Lentiviral Vector-Mediated p27^sup kip1^ Expression Facilitates Recovery After Spinal Cord Injury
Traumatic spinal cord injury (SCI) causes tissue loss and associated neurological dysfunction attributable to both mechanical damage and secondary biochemical and physiological responses. Upregulation of cell cycle proteins occurs in both neurons and glia after SCI and may contribute to these change...
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| Veröffentlicht in: | Molecular neurobiology 2016-11, Vol.53 (9), p.6043 |
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| container_title | Molecular neurobiology |
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| creator | Chen, Min-hao Liu, Yong-hua Xu, Hua Xu, Da-wei Wang, Cheng-niu Wang, Yi Duan, Cheng-wei Zhou, Ying Kan, Peng Shen, Ai-guo Wang, You-hua |
| description | Traumatic spinal cord injury (SCI) causes tissue loss and associated neurological dysfunction attributable to both mechanical damage and secondary biochemical and physiological responses. Upregulation of cell cycle proteins occurs in both neurons and glia after SCI and may contribute to these changes. Increased cell cycle protein is associated with neuronal and oligodendroglial apoptosis, reactive astrogliosis, glial scar formation, and microglial activation. Here, using lentiviral vectors (LV), we induced the expression of the cyclin-dependent kinase (CDK) inhibitor p27kip1 in the lesioned spinal cord of adult rat. Treatment with LV-p27kip1 significantly reduced the expression of cell cycle proteins and improved functional recovery. In addition, p27kip1 overexpression also reduced lesion volume, decreased astrocytic reactivity, attenuated microglial activation, reduced cell death, and improved the local microenvironment. We suggest that these effects reflect the ability of p27kip1 to inhibit cell cycle pathways. Thus, the present study provides further support for the therapeutic potential of cell cycle inhibitors in the treatment of SCI. |
| doi_str_mv | 10.1007/s12035-015-9498-2 |
| format | Article |
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Upregulation of cell cycle proteins occurs in both neurons and glia after SCI and may contribute to these changes. Increased cell cycle protein is associated with neuronal and oligodendroglial apoptosis, reactive astrogliosis, glial scar formation, and microglial activation. Here, using lentiviral vectors (LV), we induced the expression of the cyclin-dependent kinase (CDK) inhibitor p27kip1 in the lesioned spinal cord of adult rat. Treatment with LV-p27kip1 significantly reduced the expression of cell cycle proteins and improved functional recovery. In addition, p27kip1 overexpression also reduced lesion volume, decreased astrocytic reactivity, attenuated microglial activation, reduced cell death, and improved the local microenvironment. We suggest that these effects reflect the ability of p27kip1 to inhibit cell cycle pathways. Thus, the present study provides further support for the therapeutic potential of cell cycle inhibitors in the treatment of SCI.</description><identifier>ISSN: 0893-7648</identifier><identifier>EISSN: 1559-1182</identifier><identifier>DOI: 10.1007/s12035-015-9498-2</identifier><language>eng</language><publisher>Totowa: Springer Nature B.V</publisher><subject>Apoptosis ; Cell cycle ; Cell growth ; Neurobiology ; Spinal cord injuries</subject><ispartof>Molecular neurobiology, 2016-11, Vol.53 (9), p.6043</ispartof><rights>Springer Science+Business Media New York 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Chen, Min-hao</creatorcontrib><creatorcontrib>Liu, Yong-hua</creatorcontrib><creatorcontrib>Xu, Hua</creatorcontrib><creatorcontrib>Xu, Da-wei</creatorcontrib><creatorcontrib>Wang, Cheng-niu</creatorcontrib><creatorcontrib>Wang, Yi</creatorcontrib><creatorcontrib>Duan, Cheng-wei</creatorcontrib><creatorcontrib>Zhou, Ying</creatorcontrib><creatorcontrib>Kan, Peng</creatorcontrib><creatorcontrib>Shen, Ai-guo</creatorcontrib><creatorcontrib>Wang, You-hua</creatorcontrib><title>Lentiviral Vector-Mediated p27^sup kip1^ Expression Facilitates Recovery After Spinal Cord Injury</title><title>Molecular neurobiology</title><description>Traumatic spinal cord injury (SCI) causes tissue loss and associated neurological dysfunction attributable to both mechanical damage and secondary biochemical and physiological responses. Upregulation of cell cycle proteins occurs in both neurons and glia after SCI and may contribute to these changes. Increased cell cycle protein is associated with neuronal and oligodendroglial apoptosis, reactive astrogliosis, glial scar formation, and microglial activation. Here, using lentiviral vectors (LV), we induced the expression of the cyclin-dependent kinase (CDK) inhibitor p27kip1 in the lesioned spinal cord of adult rat. Treatment with LV-p27kip1 significantly reduced the expression of cell cycle proteins and improved functional recovery. In addition, p27kip1 overexpression also reduced lesion volume, decreased astrocytic reactivity, attenuated microglial activation, reduced cell death, and improved the local microenvironment. We suggest that these effects reflect the ability of p27kip1 to inhibit cell cycle pathways. Thus, the present study provides further support for the therapeutic potential of cell cycle inhibitors in the treatment of SCI.</description><subject>Apoptosis</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Neurobiology</subject><subject>Spinal cord injuries</subject><issn>0893-7648</issn><issn>1559-1182</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqNjMFKAzEUAEOx0NX6Ad4CnqN5SdNNjlJaLOhFxWNL2H2FrMsm5mWL_Xv34Ad4msvMMHYH8gGkrB8JlNRGSDDCrZwVasYqMMYJAKuuWCWt06Jer-yCXRN1UioFsq6Yf8GhhHPIvuef2JSYxSu2wRdseVL1gcbEv0KCA9_-pIxEIQ5855vQhzJJxN-wiWfMF_50Kpj5ewrDtNrE3PL90I35smTzk-8Jb_94w-5324_Ns0g5fo9I5djFMU8RHcFqDcbBWuv_Wb8ppkt8</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Chen, Min-hao</creator><creator>Liu, Yong-hua</creator><creator>Xu, Hua</creator><creator>Xu, Da-wei</creator><creator>Wang, Cheng-niu</creator><creator>Wang, Yi</creator><creator>Duan, Cheng-wei</creator><creator>Zhou, Ying</creator><creator>Kan, Peng</creator><creator>Shen, Ai-guo</creator><creator>Wang, You-hua</creator><general>Springer Nature B.V</general><scope>3V.</scope><scope>7QR</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88G</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope></search><sort><creationdate>20161101</creationdate><title>Lentiviral Vector-Mediated p27^sup kip1^ Expression Facilitates Recovery After Spinal Cord Injury</title><author>Chen, Min-hao ; 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| subjects | Apoptosis Cell cycle Cell growth Neurobiology Spinal cord injuries |
| title | Lentiviral Vector-Mediated p27^sup kip1^ Expression Facilitates Recovery After Spinal Cord Injury |
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