Homogeneous electrochemical immunoassay of aflatoxin B^sub 1^ in foodstuff using proximity-hybridization-induced omega-like DNA junctions and exonuclease III-triggered isothermal cycling signal amplification

A new homogeneous electrochemical immunosensing platform was designed for sensitive detection of aflatoxin B1 (AFB1) in foodstuff. The system consisted of anti-AFB1 antibody labeled DNA1 (Ab-DNA1), AFB1-bovine serum albumin (BSA)-conjugated DNA2 (AFB1-DNA2), and methylene blue functionalized hairpin...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2016-12, Vol.408 (30), p.8593
Hauptverfasser:	Tang, Juan, Huang, Yapei, Liu, Huiqiong, Zhang, Cengceng, Tang, Dianping
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Aflatoxins Agricultural commodities Carcinogens Deoxyribonucleic acid Design DNA Electrochemistry Electrodes Enzymes Hybridization Immunoassay Signal transduction Thermal cycling
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