Isolation, characterisation and determination of biological activity of coffee proanthocyanidins
Two proanthocyanidin‐containing preparations were isolated from the pulp of ripe Coffea canephora coffee cherries. The preparations were partially purified by Sephadex LH‐20 column chromatography and characterised by means of infrared and 13C NMR spectroscopy, and reactions with several functional g...
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| Veröffentlicht in: | Journal of the science of food and agriculture 1998-07, Vol.77 (3), p.368-372 |
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| container_title | Journal of the science of food and agriculture |
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| creator | de Colmenares, Nélida González Ramírez-Martínez, José R Aldana, José O Ramos-Niño, María E Clifford, Michael N Pékerar, Sara Méndez, Bernardo |
| description | Two proanthocyanidin‐containing preparations were isolated from the pulp of ripe Coffea canephora coffee cherries. The preparations were partially purified by Sephadex LH‐20 column chromatography and characterised by means of infrared and 13C NMR spectroscopy, and reactions with several functional group‐specific reagents. These proanthocyanidin‐rich extracts from coffee pulp, obtained also from coffee leaves, inhibit the germination in vitro of Hemileia vastatrix race 2 uredospores. Greater proanthocyanidin content was associated with greater potency, and the leaves of Coffea arabica, a coffee species susceptible to rust, contained less proanthocyanidins than the leaves of C canephora, a rust‐resistant coffee. © 1998 SCI. |
| doi_str_mv | 10.1002/(SICI)1097-0010(199807)77:3<368::AID-JSFA52>3.0.CO;2-V |
| format | Article |
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The preparations were partially purified by Sephadex LH‐20 column chromatography and characterised by means of infrared and 13C NMR spectroscopy, and reactions with several functional group‐specific reagents. These proanthocyanidin‐rich extracts from coffee pulp, obtained also from coffee leaves, inhibit the germination in vitro of Hemileia vastatrix race 2 uredospores. Greater proanthocyanidin content was associated with greater potency, and the leaves of Coffea arabica, a coffee species susceptible to rust, contained less proanthocyanidins than the leaves of C canephora, a rust‐resistant coffee. © 1998 SCI.</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/(SICI)1097-0010(199807)77:3<368::AID-JSFA52>3.0.CO;2-V</identifier><identifier>CODEN: JSFAAE</identifier><language>eng</language><publisher>London: John Wiley & Sons, Ltd</publisher><subject>antifungal activity ; Biological and medical sciences ; coffee pulp ; coffee rust ; condensed tannins ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Hemileia vastatrix ; Pathology, epidemiology, host-fungus relationships. Damages, economic importance ; Phytopathology. Animal pests. 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Sci. Food Agric</addtitle><description>Two proanthocyanidin‐containing preparations were isolated from the pulp of ripe Coffea canephora coffee cherries. The preparations were partially purified by Sephadex LH‐20 column chromatography and characterised by means of infrared and 13C NMR spectroscopy, and reactions with several functional group‐specific reagents. These proanthocyanidin‐rich extracts from coffee pulp, obtained also from coffee leaves, inhibit the germination in vitro of Hemileia vastatrix race 2 uredospores. Greater proanthocyanidin content was associated with greater potency, and the leaves of Coffea arabica, a coffee species susceptible to rust, contained less proanthocyanidins than the leaves of C canephora, a rust‐resistant coffee. © 1998 SCI.</description><subject>antifungal activity</subject><subject>Biological and medical sciences</subject><subject>coffee pulp</subject><subject>coffee rust</subject><subject>condensed tannins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Hemileia vastatrix</subject><subject>Pathology, epidemiology, host-fungus relationships. Damages, economic importance</subject><subject>Phytopathology. Animal pests. 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Psychology</topic><topic>Fungal plant pathogens</topic><topic>Hemileia vastatrix</topic><topic>Pathology, epidemiology, host-fungus relationships. Damages, economic importance</topic><topic>Phytopathology. Animal pests. 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Sci. Food Agric</addtitle><date>1998-07</date><risdate>1998</risdate><volume>77</volume><issue>3</issue><spage>368</spage><epage>372</epage><pages>368-372</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><coden>JSFAAE</coden><abstract>Two proanthocyanidin‐containing preparations were isolated from the pulp of ripe Coffea canephora coffee cherries. The preparations were partially purified by Sephadex LH‐20 column chromatography and characterised by means of infrared and 13C NMR spectroscopy, and reactions with several functional group‐specific reagents. These proanthocyanidin‐rich extracts from coffee pulp, obtained also from coffee leaves, inhibit the germination in vitro of Hemileia vastatrix race 2 uredospores. Greater proanthocyanidin content was associated with greater potency, and the leaves of Coffea arabica, a coffee species susceptible to rust, contained less proanthocyanidins than the leaves of C canephora, a rust‐resistant coffee. © 1998 SCI.</abstract><cop>London</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/(SICI)1097-0010(199807)77:3<368::AID-JSFA52>3.0.CO;2-V</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of the science of food and agriculture, 1998-07, Vol.77 (3), p.368-372 |
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| source | Wiley Online Library Journals Frontfile Complete; Periodicals Index Online |
| subjects | antifungal activity Biological and medical sciences coffee pulp coffee rust condensed tannins Fundamental and applied biological sciences. Psychology Fungal plant pathogens Hemileia vastatrix Pathology, epidemiology, host-fungus relationships. Damages, economic importance Phytopathology. Animal pests. Plant and forest protection proanthocyanidins |
| title | Isolation, characterisation and determination of biological activity of coffee proanthocyanidins |
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