977
container_title Journal of Oleo Science
container_volume 65
creator Watanabe, Natsuko
Nagai, Toshiharu
Mizobe, Hoyo
Yoshinaga, Kazuaki
Yoshida, Akihiko
Kitamura, Yohei
Shimizu, Takashi
Beppu, Fumiaki
Gotoh, Naohiro
description The absolute amount of triacylglycerol (TAG) positional isomers was analyzed in rat milk fat, a representative of non-ruminant milk fat, using a HPLC-UV-atmospheric pressure chemical ionization-MS/MS system equipped with an octacosyl silylation column or polymeric ODS column. TAGs consisting of two oleic acids (O) and one palmitic acid (P) were the most abundant. In particular, β-OPO, a TAG binding P at the β-position (sn-2) and two Os at the α-positions (sn-1/3), was prominent. The β-OPO content decreased over time, while a TAG consisting of two Ps and one capric acid, a medium-chain fatty acid, increased. TAGs consisting of two Ps and one docosahexaenoic acid were present in small amounts and decreased with time. These results indicated that the recombination of fatty acids in TAGs in milk fat occurs in the mother, and is thought to depend on the infant’s stage of growth, in response to their nutritional needs. It was also demonstrated that medium-chain fatty acids were mainly located at the α-position (sn-3), while Ps were mainly located at the β-position (sn-2). Therefore, the combination and binding positions of fatty acids of TAG are considered very important in infant nutrition.
doi_str_mv 10.5650/jos.ess16115
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1846132150</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1846132150</sourcerecordid><originalsourceid>FETCH-LOGICAL-c622t-b9f236c32a720edd23653a3dccb0fd7c28ce43cd9035322b99c2997657dd63083</originalsourceid><addsrcrecordid>eNpFUE1PAyEUJEbj982z2cSrq_AosNw0jVYTjR_RM6HAKitdFLaH_nvR2np5D5h584ZB6IjgM8YZPu9iPnM5E04I20C7hI5ETSmDzd8zqxvJxA7ay7nDuLwzsY12QDQgOaG76OJprvvBt97owce-im31krw2i_AWFsalGKrHmP0PpkN1m-PMpVz5vnrWQ3Xvw8cB2mp1yO7wr--j1-url_FNffcwuR1f3tWGAwz1VLZAuaGgBWBnbbkwqqk1ZopbKww0xo2osRJTRgGmUhqQUnAmrOUUN3QfnSx1P1P8mrs8qC7OUzGVFWlG5S9AGC6s0yXLpJhzcq36TH6m00IRrH7iKlNZreIq9OM_0fl05uyavMqnECZLQkFLRCH2wffuf7XtRAyuSAImXGHMGYHSqMJSiFIaCoJzYLIojZdKXR70m1uv0mnwJrhfX5ypMl7qyuAaNe86KdfTbwejlMs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1846132150</pqid></control><display><type>article</type><title>Quantification of Triacylglycerol Positional Isomers in Rat Milk</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>J-STAGE (Japan Science &amp; Technology Information Aggregator, Electronic) Freely Available Titles - Japanese</source><source>Free Full-Text Journals in Chemistry</source><creator>Watanabe, Natsuko ; Nagai, Toshiharu ; Mizobe, Hoyo ; Yoshinaga, Kazuaki ; Yoshida, Akihiko ; Kitamura, Yohei ; Shimizu, Takashi ; Beppu, Fumiaki ; Gotoh, Naohiro</creator><creatorcontrib>Watanabe, Natsuko ; Nagai, Toshiharu ; Mizobe, Hoyo ; Yoshinaga, Kazuaki ; Yoshida, Akihiko ; Kitamura, Yohei ; Shimizu, Takashi ; Beppu, Fumiaki ; Gotoh, Naohiro ; Department of Food Science and Technology ; Tsukishima Foods Industry Co ; Tokyo University of Marine Science and Technology ; Morinaga Milk Industry Co ; Ltd</creatorcontrib><description>The absolute amount of triacylglycerol (TAG) positional isomers was analyzed in rat milk fat, a representative of non-ruminant milk fat, using a HPLC-UV-atmospheric pressure chemical ionization-MS/MS system equipped with an octacosyl silylation column or polymeric ODS column. TAGs consisting of two oleic acids (O) and one palmitic acid (P) were the most abundant. In particular, β-OPO, a TAG binding P at the β-position (sn-2) and two Os at the α-positions (sn-1/3), was prominent. The β-OPO content decreased over time, while a TAG consisting of two Ps and one capric acid, a medium-chain fatty acid, increased. TAGs consisting of two Ps and one docosahexaenoic acid were present in small amounts and decreased with time. These results indicated that the recombination of fatty acids in TAGs in milk fat occurs in the mother, and is thought to depend on the infant’s stage of growth, in response to their nutritional needs. It was also demonstrated that medium-chain fatty acids were mainly located at the α-position (sn-3), while Ps were mainly located at the β-position (sn-2). Therefore, the combination and binding positions of fatty acids of TAG are considered very important in infant nutrition.</description><identifier>ISSN: 1345-8957</identifier><identifier>EISSN: 1347-3352</identifier><identifier>DOI: 10.5650/jos.ess16115</identifier><identifier>PMID: 27829613</identifier><language>eng</language><publisher>Japan: Japan Oil Chemists' Society</publisher><subject>Animal fat ; Animals ; Binding ; Docosahexaenoic acid ; Fats - chemistry ; Fatty acids ; Fatty Acids - analysis ; infant nutrition ; Infants ; Isomers ; Milk ; Milk - chemistry ; milk fat ; Nutrition ; Oils &amp; fats ; Palmitic acid ; quantification ; Rats ; Stereoisomerism ; Tags ; triacylglycerol positional isomer ; Triglycerides ; Triglycerides - analysis ; Triglycerides - chemistry</subject><ispartof>Journal of Oleo Science, 2016, Vol.65(12), pp.977-983</ispartof><rights>2016 by Japan Oil Chemists' Society</rights><rights>Copyright Japan Science and Technology Agency 2016</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c622t-b9f236c32a720edd23653a3dccb0fd7c28ce43cd9035322b99c2997657dd63083</citedby><cites>FETCH-LOGICAL-c622t-b9f236c32a720edd23653a3dccb0fd7c28ce43cd9035322b99c2997657dd63083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27829613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watanabe, Natsuko</creatorcontrib><creatorcontrib>Nagai, Toshiharu</creatorcontrib><creatorcontrib>Mizobe, Hoyo</creatorcontrib><creatorcontrib>Yoshinaga, Kazuaki</creatorcontrib><creatorcontrib>Yoshida, Akihiko</creatorcontrib><creatorcontrib>Kitamura, Yohei</creatorcontrib><creatorcontrib>Shimizu, Takashi</creatorcontrib><creatorcontrib>Beppu, Fumiaki</creatorcontrib><creatorcontrib>Gotoh, Naohiro</creatorcontrib><creatorcontrib>Department of Food Science and Technology</creatorcontrib><creatorcontrib>Tsukishima Foods Industry Co</creatorcontrib><creatorcontrib>Tokyo University of Marine Science and Technology</creatorcontrib><creatorcontrib>Morinaga Milk Industry Co</creatorcontrib><creatorcontrib>Ltd</creatorcontrib><title>Quantification of Triacylglycerol Positional Isomers in Rat Milk</title><title>Journal of Oleo Science</title><addtitle>J Oleo Sci</addtitle><description>The absolute amount of triacylglycerol (TAG) positional isomers was analyzed in rat milk fat, a representative of non-ruminant milk fat, using a HPLC-UV-atmospheric pressure chemical ionization-MS/MS system equipped with an octacosyl silylation column or polymeric ODS column. TAGs consisting of two oleic acids (O) and one palmitic acid (P) were the most abundant. In particular, β-OPO, a TAG binding P at the β-position (sn-2) and two Os at the α-positions (sn-1/3), was prominent. The β-OPO content decreased over time, while a TAG consisting of two Ps and one capric acid, a medium-chain fatty acid, increased. TAGs consisting of two Ps and one docosahexaenoic acid were present in small amounts and decreased with time. These results indicated that the recombination of fatty acids in TAGs in milk fat occurs in the mother, and is thought to depend on the infant’s stage of growth, in response to their nutritional needs. It was also demonstrated that medium-chain fatty acids were mainly located at the α-position (sn-3), while Ps were mainly located at the β-position (sn-2). Therefore, the combination and binding positions of fatty acids of TAG are considered very important in infant nutrition.</description><subject>Animal fat</subject><subject>Animals</subject><subject>Binding</subject><subject>Docosahexaenoic acid</subject><subject>Fats - chemistry</subject><subject>Fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>infant nutrition</subject><subject>Infants</subject><subject>Isomers</subject><subject>Milk</subject><subject>Milk - chemistry</subject><subject>milk fat</subject><subject>Nutrition</subject><subject>Oils &amp; fats</subject><subject>Palmitic acid</subject><subject>quantification</subject><subject>Rats</subject><subject>Stereoisomerism</subject><subject>Tags</subject><subject>triacylglycerol positional isomer</subject><subject>Triglycerides</subject><subject>Triglycerides - analysis</subject><subject>Triglycerides - chemistry</subject><issn>1345-8957</issn><issn>1347-3352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUE1PAyEUJEbj982z2cSrq_AosNw0jVYTjR_RM6HAKitdFLaH_nvR2np5D5h584ZB6IjgM8YZPu9iPnM5E04I20C7hI5ETSmDzd8zqxvJxA7ay7nDuLwzsY12QDQgOaG76OJprvvBt97owce-im31krw2i_AWFsalGKrHmP0PpkN1m-PMpVz5vnrWQ3Xvw8cB2mp1yO7wr--j1-url_FNffcwuR1f3tWGAwz1VLZAuaGgBWBnbbkwqqk1ZopbKww0xo2osRJTRgGmUhqQUnAmrOUUN3QfnSx1P1P8mrs8qC7OUzGVFWlG5S9AGC6s0yXLpJhzcq36TH6m00IRrH7iKlNZreIq9OM_0fl05uyavMqnECZLQkFLRCH2wffuf7XtRAyuSAImXGHMGYHSqMJSiFIaCoJzYLIojZdKXR70m1uv0mnwJrhfX5ypMl7qyuAaNe86KdfTbwejlMs</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Watanabe, Natsuko</creator><creator>Nagai, Toshiharu</creator><creator>Mizobe, Hoyo</creator><creator>Yoshinaga, Kazuaki</creator><creator>Yoshida, Akihiko</creator><creator>Kitamura, Yohei</creator><creator>Shimizu, Takashi</creator><creator>Beppu, Fumiaki</creator><creator>Gotoh, Naohiro</creator><general>Japan Oil Chemists' Society</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>20161201</creationdate><title>Quantification of Triacylglycerol Positional Isomers in Rat Milk</title><author>Watanabe, Natsuko ; Nagai, Toshiharu ; Mizobe, Hoyo ; Yoshinaga, Kazuaki ; Yoshida, Akihiko ; Kitamura, Yohei ; Shimizu, Takashi ; Beppu, Fumiaki ; Gotoh, Naohiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c622t-b9f236c32a720edd23653a3dccb0fd7c28ce43cd9035322b99c2997657dd63083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animal fat</topic><topic>Animals</topic><topic>Binding</topic><topic>Docosahexaenoic acid</topic><topic>Fats - chemistry</topic><topic>Fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>infant nutrition</topic><topic>Infants</topic><topic>Isomers</topic><topic>Milk</topic><topic>Milk - chemistry</topic><topic>milk fat</topic><topic>Nutrition</topic><topic>Oils &amp; fats</topic><topic>Palmitic acid</topic><topic>quantification</topic><topic>Rats</topic><topic>Stereoisomerism</topic><topic>Tags</topic><topic>triacylglycerol positional isomer</topic><topic>Triglycerides</topic><topic>Triglycerides - analysis</topic><topic>Triglycerides - chemistry</topic><toplevel>online_resources</toplevel><creatorcontrib>Watanabe, Natsuko</creatorcontrib><creatorcontrib>Nagai, Toshiharu</creatorcontrib><creatorcontrib>Mizobe, Hoyo</creatorcontrib><creatorcontrib>Yoshinaga, Kazuaki</creatorcontrib><creatorcontrib>Yoshida, Akihiko</creatorcontrib><creatorcontrib>Kitamura, Yohei</creatorcontrib><creatorcontrib>Shimizu, Takashi</creatorcontrib><creatorcontrib>Beppu, Fumiaki</creatorcontrib><creatorcontrib>Gotoh, Naohiro</creatorcontrib><creatorcontrib>Department of Food Science and Technology</creatorcontrib><creatorcontrib>Tsukishima Foods Industry Co</creatorcontrib><creatorcontrib>Tokyo University of Marine Science and Technology</creatorcontrib><creatorcontrib>Morinaga Milk Industry Co</creatorcontrib><creatorcontrib>Ltd</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology &amp; Engineering</collection><collection>Engineering Research Database</collection><jtitle>Journal of Oleo Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watanabe, Natsuko</au><au>Nagai, Toshiharu</au><au>Mizobe, Hoyo</au><au>Yoshinaga, Kazuaki</au><au>Yoshida, Akihiko</au><au>Kitamura, Yohei</au><au>Shimizu, Takashi</au><au>Beppu, Fumiaki</au><au>Gotoh, Naohiro</au><aucorp>Department of Food Science and Technology</aucorp><aucorp>Tsukishima Foods Industry Co</aucorp><aucorp>Tokyo University of Marine Science and Technology</aucorp><aucorp>Morinaga Milk Industry Co</aucorp><aucorp>Ltd</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of Triacylglycerol Positional Isomers in Rat Milk</atitle><jtitle>Journal of Oleo Science</jtitle><addtitle>J Oleo Sci</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>65</volume><issue>12</issue><spage>977</spage><epage>983</epage><pages>977-983</pages><issn>1345-8957</issn><eissn>1347-3352</eissn><abstract>The absolute amount of triacylglycerol (TAG) positional isomers was analyzed in rat milk fat, a representative of non-ruminant milk fat, using a HPLC-UV-atmospheric pressure chemical ionization-MS/MS system equipped with an octacosyl silylation column or polymeric ODS column. TAGs consisting of two oleic acids (O) and one palmitic acid (P) were the most abundant. In particular, β-OPO, a TAG binding P at the β-position (sn-2) and two Os at the α-positions (sn-1/3), was prominent. The β-OPO content decreased over time, while a TAG consisting of two Ps and one capric acid, a medium-chain fatty acid, increased. TAGs consisting of two Ps and one docosahexaenoic acid were present in small amounts and decreased with time. These results indicated that the recombination of fatty acids in TAGs in milk fat occurs in the mother, and is thought to depend on the infant’s stage of growth, in response to their nutritional needs. It was also demonstrated that medium-chain fatty acids were mainly located at the α-position (sn-3), while Ps were mainly located at the β-position (sn-2). Therefore, the combination and binding positions of fatty acids of TAG are considered very important in infant nutrition.</abstract><cop>Japan</cop><pub>Japan Oil Chemists' Society</pub><pmid>27829613</pmid><doi>10.5650/jos.ess16115</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1345-8957
ispartof Journal of Oleo Science, 2016, Vol.65(12), pp.977-983
issn 1345-8957
1347-3352
language eng
recordid cdi_proquest_journals_1846132150
source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Free Full-Text Journals in Chemistry
subjects Animal fat
Animals
Binding
Docosahexaenoic acid
Fats - chemistry
Fatty acids
Fatty Acids - analysis
infant nutrition
Infants
Isomers
Milk
Milk - chemistry
milk fat
Nutrition
Oils & fats
Palmitic acid
quantification
Rats
Stereoisomerism
Tags
triacylglycerol positional isomer
Triglycerides
Triglycerides - analysis
Triglycerides - chemistry
title Quantification of Triacylglycerol Positional Isomers in Rat Milk
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T03%3A50%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantification%20of%20Triacylglycerol%20Positional%20Isomers%20in%20Rat%20Milk&rft.jtitle=Journal%20of%20Oleo%20Science&rft.au=Watanabe,%20Natsuko&rft.aucorp=Department%20of%20Food%20Science%20and%20Technology&rft.date=2016-12-01&rft.volume=65&rft.issue=12&rft.spage=977&rft.epage=983&rft.pages=977-983&rft.issn=1345-8957&rft.eissn=1347-3352&rft_id=info:doi/10.5650/jos.ess16115&rft_dat=%3Cproquest_cross%3E1846132150%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1846132150&rft_id=info:pmid/27829613&rfr_iscdi=true